Rmu_sc0002250.1_g000021

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002250.1
OG0003915
Physical Location & Seq
Reverse (-)
94218 .. 95376
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 513 bp
atgactaaacctcatttccaagcagtgaaagatgaacagacagtcctgccaaagactggaggatcagcgggctatactatcaaaccacaaggacttaacatagtatggggcaatgatgaacgctactggaaaatacctgccaaaggcaccaatgaggctgcagagctggtgcaggtttcatggcttgaagtaacaggctcagttggtttaatagctgggaagaagtataaactgaactttgaggtagaactgctaccagaggcatttggctggaaagatatacaagctttcttgatggccaaggtagggaaaaagggaaggtacaactggaaaagggttcaactgcagccggggacggaaaaaaccttgattcctgagggagatacgctcaccattgaagttccaactgggcaaaatgtcgacaccacccttcagttcggtctgtatgaagtgtggagtgggaaatggaaaggaggcttgaagatttatgatgcgcaagtaactgagatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

170

Amino Acids

18.99

Weight (kDa)

8.65

Isoelectric Point (pI)

15.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 25 - 163 7e-20 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 495
Acc36I ACCTGC 2 cut(s) 145, 163
AccB1I GGYRCC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 56
AccI GTMKAC 1 cut(s) 420
AciI CCGC 1 cut(s) 68
AclWI GGATC 1 cut(s) 70
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 416
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 3 cut(s) 56, 143, 306
AgsI TTSAA 4 cut(s) 188, 341, 398, 481
AloI GAACNNNNNNTCC 2 cut(s) 27, 59
AluBI AGCT 3 cut(s) 166, 215, 287
AluI AGCT 3 cut(s) 166, 215, 287
AlwI GGATC 1 cut(s) 70
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 2 cut(s) 158, 346
AspLEI GCGC 1 cut(s) 496
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 375
BalI TGGCCA 1 cut(s) 299
BanI GGYRCC 1 cut(s) 146
BbvI GCAGC 2 cut(s) 145, 358
BccI CCATC 1 cut(s) 289
BcnI CCSGG 1 cut(s) 351
BfmI CTRYAG 2 cut(s) 159, 344
BfuAI ACCTGC 2 cut(s) 145, 163
BisI GCNGC 2 cut(s) 159, 347
BlsI GCNGC 2 cut(s) 160, 348
Bme1390I CCNGG 1 cut(s) 351
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 351
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 1 cut(s) 481
BmuI ACTGGG 1 cut(s) 417
BplI GAGNNNNNCTC 2 cut(s) 372, 404
BpmI CTGGAG 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 351
BsaJI CCNNGG 2 cut(s) 300, 350
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 143, 306
Bse1I ACTGG 4 cut(s) 61, 131, 332, 412
Bse21I CCTNAGG 1 cut(s) 375
Bse3DI GCAATG 1 cut(s) 118
BseDI CCNNGG 2 cut(s) 300, 350
BseLI CCNNNNNNNGG 3 cut(s) 56, 143, 306
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 3 cut(s) 213, 366, 495
BseNI ACTGG 4 cut(s) 61, 131, 332, 412
BseXI GCAGC 2 cut(s) 145, 358
BseYI CCCAGC 1 cut(s) 215
BsgI GTGCAG 1 cut(s) 191
BshFI GGCC 1 cut(s) 299
BshNI GGYRCC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 350
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 3 cut(s) 56, 143, 306
BsmFI GGGAC 1 cut(s) 367
BsnI GGCC 1 cut(s) 299
Bsp143I GATC 1 cut(s) 62
BspACI CCGC 1 cut(s) 68
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 3 cut(s) 212, 367, 496
BspLI GGNNCC 1 cut(s) 148
BspMAI CTGCAG 2 cut(s) 163, 348
BspMI ACCTGC 2 cut(s) 145, 163
BspPI GGATC 1 cut(s) 70
BspT107I GGYRCC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 118
BsrI ACTGG 4 cut(s) 61, 131, 332, 412
BssECI CCNNGG 2 cut(s) 300, 350
BssMI GATC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 3 cut(s) 199, 375, 504
BstENI CCTNNNNNAGG 1 cut(s) 141
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 1 cut(s) 65
BstMBI GATC 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 349
BstSFI CTRYAG 2 cut(s) 159, 344
BstV1I GCAGC 2 cut(s) 145, 358
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 1 cut(s) 299
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 30
BveI ACCTGC 2 cut(s) 145, 163
Cac8I GCNNGC 1 cut(s) 70
CfoI GCGC 1 cut(s) 496
Csp6I GTAC 1 cut(s) 322
CviAII CATG 1 cut(s) 180
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 3 cut(s) 199, 375, 504
DpnI GATC 1 cut(s) 64
DpnII GATC 1 cut(s) 62
EaeI YGGCCR 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 300
Eco57I CTGAAG 1 cut(s) 416
Eco81I CCTNAGG 1 cut(s) 375
EcoNI CCTNNNNNAGG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 1 cut(s) 183
FaiI YATR 8 cut(s) 75, 101, 106, 181, 228, 281, 447, 489
FaqI GGGAC 1 cut(s) 367
FatI CATG 1 cut(s) 179
FauI CCCGC 1 cut(s) 61
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 159, 347
Fsp4HI GCNGC 2 cut(s) 159, 347
FspI TGCGCA 1 cut(s) 495
GlaI GCGC 1 cut(s) 495
GluI GCNGC 2 cut(s) 159, 347
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 78
HaeIII GGCC 1 cut(s) 299
HapII CCGG 1 cut(s) 350
HhaI GCGC 1 cut(s) 496
Hin1II CATG 1 cut(s) 183
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 1 cut(s) 370
HpaII CCGG 1 cut(s) 350
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 1 cut(s) 421
Hpy188III TCNNGA 2 cut(s) 292, 374
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 2 cut(s) 312, 440
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4V TGCA 3 cut(s) 161, 172, 346
HpyF3I CTNAG 3 cut(s) 199, 375, 504
Hsp92II CATG 1 cut(s) 183
HspAI GCGC 1 cut(s) 494
Kzo9I GATC 1 cut(s) 62
Lsp1109I GCAGC 2 cut(s) 145, 358
LweI GCATC 1 cut(s) 481
MaeIII GTNAC 2 cut(s) 190, 499
MalI GATC 1 cut(s) 64
MboI GATC 1 cut(s) 62
MboII GAAGA 2 cut(s) 232, 493
MlsI TGGCCA 1 cut(s) 299
MluNI TGGCCA 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 428
MnlI CCTC 7 cut(s) 21, 53, 148, 235, 253, 370, 467
Mox20I TGGCCA 1 cut(s) 299
MscI TGGCCA 1 cut(s) 299
MseI TTAA 3 cut(s) 96, 209, 511
Msp20I TGGCCA 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 1 cut(s) 351
NciI CCSGG 1 cut(s) 351
NdeII GATC 1 cut(s) 62
NlaIII CATG 1 cut(s) 183
NlaIV GGNNCC 1 cut(s) 148
NsbI TGCGCA 1 cut(s) 495
PfeI GAWTC 1 cut(s) 370
PflMI CCANNNNNTGG 1 cut(s) 56
PkrI GCNGC 2 cut(s) 160, 348
PspFI CCCAGC 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 148
PstI CTGCAG 2 cut(s) 163, 348
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 3 cut(s) 96, 209, 511
SatI GCNGC 2 cut(s) 159, 347
Sau3AI GATC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 351
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 2 cut(s) 159, 344
SsiI CCGC 1 cut(s) 68
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 420
TaqII GACCGA 1 cut(s) 428
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 3 cut(s) 96, 209, 511
Tru9I TTAA 3 cut(s) 96, 209, 511
TscAI CASTG 1 cut(s) 30
TseI GCWGC 2 cut(s) 158, 346
TspDTI ATGAA 4 cut(s) 48, 132, 168, 462
TspGWI ACGGA 1 cut(s) 371
TspRI CASTG 1 cut(s) 30
Van91I CCANNNNNTGG 1 cut(s) 56
XagI CCTNNNNNAGG 1 cut(s) 141
XmiI GTMKAC 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.