Rmu_sc0005531.1_g000002

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005531.1
OG0003525
Physical Location & Seq
Reverse (-)
2716 .. 4609
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
atgcctttgactataagcccttcttcaacatccataatctcccaaagaccaaaccttggtttcttcaaaaacactggaccagctttcattaaactcccaaagagcaacaacaatggcaacgtgggtagtggtggtttggtaatattcgcctcaaatggggctaataatcgacaagcactggaggtggtgttcgagccctttgaggaggtcaagaaggagctccttctcgtcccaactgagcctaatgcttctcttgctcgccagaaatacacagacgaaagtgtagctgccattaatgaacaaatcaagtggaacaaacgtggaggaagagttaagctacagtccatgctaatgccctgctcagagtttgatcatgctgagaagggtgatgcgttgcacgcaatggagcttgcgcttgctttggaaaagctgaacaatgagaaacttctaaatctgcacaaaatagctaatgaaaacaatgatgtgcatttagcggattacattgaaagtgaatacttgaatgaacaggtcgaatctataaggaaaatttctgaatatgtagctcagttgagaaggattggtccaggacatggtgtttggcactttgatcagatgctccttaatgacacagccggggtcgccgcatga

Protein Analysis

215

Amino Acids

23.81

Weight (kDa)

5.89

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin PF00210 105 - 193 5.7e-12 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 56, 590
AciI CCGC 2 cut(s) 494, 642
AcsI RAATTY 1 cut(s) 546
AfiI CCNNNNNNNGG 3 cut(s) 56, 156, 590
AgsI TTSAA 4 cut(s) 27, 67, 506, 520
AjnI CCWGG 1 cut(s) 583
AloI GAACNNNNNNTCC 2 cut(s) 173, 205
AluBI AGCT 8 cut(s) 83, 220, 287, 337, 409, 430, 467, 563
AluI AGCT 8 cut(s) 83, 220, 287, 337, 409, 430, 467, 563
Alw21I GWGCWC 1 cut(s) 222
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 546
ArsI GACNNNNNNTTYG 2 cut(s) 579, 611
AseI ATTAAT 1 cut(s) 294
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 2 cut(s) 77, 581
AsuC2I CCSGG 1 cut(s) 634
AsuHPI GGTGA 1 cut(s) 398
AvaII GGWCC 2 cut(s) 77, 581
BanII GRGCYC 2 cut(s) 198, 222
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 585
BclI TGATCA 2 cut(s) 370, 607
BcnI CCSGG 1 cut(s) 634
BfmI CTRYAG 1 cut(s) 338
BisI GCNGC 2 cut(s) 288, 642
BlsI GCNGC 2 cut(s) 289, 643
Bme1390I CCNGG 2 cut(s) 585, 634
Bme18I GGWCC 2 cut(s) 77, 581
BmgT120I GGNCC 2 cut(s) 77, 581
BmrFI CCNGG 2 cut(s) 585, 634
BmsI GCATC 2 cut(s) 379, 603
BpmI CTGGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 634
BsaJI CCNNGG 2 cut(s) 55, 633
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 315, 345
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 156, 590
Bse1I ACTGG 2 cut(s) 79, 183
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 1 cut(s) 585
BseDI CCNNGG 2 cut(s) 55, 633
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 3 cut(s) 56, 156, 590
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 4 cut(s) 228, 369, 375, 578
BseNI ACTGG 2 cut(s) 79, 183
BseRI GAGGAG 1 cut(s) 218
BseXI GCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 440
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 1 cut(s) 633
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 56, 156, 590
BsmFI GGGAC 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 198, 222
Bsp143I GATC 2 cut(s) 370, 607
BspACI CCGC 2 cut(s) 494, 642
BspCNI CTCAG 4 cut(s) 229, 370, 374, 577
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 2 cut(s) 79, 183
BssECI CCNNGG 2 cut(s) 55, 633
BssMI GATC 2 cut(s) 370, 607
BssT1I CCWWGG 1 cut(s) 55
Bst2UI CCWGG 1 cut(s) 585
Bst4CI ACNGT 1 cut(s) 342
Bst6I CTCTTC 1 cut(s) 322
BstC8I GCNNGC 4 cut(s) 259, 399, 411, 417
BstDEI CTNAG 4 cut(s) 237, 361, 378, 564
BstF5I GGATG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 2 cut(s) 373, 610
BstMBI GATC 2 cut(s) 370, 607
BstMWI GCNNNNNNNGC 3 cut(s) 254, 398, 638
BstNI CCWGG 1 cut(s) 585
BstSCI CCNGG 2 cut(s) 583, 632
BstSFI CTRYAG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 274
BtsCI GGATG 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 72, 176
Cac8I GCNNGC 4 cut(s) 259, 399, 411, 417
CfoI GCGC 1 cut(s) 415
Cfr13I GGNCC 2 cut(s) 77, 581
CspCI CAANNNNNGTGG 2 cut(s) 290, 325
CviAII CATG 4 cut(s) 346, 374, 590, 645
DdeI CTNAG 4 cut(s) 237, 361, 378, 564
DpnI GATC 2 cut(s) 372, 609
DpnII GATC 2 cut(s) 370, 607
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
Ecl136II GAGCTC 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 55
Eco24I GRGCYC 2 cut(s) 198, 222
Eco47I GGWCC 2 cut(s) 77, 581
Eco53kI GAGCTC 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 583
EcoT14I CCWWGG 1 cut(s) 55
EcoT38I GRGCYC 2 cut(s) 198, 222
ErhI CCWWGG 1 cut(s) 55
FaeI CATG 4 cut(s) 349, 377, 593, 648
FaiI YATR 8 cut(s) 14, 35, 347, 375, 539, 558, 591, 646
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FaqI GGGAC 1 cut(s) 215
FatI CATG 4 cut(s) 345, 373, 589, 644
FbaI TGATCA 2 cut(s) 370, 607
Fnu4HI GCNGC 2 cut(s) 288, 642
FokI GGATG 1 cut(s) 16
FriOI GRGCYC 2 cut(s) 198, 222
Fsp4HI GCNGC 2 cut(s) 288, 642
GlaI GCGC 1 cut(s) 414
GluI GCNGC 2 cut(s) 288, 642
GsuI CTGGAG 1 cut(s) 200
HapII CCGG 1 cut(s) 633
HhaI GCGC 1 cut(s) 415
Hin1II CATG 4 cut(s) 349, 377, 593, 648
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HinfI GANTC 1 cut(s) 533
HpaII CCGG 1 cut(s) 633
HphI GGTGA 1 cut(s) 398
Hpy188I TCNGA 3 cut(s) 364, 553, 612
Hpy188III TCNNGA 1 cut(s) 211
HpyAV CCTTC 5 cut(s) 30, 208, 233, 376, 567
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 2 cut(s) 120, 319
HpyCH4V TGCA 3 cut(s) 397, 457, 487
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 398, 638
HpyF3I CTNAG 4 cut(s) 237, 361, 378, 564
HpySE526I ACGT 2 cut(s) 120, 319
Hsp92II CATG 4 cut(s) 349, 377, 593, 648
HspAI GCGC 1 cut(s) 413
Ksp22I TGATCA 2 cut(s) 370, 607
Kzo9I GATC 2 cut(s) 370, 607
LmnI GCTCC 4 cut(s) 217, 225, 406, 621
LpnPI CCDG 8 cut(s) 60, 93, 164, 275, 370, 512, 570, 597
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 2 cut(s) 379, 603
MaeII ACGT 2 cut(s) 120, 319
MalI GATC 2 cut(s) 372, 609
MboI GATC 2 cut(s) 370, 607
MboII GAAGA 3 cut(s) 15, 55, 339
MhlI GDGCHC 2 cut(s) 198, 222
MluCI AATT 1 cut(s) 546
MnlI CCTC 5 cut(s) 160, 175, 196, 199, 317
MseI TTAA 4 cut(s) 90, 294, 333, 621
MslI CAYNNNNRTG 1 cut(s) 350
MspI CCGG 1 cut(s) 633
MspR9I CCNGG 2 cut(s) 585, 634
MvaI CCWGG 1 cut(s) 585
MwoI GCNNNNNNNGC 3 cut(s) 254, 398, 638
NciI CCSGG 1 cut(s) 634
NdeII GATC 2 cut(s) 370, 607
NlaIII CATG 4 cut(s) 349, 377, 593, 648
PfeI GAWTC 1 cut(s) 533
PflMI CCANNNNNTGG 2 cut(s) 56, 590
PfoI TCCNGGA 1 cut(s) 583
PkrI GCNGC 2 cut(s) 289, 643
PshBI ATTAAT 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 583
PspGI CCWGG 1 cut(s) 583
PspPI GGNCC 2 cut(s) 77, 581
RseI CAYNNNNRTG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 4 cut(s) 90, 294, 333, 621
SatI GCNGC 2 cut(s) 288, 642
Sau3AI GATC 2 cut(s) 370, 607
Sau96I GGNCC 2 cut(s) 77, 581
ScrFI CCNGG 2 cut(s) 585, 634
SduI GDGCHC 2 cut(s) 198, 222
SfaNI GCATC 2 cut(s) 379, 603
SfcI CTRYAG 1 cut(s) 338
SinI GGWCC 2 cut(s) 77, 581
SmiMI CAYNNNNRTG 1 cut(s) 350
Sse9I AATT 1 cut(s) 546
SsiI CCGC 2 cut(s) 494, 642
SspI AATATT 1 cut(s) 144
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 583, 632
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 2 cut(s) 123, 322
TaqI TCGA 3 cut(s) 169, 192, 531
TasI AATT 1 cut(s) 546
TauI GCSGC 1 cut(s) 644
TfiI GAWTC 1 cut(s) 533
Tru1I TTAA 4 cut(s) 90, 294, 333, 621
Tru9I TTAA 4 cut(s) 90, 294, 333, 621
TscAI CASTG 2 cut(s) 79, 183
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 4 cut(s) 76, 312, 486, 537
TspRI CASTG 2 cut(s) 79, 183
Van91I CCANNNNNTGG 2 cut(s) 56, 590
VpaK11BI GGWCC 2 cut(s) 77, 581
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.