Rw2G029800

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002076
Physical Location & Seq
Forward (+)
48311710 .. 48313920
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029800.1

Sequence Viewer

Length: 549 bp
ATGGCCGACAGTAATGATGTTGAAATGGCTGCTCCACAGCCAACTGGTGATCAAACTACTTCTAGGTCGAAATATGATCTTATGTACGATCCAAAACGAGACACCGCAAAAGACGTAAGAAATGCTATGTTAGTCGTCGCTGCCCTTATTTCTGCGGCAACCTTCGCAGCGGGAATAAACCCTCCAGGCGGGGTTTGGCAAGGCAATATCGAAAATTCCACTCAACACTCTTCCGGTAAGTCGGTTTTAGGTACTAATAGTGCACTTGATTACAAAGTCTTCTATTTCTTGAACGCCGTAGCTTTCTCGTCAGCAAACGTGGTGATTAGCAATCTTGTTTATAGATTTCCATATTATCCGGAAATATGGGCTGCTATGTTTTCTATGTCGGGAACCTACGGTGTTTCGGTTGCTGCCGTAAGTCCCCCTGGAGATGCTTTCTACGGTCGGTTCATATGGTTTGCCATCGCCGTTCCTTATATTATAAGGATTATTGTTCACTATTGGAAGAAATGGTCCACCGACGTCGGGTTTCAAGTTGAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGG

Protein Analysis

182

Amino Acids

19.98

Weight (kDa)

6.06

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 36 - 116 6.2e-17 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 485
AatII GACGTC 1 cut(s) 528
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 4 cut(s) 105, 155, 170, 189
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 214
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 2 cut(s) 86, 253
AfiI CCNNNNNNNGG 2 cut(s) 188, 528
AgsI TTSAA 4 cut(s) 23, 292, 536, 542
AjnI CCWGG 2 cut(s) 184, 427
AluBI AGCT 1 cut(s) 302
AluI AGCT 1 cut(s) 302
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 1 cut(s) 93
Alw44I GTGCAC 1 cut(s) 261
AlwI GGATC 1 cut(s) 83
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 261
ApeKI GCWGC 5 cut(s) 29, 140, 167, 371, 413
ApoI RAATTY 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 516
AsuHPI GGTGA 2 cut(s) 59, 334
AvaII GGWCC 1 cut(s) 516
BaeGI GKGCMC 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 5 cut(s) 16, 127, 179, 358, 400
BccI CCATC 1 cut(s) 473
BceAI ACGGC 3 cut(s) 281, 401, 455
BciT130I CCWGG 2 cut(s) 186, 429
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 1 cut(s) 63
BisI GCNGC 6 cut(s) 30, 141, 156, 168, 372, 414
BlsI GCNGC 6 cut(s) 31, 142, 157, 169, 373, 415
Bme1390I CCNGG 2 cut(s) 186, 429
Bme18I GGWCC 1 cut(s) 516
BmgT120I GGNCC 1 cut(s) 516
BmiI GGNNCC 1 cut(s) 394
BmrFI CCNGG 2 cut(s) 186, 429
BmsI GCATC 1 cut(s) 424
BpiI GAAGAC 1 cut(s) 271
BpmI CTGGAG 2 cut(s) 168, 450
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 427
BsaWI WCCGGW 2 cut(s) 233, 358
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 528
Bse1I ACTGG 1 cut(s) 49
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 2 cut(s) 186, 429
BseDI CCNNGG 1 cut(s) 427
BseLI CCNNNNNNNGG 2 cut(s) 188, 528
BseNI ACTGG 1 cut(s) 49
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 5 cut(s) 16, 127, 179, 358, 400
Bsh1285I CGRYCG 1 cut(s) 448
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 448
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 2 cut(s) 234, 359
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 2 cut(s) 188, 528
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 408
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 265
Bsp13I TCCGGA 1 cut(s) 358
Bsp143I GATC 3 cut(s) 49, 76, 88
BspACI CCGC 4 cut(s) 105, 155, 170, 189
BspANI GGCC 1 cut(s) 5
BspEI TCCGGA 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 394
BspPI GGATC 1 cut(s) 83
BsrI ACTGG 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 3 cut(s) 49, 76, 88
BssNI GRCGYC 1 cut(s) 525
Bst2UI CCWGG 2 cut(s) 186, 429
Bst4CI ACNGT 3 cut(s) 11, 401, 446
Bst6I CTCTTC 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 525
BstKTI GATC 3 cut(s) 52, 79, 91
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 3 cut(s) 49, 76, 88
BstMCI CGRYCG 1 cut(s) 448
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 2 cut(s) 186, 429
BstSCI CCNGG 2 cut(s) 184, 427
BstSLI GKGCMC 1 cut(s) 265
BstV1I GCAGC 5 cut(s) 16, 127, 179, 358, 400
BstV2I GAAGAC 1 cut(s) 271
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 451
Cfr13I GGNCC 1 cut(s) 516
Csp6I GTAC 2 cut(s) 85, 252
CviJI RGCY 5 cut(s) 5, 29, 40, 302, 371
CviKI_1 RGCY 5 cut(s) 5, 29, 40, 302, 371
CviQI GTAC 2 cut(s) 85, 252
DpnI GATC 3 cut(s) 51, 78, 90
DpnII GATC 3 cut(s) 49, 76, 88
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 516
EcoRII CCWGG 2 cut(s) 184, 427
FaqI GGGAC 1 cut(s) 408
FauI CCCGC 2 cut(s) 163, 182
FauNDI CATATG 1 cut(s) 455
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 6 cut(s) 30, 141, 156, 168, 372, 414
Fsp4HI GCNGC 6 cut(s) 30, 141, 156, 168, 372, 414
FspBI CTAG 1 cut(s) 63
GluI GCNGC 6 cut(s) 30, 141, 156, 168, 372, 414
GsuI CTGGAG 2 cut(s) 168, 450
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 234, 359
Hin1I GRCGYC 1 cut(s) 525
HpaII CCGG 2 cut(s) 234, 359
HphI GGTGA 2 cut(s) 59, 334
Hpy166II GTNNAC 3 cut(s) 263, 499, 519
Hpy188III TCNNGA 3 cut(s) 289, 359, 390
Hpy8I GTNNAC 3 cut(s) 263, 499, 519
Hpy99I CGWCG 3 cut(s) 140, 527, 530
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 3 cut(s) 11, 401, 446
HpyCH4IV ACGT 3 cut(s) 114, 318, 525
HpyCH4V TGCA 1 cut(s) 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpySE526I ACGT 3 cut(s) 114, 318, 525
Hsp92I GRCGYC 1 cut(s) 525
Kpn2I TCCGGA 1 cut(s) 358
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 3 cut(s) 49, 76, 88
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 7 cut(s) 30, 171, 198, 247, 372, 414, 441
Lsp1109I GCAGC 5 cut(s) 16, 127, 179, 358, 400
LweI GCATC 1 cut(s) 424
MaeI CTAG 1 cut(s) 63
MaeII ACGT 3 cut(s) 114, 318, 525
MalI GATC 3 cut(s) 51, 78, 90
MboI GATC 3 cut(s) 49, 76, 88
MboII GAAGA 3 cut(s) 222, 271, 520
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 1 cut(s) 214
MnlI CCTC 1 cut(s) 192
MroI TCCGGA 1 cut(s) 358
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 234, 359
MspR9I CCNGG 2 cut(s) 186, 429
MvaI CCWGG 2 cut(s) 186, 429
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeI CATATG 1 cut(s) 455
NdeII GATC 3 cut(s) 49, 76, 88
NlaIV GGNNCC 1 cut(s) 394
PkrI GCNGC 6 cut(s) 31, 142, 157, 169, 373, 415
PsiI TTATAA 1 cut(s) 485
Psp6I CCWGG 2 cut(s) 184, 427
PspGI CCWGG 2 cut(s) 184, 427
PspN4I GGNNCC 1 cut(s) 394
PspPI GGNCC 1 cut(s) 516
RsaI GTAC 2 cut(s) 86, 253
RsaNI GTAC 2 cut(s) 85, 252
SatI GCNGC 6 cut(s) 30, 141, 156, 168, 372, 414
Sau3AI GATC 3 cut(s) 49, 76, 88
Sau96I GGNCC 1 cut(s) 516
ScrFI CCNGG 2 cut(s) 186, 429
SduI GDGCHC 1 cut(s) 265
SetI ASST 8 cut(s) 68, 117, 164, 253, 304, 321, 398, 528
SfaNI GCATC 1 cut(s) 424
SinI GGWCC 1 cut(s) 516
Sse9I AATT 1 cut(s) 214
SsiI CCGC 4 cut(s) 105, 155, 170, 189
SspMI CTAG 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 184, 427
TaaI ACNGT 3 cut(s) 11, 401, 446
TaiI ACGT 3 cut(s) 117, 321, 528
TaqI TCGA 2 cut(s) 68, 210
TasI AATT 1 cut(s) 214
TauI GCSGC 1 cut(s) 158
TseI GCWGC 5 cut(s) 29, 140, 167, 371, 413
TspDTI ATGAA 1 cut(s) 442
VneI GTGCAC 1 cut(s) 261
VpaK11BI GGWCC 1 cut(s) 516
XapI RAATTY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 192
XspI CTAG 1 cut(s) 63
ZraI GACGTC 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.