Rmu_sc0002661.1_g000007

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002661.1
OG0002438
Physical Location & Seq
Forward (+)
32838 .. 66157
33320 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 7884 bp
atggctagggtttacgacaattgggagcggctggtgcgagccacgctcaagagagagcagctcagaacttccggccaaggccatgagagggctccgagtggaatcgccagcgcggttccgtcgtcgctgggcaaggtctcgaatatcgatttgatcttgcaggctgccgatgaaattcaagccgaggatgcaaacgtctccagaattctgtgtgagcaggcgtatagtatggcgcaaaatcttgaccctactagtgatggaagaggagttcttcagtttaagactggtttgatgtctgtaattaagcaaaaacttgcgaaaagggatgggggtcaaatagatcgcaatcgtgatattgagtacctctgggaattttaccagcgctacaagcgacgacatcacctggaggatatccagagggaggaggagagaatgcgtgaatctggcacttttagtgctaactttggaggctataacgagatgaaaaagacaattgctatcctgagggcattgattgaggtgatggagttgctcagcaaagatgcagatcctcatggagttggaaggcaaattagagaggagttgcgcagaattaaaagcactgataccgcattatctagggagctcacagcctacaatattgtcccactgggagcaccatcaataaccaatgcaattggagtatttccagaaattagaggtgcaatgtcggcaattagatatactgaacaattccctaggctgccagctaaagttgagatatctggacaacgggatgcggacatgtttgatcttctggaatttgtgttcggttttcagaaagacaatgtaaggaaccagagagagaatgttgtccttacaattgcaaatgcacaatctcgccttggtatacctgctcaggctgatccaaagatagacgagaaagcaatcaatgaggtatttctgaaagtcctggataattatatcaaatggtgcaaatacctgcggaagcggcttgtatggaatagtttacaagcaattaatcgggacagaaaactttttctggtgtcattgtactttctgatatggggtgaggctgcaaatgtccgctttcttcctgaatgcatttgctacatatttcaccatatggcgaaagagttggatgcaattttagatcatggagaagctattcctgctcctagctgcacaataggaaatgattctgtgtctttccttgatcagattgtttatcctatatatgaaacattggcagcggaagctgctagaaacaataatgggaaagctgcacattcagcatggcggaattatgacgacttcaatgaatacttttggtcacctgcttgttttgacttggactggcctatgaggagggattccacatttttgttgaagcctaaaaagtggaaaaggacaggaaagagcactttcgtcgagcatcgaacatttcttcacttgtatcgaagttttcatcgtctctggatctttctagctttgatgttccagggtttggcaattatagcctttaatgatgggaagatcaattcggatacagtgaagagtgtactcagcattgggccggcatttgctatcatgaacttcgtagaaagtagtttggatgtgttgctcatgtttggggcttataccacagccagaggcatggctatctcaagacttgtaattagatttttctggtttggcttgagttctgtcgctgtgacatatatttatgtgaaggttctgcaggaaagaaatgataaatcaaatgattcattctactttcggatatacattcttgtactgggtgtttatgcagctttacgcctagtccttgcgctattactcaaatttccggcatgtcataagctttctgaaatgtcagatcaatcatttttccaatttttcaagtggatatatgaggagcgttattttgttggacgtggtctttatgaggggatgagcgattatctcaggtctgtgctcttttgggtggtaatcttcacatgcaagttccttttcacgtactttctccagattaagcctcttgttgaaccaacgagaattattgtgaaacttccagctgtgcaatatgcatggcatgatttggtctcaaagaacaacaaaaatgtgttaactatagctagcctatgggctcctgttgtggctatctatctcatggacattcacatatggtacactcttttgtcggccataattggcggtgtaatgggtgcaaggtctcggttaggcgagatacgctcaattgaaatggtgcataaacgttttgagagttttccggaagcttttgtgaagaatctagtttcacaatcacagaaacaaaggtttccgtctaacagtgaaccatctcaggattctcaagtcttaaacaagacaaatgcaacaattttttctccattttggaatgagataataaaaagcttacgagaggaggattttatcagtaatagggagaaggatttactttctattcctagcaacactggaagtctaagattagtgcagtggcctctgtttcttctaagtagtaagatcttgcttgcaattgatttggccttggactgcaaagatacgcaggcagatctttggagcagagtatgcagggatgagtacatggcatatgctgttcaggaatgctactacagtattgaaaaaatattgtattctctggttgagggtgaagggagactttgggtggagaggatctatcgagagatcaacaacagtattatggagggttcgcttgttgtaactttaaatcttgcaaagcttccagcagtgctacgaaaatttacggctttaactgggctgctgatacgtccagaaaccgatgtgcttgcaaaaggtgccgctaaagctgtatttgatgtatatgaggccgttacacatgaccttctgtcacctgatttgagggagcagctcgacacatggagccttttagcaaaagcaagaaatgaagggcgcttgttttctaggataaagtggcccagtgacccagaaactaaagacttggtgaagcgactataccttcttcttactgtaaaggattctgcagcaaatattccgaagaatcttgaagcaagaaggagattagaatttttcacaaattcgttatttatggacatcccttcagcaaagccagttagtgaaatggtacctttcagtgtattcaccccatactacagtgagactgtactttatagttcctccgaattgcgggttgaaaatgaggatggaatttctgttcttttttatcttcagaaaatttttccagatgagtgggacaatttcttggaacgaattggtcaggaccagtccactggggatgctgagcttcaggaaaattcaagtgatgctttggagcttcggttttgggtatcttatcgaggacagactttggccaggactgtgcggggtatgatgtattatagaaaggctttaatgttacagagttatcttgaaagaaggtcattgggagtggatgattattctcaagttgatgcattctcaagtcaaggctttgagtcatcacttgaatcacgagcccaggcagatttgaagtttacttatgttgtatcctgccaaatctatgggcaacagaagcagagaagggcacctgaggctgctgatatatcgctcttgttgcagaggaatgaggcactgcgtgttgcatatatacatgtggaggaaactgctaccgctgaaggacagatcttgaaagaattttactcaaagcttgtaaaagctgatatgcgtggaacggatcaggagatatattccattaaacttcctggtgatccaaagcttggtgaagggaagcctgaaaatcaaaaccatgctatagtcttcactcgcggggaagctgttcaaactattgacatgaaccaggacaattaccttgaggaggcaatgaaaatgagaaatcttctcgaggagtttcacaaaaatcacggtcttcgccccccatctattcttggtgtgagagagcatgtatttactggaagtgtctcatctttagcatggttcatgtccaatcaagagactagctttgtaactttagctcaacgtgttttagcattccctctcaaagttcgtatgcattatgggcatcctgatgtgtttgaccgcatctttcacattactcgaggtggaattagtaaagcttcacgtgttattaacatcagtgaagatatttttgcagggttcaactccacattaaggcagggtaacattacccaccacgaatacattcaggttgggaaaggaagggatgttggcctgaaccagattgcattgtttgaagggaaagttgcaggtggaaatggggagcaagtccttagtagggatgtctacaggcttgggcaacttcttgattttttcagaatgttctctttcttctacacaacagttggttactatgtgtgtacaatgatgaccgtcctcattgtatacatcttcctgtatggaagggcgtacctggctttctctggacttgatcgtgcaattgcgaaggaagcaaaaatatcgggtaacacagcattagatgctgttcttaatgcacagtttttggtgcagattggaattttcacagcagtcccaatgataatgggttttatacttgaattgggattactaaaggcggttttcagttttatcactatgcagctgcagttgtgttctgtgttcttcacattctctttgggaactagaactcattattttggacgtaccatcctccatggaggtgcaaaataccgagcaactggtagggggtttgttgtccgacacataaagtttgctgataattataggctctactctagaagccatttcgtcaaagcgcttgaagtggcccttctcctcatagtttacattgcatatgggtacgcggatggtggtgctgtttcttatattctgctaactcttagcagttggttcctcgtcatctcctggctgtttgctccctacatctttaatccttccggttttgagtggcaaaagacagtggaagattttgatgattggacaagttggcttctttacaaaggtggagtaggagttaagggagaaaatagctgggaatcttggtgggatgaggagcagatgcatattcaaacattgagaggtcgtatcttggaaacaattttgagcttgagattcttcttatttcaatatggcattgtttacaaactccatctcactgggaaggacacatcactcgcgatatacggcttctcttgggttgtgttgataactgttgtcatgatattcaaggtgttcaccttgagtcagaaaaagtcggccaaattccagctgttcatgagatttacacagggtgtctcgtccatgggactggttgcagctattgttctacttgttatattcacgaggctttcaatagcagacctgtttgcaagcatacttgcaatcattccgaccggatgggccataatatgtctagccatcacgtggaagagcattgtcaggagtcttggtttatgggactcggtgagggagtttgccagaatgtacgatgctggcattgatgccttgcatggtaagatatctaatgcttctaccactgcgttggataacctggaactatgtgaatcaagatcttggtttcctctagtcgtgttgcctaggttagggtttcgagtattccaagttgtgcggcgccctagtttcatggctgcagcgattgcttccatgactgccagattggcgaccaagttgtcgctcaaggaaggtgaagaaccggttgacttggggaatctcatggttctgggtaaggagtttttggctcgaaatttctatttggttgggaggcttaatacctgctgggggttggtgctggattcttgctggagtgctgtaaggtcgatgtggaggttgtctggtactgtggaagtccaaccacgaggagaacgttttctatttacgttcacccatgaaagggatgttgctcaagtgaagaagggtggttcgtggggttttcaacgtgccatgattcttctgaataattatgatggcttctcggagatctctgtagtgaagctggactttgtttggatctgggtgacgttggaaggcatacctccgggcattttgactgaacctactgtccggttggttggtgggacgattggcgaggtgctggaggtggatagtacggtgctgcgtcggggagatgctcgggtccagtgtgttctctctatccatgacccgcgttgccctagggtggaggactcggagactgattatgtggtggaggcgggctcgcagccggcaatgggaccgatcgttccagccctagtctttagggaaaacgttcaaccctctctttcttctccgctgctctcttccttcaaggttccaatgctgaagaggaaagctgtgcagatccgcaatctgccagtggttgagtctcccgagactgaagatacttcgactcaaacttcggtggtggggatgcgtcgtcccagagatgaggacggccctgaagaaggtttggttggtgggaagtggtctccagcttcgaaggtgtacaagaagaagggctggcctcgtggctccagagacaaggccaaagcgacaccgccggcgtcctccggtgacggttttgagtatatggcgtcggggtctcctgacgggtgtgacgtgtcttgcttgatggcggcgatgtacaccagaagggtcttaggcatcaatgcggataaaggtcggcccttacctaaatttggtgagtgggatgtaaataacccagcatcagctgaaggatttactgtcatattcaacaaggcccgagatgataagaagaccaatgcgtctcatcctaatattgtgaccccacgaaaaggcgatagcagcgtacgaagagaagaagtggttttgctgtggttgaatccacacactgctttggagcctggtgataatgcaagaggagctacaaggacactgctttcttgtgcctgcgacacattcatattcagagctctcttctcacggccttcatccgcgacgattctctctctcctcacagccttcatcggtgatgagcatggcggcggagacggctctgatgtcagttccggcgacgaggtcgaaaaatcctctctctctctctctctctctctctctctctcagccttcatctgcgaccagttctccgtcgttggccgggttcggcctgaggatccggtgcgccaatcagagcttcctctaactcggtttgctatctcagcgaaccagatgaaggatatggagaagaaccagaactgcgtcatcggcgagtcaaaggtgaatgatccgaacgccgtccacatggagatcgagaatttgatggatgaagatgatggcagtagtactcttcaattcatcaatttctctaggcgtttcatgactaaacgcattgctatacttggaatctatttgaatctcactgtccagaaatcagaatgctcttatggacatgtaatcggagtaattggttctccgctgaaaaggggtggatttgggtctgacatgcagtttgggtctgctttgttgtctctttgtggcttggacagttgtcgacacaacttgaaggaacggcacgaggctgggatctggtttcaggcgacagcaactgcggtggcagcatcgatggggttctggtggtgtttttcggggcgctgggattcatttccactggatcaggctaggtctgttggcgctcgtctgaggttcacctttgttctcggagtggtggcggcgcggtggtggtggtgtttctttgaagtgggcagtgaagatttcaatcgcacaattgtgcggtggttgtctgcggtggccgctggtgtgcgccgtttttttgcacagagaggatga

Protein Analysis

2627

Amino Acids

297.51

Weight (kDa)

8.58

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKS1_dom1 PF14288 345 - 453 5.3e-40 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 842 - 1019 1.4e-46 Callose synthase 1 domain
Glucan_synthase PF02364 1026 - 1804 9.5e-230 1,3-beta-glucan synthase component
AvrRpt-cleavage PF05627 2245 - 2278 2e-17 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1345, 4319
AasI GACNNNNNNGTC 2 cut(s) 5749, 6841
Acc16I TGCGCA 1 cut(s) 587
Acc36I ACCTGC 5 cut(s) 901, 990, 1345, 4319, 5861
Acc65I GGTACC 1 cut(s) 3169
AccB1I GGYRCC 4 cut(s) 2861, 3169, 3628, 5691
AccB7I CCANNNNNTGG 3 cut(s) 1507, 3821, 5484
AccBSI CCGCTC 1 cut(s) 28
AccI GTMKAC 4 cut(s) 889, 4365, 4464, 7588
AccII CGCG 7 cut(s) 113, 3870, 4904, 5237, 6244, 7034, 7771
AccIII TCCGGA 1 cut(s) 2293
AclI AACGTT 3 cut(s) 2278, 5944, 6345
AcoI YGGCCR 6 cut(s) 73, 2205, 3414, 5316, 7192, 7845
AcuI CTGAAG 9 cut(s) 257, 3129, 3257, 3335, 3738, 6419, 6474, 6537, 6810
AcvI CACGTG 2 cut(s) 4184, 5484
AcyI GRCGYC 3 cut(s) 5692, 6620, 6650
AdeI CACNNNGTG 2 cut(s) 3680, 5351
AfeI AGCGCT 2 cut(s) 383, 4857
AflIII ACRYGT 6 cut(s) 783, 3694, 4081, 4183, 6675, 7486
AgeI ACCGGT 1 cut(s) 5773
AhdI GACNNNNNGTC 1 cut(s) 2911
AhlI ACTAGT 1 cut(s) 251
AjiI CACGTC 2 cut(s) 1937, 6676
AjuI GAANNNNNNNTTGG 4 cut(s) 6513, 6545, 7215, 7247
AleI CACNNNNGTG 1 cut(s) 7823
Alw21I GWGCWC 5 cut(s) 627, 658, 1424, 1980, 7012
AlwNI CAGNNNCTG 1 cut(s) 7643
Ama87I CYCGRG 5 cut(s) 3944, 4158, 6210, 6446, 6819
Aor13HI TCCGGA 1 cut(s) 2293
Aor51HI AGCGCT 2 cut(s) 383, 4857
ArsI GACNNNNNNTTYG 4 cut(s) 6457, 6489, 7529, 7561
AseI ATTAAT 1 cut(s) 1020
AsiGI ACCGGT 1 cut(s) 5773
Asp700I GAANNNNTTC 6 cut(s) 1167, 1198, 3879, 4263, 5946, 6345
Asp718I GGTACC 1 cut(s) 3169
AspA2I CCTAGG 3 cut(s) 737, 5657, 6251
AsuC2I CCSGG 2 cut(s) 6117, 7196
AsuII TTCGAA 1 cut(s) 6554
AsuNHI GCTAGC 1 cut(s) 2138
AvaI CYCGRG 5 cut(s) 3944, 4158, 6210, 6446, 6819
AvaII GGWCC 3 cut(s) 3325, 6214, 6311
AvrII CCTAGG 3 cut(s) 737, 5657, 6251
AxyI CCTNAGG 3 cut(s) 503, 3633, 7206
BaeGI GKGCMC 1 cut(s) 3631
BalI TGGCCA 1 cut(s) 3416
BamHI GGATCC 1 cut(s) 7210
BanI GGYRCC 4 cut(s) 2861, 3169, 3628, 5691
BanII GRGCYC 6 cut(s) 94, 627, 2152, 3562, 6296, 7012
BarI GAAGNNNNNNTAC 2 cut(s) 3023, 3055
BauI CACGAG 5 cut(s) 3555, 5401, 5934, 6582, 7610
BbrPI CACGTG 2 cut(s) 4184, 5484
BbsI GAAGAC 3 cut(s) 3853, 3962, 6839
Bbv12I GWGCWC 5 cut(s) 627, 658, 1424, 1980, 7012
BceAI ACGGC 9 cut(s) 2826, 2879, 5260, 6526, 7037, 7105, 7316, 7622, 7845
BcgI CGANNNNNNTGC 6 cut(s) 1411, 1445, 2834, 2868, 4521, 4555
BciVI GTATCC 2 cut(s) 1540, 3601
BclI TGATCA 1 cut(s) 1216
BcnI CCSGG 2 cut(s) 6117, 7196
BcuI ACTAGT 1 cut(s) 251
BfoI RGCGCY 6 cut(s) 385, 2980, 4859, 5695, 7690, 7731
BfuAI ACCTGC 5 cut(s) 901, 990, 1345, 4319, 5861
BfuI GTATCC 2 cut(s) 1540, 3601
BglI GCCNNNNNGGC 1 cut(s) 5738
BglII AGATCT 5 cut(s) 2547, 2596, 3726, 5630, 6057
BlnI CCTAGG 3 cut(s) 737, 5657, 6251
BlpI GCTNAGC 2 cut(s) 533, 3345
BmcAI AGTACT 1 cut(s) 7381
Bme18I GGWCC 3 cut(s) 3325, 6214, 6311
BmeRI GACNNNNNGTC 1 cut(s) 2911
BmeT110I CYCGRG 5 cut(s) 3944, 4158, 6210, 6446, 6819
BmgBI CACGTC 2 cut(s) 1937, 6676
BmrI ACTGGG 6 cut(s) 659, 1808, 2829, 2997, 3345, 5226
BmtI GCTAGC 1 cut(s) 2142
BmuI ACTGGG 6 cut(s) 659, 1808, 2829, 2997, 3345, 5226
BoxI GACNNNNGTC 1 cut(s) 7584
BpiI GAAGAC 3 cut(s) 3853, 3962, 6839
BplI GAGNNNNNCTC 8 cut(s) 30, 62, 45, 77, 2240, 2272, 6278, 6310
BpmI CTGGAG 7 cut(s) 184, 425, 2012, 5902, 6194, 6531, 6574
Bpu10I CCTNAGC 1 cut(s) 897
Bpu1102I GCTNAGC 2 cut(s) 533, 3345
Bpu14I TTCGAA 1 cut(s) 6554
BpuMI CCSGG 2 cut(s) 6117, 7196
Bsa29I ATCGAT 2 cut(s) 147, 7658
BsaAI YACGTR 3 cut(s) 2019, 4184, 5484
BsaBI GATNNNNATC 3 cut(s) 345, 546, 7812
BsaHI GRCGYC 3 cut(s) 5692, 6620, 6650
BsaI GGTCTC 5 cut(s) 142, 2110, 2241, 6549, 6663
BsaWI WCCGGW 7 cut(s) 2293, 4997, 5453, 5773, 6141, 6626, 7213
BsaXI ACNNNNNCTCC 8 cut(s) 3368, 3398, 5058, 5088, 5494, 5524, 7166, 7196
Bse118I RCCGGY 4 cut(s) 1576, 5773, 6301, 6616
Bse21I CCTNAGG 3 cut(s) 503, 3633, 7206
Bse3DI GCAATG 5 cut(s) 711, 3930, 4887, 6312, 7425
Bse8I GATNNNNATC 3 cut(s) 345, 546, 7812
BseAI TCCGGA 1 cut(s) 2293
BseCI ATCGAT 2 cut(s) 147, 7658
BseJI GATNNNNATC 3 cut(s) 345, 546, 7812
BseMI GCAATG 5 cut(s) 711, 3930, 4887, 6312, 7425
BseSI GKGCMC 1 cut(s) 3631
BseYI CCCAGC 6 cut(s) 127, 5091, 5856, 6778, 7616, 7689
BsgI GTGCAG 5 cut(s) 1168, 1269, 2537, 4607, 6434
Bsh1236I CGCG 7 cut(s) 113, 3870, 4904, 5237, 6244, 7034, 7771
Bsh1285I CGRYCG 2 cut(s) 5454, 6318
BshNI GGYRCC 4 cut(s) 2861, 3169, 3628, 5691
BshTI ACCGGT 1 cut(s) 5773
BshVI ATCGAT 2 cut(s) 147, 7658
BsiEI CGRYCG 2 cut(s) 5454, 6318
BsiHKAI GWGCWC 5 cut(s) 627, 658, 1424, 1980, 7012
BsiHKCI CYCGRG 5 cut(s) 3944, 4158, 6210, 6446, 6819
BsiWI CGTACG 1 cut(s) 6886
BslFI GGGAC 9 cut(s) 629, 1040, 3311, 4595, 5379, 5531, 6169, 6324, 6480
BsmBI CGTCTC 4 cut(s) 202, 1478, 6849, 7080
BsmFI GGGAC 9 cut(s) 629, 1040, 3311, 4595, 5379, 5531, 6169, 6324, 6480
BsmI GAATGC 6 cut(s) 438, 1106, 2654, 3518, 4091, 7478
Bso31I GGTCTC 5 cut(s) 142, 2110, 2241, 6549, 6663
BsoBI CYCGRG 5 cut(s) 3944, 4158, 6210, 6446, 6819
Bsp119I TTCGAA 1 cut(s) 6554
Bsp13I TCCGGA 1 cut(s) 2293
Bsp1407I TGTACA 3 cut(s) 4439, 6561, 6699
Bsp1720I GCTNAGC 2 cut(s) 533, 3345
Bsp19I CCATGG 2 cut(s) 4753, 5361
Bsp68I TCGCGA 1 cut(s) 5237
BspDI ATCGAT 2 cut(s) 147, 7658
BspEI TCCGGA 1 cut(s) 2293
BspFNI CGCG 7 cut(s) 113, 3870, 4904, 5237, 6244, 7034, 7771
BspHI TCATGA 4 cut(s) 1590, 5277, 5334, 7413
BspMAI CTGCAG 4 cut(s) 1743, 3070, 4686, 5713
BspMI ACCTGC 5 cut(s) 901, 990, 1345, 4319, 5861
BspOI GCTAGC 1 cut(s) 2142
BspQI GCTCTTC 1 cut(s) 5483
BspT104I TTCGAA 1 cut(s) 6554
BspT107I GGYRCC 4 cut(s) 2861, 3169, 3628, 5691
BspTNI GGTCTC 5 cut(s) 142, 2110, 2241, 6549, 6663
BsrBI CCGCTC 1 cut(s) 28
BsrDI GCAATG 5 cut(s) 711, 3930, 4887, 6312, 7425
BsrFI RCCGGY 4 cut(s) 1576, 5773, 6301, 6616
BsrGI TGTACA 3 cut(s) 4439, 6561, 6699
BssAI RCCGGY 4 cut(s) 1576, 5773, 6301, 6616
BssNAI GTATAC 2 cut(s) 890, 4465
BssNI GRCGYC 3 cut(s) 5692, 6620, 6650
BssSI CACGAG 5 cut(s) 3555, 5401, 5934, 6582, 7610
BssT1I CCWWGG 8 cut(s) 76, 737, 883, 2571, 4753, 5361, 5657, 6251
Bst1107I GTATAC 2 cut(s) 890, 4465
Bst2BI CACGAG 5 cut(s) 3555, 5401, 5934, 6582, 7610
Bst6I CTCTTC 8 cut(s) 256, 1550, 5483, 6382, 6395, 6886, 7019, 7389
BstACI GRCGYC 3 cut(s) 5692, 6620, 6650
BstAPI GCANNNNNTGC 4 cut(s) 2613, 2861, 4559, 5717
BstAUI TGTACA 3 cut(s) 4439, 6561, 6699
BstBAI YACGTR 3 cut(s) 2019, 4184, 5484
BstBI TTCGAA 1 cut(s) 6554
BstDSI CCRYGG 2 cut(s) 4753, 5361
BstEII GGTNACC 1 cut(s) 1332
BstENI CCTNNNNNAGG 3 cut(s) 3917, 5661, 6519
BstFNI CGCG 7 cut(s) 113, 3870, 4904, 5237, 6244, 7034, 7771
BstH2I RGCGCY 6 cut(s) 385, 2980, 4859, 5695, 7690, 7731
BstMCI CGRYCG 2 cut(s) 5454, 6318
BstNSI RCATGY 7 cut(s) 787, 1857, 2004, 3698, 4007, 7490, 7543
BstPAI GACNNNNGTC 1 cut(s) 7584
BstPI GGTNACC 1 cut(s) 1332
BstSLI GKGCMC 1 cut(s) 3631
BstUI CGCG 7 cut(s) 113, 3870, 4904, 5237, 6244, 7034, 7771
BstV2I GAAGAC 3 cut(s) 3853, 3962, 6839
BstXI CCANNNNNNTGG 7 cut(s) 1657, 3294, 3335, 3605, 5216, 5368, 5602
BstZ17I GTATAC 2 cut(s) 890, 4465
Bsu15I ATCGAT 2 cut(s) 147, 7658
Bsu36I CCTNAGG 3 cut(s) 503, 3633, 7206
BsuI GTATCC 2 cut(s) 1540, 3601
BsuTUI ATCGAT 2 cut(s) 147, 7658
BtgI CCRYGG 2 cut(s) 4753, 5361
BtgZI GCGATG 1 cut(s) 6710
BtrI CACGTC 2 cut(s) 1937, 6676
BtsI GCAGTG 7 cut(s) 2525, 2799, 3674, 5595, 6927, 6971, 7807
BtuMI TCGCGA 1 cut(s) 5237
BveI ACCTGC 5 cut(s) 901, 990, 1345, 4319, 5861
CaiI CAGNNNCTG 1 cut(s) 7643
CciI TCATGA 4 cut(s) 1590, 5277, 5334, 7413
Cfr10I RCCGGY 4 cut(s) 1576, 5773, 6301, 6616
ClaI ATCGAT 2 cut(s) 147, 7658
CseI GACGC 6 cut(s) 6185, 6479, 6609, 6639, 6831, 7285
CspAI ACCGGT 1 cut(s) 5773
CspCI CAANNNNNGTGG 2 cut(s) 7629, 7664
DinI GGCGCC 1 cut(s) 5693
DraI TTTAAA 1 cut(s) 2772
DraIII CACNNNGTG 2 cut(s) 3680, 5351
DrdI GACNNNNNNGTC 2 cut(s) 5749, 6841
DriI GACNNNNNGTC 1 cut(s) 2911
DseDI GACNNNNNNGTC 2 cut(s) 5749, 6841
EaeI YGGCCR 6 cut(s) 73, 2205, 3414, 5316, 7192, 7845
Eam1104I CTCTTC 8 cut(s) 256, 1550, 5483, 6382, 6395, 6886, 7019, 7389
Eam1105I GACNNNNNGTC 1 cut(s) 2911
EarI CTCTTC 8 cut(s) 256, 1550, 5483, 6382, 6395, 6886, 7019, 7389
EciI GGCGGA 2 cut(s) 1315, 7098
Ecl136II GAGCTC 2 cut(s) 625, 7010
Eco130I CCWWGG 8 cut(s) 76, 737, 883, 2571, 4753, 5361, 5657, 6251
Eco24I GRGCYC 6 cut(s) 94, 627, 2152, 3562, 6296, 7012
Eco31I GGTCTC 5 cut(s) 142, 2110, 2241, 6549, 6663
Eco32I GATATC 3 cut(s) 412, 762, 5580
Eco47I GGWCC 3 cut(s) 3325, 6214, 6311
Eco47III AGCGCT 2 cut(s) 383, 4857
Eco53kI GAGCTC 2 cut(s) 625, 7010
Eco57I CTGAAG 9 cut(s) 257, 3129, 3257, 3335, 3738, 6419, 6474, 6537, 6810
Eco72I CACGTG 2 cut(s) 4184, 5484
Eco81I CCTNAGG 3 cut(s) 503, 3633, 7206
Eco88I CYCGRG 5 cut(s) 3944, 4158, 6210, 6446, 6819
Eco91I GGTNACC 1 cut(s) 1332
EcoICRI GAGCTC 2 cut(s) 625, 7010
EcoNI CCTNNNNNAGG 3 cut(s) 3917, 5661, 6519
EcoO65I GGTNACC 1 cut(s) 1332
EcoRI GAATTC 1 cut(s) 204
EcoRV GATATC 3 cut(s) 412, 762, 5580
EcoT14I CCWWGG 8 cut(s) 76, 737, 883, 2571, 4753, 5361, 5657, 6251
EcoT22I ATGCAT 5 cut(s) 1106, 2092, 3520, 4116, 5124
EcoT38I GRGCYC 6 cut(s) 94, 627, 2152, 3562, 6296, 7012
EgeI GGCGCC 1 cut(s) 5693
EheI GGCGCC 1 cut(s) 5693
ErhI CCWWGG 8 cut(s) 76, 737, 883, 2571, 4753, 5361, 5657, 6251
Esp3I CGTCTC 4 cut(s) 202, 1478, 6849, 7080
FalI AAGNNNNNCTT 8 cut(s) 2688, 2720, 3355, 3387, 4854, 4886, 6062, 6094
FaqI GGGAC 9 cut(s) 629, 1040, 3311, 4595, 5379, 5531, 6169, 6324, 6480
FauI CCCGC 5 cut(s) 3225, 3420, 3863, 6249, 6283
FauNDI CATATG 4 cut(s) 1125, 2186, 2635, 4894
FbaI TGATCA 1 cut(s) 1216
FblI GTMKAC 4 cut(s) 889, 4365, 4464, 7588
FriOI GRGCYC 6 cut(s) 94, 627, 2152, 3562, 6296, 7012
FspI TGCGCA 1 cut(s) 587
GsaI CCCAGC 6 cut(s) 131, 5095, 5860, 6782, 7620, 7693
GsuI CTGGAG 7 cut(s) 184, 425, 2012, 5902, 6194, 6531, 6574
HaeII RGCGCY 6 cut(s) 385, 2980, 4859, 5695, 7690, 7731
HgaI GACGC 6 cut(s) 6185, 6479, 6609, 6639, 6831, 7285
Hin1I GRCGYC 3 cut(s) 5692, 6620, 6650
HincII GTYRAC 3 cut(s) 2130, 5779, 7589
HindII GTYRAC 3 cut(s) 2130, 5779, 7589
HindIII AAGCTT 7 cut(s) 1862, 2298, 2434, 2783, 3749, 3818, 4176
HpaI GTTAAC 1 cut(s) 2130
Hsp92I GRCGYC 3 cut(s) 5692, 6620, 6650
KasI GGCGCC 1 cut(s) 5691
Kpn2I TCCGGA 1 cut(s) 2293
KpnI GGTACC 1 cut(s) 3173
KroI GCCGGC 3 cut(s) 1576, 6301, 6616
KroNI GCCGGC 3 cut(s) 1578, 6303, 6618
Ksp22I TGATCA 1 cut(s) 1216
KspAI GTTAAC 1 cut(s) 2130
LguI GCTCTTC 1 cut(s) 5483
MbiI CCGCTC 1 cut(s) 28
MfeI CAATTG 8 cut(s) 19, 492, 675, 861, 2259, 2559, 4518, 7821
MlsI TGGCCA 1 cut(s) 3416
MluNI TGGCCA 1 cut(s) 3416
Mly113I GGCGCC 1 cut(s) 5692
MlyI GAGTC 8 cut(s) 3548, 5311, 5512, 5513, 6257, 6449, 6460, 7316
MmeI TCCRAC 8 cut(s) 541, 1119, 1912, 4822, 5474, 5583, 5953, 6081
Mox20I TGGCCA 1 cut(s) 3416
Mph1103I ATGCAT 5 cut(s) 1106, 2092, 3520, 4116, 5124
MreI CGCCGGCG 1 cut(s) 6616
MroI TCCGGA 1 cut(s) 2293
MroNI GCCGGC 3 cut(s) 1576, 6301, 6616
MroXI GAANNNNTTC 6 cut(s) 1167, 1198, 3879, 4263, 5946, 6345
MscI TGGCCA 1 cut(s) 3416
MslI CAYNNNNRTG 3 cut(s) 4128, 4758, 7823
Msp20I TGGCCA 1 cut(s) 3416
MspA1I CMGCKG 9 cut(s) 1253, 2078, 3716, 4681, 5329, 6370, 6788, 7513, 7850
MunI CAATTG 8 cut(s) 19, 492, 675, 861, 2259, 2559, 4518, 7821
Mva1269I GAATGC 6 cut(s) 438, 1106, 2654, 3518, 4091, 7478
MvnI CGCG 7 cut(s) 113, 3870, 4904, 5237, 6244, 7034, 7771
NaeI GCCGGC 3 cut(s) 1578, 6303, 6618
NarI GGCGCC 1 cut(s) 5692
NciI CCSGG 2 cut(s) 6117, 7196
NcoI CCATGG 2 cut(s) 4753, 5361
NdeI CATATG 4 cut(s) 1125, 2186, 2635, 4894
NgoMIV GCCGGC 3 cut(s) 1576, 6301, 6616
NheI GCTAGC 1 cut(s) 2138
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 8 cut(s) 1332, 1714, 2913, 3005, 6094, 6629, 6671, 6859
NruI TCGCGA 1 cut(s) 5237
NsbI TGCGCA 1 cut(s) 587
NsiI ATGCAT 5 cut(s) 1106, 2092, 3520, 4116, 5124
NspI RCATGY 7 cut(s) 787, 1857, 2004, 3698, 4007, 7490, 7543
NspV TTCGAA 1 cut(s) 6554
OliI CACNNNNGTG 1 cut(s) 7823
PaeR7I CTCGAG 2 cut(s) 3944, 4158
PagI TCATGA 4 cut(s) 1590, 5277, 5334, 7413
PaqCI CACCTGC 2 cut(s) 1345, 4319
PciI ACATGT 3 cut(s) 783, 3694, 7486
PciSI GCTCTTC 1 cut(s) 5483
PcsI WCGNNNNNNNCGW 2 cut(s) 5941, 6672
PctI GAATGC 6 cut(s) 438, 1106, 2654, 3518, 4091, 7478
PdiI GCCGGC 3 cut(s) 1578, 6303, 6618
PdmI GAANNNNTTC 6 cut(s) 1167, 1198, 3879, 4263, 5946, 6345
Pfl23II CGTACG 1 cut(s) 6886
PflFI GACNNNGTC 2 cut(s) 1938, 7115
PflMI CCANNNNNTGG 3 cut(s) 1507, 3821, 5484
PfoI TCCNGGA 1 cut(s) 951
PinAI ACCGGT 1 cut(s) 5773
Ple19I CGATCG 1 cut(s) 6318
PleI GAGTC 8 cut(s) 3547, 5310, 5511, 5513, 6257, 6448, 6460, 7315
PluTI GGCGCC 1 cut(s) 5695
PmaCI CACGTG 2 cut(s) 4184, 5484
PmlI CACGTG 2 cut(s) 4184, 5484
PpsI GAGTC 8 cut(s) 3547, 5310, 5511, 5513, 6257, 6448, 6460, 7315
Ppu21I YACGTR 3 cut(s) 2019, 4184, 5484
PscI ACATGT 3 cut(s) 783, 3694, 7486
PshAI GACNNNNGTC 1 cut(s) 7584
PshBI ATTAAT 1 cut(s) 1020
Psp124BI GAGCTC 2 cut(s) 627, 7012
Psp1406I AACGTT 3 cut(s) 2278, 5944, 6345
PspCI CACGTG 2 cut(s) 4184, 5484
PspEI GGTNACC 1 cut(s) 1332
PspFI CCCAGC 6 cut(s) 127, 5091, 5856, 6778, 7616, 7689
PspLI CGTACG 1 cut(s) 6886
PspXI VCTCGAGB 1 cut(s) 4158
PstI CTGCAG 4 cut(s) 1743, 3070, 4686, 5713
PstNI CAGNNNCTG 1 cut(s) 7643
PsyI GACNNNGTC 2 cut(s) 1938, 7115
PvuI CGATCG 1 cut(s) 6318
PvuII CAGCTG 4 cut(s) 2078, 4681, 5329, 6788
RruI TCGCGA 1 cut(s) 5237
RseI CAYNNNNRTG 3 cut(s) 4128, 4758, 7823
SacI GAGCTC 2 cut(s) 627, 7012
SalI GTCGAC 1 cut(s) 7587
SapI GCTCTTC 1 cut(s) 5483
ScaI AGTACT 1 cut(s) 7381
SchI GAGTC 8 cut(s) 3548, 5311, 5512, 5513, 6257, 6449, 6460, 7316
SfoI GGCGCC 1 cut(s) 5693
Sfr274I CTCGAG 2 cut(s) 3944, 4158
SfuI TTCGAA 1 cut(s) 6554
SgrAI CRCCGGYG 1 cut(s) 6616
SinI GGWCC 3 cut(s) 3325, 6214, 6311
SlaI CTCGAG 2 cut(s) 3944, 4158
SmiMI CAYNNNNRTG 3 cut(s) 4128, 4758, 7823
SpeI ACTAGT 1 cut(s) 251
SspDI GGCGCC 1 cut(s) 5691
SspI AATATT 4 cut(s) 640, 2673, 3076, 6856
SstI GAGCTC 2 cut(s) 627, 7012
StyI CCWWGG 8 cut(s) 76, 737, 883, 2571, 4753, 5361, 5657, 6251
TaqII GACCGA 1 cut(s) 6328
TatI WGTACW 9 cut(s) 1053, 1561, 1795, 2625, 3208, 4439, 6561, 6699, 7379
TauI GCSGC 8 cut(s) 31, 994, 2867, 5692, 6695, 7083, 7769, 7850
TseFI GTSAC 8 cut(s) 1332, 1714, 2913, 3005, 6094, 6629, 6671, 6859
Tsp45I GTSAC 8 cut(s) 1332, 1714, 2913, 3005, 6094, 6629, 6671, 6859
TspGWI ACGGA 4 cut(s) 108, 2334, 3791, 7174
Tth111I GACNNNGTC 2 cut(s) 1938, 7115
Van91I CCANNNNNTGG 3 cut(s) 1507, 3821, 5484
VpaK11BI GGWCC 3 cut(s) 3325, 6214, 6311
VspI ATTAAT 1 cut(s) 1020
XagI CCTNNNNNAGG 3 cut(s) 3917, 5661, 6519
XbaI TCTAGA 1 cut(s) 4835
XceI RCATGY 7 cut(s) 787, 1857, 2004, 3698, 4007, 7490, 7543
XhoI CTCGAG 2 cut(s) 3944, 4158
XmaJI CCTAGG 3 cut(s) 737, 5657, 6251
XmiI GTMKAC 4 cut(s) 889, 4365, 4464, 7588
XmnI GAANNNNTTC 6 cut(s) 1167, 1198, 3879, 4263, 5946, 6345
ZrmI AGTACT 1 cut(s) 7381
Zsp2I ATGCAT 5 cut(s) 1106, 2092, 3520, 4116, 5124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.