Rmu_sc0006137.1_g000001

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006137.1
OG0002602
Physical Location & Seq
Reverse (-)
1 .. 1318
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 593 bp
atggtgacgatgacgaagaagacgtcgccaccgccaatcacggcgcgccacgtgattaggtccagctggaagcttctgatcgtcttctccgtcttgctctgcgttttggctctgtacaggtttcagtctcagcagccagacctctactctccttcttcttcttcttcttcttcattatctcgcgcgagatctcgaattgctcgccacagcgtcagcttcgccggccccgccaagatcgccttcctatttctcgcgcgacgtgacctcccgctcgattttctttgggagagcttcttcgagaatgccggtggcgctttgaatttttcgatttatattcactcggcgcctggttttgtgttcgatgagtccacgacgaggtctcgcttctttcacggccgtcagttgccgaatagcatacaggtgggatggggagaatcgagcatgattgaagcggagaggttgttgtttgcaacagctcttgaggatccggcaaatcaaagatttgttctcctctctgacagttgcgttcctttgtacaacttcagcttcatatacaattacctgatggcttctcctaggagtattgtggacag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

197

Amino Acids

22.2

Weight (kDa)

9.51

Isoelectric Point (pI)

58.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 79 - 195 1e-33 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 343
AccBSI CCGCTC 1 cut(s) 271
AccII CGCG 5 cut(s) 46, 183, 185, 254, 256
AciI CCGC 4 cut(s) 32, 228, 269, 452
AclWI GGATC 2 cut(s) 479, 492
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 526
AcvI CACGTG 1 cut(s) 52
AcyI GRCGYC 2 cut(s) 23, 344
AfaI GTAC 2 cut(s) 116, 536
AfiI CCNNNNNNNGG 1 cut(s) 375
AgsI TTSAA 2 cut(s) 319, 449
AjiI CACGTC 1 cut(s) 260
AjnI CCWGG 1 cut(s) 346
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 6 cut(s) 66, 73, 216, 291, 476, 546
AluI AGCT 6 cut(s) 66, 73, 216, 291, 476, 546
Alw26I GTCTC 2 cut(s) 132, 384
AlwI GGATC 2 cut(s) 479, 492
AoxI GGCC 2 cut(s) 223, 394
ApeKI GCWGC 1 cut(s) 133
ApoI RAATTY 1 cut(s) 319
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AscI GGCGCGCC 1 cut(s) 44
AspA2I CCTAGG 1 cut(s) 575
AspLEI GCGC 6 cut(s) 46, 48, 185, 256, 314, 346
AspS9I GGNCC 2 cut(s) 60, 224
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 60
AvrII CCTAGG 1 cut(s) 575
BamHI GGATCC 1 cut(s) 484
BanI GGYRCC 1 cut(s) 343
BbrPI CACGTG 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 26, 76
BbvI GCAGC 1 cut(s) 145
BccI CCATC 2 cut(s) 420, 559
BceAI ACGGC 3 cut(s) 57, 381, 409
BciT130I CCWGG 1 cut(s) 348
BcoDI GTCTC 2 cut(s) 132, 384
BfaI CTAG 1 cut(s) 576
BfoI RGCGCY 2 cut(s) 315, 347
BglII AGATCT 1 cut(s) 188
BisI GCNGC 1 cut(s) 134
BlnI CCTAGG 1 cut(s) 575
BlsI GCNGC 1 cut(s) 135
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 60, 224
BmiI GGNNCC 3 cut(s) 226, 345, 486
BmrFI CCNGG 1 cut(s) 348
BpiI GAAGAC 2 cut(s) 26, 76
BpuEI CTTGAG 1 cut(s) 500
BsaAI YACGTR 1 cut(s) 52
BsaHI GRCGYC 2 cut(s) 23, 344
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 575
BsaXI ACNNNNNCTCC 4 cut(s) 249, 279, 446, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse118I RCCGGY 2 cut(s) 221, 305
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 575
BseGI GGATG 1 cut(s) 431
BseLI CCNNNNNNNGG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 143
BsePI GCGCGC 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 500
BseX3I CGGCCG 1 cut(s) 394
BseXI GCAGC 1 cut(s) 145
Bsh1236I CGCG 5 cut(s) 46, 183, 185, 254, 256
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 2 cut(s) 225, 396
BshNI GGYRCC 1 cut(s) 343
BsiEI CGRYCG 1 cut(s) 397
BsiSI CCGG 3 cut(s) 222, 306, 488
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 2 cut(s) 132, 384
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 2 cut(s) 225, 396
Bso31I GGTCTC 1 cut(s) 384
Bsp1407I TGTACA 2 cut(s) 114, 534
Bsp143I GATC 4 cut(s) 78, 188, 234, 484
BspACI CCGC 4 cut(s) 32, 228, 269, 452
BspANI GGCC 2 cut(s) 225, 396
BspCNI CTCAG 1 cut(s) 142
BspFNI CGCG 5 cut(s) 46, 183, 185, 254, 256
BspLI GGNNCC 3 cut(s) 226, 345, 486
BspPI GGATC 2 cut(s) 479, 492
BspT107I GGYRCC 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 384
BsrBI CCGCTC 1 cut(s) 271
BsrFI RCCGGY 2 cut(s) 221, 305
BsrGI TGTACA 2 cut(s) 114, 534
BssAI RCCGGY 2 cut(s) 221, 305
BssECI CCNNGG 1 cut(s) 575
BssHII GCGCGC 1 cut(s) 44
BssMI GATC 4 cut(s) 78, 188, 234, 484
BssNI GRCGYC 2 cut(s) 23, 344
BssT1I CCWWGG 1 cut(s) 575
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 521
BstACI GRCGYC 2 cut(s) 23, 344
BstAUI TGTACA 2 cut(s) 114, 534
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 3 cut(s) 46, 202, 223
BstDEI CTNAG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 431
BstFNI CGCG 5 cut(s) 46, 183, 185, 254, 256
BstH2I RGCGCY 2 cut(s) 315, 347
BstHHI GCGC 6 cut(s) 46, 48, 185, 256, 314, 346
BstKTI GATC 4 cut(s) 81, 191, 237, 487
BstMAI GTCTC 2 cut(s) 132, 384
BstMBI GATC 4 cut(s) 78, 188, 234, 484
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 4 cut(s) 222, 227, 236, 311
BstNI CCWGG 1 cut(s) 348
BstSCI CCNGG 1 cut(s) 346
BstUI CGCG 5 cut(s) 46, 183, 185, 254, 256
BstV1I GCAGC 1 cut(s) 145
BstV2I GAAGAC 2 cut(s) 26, 76
BstX2I RGATCY 2 cut(s) 188, 484
BstYI RGATCY 2 cut(s) 188, 484
BstZI CGGCCG 1 cut(s) 394
BsuRI GGCC 2 cut(s) 225, 396
BtrI CACGTC 1 cut(s) 260
BtsCI GGATG 1 cut(s) 431
Cac8I GCNNGC 3 cut(s) 46, 202, 223
CfoI GCGC 6 cut(s) 46, 48, 185, 256, 314, 346
Cfr10I RCCGGY 2 cut(s) 221, 305
Cfr13I GGNCC 2 cut(s) 60, 224
CseI GACGC 1 cut(s) 199
Csp6I GTAC 2 cut(s) 115, 535
CviAII CATG 1 cut(s) 442
CviQI GTAC 2 cut(s) 115, 535
DdeI CTNAG 1 cut(s) 129
DinI GGCGCC 1 cut(s) 345
DpnI GATC 4 cut(s) 80, 190, 236, 486
DpnII GATC 4 cut(s) 78, 188, 234, 484
EaeI YGGCCR 1 cut(s) 394
EagI CGGCCG 1 cut(s) 394
EclXI CGGCCG 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 575
Eco31I GGTCTC 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 60
Eco52I CGGCCG 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 526
Eco72I CACGTG 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 575
EgeI GGCGCC 1 cut(s) 345
EheI GGCGCC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 575
FaeI CATG 1 cut(s) 445
FaiI YATR 5 cut(s) 333, 416, 443, 551, 553
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FatI CATG 1 cut(s) 441
FauI CCCGC 2 cut(s) 235, 276
Fnu4HI GCNGC 1 cut(s) 134
FokI GGATG 1 cut(s) 438
Fsp4HI GCNGC 1 cut(s) 134
FspBI CTAG 1 cut(s) 576
GlaI GCGC 6 cut(s) 45, 47, 184, 255, 313, 345
GluI GCNGC 1 cut(s) 134
HaeII RGCGCY 2 cut(s) 315, 347
HaeIII GGCC 2 cut(s) 225, 396
HapII CCGG 3 cut(s) 222, 306, 488
HgaI GACGC 1 cut(s) 199
HhaI GCGC 6 cut(s) 46, 48, 185, 256, 314, 346
Hin1I GRCGYC 2 cut(s) 23, 344
Hin1II CATG 1 cut(s) 445
Hin6I GCGC 6 cut(s) 44, 46, 183, 254, 312, 344
HinP1I GCGC 6 cut(s) 44, 46, 183, 254, 312, 344
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 365, 434
HpaII CCGG 3 cut(s) 222, 306, 488
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 369, 589
Hpy188I TCNGA 2 cut(s) 78, 517
Hpy188III TCNNGA 3 cut(s) 192, 298, 479
Hpy8I GTNNAC 2 cut(s) 369, 589
Hpy99I CGWCG 3 cut(s) 28, 261, 376
HpyAV CCTTC 2 cut(s) 162, 250
HpyCH4III ACNGT 1 cut(s) 521
HpyCH4IV ACGT 3 cut(s) 23, 51, 259
HpyCH4V TGCA 1 cut(s) 470
HpyF10VI GCNNNNNNNGC 4 cut(s) 222, 227, 236, 311
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 3 cut(s) 23, 51, 259
Hsp92I GRCGYC 2 cut(s) 23, 344
Hsp92II CATG 1 cut(s) 445
HspAI GCGC 6 cut(s) 44, 46, 183, 254, 312, 344
KasI GGCGCC 1 cut(s) 343
KroI GCCGGC 1 cut(s) 221
KroNI GCCGGC 1 cut(s) 223
Kzo9I GATC 4 cut(s) 78, 188, 234, 484
Lsp1109I GCAGC 1 cut(s) 145
MaeI CTAG 1 cut(s) 576
MaeII ACGT 3 cut(s) 23, 51, 259
MaeIII GTNAC 2 cut(s) 4, 260
MalI GATC 4 cut(s) 80, 190, 236, 486
MbiI CCGCTC 1 cut(s) 271
MboI GATC 4 cut(s) 78, 188, 234, 484
MflI RGATCY 2 cut(s) 188, 484
MluCI AATT 3 cut(s) 195, 319, 556
Mly113I GGCGCC 1 cut(s) 344
MlyI GAGTC 1 cut(s) 374
MnlI CCTC 6 cut(s) 152, 275, 369, 450, 475, 521
MroNI GCCGGC 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 3 cut(s) 222, 306, 488
MspR9I CCNGG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 1 cut(s) 348
MvnI CGCG 5 cut(s) 46, 183, 185, 254, 256
MwoI GCNNNNNNNGC 4 cut(s) 222, 227, 236, 311
NaeI GCCGGC 1 cut(s) 223
NarI GGCGCC 1 cut(s) 344
NdeII GATC 4 cut(s) 78, 188, 234, 484
NgoMIV GCCGGC 1 cut(s) 221
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 3 cut(s) 226, 345, 486
NmeAIII GCCGAG 1 cut(s) 320
NmuCI GTSAC 2 cut(s) 4, 260
PalAI GGCGCGCC 1 cut(s) 44
PauI GCGCGC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 87
PctI GAATGC 1 cut(s) 307
PdiI GCCGGC 1 cut(s) 223
PfeI GAWTC 1 cut(s) 434
PflFI GACNNNGTC 1 cut(s) 376
PkrI GCNGC 1 cut(s) 135
PleI GAGTC 1 cut(s) 373
PluTI GGCGCC 1 cut(s) 347
PmaCI CACGTG 1 cut(s) 52
PmlI CACGTG 1 cut(s) 52
PpsI GAGTC 1 cut(s) 373
Ppu21I YACGTR 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 346
PspCI CACGTG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 3 cut(s) 226, 345, 486
PspPI GGNCC 2 cut(s) 60, 224
PsuI RGATCY 2 cut(s) 188, 484
PsyI GACNNNGTC 1 cut(s) 376
PteI GCGCGC 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 66
RsaI GTAC 2 cut(s) 116, 536
RsaNI GTAC 2 cut(s) 115, 535
RseI CAYNNNNRTG 1 cut(s) 419
SatI GCNGC 1 cut(s) 134
Sau3AI GATC 4 cut(s) 78, 188, 234, 484
Sau96I GGNCC 2 cut(s) 60, 224
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 1 cut(s) 348
SfoI GGCGCC 1 cut(s) 345
SgsI GGCGCGCC 1 cut(s) 44
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 3 cut(s) 195, 319, 556
SsiI CCGC 4 cut(s) 32, 228, 269, 452
SspDI GGCGCC 1 cut(s) 343
SspMI CTAG 1 cut(s) 576
StyD4I CCNGG 1 cut(s) 346
StyI CCWWGG 1 cut(s) 575
TaaI ACNGT 1 cut(s) 521
TaiI ACGT 3 cut(s) 26, 54, 262
TaqI TCGA 6 cut(s) 193, 273, 297, 326, 360, 437
TasI AATT 3 cut(s) 195, 319, 556
TatI WGTACW 2 cut(s) 114, 534
TfiI GAWTC 1 cut(s) 434
TseFI GTSAC 2 cut(s) 4, 260
TseI GCWGC 1 cut(s) 133
Tsp45I GTSAC 2 cut(s) 4, 260
TspDTI ATGAA 2 cut(s) 162, 538
TspGWI ACGGA 1 cut(s) 79
Tth111I GACNNNGTC 1 cut(s) 376
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 1 cut(s) 319
XmaJI CCTAGG 1 cut(s) 575
XspI CTAG 1 cut(s) 576
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.