Rmu_sc0004193.1_g000008

Floral homeotic protein APETALA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004193.1
OG0002429
Physical Location & Seq
Forward (+)
27290 .. 28587
1298 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 474 bp
atggagaatatacttgaacgctatgagcggtactcgtacgcagagagacagctagtcgaacctgatttggaatcacagggtaactggaccttcgaacatgctagactgaaggtgaaagttgagcttctgcaaagaaaccttaggcactatttgggagaagatctggattcattgagtatcaaagagatccaaagtttggagcaacagcttgacaattctcttaagcaaattcgatcaagaaagattaaggagaaagagaagaatgtagcggaagctcaggaggttcataattgggagcagcagcaactgcagcaaaaccatggccttaacctggtagcacaggctccacttccatgtttgaacatggggggcactcagcaaaatgatcagttccttcaagtgaggaggaaccagctcgaccttactctcgagccatcgttgtattcatgccaccttggatgctttgcttcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

157

Amino Acids

18.45

Weight (kDa)

5.56

Isoelectric Point (pI)

59.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 25 - 109 5.1e-19 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 196
AccBSI CCGCTC 1 cut(s) 28
AciI CCGC 2 cut(s) 28, 269
AclWI GGATC 1 cut(s) 181
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 128
AfaI GTAC 2 cut(s) 32, 38
AfiI CCNNNNNNNGG 2 cut(s) 196, 331
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 3 cut(s) 17, 361, 398
AhdI GACNNNNNGTC 1 cut(s) 53
AjnI CCWGG 1 cut(s) 330
AluBI AGCT 5 cut(s) 52, 124, 208, 275, 415
AluI AGCT 5 cut(s) 52, 124, 208, 275, 415
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 1 cut(s) 181
AlwNI CAGNNNCTG 1 cut(s) 307
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 3 cut(s) 298, 301, 310
ApoI RAATTY 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 93
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 1 cut(s) 87
AxyI CCTNAGG 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 374
BbvI GCAGC 3 cut(s) 310, 313, 322
BccI CCATC 1 cut(s) 442
BciT130I CCWGG 1 cut(s) 332
BclI TGATCA 1 cut(s) 385
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 2 cut(s) 53, 102
BfmI CTRYAG 1 cut(s) 308
BfrI CTTAAG 1 cut(s) 221
BglII AGATCT 1 cut(s) 160
BisI GCNGC 3 cut(s) 299, 302, 311
BlsI GCNGC 3 cut(s) 300, 303, 312
Bme1390I CCNGG 1 cut(s) 332
Bme18I GGWCC 1 cut(s) 87
BmeRI GACNNNNNGTC 1 cut(s) 53
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 2 cut(s) 345, 410
BmrFI CCNGG 1 cut(s) 332
BmsI GCATC 1 cut(s) 449
BplI GAGNNNNNCTC 2 cut(s) 17, 49
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 319, 454
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 331
Bse1I ACTGG 1 cut(s) 89
Bse21I CCTNAGG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 2 cut(s) 319, 454
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 2 cut(s) 196, 331
BseMII CTCAG 2 cut(s) 290, 389
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 418
BseSI GKGCMC 1 cut(s) 374
BseXI GCAGC 3 cut(s) 310, 313, 322
BshFI GGCC 1 cut(s) 324
BsiHKCI CYCGRG 1 cut(s) 428
BsiWI CGTACG 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 196, 331
BsmAI GTCTC 1 cut(s) 40
BsnI GGCC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 428
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 374
Bsp143I GATC 4 cut(s) 160, 186, 233, 385
Bsp19I CCATGG 1 cut(s) 319
BspACI CCGC 2 cut(s) 28, 269
BspANI GGCC 1 cut(s) 324
BspCNI CTCAG 2 cut(s) 289, 388
BspHI TCATGA 1 cut(s) 470
BspLI GGNNCC 2 cut(s) 345, 410
BspMAI CTGCAG 1 cut(s) 312
BspPI GGATC 1 cut(s) 181
BspT104I TTCGAA 1 cut(s) 93
BspTI CTTAAG 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 28
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 319, 454
BssMI GATC 4 cut(s) 160, 186, 233, 385
BssT1I CCWWGG 2 cut(s) 319, 454
Bst2UI CCWGG 1 cut(s) 332
BstAFI CTTAAG 1 cut(s) 221
BstAPI GCANNNNNTGC 1 cut(s) 307
BstBI TTCGAA 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 140, 276, 375
BstDSI CCRYGG 1 cut(s) 319
BstF5I GGATG 1 cut(s) 464
BstKTI GATC 4 cut(s) 163, 189, 236, 388
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 4 cut(s) 160, 186, 233, 385
BstMWI GCNNNNNNNGC 2 cut(s) 307, 310
BstNI CCWGG 1 cut(s) 332
BstNSI RCATGY 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 330
BstSFI CTRYAG 1 cut(s) 308
BstSLI GKGCMC 1 cut(s) 374
BstV1I GCAGC 3 cut(s) 310, 313, 322
BstX2I RGATCY 2 cut(s) 160, 186
BstYI RGATCY 2 cut(s) 160, 186
Bsu36I CCTNAGG 1 cut(s) 140
BsuRI GGCC 1 cut(s) 324
BtgI CCRYGG 1 cut(s) 319
BtsCI GGATG 1 cut(s) 464
CaiI CAGNNNCTG 1 cut(s) 307
CciI TCATGA 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 87
CsiI ACCWGGT 1 cut(s) 330
Csp6I GTAC 2 cut(s) 31, 37
CviAII CATG 6 cut(s) 98, 320, 354, 364, 447, 471
CviJI RGCY 8 cut(s) 52, 124, 208, 275, 324, 344, 415, 433
CviKI_1 RGCY 8 cut(s) 52, 124, 208, 275, 324, 344, 415, 433
CviQI GTAC 2 cut(s) 31, 37
DdeI CTNAG 3 cut(s) 140, 276, 375
DpnI GATC 4 cut(s) 162, 188, 235, 387
DpnII GATC 4 cut(s) 160, 186, 233, 385
DriI GACNNNNNGTC 1 cut(s) 53
Eam1105I GACNNNNNGTC 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 319, 454
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 128
Eco81I CCTNAGG 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 330
EcoT14I CCWWGG 2 cut(s) 319, 454
ErhI CCWWGG 2 cut(s) 319, 454
FaeI CATG 6 cut(s) 101, 323, 357, 367, 450, 474
FaiI YATR 9 cut(s) 11, 24, 99, 288, 321, 355, 365, 448, 472
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FatI CATG 6 cut(s) 97, 319, 353, 363, 446, 470
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 3 cut(s) 299, 302, 311
Fsp4HI GCNGC 3 cut(s) 299, 302, 311
FspBI CTAG 2 cut(s) 53, 102
GluI GCNGC 3 cut(s) 299, 302, 311
HaeIII GGCC 1 cut(s) 324
Hin1II CATG 6 cut(s) 101, 323, 357, 367, 450, 474
HinfI GANTC 2 cut(s) 71, 167
HphI GGTGA 1 cut(s) 124
Hpy188III TCNNGA 5 cut(s) 164, 237, 278, 428, 471
HpyAV CCTTC 3 cut(s) 100, 103, 404
HpyCH4V TGCA 2 cut(s) 130, 310
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 310
HpyF3I CTNAG 3 cut(s) 140, 276, 375
Hsp92II CATG 6 cut(s) 101, 323, 357, 367, 450, 474
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 4 cut(s) 160, 186, 233, 385
LmnI GCTCC 3 cut(s) 199, 295, 349
LpnPI CCDG 9 cut(s) 62, 70, 75, 149, 263, 317, 326, 344, 425
Lsp1109I GCAGC 3 cut(s) 310, 313, 322
LweI GCATC 1 cut(s) 449
MabI ACCWGGT 1 cut(s) 330
MaeI CTAG 2 cut(s) 53, 102
MaeIII GTNAC 1 cut(s) 80
MalI GATC 4 cut(s) 162, 188, 235, 387
MbiI CCGCTC 1 cut(s) 28
MboI GATC 4 cut(s) 160, 186, 233, 385
MboII GAAGA 2 cut(s) 170, 271
MflI RGATCY 2 cut(s) 160, 186
MhlI GDGCHC 1 cut(s) 374
MluCI AATT 3 cut(s) 214, 228, 289
MnlI CCTC 3 cut(s) 274, 396, 399
MseI TTAA 3 cut(s) 222, 246, 327
MslI CAYNNNNRTG 1 cut(s) 352
MspCI CTTAAG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 332
MvaI CCWGG 1 cut(s) 332
MwoI GCNNNNNNNGC 2 cut(s) 307, 310
NcoI CCATGG 1 cut(s) 319
NdeII GATC 4 cut(s) 160, 186, 233, 385
NlaIII CATG 6 cut(s) 101, 323, 357, 367, 450, 474
NlaIV GGNNCC 2 cut(s) 345, 410
NspI RCATGY 1 cut(s) 101
NspV TTCGAA 1 cut(s) 93
PaeR7I CTCGAG 1 cut(s) 428
PagI TCATGA 1 cut(s) 470
PfeI GAWTC 2 cut(s) 71, 167
Pfl23II CGTACG 1 cut(s) 36
PflMI CCANNNNNTGG 1 cut(s) 196
PkrI GCNGC 3 cut(s) 300, 303, 312
Psp6I CCWGG 1 cut(s) 330
PspGI CCWGG 1 cut(s) 330
PspLI CGTACG 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 345, 410
PspPI GGNCC 1 cut(s) 87
PstI CTGCAG 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 2 cut(s) 160, 186
RsaI GTAC 2 cut(s) 32, 38
RsaNI GTAC 2 cut(s) 31, 37
RseI CAYNNNNRTG 1 cut(s) 352
SaqAI TTAA 3 cut(s) 222, 246, 327
SatI GCNGC 3 cut(s) 299, 302, 311
Sau3AI GATC 4 cut(s) 160, 186, 233, 385
Sau96I GGNCC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 332
SduI GDGCHC 1 cut(s) 374
SexAI ACCWGGT 1 cut(s) 330
SfaNI GCATC 1 cut(s) 449
SfcI CTRYAG 1 cut(s) 308
Sfr274I CTCGAG 1 cut(s) 428
SfuI TTCGAA 1 cut(s) 93
SinI GGWCC 1 cut(s) 87
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 352
SmlI CTYRAG 2 cut(s) 221, 428
SmoI CTYRAG 2 cut(s) 221, 428
Sse9I AATT 3 cut(s) 214, 228, 289
SsiI CCGC 2 cut(s) 28, 269
SspMI CTAG 2 cut(s) 53, 102
StyD4I CCNGG 1 cut(s) 330
StyI CCWWGG 2 cut(s) 319, 454
TaqI TCGA 5 cut(s) 57, 93, 232, 417, 429
TasI AATT 3 cut(s) 214, 228, 289
TfiI GAWTC 2 cut(s) 71, 167
Tru1I TTAA 3 cut(s) 222, 246, 327
Tru9I TTAA 3 cut(s) 222, 246, 327
TseI GCWGC 3 cut(s) 298, 301, 310
TspDTI ATGAA 4 cut(s) 159, 275, 435, 459
Van91I CCANNNNNTGG 1 cut(s) 196
Vha464I CTTAAG 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 87
XapI RAATTY 1 cut(s) 228
XceI RCATGY 1 cut(s) 101
XhoI CTCGAG 1 cut(s) 428
XspI CTAG 2 cut(s) 53, 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.