Rmu_sc0003168.1_g000006

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003168.1
OG0003654
Physical Location & Seq
Reverse (-)
34450 .. 35766
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1317 bp
atggcgcgtagggctctagttagaatgattgtcataattttgtatgctttcgtcactatttctcaagcggaaaatataataacagttgatcaatccggtaacggtcattacggtaaaatacaagatgctattgattctgttccgtcaaacaataaggatcctttgactatttcgatcaagcctgggacttacaaagaaagtattaccatacctccagacaagccattcataacattaagtggtactactacagaagcaagtgatgtgatgataacgtgggatcagagaggggacattgataaagttacaacatttcatgtttccgcttccgactttgtagctcgctacataacaattcaaaatacattcgggcctggtcatcaagcagttgcgctaagggtctccggcgatagaatagcgttttatgactgctcaattttaggttatcaggatactctatttgacgaaataggcaaacactactatgaaaattgttacattgaaggagttgttgattttatatttggaagcgccagttctttctttcaaaattgtcacatacattcgcctacaggaggaggatatattacagcgcaacgaagagaagcgtcaacagatagtggagggtttgtgttctttaagtgtcaaattactggcaaaggcgaaaaaacatttttaggaagaccatgggtgggatcctatgctagggtcatatttgcctacactaacatgcaagatgtgatagaaccactaggttggtctcgttattctgatggatccacagaacaagaattaatgacaaactcattttgtggacaattcaaatgcgacggacttggagcaacaacatccaacagggttaaatgggcgcgagaattcagtgagaaacaaattgcaaattttttgacaacagactttatcaatggaaaggattggataaagtctacgccggtaacaacgtcgccaccacctaaaaaggagacgccgtcattgcctccatcggtactggatacaccgccaccctcaccaccacctcccatgaagccgtctgcaccaccggtcatcccggcacctgtaatcccgtctccaccaccacaagccccggtgattccattagtttccccaccagcaccggcagtgattataactccgtggtcgcctccaccaacatacatggacggcagagctcaagaaggtgaacctatcatcatgtattattattcctcgggggcatcctcttcaatctttaaaatttgtttcagcggcatcgggacgctggctcttacctcggcagttttactatacttgtcggcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

438

Amino Acids

47.75

Weight (kDa)

5.74

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 27 - 309 3.5e-64 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1145
AccB1I GGYRCC 1 cut(s) 1069
AccI GTMKAC 1 cut(s) 944
AccII CGCG 2 cut(s) 7, 871
AciI CCGC 4 cut(s) 68, 324, 1016, 1263
AclWI GGATC 7 cut(s) 152, 165, 288, 690, 703, 771, 784
AcsI RAATTY 3 cut(s) 875, 898, 1251
AcyI GRCGYC 1 cut(s) 983
AfaI GTAC 2 cut(s) 244, 1005
AfiI CCNNNNNNNGG 3 cut(s) 575, 692, 705
AgeI ACCGGT 1 cut(s) 1057
AgsI TTSAA 5 cut(s) 359, 503, 548, 823, 1242
AjnI CCWGG 2 cut(s) 181, 373
AluBI AGCT 2 cut(s) 341, 1187
AluI AGCT 2 cut(s) 341, 1187
Alw21I GWGCWC 1 cut(s) 1189
Alw26I GTCTC 4 cut(s) 406, 765, 974, 1089
AlwI GGATC 7 cut(s) 152, 165, 288, 690, 703, 771, 784
Ama87I CYCGRG 1 cut(s) 1225
AoxI GGCC 2 cut(s) 371, 1311
ApoI RAATTY 3 cut(s) 875, 898, 1251
AseI ATTAAT 1 cut(s) 794
AsiGI ACCGGT 1 cut(s) 1057
AspLEI GCGC 5 cut(s) 7, 394, 533, 595, 871
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 2 cut(s) 1067, 1103
AsuHPI GGTGA 3 cut(s) 1017, 1117, 1208
AvaI CYCGRG 1 cut(s) 1225
BamHI GGATCC 3 cut(s) 157, 695, 776
BanI GGYRCC 1 cut(s) 1069
BanII GRGCYC 2 cut(s) 16, 1189
BbsI GAAGAC 1 cut(s) 688
Bbv12I GWGCWC 1 cut(s) 1189
BccI CCATC 2 cut(s) 767, 1006
BceAI ACGGC 3 cut(s) 970, 1030, 1195
BciT130I CCWGG 2 cut(s) 183, 375
BciVI GTATCC 2 cut(s) 445, 1003
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 2 cut(s) 1067, 1103
BcoDI GTCTC 4 cut(s) 406, 765, 974, 1089
BfaI CTAG 3 cut(s) 17, 705, 752
BfmI CTRYAG 2 cut(s) 249, 570
BfoI RGCGCY 1 cut(s) 534
BfuI GTATCC 2 cut(s) 445, 1003
BisI GCNGC 1 cut(s) 1264
BlsI GCNGC 1 cut(s) 1265
Bme1390I CCNGG 4 cut(s) 183, 375, 1067, 1103
BmeT110I CYCGRG 1 cut(s) 1225
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 4 cut(s) 159, 697, 778, 1071
BmrFI CCNGG 4 cut(s) 183, 375, 1067, 1103
BmsI GCATC 3 cut(s) 115, 1241, 1275
BpiI GAAGAC 1 cut(s) 688
BpmI CTGGAG 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 48, 1173
BpuMI CCSGG 2 cut(s) 1067, 1103
BsaHI GRCGYC 1 cut(s) 983
BsaI GGTCTC 2 cut(s) 406, 765
BsaJI CCNNGG 6 cut(s) 182, 686, 1101, 1151, 1224, 1287
BsaWI WCCGGW 2 cut(s) 95, 1057
BsaXI ACNNNNNCTCC 6 cut(s) 196, 226, 615, 645, 971, 1001
Bsc4I CCNNNNNNNGG 3 cut(s) 575, 692, 705
Bse118I RCCGGY 3 cut(s) 949, 1057, 1132
Bse1I ACTGG 3 cut(s) 534, 658, 1011
Bse3DI GCAATG 1 cut(s) 989
BseBI CCWGG 2 cut(s) 183, 375
BseDI CCNNGG 6 cut(s) 182, 686, 1101, 1151, 1224, 1287
BseGI GGATG 3 cut(s) 848, 1062, 1232
BseLI CCNNNNNNNGG 3 cut(s) 575, 692, 705
BseMI GCAATG 1 cut(s) 989
BseNI ACTGG 3 cut(s) 534, 658, 1011
BseRI GAGGAG 1 cut(s) 591
BsgI GTGCAG 1 cut(s) 1035
Bsh1236I CGCG 2 cut(s) 7, 871
BshFI GGCC 2 cut(s) 373, 1313
BshNI GGYRCC 1 cut(s) 1069
BshTI ACCGGT 1 cut(s) 1057
BsiHKAI GWGCWC 1 cut(s) 1189
BsiHKCI CYCGRG 1 cut(s) 1225
BsiSI CCGG 7 cut(s) 96, 405, 950, 1058, 1067, 1103, 1133
BslFI GGGAC 3 cut(s) 199, 305, 1285
BslI CCNNNNNNNGG 3 cut(s) 575, 692, 705
BsmAI GTCTC 4 cut(s) 406, 765, 974, 1089
BsmBI CGTCTC 2 cut(s) 974, 1089
BsmFI GGGAC 3 cut(s) 199, 305, 1285
BsnI GGCC 2 cut(s) 373, 1313
Bso31I GGTCTC 2 cut(s) 406, 765
BsoBI CYCGRG 1 cut(s) 1225
Bsp1286I GDGCHC 2 cut(s) 16, 1189
Bsp143I GATC 6 cut(s) 88, 157, 174, 280, 695, 776
Bsp19I CCATGG 1 cut(s) 686
BspACI CCGC 4 cut(s) 68, 324, 1016, 1263
BspANI GGCC 2 cut(s) 373, 1313
BspFNI CGCG 2 cut(s) 7, 871
BspLI GGNNCC 4 cut(s) 159, 697, 778, 1071
BspPI GGATC 7 cut(s) 152, 165, 288, 690, 703, 771, 784
BspT107I GGYRCC 1 cut(s) 1069
BspTNI GGTCTC 2 cut(s) 406, 765
BsrDI GCAATG 1 cut(s) 989
BsrFI RCCGGY 3 cut(s) 949, 1057, 1132
BsrI ACTGG 3 cut(s) 534, 658, 1011
BssAI RCCGGY 3 cut(s) 949, 1057, 1132
BssECI CCNNGG 6 cut(s) 182, 686, 1101, 1151, 1224, 1287
BssMI GATC 6 cut(s) 88, 157, 174, 280, 695, 776
BssNI GRCGYC 1 cut(s) 983
BssT1I CCWWGG 1 cut(s) 686
Bst2UI CCWGG 2 cut(s) 183, 375
Bst4CI ACNGT 3 cut(s) 85, 104, 113
Bst6I CTCTTC 2 cut(s) 595, 1243
BstACI GRCGYC 1 cut(s) 983
BstC8I GCNNGC 2 cut(s) 343, 1278
BstDEI CTNAG 1 cut(s) 395
BstDSI CCRYGG 2 cut(s) 686, 1151
BstENI CCTNNNNNAGG 2 cut(s) 573, 703
BstF5I GGATG 3 cut(s) 848, 1062, 1232
BstFNI CGCG 2 cut(s) 7, 871
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 5 cut(s) 7, 394, 533, 595, 871
BstKTI GATC 6 cut(s) 91, 160, 177, 283, 698, 779
BstMAI GTCTC 4 cut(s) 406, 765, 974, 1089
BstMBI GATC 6 cut(s) 88, 157, 174, 280, 695, 776
BstMWI GCNNNNNNNGC 2 cut(s) 11, 991
BstNI CCWGG 2 cut(s) 183, 375
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 4 cut(s) 181, 373, 1065, 1101
BstSFI CTRYAG 2 cut(s) 249, 570
BstUI CGCG 2 cut(s) 7, 871
BstV2I GAAGAC 1 cut(s) 688
BstX2I RGATCY 3 cut(s) 157, 695, 776
BstXI CCANNNNNNTGG 1 cut(s) 756
BstYI RGATCY 3 cut(s) 157, 695, 776
BsuI GTATCC 2 cut(s) 445, 1003
BsuRI GGCC 2 cut(s) 373, 1313
BtgI CCRYGG 2 cut(s) 686, 1151
BtsCI GGATG 3 cut(s) 848, 1062, 1232
BtsI GCAGTG 1 cut(s) 1143
BtsIMutI CAGTG 2 cut(s) 886, 1143
Cac8I GCNNGC 2 cut(s) 343, 1278
CfoI GCGC 5 cut(s) 7, 394, 533, 595, 871
Cfr10I RCCGGY 3 cut(s) 949, 1057, 1132
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 3 cut(s) 597, 991, 1282
Csp6I GTAC 2 cut(s) 243, 1004
CspAI ACCGGT 1 cut(s) 1057
CviAII CATG 6 cut(s) 317, 687, 730, 1039, 1174, 1210
CviQI GTAC 2 cut(s) 243, 1004
DdeI CTNAG 1 cut(s) 395
DpnI GATC 6 cut(s) 90, 159, 176, 282, 697, 778
DpnII GATC 6 cut(s) 88, 157, 174, 280, 695, 776
DraI TTTAAA 1 cut(s) 1249
Eam1104I CTCTTC 2 cut(s) 595, 1243
EarI CTCTTC 2 cut(s) 595, 1243
Ecl136II GAGCTC 1 cut(s) 1187
Eco130I CCWWGG 1 cut(s) 686
Eco24I GRGCYC 2 cut(s) 16, 1189
Eco31I GGTCTC 2 cut(s) 406, 765
Eco53kI GAGCTC 1 cut(s) 1187
Eco88I CYCGRG 1 cut(s) 1225
EcoICRI GAGCTC 1 cut(s) 1187
EcoNI CCTNNNNNAGG 2 cut(s) 573, 703
EcoRI GAATTC 1 cut(s) 875
EcoRII CCWGG 2 cut(s) 181, 373
EcoT14I CCWWGG 1 cut(s) 686
EcoT38I GRGCYC 2 cut(s) 16, 1189
ErhI CCWWGG 1 cut(s) 686
Esp3I CGTCTC 2 cut(s) 974, 1089
FaeI CATG 6 cut(s) 320, 690, 733, 1042, 1177, 1213
FaqI GGGAC 3 cut(s) 199, 305, 1285
FatI CATG 6 cut(s) 316, 686, 729, 1038, 1173, 1209
FbaI TGATCA 1 cut(s) 88
FblI GTMKAC 1 cut(s) 944
Fnu4HI GCNGC 1 cut(s) 1264
FokI GGATG 3 cut(s) 835, 1049, 1219
FriOI GRGCYC 2 cut(s) 16, 1189
Fsp4HI GCNGC 1 cut(s) 1264
FspBI CTAG 3 cut(s) 17, 705, 752
GlaI GCGC 5 cut(s) 6, 393, 532, 594, 870
GluI GCNGC 1 cut(s) 1264
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 2 cut(s) 373, 1313
HapII CCGG 7 cut(s) 96, 405, 950, 1058, 1067, 1103, 1133
HgaI GACGC 3 cut(s) 597, 991, 1282
HhaI GCGC 5 cut(s) 7, 394, 533, 595, 871
Hin1I GRCGYC 1 cut(s) 983
Hin1II CATG 6 cut(s) 320, 690, 733, 1042, 1177, 1213
Hin6I GCGC 5 cut(s) 5, 392, 531, 593, 869
HinP1I GCGC 5 cut(s) 5, 392, 531, 593, 869
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HinfI GANTC 2 cut(s) 134, 1108
HpaII CCGG 7 cut(s) 96, 405, 950, 1058, 1067, 1103, 1133
HphI GGTGA 3 cut(s) 1017, 1117, 1208
Hpy166II GTNNAC 4 cut(s) 612, 815, 945, 1199
Hpy188I TCNGA 3 cut(s) 285, 331, 772
Hpy188III TCNNGA 4 cut(s) 215, 449, 1190, 1270
Hpy8I GTNNAC 4 cut(s) 612, 815, 945, 1199
Hpy99I CGWCG 2 cut(s) 833, 964
HpyAV CCTTC 2 cut(s) 497, 1187
HpyCH4III ACNGT 3 cut(s) 85, 104, 113
HpyCH4IV ACGT 2 cut(s) 275, 959
HpyCH4V TGCA 3 cut(s) 733, 896, 1052
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 991
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 2 cut(s) 275, 959
Hsp92I GRCGYC 1 cut(s) 983
Hsp92II CATG 6 cut(s) 320, 690, 733, 1042, 1177, 1213
HspAI GCGC 5 cut(s) 5, 392, 531, 593, 869
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 6 cut(s) 88, 157, 174, 280, 695, 776
LmnI GCTCC 1 cut(s) 839
LweI GCATC 3 cut(s) 115, 1241, 1275
MaeI CTAG 3 cut(s) 17, 705, 752
MaeII ACGT 2 cut(s) 275, 959
MaeIII GTNAC 6 cut(s) 52, 98, 304, 494, 554, 952
MalI GATC 6 cut(s) 90, 159, 176, 282, 697, 778
MboI GATC 6 cut(s) 88, 157, 174, 280, 695, 776
MboII GAAGA 3 cut(s) 612, 693, 1230
MflI RGATCY 3 cut(s) 157, 695, 776
MhlI GDGCHC 2 cut(s) 16, 1189
MmeI TCCRAC 2 cut(s) 354, 876
MseI TTAA 5 cut(s) 236, 639, 794, 861, 1248
MslI CAYNNNNRTG 2 cut(s) 483, 728
MspA1I CMGCKG 1 cut(s) 1263
MspI CCGG 7 cut(s) 96, 405, 950, 1058, 1067, 1103, 1133
MspR9I CCNGG 4 cut(s) 183, 375, 1067, 1103
MvaI CCWGG 2 cut(s) 183, 375
MvnI CGCG 2 cut(s) 7, 871
MwoI GCNNNNNNNGC 2 cut(s) 11, 991
NciI CCSGG 2 cut(s) 1067, 1103
NcoI CCATGG 1 cut(s) 686
NdeII GATC 6 cut(s) 88, 157, 174, 280, 695, 776
NlaIII CATG 6 cut(s) 320, 690, 733, 1042, 1177, 1213
NlaIV GGNNCC 4 cut(s) 159, 697, 778, 1071
NmeAIII GCCGAG 1 cut(s) 1268
NmuCI GTSAC 2 cut(s) 52, 554
NspI RCATGY 1 cut(s) 733
PfeI GAWTC 2 cut(s) 134, 1108
PflFI GACNNNGTC 1 cut(s) 985
PinAI ACCGGT 1 cut(s) 1057
PkrI GCNGC 1 cut(s) 1265
PshBI ATTAAT 1 cut(s) 794
PsiI TTATAA 1 cut(s) 1145
Psp124BI GAGCTC 1 cut(s) 1189
Psp6I CCWGG 2 cut(s) 181, 373
PspGI CCWGG 2 cut(s) 181, 373
PspN4I GGNNCC 4 cut(s) 159, 697, 778, 1071
PspPI GGNCC 1 cut(s) 371
PsuI RGATCY 3 cut(s) 157, 695, 776
PsyI GACNNNGTC 1 cut(s) 985
RsaI GTAC 2 cut(s) 244, 1005
RsaNI GTAC 2 cut(s) 243, 1004
RseI CAYNNNNRTG 2 cut(s) 483, 728
SacI GAGCTC 1 cut(s) 1189
SaqAI TTAA 5 cut(s) 236, 639, 794, 861, 1248
SatI GCNGC 1 cut(s) 1264
Sau3AI GATC 6 cut(s) 88, 157, 174, 280, 695, 776
Sau96I GGNCC 1 cut(s) 371
ScrFI CCNGG 4 cut(s) 183, 375, 1067, 1103
SduI GDGCHC 2 cut(s) 16, 1189
SfaNI GCATC 3 cut(s) 115, 1241, 1275
SfcI CTRYAG 2 cut(s) 249, 570
SmiMI CAYNNNNRTG 2 cut(s) 483, 728
SmlI CTYRAG 2 cut(s) 63, 1188
SmoI CTYRAG 2 cut(s) 63, 1188
SsiI CCGC 4 cut(s) 68, 324, 1016, 1263
SspMI CTAG 3 cut(s) 17, 705, 752
SstI GAGCTC 1 cut(s) 1189
StyD4I CCNGG 4 cut(s) 181, 373, 1065, 1101
StyI CCWWGG 1 cut(s) 686
TaaI ACNGT 3 cut(s) 85, 104, 113
TaiI ACGT 2 cut(s) 278, 962
TaqI TCGA 1 cut(s) 173
TauI GCSGC 1 cut(s) 1266
TfiI GAWTC 2 cut(s) 134, 1108
Tru1I TTAA 5 cut(s) 236, 639, 794, 861, 1248
Tru9I TTAA 5 cut(s) 236, 639, 794, 861, 1248
TscAI CASTG 2 cut(s) 886, 1143
TseFI GTSAC 2 cut(s) 52, 554
Tsp45I GTSAC 2 cut(s) 52, 554
TspDTI ATGAA 4 cut(s) 217, 305, 501, 1055
TspGWI ACGGA 3 cut(s) 132, 846, 1140
TspRI CASTG 2 cut(s) 886, 1143
Tth111I GACNNNGTC 1 cut(s) 985
VspI ATTAAT 1 cut(s) 794
XagI CCTNNNNNAGG 2 cut(s) 573, 703
XapI RAATTY 3 cut(s) 875, 898, 1251
XceI RCATGY 1 cut(s) 733
XmiI GTMKAC 1 cut(s) 944
XspI CTAG 3 cut(s) 17, 705, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.