Rmu_sc0003610.1_g000045

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003610.1
OG0002076
Physical Location & Seq
Forward (+)
191110 .. 193574
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003610.1_g000045.1.cds

Sequence Viewer

Length: 762 bp
atggccgatattgctgttgaaatggctgcttctaactcacagccatcgggtaatcaaccttctaggtcaagatacgatctgatgtataagcgaggacgagactcggaaagtgacgtaagaaataccatgttggtggtggctgccctaattacagccgcaacctttgcagccggcatgaacccacctgggtttggcaagaagataacgtttctcataaacagtattcggcctacggtgcttcggttgccgcagtatgtcctcccggagatcatttctaccgcggttgccatagcctttccctatatggtgacgattattgctgatttgtggaacaaatggcgcaaggggaagaaagagagagaaagaaagaaagagagagtgaagaggttgggccatgccagaacaatggggtgcccagagtatttgtcgcatgatgccgtggcgcgtgagctggtagtactaggctacgtcaagttcgacatatcactgagaccatctcagaatgattcagaggatgatctttctattgatggaaagcttcgaatatctagtttccagatctttttacggcttgtcaatatcatttttctagaagaagttatggccaatttagtacaagaaggtcaggctgttagtgaatctgaggttggacgagaatttatgtttcgaagcccaaatcacactgagaagcccaaggacgattttgtgcttcatattccgacttatgatggacaaacggcacgatatgaatggttggaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGG

Protein Analysis

253

Amino Acids

28.73

Weight (kDa)

8.56

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 411
AccII CGCG 2 cut(s) 281, 445
AciI CCGC 4 cut(s) 156, 248, 279, 281
AclI AACGTT 1 cut(s) 206
AcoI YGGCCR 2 cut(s) 3, 603
AcsI RAATTY 1 cut(s) 656
AfaI GTAC 2 cut(s) 459, 615
AfiI CCNNNNNNNGG 1 cut(s) 191
AgsI TTSAA 1 cut(s) 20
AjnI CCWGG 1 cut(s) 184
AjuI GAANNNNNNNTTGG 2 cut(s) 630, 662
AluBI AGCT 2 cut(s) 451, 538
AluI AGCT 2 cut(s) 451, 538
Alw26I GTCTC 2 cut(s) 93, 484
AoxI GGCC 4 cut(s) 3, 227, 391, 603
ApeKI GCWGC 3 cut(s) 26, 140, 167
ApoI RAATTY 1 cut(s) 656
AspLEI GCGC 2 cut(s) 342, 445
AspS9I GGNCC 1 cut(s) 391
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 2 cut(s) 541, 667
BaeGI GKGCMC 1 cut(s) 416
BalI TGGCCA 1 cut(s) 605
BanI GGYRCC 1 cut(s) 411
BarI GAAGNNNNNNTAC 2 cut(s) 43, 75
BbvI GCAGC 3 cut(s) 13, 127, 179
BccI CCATC 4 cut(s) 52, 502, 524, 722
BceAI ACGGC 3 cut(s) 422, 584, 753
BciT130I CCWGG 1 cut(s) 186
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 2 cut(s) 93, 484
BfaI CTAG 4 cut(s) 63, 461, 549, 590
BglII AGATCT 1 cut(s) 558
BisI GCNGC 5 cut(s) 27, 141, 156, 168, 248
BlsI GCNGC 5 cut(s) 28, 142, 157, 169, 249
BmcAI AGTACT 1 cut(s) 459
Bme1390I CCNGG 2 cut(s) 186, 263
BmgT120I GGNCC 1 cut(s) 391
BmiI GGNNCC 1 cut(s) 413
BmrFI CCNGG 2 cut(s) 186, 263
BmsI GCATC 1 cut(s) 424
BplI GAGNNNNNCTC 2 cut(s) 481, 513
Bpu14I TTCGAA 2 cut(s) 541, 667
BpuMI CCSGG 1 cut(s) 263
BsaI GGTCTC 1 cut(s) 484
BsaJI CCNNGG 4 cut(s) 185, 279, 438, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 191
Bse118I RCCGGY 1 cut(s) 170
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 4 cut(s) 185, 279, 438, 693
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 1 cut(s) 191
BseMII CTCAG 4 cut(s) 479, 512, 633, 675
BseSI GKGCMC 1 cut(s) 416
BseXI GCAGC 3 cut(s) 13, 127, 179
Bsh1236I CGCG 2 cut(s) 281, 445
BshFI GGCC 4 cut(s) 5, 229, 393, 605
BshNI GGYRCC 1 cut(s) 411
BsiSI CCGG 2 cut(s) 171, 263
BslI CCNNNNNNNGG 1 cut(s) 191
BsmAI GTCTC 2 cut(s) 93, 484
BsnI GGCC 4 cut(s) 5, 229, 393, 605
Bso31I GGTCTC 1 cut(s) 484
Bsp119I TTCGAA 2 cut(s) 541, 667
Bsp1286I GDGCHC 1 cut(s) 416
Bsp143I GATC 4 cut(s) 76, 267, 517, 558
BspACI CCGC 4 cut(s) 156, 248, 279, 281
BspANI GGCC 4 cut(s) 5, 229, 393, 605
BspCNI CTCAG 4 cut(s) 480, 511, 634, 676
BspFNI CGCG 2 cut(s) 281, 445
BspLI GGNNCC 1 cut(s) 413
BspT104I TTCGAA 2 cut(s) 541, 667
BspT107I GGYRCC 1 cut(s) 411
BspTNI GGTCTC 1 cut(s) 484
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 4 cut(s) 185, 279, 438, 693
BssMI GATC 4 cut(s) 76, 267, 517, 558
BssT1I CCWWGG 1 cut(s) 693
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 221, 235
Bst6I CTCTTC 1 cut(s) 377
BstAPI GCANNNNNTGC 1 cut(s) 164
BstBI TTCGAA 2 cut(s) 541, 667
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 4 cut(s) 488, 498, 642, 684
BstDSI CCRYGG 2 cut(s) 279, 438
BstF5I GGATG 1 cut(s) 520
BstFNI CGCG 2 cut(s) 281, 445
BstHHI GCGC 2 cut(s) 342, 445
BstKTI GATC 4 cut(s) 79, 270, 520, 561
BstMAI GTCTC 2 cut(s) 93, 484
BstMBI GATC 4 cut(s) 76, 267, 517, 558
BstMWI GCNNNNNNNGC 4 cut(s) 11, 164, 235, 244
BstNI CCWGG 1 cut(s) 186
BstSCI CCNGG 2 cut(s) 184, 261
BstSLI GKGCMC 1 cut(s) 416
BstUI CGCG 2 cut(s) 281, 445
BstV1I GCAGC 3 cut(s) 13, 127, 179
BstX2I RGATCY 1 cut(s) 558
BstXI CCANNNNNNTGG 2 cut(s) 133, 406
BstYI RGATCY 1 cut(s) 558
BsuRI GGCC 4 cut(s) 5, 229, 393, 605
BtgI CCRYGG 2 cut(s) 279, 438
BtsCI GGATG 1 cut(s) 520
BtsIMutI CAGTG 2 cut(s) 485, 681
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 2 cut(s) 342, 445
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 391
Cfr42I CCGCGG 1 cut(s) 282
Csp6I GTAC 2 cut(s) 458, 614
CviAII CATG 4 cut(s) 127, 175, 395, 431
CviQI GTAC 2 cut(s) 458, 614
DdeI CTNAG 4 cut(s) 488, 498, 642, 684
DpnI GATC 4 cut(s) 78, 269, 519, 560
DpnII GATC 4 cut(s) 76, 267, 517, 558
EaeI YGGCCR 2 cut(s) 3, 603
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 693
Eco31I GGTCTC 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 693
ErhI CCWWGG 1 cut(s) 693
FaeI CATG 4 cut(s) 130, 178, 398, 434
FatI CATG 4 cut(s) 126, 174, 394, 430
Fnu4HI GCNGC 5 cut(s) 27, 141, 156, 168, 248
FokI GGATG 1 cut(s) 527
Fsp4HI GCNGC 5 cut(s) 27, 141, 156, 168, 248
FspBI CTAG 4 cut(s) 63, 461, 549, 590
GlaI GCGC 2 cut(s) 341, 444
GluI GCNGC 5 cut(s) 27, 141, 156, 168, 248
HaeIII GGCC 4 cut(s) 5, 229, 393, 605
HapII CCGG 2 cut(s) 171, 263
HhaI GCGC 2 cut(s) 342, 445
Hin1II CATG 4 cut(s) 130, 178, 398, 434
Hin6I GCGC 2 cut(s) 340, 443
HinP1I GCGC 2 cut(s) 340, 443
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 3 cut(s) 101, 506, 638
HpaII CCGG 2 cut(s) 171, 263
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 6 cut(s) 81, 106, 501, 511, 643, 720
Hpy188III TCNNGA 3 cut(s) 69, 556, 590
HpyAV CCTTC 2 cut(s) 69, 614
HpyCH4III ACNGT 2 cut(s) 221, 235
HpyCH4IV ACGT 3 cut(s) 114, 206, 468
HpyCH4V TGCA 1 cut(s) 167
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 164, 235, 244
HpyF3I CTNAG 4 cut(s) 488, 498, 642, 684
HpySE526I ACGT 3 cut(s) 114, 206, 468
Hsp92II CATG 4 cut(s) 130, 178, 398, 434
HspAI GCGC 2 cut(s) 340, 443
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
KspI CCGCGG 1 cut(s) 282
Kzo9I GATC 4 cut(s) 76, 267, 517, 558
LpnPI CCDG 9 cut(s) 171, 184, 198, 276, 412, 429, 437, 569, 611
Lsp1109I GCAGC 3 cut(s) 13, 127, 179
LweI GCATC 1 cut(s) 424
MaeI CTAG 4 cut(s) 63, 461, 549, 590
MaeII ACGT 3 cut(s) 114, 206, 468
MaeIII GTNAC 2 cut(s) 110, 307
MalI GATC 4 cut(s) 78, 269, 519, 560
MboI GATC 4 cut(s) 76, 267, 517, 558
MboII GAAGA 4 cut(s) 211, 361, 394, 605
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 1 cut(s) 416
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 3 cut(s) 147, 607, 656
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 3 cut(s) 628, 735, 743
MnlI CCTC 5 cut(s) 86, 269, 378, 505, 637
Mox20I TGGCCA 1 cut(s) 605
MroNI GCCGGC 1 cut(s) 170
MscI TGGCCA 1 cut(s) 605
MslI CAYNNNNRTG 1 cut(s) 131
Msp20I TGGCCA 1 cut(s) 605
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 2 cut(s) 171, 263
MspR9I CCNGG 2 cut(s) 186, 263
MvaI CCWGG 1 cut(s) 186
MvnI CGCG 2 cut(s) 281, 445
MwoI GCNNNNNNNGC 4 cut(s) 11, 164, 235, 244
NaeI GCCGGC 1 cut(s) 172
NciI CCSGG 1 cut(s) 263
NdeII GATC 4 cut(s) 76, 267, 517, 558
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 4 cut(s) 130, 178, 398, 434
NlaIV GGNNCC 1 cut(s) 413
NmuCI GTSAC 2 cut(s) 110, 307
NspV TTCGAA 2 cut(s) 541, 667
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 2 cut(s) 506, 638
PfoI TCCNGGA 1 cut(s) 261
PkrI GCNGC 5 cut(s) 28, 142, 157, 169, 249
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp1406I AACGTT 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 1 cut(s) 413
PspPI GGNCC 1 cut(s) 391
PsuI RGATCY 1 cut(s) 558
RsaI GTAC 2 cut(s) 459, 615
RsaNI GTAC 2 cut(s) 458, 614
RseI CAYNNNNRTG 1 cut(s) 131
SacII CCGCGG 1 cut(s) 282
SatI GCNGC 5 cut(s) 27, 141, 156, 168, 248
Sau3AI GATC 4 cut(s) 76, 267, 517, 558
Sau96I GGNCC 1 cut(s) 391
ScaI AGTACT 1 cut(s) 459
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 2 cut(s) 186, 263
SduI GDGCHC 1 cut(s) 416
SfaNI GCATC 1 cut(s) 424
Sfr303I CCGCGG 1 cut(s) 282
SfuI TTCGAA 2 cut(s) 541, 667
SgrBI CCGCGG 1 cut(s) 282
SmiMI CAYNNNNRTG 1 cut(s) 131
Sse9I AATT 3 cut(s) 147, 607, 656
SsiI CCGC 4 cut(s) 156, 248, 279, 281
SspMI CTAG 4 cut(s) 63, 461, 549, 590
StyD4I CCNGG 2 cut(s) 184, 261
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 2 cut(s) 221, 235
TaiI ACGT 3 cut(s) 117, 209, 471
TaqI TCGA 3 cut(s) 477, 541, 667
TasI AATT 3 cut(s) 147, 607, 656
TatI WGTACW 2 cut(s) 457, 613
TauI GCSGC 2 cut(s) 158, 250
TfiI GAWTC 2 cut(s) 506, 638
TscAI CASTG 2 cut(s) 492, 688
TseFI GTSAC 2 cut(s) 110, 307
TseI GCWGC 3 cut(s) 26, 140, 167
Tsp45I GTSAC 2 cut(s) 110, 307
TspDTI ATGAA 3 cut(s) 191, 701, 762
TspRI CASTG 2 cut(s) 492, 688
XapI RAATTY 1 cut(s) 656
XbaI TCTAGA 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XspI CTAG 4 cut(s) 63, 461, 549, 590
ZrmI AGTACT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.