Rmu_sc0006432.1_g000008

Patellin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006432.1
OG0003961
Physical Location & Seq
Forward (+)
25697 .. 33954
8258 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1353 bp
atgcatgaattccaggacgaggatgattggtatgtggatcatgggtgttgttgggagggtacccaaaacgatagctcccgtgatggtgatcatgtaactgttgctttttcgaggtttactcaagtatactattgcttagtatgcttgaaggcctgggctagtgcctcattgcttccaattcttgacacggtgacaccggatgacaggagtgatgtgattcttctcaagtttctaagggctcgggacttcaaggtgaaagatgcttttgttatgcttcgaaacacgatccactggaggaagcagtttaagattgatgctcttgcggaggaagatctcggtgatgaattagaaaaagtggtgtttatgcacgggtatgatagagaggttatggagaggcctctgctcgaggcgatggaggagtcatttgcagcggagatggtgaaatacaccatcagaatggcggtaacaagtgtcgccctactggttttagggcttcaattcgagaaagaaaggcgttcaatttttgttcttactgttcttgaatgtggaatcgctattttgggtattgggatttgtgggttttgtgtggtggtgacggtagggatctgggggattagaaatgagaagttgagtggtagtggcttggagaagatgaataggtatggttatggtggagagctcggtggtggttatgggtcgtctcctgatcaatgtccagaggggacattgtttatgttggatagtgctagtcctgatctttgtgttgagttgatatggtcgttgtacatatttcctaagagacagatttggagatgttgctggtgtcttaaacccagagatgaggagtatggagcaataaataaccctaggggaaagtatacgctaccggatgatctgaagaaagtggtggaggaggggctgaaagggaagtggcatctcttggatcatgggtttgctaatgttcctttgacactgatggttaagtgttttccgaagcctgttgatgatgcttcacaagccaatgagaagctagaatggcctcaggcacaatcctcatattcagctgatgttgttaactacattagagatcttgaggcagaaaaagatatcccattgttaaaggagaatttgattgaccttatgaagtctgaaatcactctaaagatttgtacgatgcttttgaagagaggggtggaaagtggccttactccttatgaaattgggtgtatgatgatgagggacaatgagcatgacaaatcgtctctcgaaaagttgattgaagaggcgcgagaagctcacggaaatgatgattcgttgttggatcatgtattatccatgattgatggtaggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0009507 GO:0009536 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

450

Amino Acids

51.16

Weight (kDa)

4.92

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 65 - 94 3.6e-09 CRAL/TRIO, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 59
AccI GTMKAC 2 cut(s) 126, 878
AccII CGCG 1 cut(s) 1288
AciI CCGC 3 cut(s) 323, 431, 461
AclWI GGATC 5 cut(s) 45, 280, 611, 951, 1329
AcsI RAATTY 2 cut(s) 8, 1126
AcuI CTGAAG 1 cut(s) 917
AfaI GTAC 3 cut(s) 61, 785, 1171
AfiI CCNNNNNNNGG 1 cut(s) 19
AgsI TTSAA 7 cut(s) 148, 250, 497, 519, 542, 1183, 1280
AhdI GACNNNNNGTC 1 cut(s) 1258
AjnI CCWGG 2 cut(s) 12, 152
AluBI AGCT 5 cut(s) 75, 679, 1030, 1064, 1295
AluI AGCT 5 cut(s) 75, 679, 1030, 1064, 1295
Alw21I GWGCWC 1 cut(s) 681
Alw26I GTCTC 3 cut(s) 705, 793, 1266
AlwI GGATC 5 cut(s) 45, 280, 611, 951, 1329
Ama87I CYCGRG 2 cut(s) 240, 404
AoxI GGCC 4 cut(s) 150, 395, 1037, 1201
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 2 cut(s) 8, 1126
Asp718I GGTACC 1 cut(s) 59
AspA2I CCTAGG 1 cut(s) 866
AspLEI GCGC 1 cut(s) 1288
AsuHPI GGTGA 6 cut(s) 98, 202, 265, 350, 451, 604
AsuII TTCGAA 1 cut(s) 277
AvaI CYCGRG 2 cut(s) 240, 404
AvrII CCTAGG 1 cut(s) 866
AxyI CCTNAGG 1 cut(s) 1041
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 2 cut(s) 241, 681
Bbv12I GWGCWC 1 cut(s) 681
BbvI GCAGC 1 cut(s) 440
BccI CCATC 6 cut(s) 77, 406, 430, 458, 970, 1337
BciT130I CCWGG 2 cut(s) 14, 154
BclI TGATCA 2 cut(s) 88, 706
BcoDI GTCTC 3 cut(s) 705, 793, 1266
BfaI CTAG 4 cut(s) 159, 747, 867, 1031
BglII AGATCT 2 cut(s) 331, 1087
BisI GCNGC 1 cut(s) 429
BlnI CCTAGG 1 cut(s) 866
BlsI GCNGC 1 cut(s) 430
Bme1390I CCNGG 2 cut(s) 14, 154
BmeRI GACNNNNNGTC 1 cut(s) 1258
BmeT110I CYCGRG 2 cut(s) 240, 404
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 2 cut(s) 14, 154
BmsI GCATC 5 cut(s) 250, 304, 943, 997, 1164
BplI GAGNNNNNCTC 2 cut(s) 103, 135
BpmI CTGGAG 1 cut(s) 313
Bpu14I TTCGAA 1 cut(s) 277
BpuEI CTTGAG 3 cut(s) 105, 209, 1112
BsaBI GATNNNNATC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 153, 866
BsaWI WCCGGW 2 cut(s) 196, 886
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 296, 486
Bse21I CCTNAGG 1 cut(s) 1041
Bse3DI GCAATG 1 cut(s) 167
Bse8I GATNNNNATC 1 cut(s) 87
BseBI CCWGG 2 cut(s) 14, 154
BseDI CCNNGG 2 cut(s) 153, 866
BseGI GGATG 3 cut(s) 28, 205, 895
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 19
BseMI GCAATG 1 cut(s) 167
BseMII CTCAG 1 cut(s) 1055
BseNI ACTGG 2 cut(s) 296, 486
BseRI GAGGAG 3 cut(s) 431, 857, 926
BseXI GCAGC 1 cut(s) 440
Bsh1236I CGCG 1 cut(s) 1288
BshFI GGCC 4 cut(s) 152, 397, 1039, 1203
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 681
BsiHKCI CYCGRG 2 cut(s) 240, 404
BsiSI CCGG 2 cut(s) 197, 887
BslFI GGGAC 3 cut(s) 257, 736, 1253
BslI CCNNNNNNNGG 1 cut(s) 19
BsmAI GTCTC 3 cut(s) 705, 793, 1266
BsmBI CGTCTC 2 cut(s) 705, 1266
BsmFI GGGAC 3 cut(s) 257, 736, 1253
BsnI GGCC 4 cut(s) 152, 397, 1039, 1203
BsoBI CYCGRG 2 cut(s) 240, 404
Bsp119I TTCGAA 1 cut(s) 277
Bsp1286I GDGCHC 2 cut(s) 241, 681
Bsp1407I TGTACA 1 cut(s) 783
BspACI CCGC 3 cut(s) 323, 431, 461
BspANI GGCC 4 cut(s) 152, 397, 1039, 1203
BspCNI CTCAG 1 cut(s) 1054
BspFNI CGCG 1 cut(s) 1288
BspLI GGNNCC 1 cut(s) 61
BspPI GGATC 5 cut(s) 45, 280, 611, 951, 1329
BspT104I TTCGAA 1 cut(s) 277
BspT107I GGYRCC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 167
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 2 cut(s) 296, 486
BssECI CCNNGG 2 cut(s) 153, 866
BssNAI GTATAC 2 cut(s) 127, 879
BssT1I CCWWGG 1 cut(s) 866
Bst1107I GTATAC 2 cut(s) 127, 879
Bst2UI CCWGG 2 cut(s) 14, 154
Bst4CI ACNGT 4 cut(s) 100, 190, 535, 598
Bst6I CTCTTC 2 cut(s) 1178, 1275
BstAUI TGTACA 1 cut(s) 783
BstBI TTCGAA 1 cut(s) 277
BstDEI CTNAG 4 cut(s) 136, 233, 795, 1041
BstF5I GGATG 3 cut(s) 28, 205, 895
BstFNI CGCG 1 cut(s) 1288
BstHHI GCGC 1 cut(s) 1288
BstMAI GTCTC 3 cut(s) 705, 793, 1266
BstMWI GCNNNNNNNGC 4 cut(s) 141, 1016, 1036, 1292
BstNI CCWGG 2 cut(s) 14, 154
BstSCI CCNGG 2 cut(s) 12, 152
BstUI CGCG 1 cut(s) 1288
BstV1I GCAGC 1 cut(s) 440
BstX2I RGATCY 3 cut(s) 331, 603, 1087
BstXI CCANNNNNNTGG 1 cut(s) 457
BstYI RGATCY 3 cut(s) 331, 603, 1087
BstZ17I GTATAC 2 cut(s) 127, 879
Bsu36I CCTNAGG 1 cut(s) 1041
BsuRI GGCC 4 cut(s) 152, 397, 1039, 1203
BtgZI GCGATG 1 cut(s) 425
BtsCI GGATG 3 cut(s) 28, 205, 895
BtsIMutI CAGTG 2 cut(s) 289, 971
CfoI GCGC 1 cut(s) 1288
Csp6I GTAC 3 cut(s) 60, 784, 1170
CviAII CATG 7 cut(s) 5, 41, 92, 947, 1250, 1325, 1336
CviQI GTAC 3 cut(s) 60, 784, 1170
DdeI CTNAG 4 cut(s) 136, 233, 795, 1041
DriI GACNNNNNGTC 1 cut(s) 1258
Eam1104I CTCTTC 2 cut(s) 1178, 1275
Eam1105I GACNNNNNGTC 1 cut(s) 1258
EarI CTCTTC 2 cut(s) 1178, 1275
Ecl136II GAGCTC 1 cut(s) 679
Eco130I CCWWGG 1 cut(s) 866
Eco147I AGGCCT 2 cut(s) 152, 397
Eco24I GRGCYC 2 cut(s) 241, 681
Eco32I GATATC 1 cut(s) 1108
Eco53kI GAGCTC 1 cut(s) 679
Eco57I CTGAAG 1 cut(s) 917
Eco81I CCTNAGG 1 cut(s) 1041
Eco88I CYCGRG 2 cut(s) 240, 404
EcoICRI GAGCTC 1 cut(s) 679
EcoRI GAATTC 1 cut(s) 8
EcoRII CCWGG 2 cut(s) 12, 152
EcoRV GATATC 1 cut(s) 1108
EcoT14I CCWWGG 1 cut(s) 866
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 241, 681
ErhI CCWWGG 1 cut(s) 866
Esp3I CGTCTC 2 cut(s) 705, 1266
FaeI CATG 7 cut(s) 8, 44, 95, 950, 1253, 1328, 1339
FaqI GGGAC 3 cut(s) 257, 736, 1253
FatI CATG 7 cut(s) 4, 40, 91, 946, 1249, 1324, 1335
FbaI TGATCA 2 cut(s) 88, 706
FblI GTMKAC 2 cut(s) 126, 878
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 3 cut(s) 35, 212, 902
FriOI GRGCYC 2 cut(s) 241, 681
Fsp4HI GCNGC 1 cut(s) 429
FspBI CTAG 4 cut(s) 159, 747, 867, 1031
GlaI GCGC 1 cut(s) 1287
GluI GCNGC 1 cut(s) 429
GsuI CTGGAG 1 cut(s) 313
HaeIII GGCC 4 cut(s) 152, 397, 1039, 1203
HapII CCGG 2 cut(s) 197, 887
HhaI GCGC 1 cut(s) 1288
Hin1II CATG 7 cut(s) 8, 44, 95, 950, 1253, 1328, 1339
Hin6I GCGC 1 cut(s) 1286
HinP1I GCGC 1 cut(s) 1286
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HinfI GANTC 4 cut(s) 217, 419, 549, 1310
HpaI GTTAAC 1 cut(s) 1075
HpaII CCGG 2 cut(s) 197, 887
HphI GGTGA 6 cut(s) 98, 202, 265, 350, 451, 604
Hpy166II GTNNAC 4 cut(s) 117, 127, 879, 1075
Hpy188I TCNGA 4 cut(s) 455, 897, 993, 1150
Hpy188III TCNNGA 9 cut(s) 182, 242, 502, 539, 704, 716, 752, 1091, 1265
Hpy8I GTNNAC 4 cut(s) 117, 127, 879, 1075
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 4 cut(s) 100, 190, 535, 598
HpyCH4V TGCA 3 cut(s) 4, 367, 428
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 1016, 1036, 1292
HpyF3I CTNAG 4 cut(s) 136, 233, 795, 1041
Hsp92II CATG 7 cut(s) 8, 44, 95, 950, 1253, 1328, 1339
HspAI GCGC 1 cut(s) 1286
KpnI GGTACC 1 cut(s) 63
Ksp22I TGATCA 2 cut(s) 88, 706
KspAI GTTAAC 1 cut(s) 1075
LmnI GCTCC 2 cut(s) 80, 851
Lsp1109I GCAGC 1 cut(s) 440
LweI GCATC 5 cut(s) 250, 304, 943, 997, 1164
MaeI CTAG 4 cut(s) 159, 747, 867, 1031
MaeIII GTNAC 4 cut(s) 94, 190, 463, 592
MboII GAAGA 6 cut(s) 212, 341, 661, 910, 1195, 1292
MflI RGATCY 3 cut(s) 331, 603, 1087
MhlI GDGCHC 2 cut(s) 241, 681
MluCI AATT 7 cut(s) 8, 177, 344, 497, 519, 1126, 1218
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 2 cut(s) 717, 1299
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 5 cut(s) 306, 828, 981, 1074, 1118
MslI CAYNNNNRTG 3 cut(s) 372, 455, 1302
MspA1I CMGCKG 2 cut(s) 431, 1064
MspI CCGG 2 cut(s) 197, 887
MspR9I CCNGG 2 cut(s) 14, 154
MvaI CCWGG 2 cut(s) 14, 154
MvnI CGCG 1 cut(s) 1288
MwoI GCNNNNNNNGC 4 cut(s) 141, 1016, 1036, 1292
NlaIII CATG 7 cut(s) 8, 44, 95, 950, 1253, 1328, 1339
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 2 cut(s) 190, 592
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 277
PaeR7I CTCGAG 1 cut(s) 404
PceI AGGCCT 2 cut(s) 152, 397
PfeI GAWTC 3 cut(s) 217, 549, 1310
PfoI TCCNGGA 1 cut(s) 12
PkrI GCNGC 1 cut(s) 430
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 12, 152
PspGI CCWGG 2 cut(s) 12, 152
PspN4I GGNNCC 1 cut(s) 61
PspXI VCTCGAGB 1 cut(s) 404
PsuI RGATCY 3 cut(s) 331, 603, 1087
PvuII CAGCTG 1 cut(s) 1064
RsaI GTAC 3 cut(s) 61, 785, 1171
RsaNI GTAC 3 cut(s) 60, 784, 1170
RseI CAYNNNNRTG 3 cut(s) 372, 455, 1302
SacI GAGCTC 1 cut(s) 681
SaqAI TTAA 5 cut(s) 306, 828, 981, 1074, 1118
SatI GCNGC 1 cut(s) 429
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 2 cut(s) 14, 154
SduI GDGCHC 2 cut(s) 241, 681
SfaNI GCATC 5 cut(s) 250, 304, 943, 997, 1164
Sfr274I CTCGAG 1 cut(s) 404
SfuI TTCGAA 1 cut(s) 277
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 3 cut(s) 372, 455, 1302
SmlI CTYRAG 4 cut(s) 120, 224, 404, 1091
SmoI CTYRAG 4 cut(s) 120, 224, 404, 1091
Sse9I AATT 7 cut(s) 8, 177, 344, 497, 519, 1126, 1218
SseBI AGGCCT 2 cut(s) 152, 397
SsiI CCGC 3 cut(s) 323, 431, 461
SspMI CTAG 4 cut(s) 159, 747, 867, 1031
SstI GAGCTC 1 cut(s) 681
StuI AGGCCT 2 cut(s) 152, 397
StyD4I CCNGG 2 cut(s) 12, 152
StyI CCWWGG 1 cut(s) 866
TaaI ACNGT 4 cut(s) 100, 190, 535, 598
TaqI TCGA 5 cut(s) 110, 277, 405, 501, 1266
TasI AATT 7 cut(s) 8, 177, 344, 497, 519, 1126, 1218
TatI WGTACW 1 cut(s) 783
TfiI GAWTC 3 cut(s) 217, 549, 1310
Tru1I TTAA 5 cut(s) 306, 828, 981, 1074, 1118
Tru9I TTAA 5 cut(s) 306, 828, 981, 1074, 1118
TscAI CASTG 2 cut(s) 296, 978
TseFI GTSAC 2 cut(s) 190, 592
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 2 cut(s) 190, 592
TspDTI ATGAA 5 cut(s) 21, 357, 668, 1157, 1230
TspGWI ACGGA 1 cut(s) 1314
TspRI CASTG 2 cut(s) 296, 978
XapI RAATTY 2 cut(s) 8, 1126
XhoI CTCGAG 1 cut(s) 404
XmaJI CCTAGG 1 cut(s) 866
XmiI GTMKAC 2 cut(s) 126, 878
XspI CTAG 4 cut(s) 159, 747, 867, 1031
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.