Rroxscaffold_3G00270170

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002076
Physical Location & Seq
Reverse (-)
62800284 .. 62800961
678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270170.1

Sequence Viewer

Length: 612 bp
ATGGGGAAGTCGCGTGAAATACTTAATCGAGATGATGAAGCTTCTTTAGAAAATCGATGCATGAATCATCCATTCTACCATGAGCTAATGGCCGATATTGCTGTTGAAATGGCTGCTTCTAATTCACAGCCATCGGGTAATCAACCTTCTAGGTCGAGATACGATCTGACGTATAAGCCAGGACGAGACTCGGAAAGTGACGTAAGAAATACCATGTTGGTGGTGGCTGCCCTAATTACAGCCGCAACCTTTGCAGCCGGCATGAACCCACCTGGCGGAGTTTGGCAAGAAGATAACGTTTCTCGTAAACAGTATTCGGGTAAGTCGATTTTAGGTACTCAAAGCGTTACTACATACCGAGTGTTCTTCTTGTTGAACTCGACAGCTTTTTCATCGGCTACACTTGTAATAAACTACCTTGTGTGGAACTTTCCATATTCTCCGGAGATATGGGGTGCTCTGGGTTGTATGGCGGCAACCTACGGTGCTTCAGTTGCCGCAGTAAGTCCTCCCGGAGATCATTTCTACGGCCGGTTCATATGGGTTGCCATAGCATTTCCCTATATGGGGCGGATTATTGCTGATTTGTGGAACAAATGGCGCAAGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGG

Protein Analysis

203

Amino Acids

22.53

Weight (kDa)

7.76

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 66 - 143 1.3e-19 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 13
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 5 cut(s) 243, 276, 473, 498, 571
AclI AACGTT 1 cut(s) 297
AcoI YGGCCR 2 cut(s) 90, 529
AcuI CTGAAG 1 cut(s) 474
AfaI GTAC 1 cut(s) 337
AfiI CCNNNNNNNGG 3 cut(s) 275, 566, 567
AgsI TTSAA 2 cut(s) 107, 376
AjnI CCWGG 2 cut(s) 178, 271
AluBI AGCT 3 cut(s) 41, 85, 386
AluI AGCT 3 cut(s) 41, 85, 386
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 1 cut(s) 180
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 2 cut(s) 90, 529
ApeKI GCWGC 3 cut(s) 113, 227, 254
AspLEI GCGC 1 cut(s) 603
AsuC2I CCSGG 1 cut(s) 513
BarI GAAGNNNNNNTAC 2 cut(s) 130, 162
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 3 cut(s) 100, 214, 266
BccI CCATC 1 cut(s) 139
BceAI ACGGC 1 cut(s) 544
BciT130I CCWGG 2 cut(s) 180, 273
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 1 cut(s) 150
BisI GCNGC 6 cut(s) 114, 228, 243, 255, 474, 498
BlsI GCNGC 6 cut(s) 115, 229, 244, 256, 475, 499
Bme1390I CCNGG 3 cut(s) 180, 273, 513
BmrFI CCNGG 3 cut(s) 180, 273, 513
BmsI GCATC 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 513
Bsa29I ATCGAT 1 cut(s) 55
BsaWI WCCGGW 1 cut(s) 442
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 566, 567
Bse118I RCCGGY 2 cut(s) 257, 531
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 2 cut(s) 180, 273
BseCI ATCGAT 1 cut(s) 55
BseGI GGATG 1 cut(s) 67
BseLI CCNNNNNNNGG 3 cut(s) 275, 566, 567
BseX3I CGGCCG 1 cut(s) 529
BseXI GCAGC 3 cut(s) 100, 214, 266
Bsh1236I CGCG 1 cut(s) 13
Bsh1285I CGRYCG 1 cut(s) 532
BshFI GGCC 2 cut(s) 92, 531
BshVI ATCGAT 1 cut(s) 55
BsiEI CGRYCG 1 cut(s) 532
BsiHKAI GWGCWC 1 cut(s) 460
BsiSI CCGG 4 cut(s) 258, 443, 513, 532
BslI CCNNNNNNNGG 3 cut(s) 275, 566, 567
BsmAI GTCTC 1 cut(s) 180
BsnI GGCC 2 cut(s) 92, 531
Bsp1286I GDGCHC 1 cut(s) 460
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 2 cut(s) 163, 517
BspACI CCGC 5 cut(s) 243, 276, 473, 498, 571
BspANI GGCC 2 cut(s) 92, 531
BspDI ATCGAT 1 cut(s) 55
BspEI TCCGGA 1 cut(s) 442
BspFNI CGCG 1 cut(s) 13
BsrFI RCCGGY 2 cut(s) 257, 531
BssAI RCCGGY 2 cut(s) 257, 531
BssMI GATC 2 cut(s) 163, 517
Bst2UI CCWGG 2 cut(s) 180, 273
Bst4CI ACNGT 2 cut(s) 312, 485
BstAPI GCANNNNNTGC 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 259
BstF5I GGATG 1 cut(s) 67
BstFNI CGCG 1 cut(s) 13
BstHHI GCGC 1 cut(s) 603
BstKTI GATC 2 cut(s) 166, 520
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 2 cut(s) 163, 517
BstMCI CGRYCG 1 cut(s) 532
BstMWI GCNNNNNNNGC 3 cut(s) 98, 251, 494
BstNI CCWGG 2 cut(s) 180, 273
BstSCI CCNGG 3 cut(s) 178, 271, 511
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 3 cut(s) 100, 214, 266
BstXI CCANNNNNNTGG 1 cut(s) 220
BstZI CGGCCG 1 cut(s) 529
Bsu15I ATCGAT 1 cut(s) 55
BsuRI GGCC 2 cut(s) 92, 531
BsuTUI ATCGAT 1 cut(s) 55
BtsCI GGATG 1 cut(s) 67
Cac8I GCNNGC 1 cut(s) 259
CfoI GCGC 1 cut(s) 603
Cfr10I RCCGGY 2 cut(s) 257, 531
ClaI ATCGAT 1 cut(s) 55
Csp6I GTAC 1 cut(s) 336
CviAII CATG 4 cut(s) 61, 80, 214, 262
CviQI GTAC 1 cut(s) 336
DpnI GATC 2 cut(s) 165, 519
DpnII GATC 2 cut(s) 163, 517
EaeI YGGCCR 2 cut(s) 90, 529
EagI CGGCCG 1 cut(s) 529
EciI GGCGGA 2 cut(s) 291, 586
EclXI CGGCCG 1 cut(s) 529
Eco52I CGGCCG 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 474
EcoRII CCWGG 2 cut(s) 178, 271
EcoT22I ATGCAT 1 cut(s) 62
FaeI CATG 4 cut(s) 64, 83, 217, 265
FatI CATG 4 cut(s) 60, 79, 213, 261
FauNDI CATATG 1 cut(s) 539
Fnu4HI GCNGC 6 cut(s) 114, 228, 243, 255, 474, 498
FokI GGATG 1 cut(s) 54
Fsp4HI GCNGC 6 cut(s) 114, 228, 243, 255, 474, 498
FspBI CTAG 1 cut(s) 150
GlaI GCGC 1 cut(s) 602
GluI GCNGC 6 cut(s) 114, 228, 243, 255, 474, 498
HaeIII GGCC 2 cut(s) 92, 531
HapII CCGG 4 cut(s) 258, 443, 513, 532
HhaI GCGC 1 cut(s) 603
Hin1II CATG 4 cut(s) 64, 83, 217, 265
Hin6I GCGC 1 cut(s) 601
HinP1I GCGC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 2 cut(s) 64, 188
HpaII CCGG 4 cut(s) 258, 443, 513, 532
Hpy166II GTNNAC 1 cut(s) 308
Hpy188I TCNGA 2 cut(s) 168, 193
Hpy188III TCNNGA 3 cut(s) 29, 156, 443
Hpy8I GTNNAC 1 cut(s) 308
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 2 cut(s) 312, 485
HpyCH4IV ACGT 3 cut(s) 170, 201, 297
HpyCH4V TGCA 2 cut(s) 60, 254
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 251, 494
HpySE526I ACGT 3 cut(s) 170, 201, 297
Hsp92II CATG 4 cut(s) 64, 83, 217, 265
HspAI GCGC 1 cut(s) 601
Kpn2I TCCGGA 1 cut(s) 442
KroI GCCGGC 1 cut(s) 257
KroNI GCCGGC 1 cut(s) 259
Kzo9I GATC 2 cut(s) 163, 517
LpnPI CCDG 9 cut(s) 165, 192, 258, 271, 285, 446, 456, 526, 545
Lsp1109I GCAGC 3 cut(s) 100, 214, 266
LweI GCATC 1 cut(s) 47
MaeI CTAG 1 cut(s) 150
MaeII ACGT 3 cut(s) 170, 201, 297
MaeIII GTNAC 2 cut(s) 197, 346
MalI GATC 2 cut(s) 165, 519
MboI GATC 2 cut(s) 163, 517
MboII GAAGA 2 cut(s) 302, 358
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 2 cut(s) 121, 234
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 1 cut(s) 519
Mph1103I ATGCAT 1 cut(s) 62
MroI TCCGGA 1 cut(s) 442
MroNI GCCGGC 1 cut(s) 257
MseI TTAA 1 cut(s) 24
MslI CAYNNNNRTG 1 cut(s) 218
MspI CCGG 4 cut(s) 258, 443, 513, 532
MspR9I CCNGG 3 cut(s) 180, 273, 513
MvaI CCWGG 2 cut(s) 180, 273
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 3 cut(s) 98, 251, 494
NaeI GCCGGC 1 cut(s) 259
NciI CCSGG 1 cut(s) 513
NdeI CATATG 1 cut(s) 539
NdeII GATC 2 cut(s) 163, 517
NgoMIV GCCGGC 1 cut(s) 257
NlaIII CATG 4 cut(s) 64, 83, 217, 265
NmuCI GTSAC 1 cut(s) 197
NsiI ATGCAT 1 cut(s) 62
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PdiI GCCGGC 1 cut(s) 259
PfeI GAWTC 1 cut(s) 64
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 6 cut(s) 115, 229, 244, 256, 475, 499
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp1406I AACGTT 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 178, 271
PspGI CCWGG 2 cut(s) 178, 271
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 1 cut(s) 24
SatI GCNGC 6 cut(s) 114, 228, 243, 255, 474, 498
Sau3AI GATC 2 cut(s) 163, 517
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 180, 273, 513
SduI GDGCHC 1 cut(s) 460
SfaNI GCATC 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 218
Sse9I AATT 2 cut(s) 121, 234
SsiI CCGC 5 cut(s) 243, 276, 473, 498, 571
SspMI CTAG 1 cut(s) 150
StyD4I CCNGG 3 cut(s) 178, 271, 511
TaaI ACNGT 2 cut(s) 312, 485
TaiI ACGT 3 cut(s) 173, 204, 300
TaqI TCGA 5 cut(s) 28, 55, 155, 326, 380
TasI AATT 2 cut(s) 121, 234
TauI GCSGC 3 cut(s) 245, 476, 500
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 3 cut(s) 113, 227, 254
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 5 cut(s) 51, 77, 278, 381, 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XspI CTAG 1 cut(s) 150
Zsp2I ATGCAT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.