Rmu_sc0002209.1_g000021

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002209.1
OG0004557
Physical Location & Seq
Reverse (-)
80205 .. 82889
2685 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
atggatcgcctggagcgcatgacgtcctcctcgacgatatccaagggaaggacgacagtggggtcgacccggagggcgatctgccaggtcagcagggaggggaagagcaaggaagccgaggaaggcttccggcgactccggaggaggagggaaggtgaacaggtcgggcagatcggcggcgaccttcctcctggctctcttggtagcctcgttcacaaccatggtagatctgagctttgtcgaaaaaaaccatgtgaagctttggaaactagcagccatgtgctatgcaactgtcctatggctcaagaagcaattggagcagcaaatattccagttgctcagctgctagcaattagagagggcatcgagtttctacagcacaggggcctccaagacgcttgccttgaaagtgactgccaagttgccatacaagagattgaagaagaagattttgatctcaatgagaatgccaatttgttgttggatatacaacagtgcagtaagatgatgcacaatctgacgttcacatttgctcctagaactgccaattttgtggcacatggattagcagccattgccaatgaatccaatactactcaagtatggatagaccaagctccagaaagcattatggatgtcttagctttgattgtaattgctccatttaatcaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

224

Amino Acids

24.68

Weight (kDa)

5.43

Isoelectric Point (pI)

59.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 104 - 191 3.8e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
AatII GACGTC 1 cut(s) 26
AccI GTMKAC 1 cut(s) 65
AccIII TCCGGA 1 cut(s) 138
AciI CCGC 1 cut(s) 177
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 23
AfiI CCNNNNNNNGG 1 cut(s) 48
AgsI TTSAA 2 cut(s) 407, 440
AjnI CCWGG 3 cut(s) 9, 84, 190
AluBI AGCT 5 cut(s) 235, 260, 343, 617, 644
AluI AGCT 5 cut(s) 235, 260, 343, 617, 644
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 138
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 4 cut(s) 273, 320, 343, 569
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 385
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 167
AsuNHI GCTAGC 1 cut(s) 346
BbvI GCAGC 4 cut(s) 285, 330, 332, 581
BciT130I CCWGG 3 cut(s) 11, 86, 192
BcnI CCSGG 1 cut(s) 70
BfaI CTAG 3 cut(s) 270, 347, 537
BfmI CTRYAG 1 cut(s) 374
BglII AGATCT 1 cut(s) 227
BisI GCNGC 5 cut(s) 178, 274, 321, 344, 570
BlpI GCTNAGC 1 cut(s) 339
BlsI GCNGC 5 cut(s) 179, 275, 322, 345, 571
Bme1390I CCNGG 4 cut(s) 11, 70, 86, 192
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 386
BmrFI CCNGG 4 cut(s) 11, 70, 86, 192
BmsI GCATC 2 cut(s) 372, 498
BmtI GCTAGC 1 cut(s) 350
BpmI CTGGAG 2 cut(s) 32, 603
Bpu1102I GCTNAGC 1 cut(s) 339
BpuEI CTTGAG 2 cut(s) 288, 582
BpuMI CCSGG 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 3 cut(s) 42, 117, 220
BsaWI WCCGGW 1 cut(s) 138
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 517, 547
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 1 cut(s) 332
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 453
BseAI TCCGGA 1 cut(s) 138
BseBI CCWGG 3 cut(s) 11, 86, 192
BseDI CCNNGG 3 cut(s) 42, 117, 220
BseGI GGATG 1 cut(s) 640
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 2 cut(s) 222, 353
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 3 cut(s) 19, 157, 160
BseXI GCAGC 4 cut(s) 285, 330, 332, 581
BsgI GTGCAG 1 cut(s) 517
BshFI GGCC 1 cut(s) 387
BsiSI CCGG 3 cut(s) 70, 130, 139
BslI CCNNNNNNNGG 1 cut(s) 48
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 1 cut(s) 387
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 5 cut(s) 4, 78, 171, 227, 454
Bsp1720I GCTNAGC 1 cut(s) 339
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 1 cut(s) 177
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 2 cut(s) 223, 352
BspEI TCCGGA 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 386
BspOI GCTAGC 1 cut(s) 350
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 1 cut(s) 332
BssECI CCNNGG 3 cut(s) 42, 117, 220
BssMI GATC 5 cut(s) 4, 78, 171, 227, 454
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 2 cut(s) 42, 220
Bst2UI CCWGG 3 cut(s) 11, 86, 192
Bst4CI ACNGT 3 cut(s) 58, 293, 495
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 575
BstC8I GCNNGC 2 cut(s) 348, 400
BstDEI CTNAG 3 cut(s) 231, 339, 640
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 1 cut(s) 640
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 5 cut(s) 7, 81, 174, 230, 457
BstMBI GATC 5 cut(s) 4, 78, 171, 227, 454
BstMWI GCNNNNNNNGC 5 cut(s) 15, 90, 308, 317, 575
BstNI CCWGG 3 cut(s) 11, 86, 192
BstSCI CCNGG 4 cut(s) 9, 68, 84, 190
BstSFI CTRYAG 1 cut(s) 374
BstV1I GCAGC 4 cut(s) 285, 330, 332, 581
BstX2I RGATCY 1 cut(s) 227
BstXI CCANNNNNNTGG 1 cut(s) 553
BstYI RGATCY 1 cut(s) 227
BsuRI GGCC 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 1 cut(s) 640
BtsIMutI CAGTG 2 cut(s) 63, 500
Cac8I GCNNGC 2 cut(s) 348, 400
CfoI GCGC 1 cut(s) 18
Cfr13I GGNCC 1 cut(s) 385
CseI GACGC 1 cut(s) 404
CviAII CATG 5 cut(s) 19, 221, 252, 278, 560
DdeI CTNAG 3 cut(s) 231, 339, 640
DpnI GATC 5 cut(s) 6, 80, 173, 229, 456
DpnII GATC 5 cut(s) 4, 78, 171, 227, 454
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 2 cut(s) 42, 220
Eco32I GATATC 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 385
EcoRII CCWGG 3 cut(s) 9, 84, 190
EcoRV GATATC 1 cut(s) 39
EcoT14I CCWWGG 2 cut(s) 42, 220
ErhI CCWWGG 2 cut(s) 42, 220
FaeI CATG 5 cut(s) 22, 224, 255, 281, 563
FatI CATG 5 cut(s) 18, 220, 251, 277, 559
FblI GTMKAC 1 cut(s) 65
Fnu4HI GCNGC 5 cut(s) 178, 274, 321, 344, 570
FokI GGATG 1 cut(s) 647
Fsp4HI GCNGC 5 cut(s) 178, 274, 321, 344, 570
FspBI CTAG 3 cut(s) 270, 347, 537
GlaI GCGC 1 cut(s) 17
GluI GCNGC 5 cut(s) 178, 274, 321, 344, 570
GsuI CTGGAG 2 cut(s) 32, 603
HaeIII GGCC 1 cut(s) 387
HapII CCGG 3 cut(s) 70, 130, 139
HgaI GACGC 1 cut(s) 404
HhaI GCGC 1 cut(s) 18
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 5 cut(s) 22, 224, 255, 281, 563
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 2 cut(s) 135, 584
HpaII CCGG 3 cut(s) 70, 130, 139
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 4 cut(s) 66, 158, 214, 525
Hpy188I TCNGA 2 cut(s) 232, 519
Hpy188III TCNNGA 3 cut(s) 139, 305, 620
Hpy8I GTNNAC 4 cut(s) 66, 158, 214, 525
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 4 cut(s) 42, 116, 146, 194
HpyCH4III ACNGT 3 cut(s) 58, 293, 495
HpyCH4IV ACGT 2 cut(s) 23, 521
HpyCH4V TGCA 3 cut(s) 288, 498, 511
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 90, 308, 317, 575
HpyF3I CTNAG 3 cut(s) 231, 339, 640
HpySE526I ACGT 2 cut(s) 23, 521
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 5 cut(s) 22, 224, 255, 281, 563
HspAI GCGC 1 cut(s) 16
Kpn2I TCCGGA 1 cut(s) 138
Kzo9I GATC 5 cut(s) 4, 78, 171, 227, 454
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 5 cut(s) 13, 317, 538, 622, 664
Lsp1109I GCAGC 4 cut(s) 285, 330, 332, 581
LweI GCATC 2 cut(s) 372, 498
MaeI CTAG 3 cut(s) 270, 347, 537
MaeII ACGT 2 cut(s) 23, 521
MaeIII GTNAC 1 cut(s) 410
MalI GATC 5 cut(s) 6, 80, 173, 229, 456
MboI GATC 5 cut(s) 4, 78, 171, 227, 454
MboII GAAGA 4 cut(s) 115, 452, 455, 458
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 227
MluCI AATT 5 cut(s) 312, 351, 472, 547, 654
MlyI GAGTC 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 462
MroI TCCGGA 1 cut(s) 138
MseI TTAA 1 cut(s) 666
MslI CAYNNNNRTG 1 cut(s) 219
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 3 cut(s) 70, 130, 139
MspR9I CCNGG 4 cut(s) 11, 70, 86, 192
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 472
MvaI CCWGG 3 cut(s) 11, 86, 192
MwoI GCNNNNNNNGC 5 cut(s) 15, 90, 308, 317, 575
NciI CCSGG 1 cut(s) 70
NcoI CCATGG 1 cut(s) 220
NdeII GATC 5 cut(s) 4, 78, 171, 227, 454
NheI GCTAGC 1 cut(s) 346
NlaIII CATG 5 cut(s) 22, 224, 255, 281, 563
NlaIV GGNNCC 1 cut(s) 386
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 1 cut(s) 472
PfeI GAWTC 1 cut(s) 584
PkrI GCNGC 5 cut(s) 179, 275, 322, 345, 571
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
Psp6I CCWGG 3 cut(s) 9, 84, 190
PspGI CCWGG 3 cut(s) 9, 84, 190
PspN4I GGNNCC 1 cut(s) 386
PspPI GGNCC 1 cut(s) 385
PsuI RGATCY 1 cut(s) 227
PvuII CAGCTG 1 cut(s) 343
RseI CAYNNNNRTG 1 cut(s) 219
SalI GTCGAC 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 666
SatI GCNGC 5 cut(s) 178, 274, 321, 344, 570
Sau3AI GATC 5 cut(s) 4, 78, 171, 227, 454
Sau96I GGNCC 1 cut(s) 385
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 4 cut(s) 11, 70, 86, 192
SfaNI GCATC 2 cut(s) 372, 498
SfcI CTRYAG 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 2 cut(s) 303, 597
SmoI CTYRAG 2 cut(s) 303, 597
Sse9I AATT 5 cut(s) 312, 351, 472, 547, 654
SsiI CCGC 1 cut(s) 177
SspI AATATT 1 cut(s) 328
SspMI CTAG 3 cut(s) 270, 347, 537
StyD4I CCNGG 4 cut(s) 9, 68, 84, 190
StyI CCWWGG 2 cut(s) 42, 220
TaaI ACNGT 3 cut(s) 58, 293, 495
TaiI ACGT 2 cut(s) 26, 524
TaqI TCGA 4 cut(s) 32, 65, 241, 366
TasI AATT 5 cut(s) 312, 351, 472, 547, 654
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 1 cut(s) 584
Tru1I TTAA 1 cut(s) 666
Tru9I TTAA 1 cut(s) 666
TscAI CASTG 2 cut(s) 63, 500
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 4 cut(s) 273, 320, 343, 569
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 1 cut(s) 597
TspRI CASTG 2 cut(s) 63, 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 65
XspI CTAG 3 cut(s) 270, 347, 537
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.