Rmu_sc0006993.1_g000005

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006993.1
OG0002244
Physical Location & Seq
Forward (+)
25836 .. 40720
14885 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4212 bp
atggctcctagaaagaaacccaattcttcttcttccactaccgcttcgtctcggaaatcgaactcgaaagctcagcctcagccttccaagttcagcatccaacatttctttgagcgccacacccagaactctcaaaacccccaaacgaccacctcttccactcctcacaaccctaagcccccaatccccgactccgccaccgccattgttccgcagaagtcgattccggagatccaaaaccctgcgccgattgaagcaaaaagtgcactattagcttctggaaatcccgagaatcggcgtgattcgcagtcgagtagtaagaacagtacgcaggagacaccaactgagaatttggcggcggtgaggtttatggatgcggagccgaatgtggatgaggaggtctcaccggaggtttcgaagtctaagtctgtcaagcgattcaagttctcccctggaatgttgataaagcagagtcaagatgaaggaggtgatgaggtgacgtggaggacgtctccggtcaatgaaagacttaaagcagtatcaaagcacaagcctgagatgatgagagtgttggcagactcttcaaggctgaaatctttaagcattaaccaatgctcgcaaaacaagacttctgctgttgaagctggaaaggtagagaaatccctgtcgttggggtccctgaatagggttggcgtgaagagggttaaaccggatcaggatgcggaacataatggaaatgtggtcgataggactatttctgaaatagctggtagtcagagtccattccgaactccaccttctttatcatactgtcatgataagcttcctaatgctctttcatgtaatggagtttctgatgagccagggttgaggccgcataaaaaggcattgcttgaactcttagatcaagtagaagatgttatttttgttgaagattcagtacccgatgatgtggaggcatctcaattacaagttaaagataaatcagagcatgttgtaaaaagagtggtgacagctccaacagaactggttactcaggcatcttccaattacaatttccttgtattggaggtatctgagaattgtggacctcatgattcctctggtgctcagtgcatgtataaggttcttcgcttattgaatgagcaaagtggagaagagcgtgctgtgtatttacgggaagagtggttttacagtgtcattgcacctggagatactgtcaacgttattggtgaatttgatgaccaaggacagtgtcatgtggatcatcaccataactttttaattgttcaccccgatgtcttagtatctggaactcgggttgcagctaatttcatttgtcctaggcgaactgtgctggatgaaaggctaaaaggcaatgagtattcaactccagcactaattggcacaatattgcatcaagtttttgaggagaaacccacaattactttcttggaggaacatgcaagaatggggctccagagaaacattgagaacttgtatgcatgtggagttcatgaaaatgatgtatacaaaacgttggttgatgctgtcccaaaaatattaaattgggtgaacatcttcaaacacacccagaattctgaagcttcatctgttgattttggatctgagaatggggtgaaaaaggtcacaatatctgaggtaattgatattgaggagatggcatgggctccaaaatatggtctaaaaggtatgattgatgcttctgtcagggtgaaagtcgaagctaacaaaaatgaatctgacgtgaaggttatgcctttggagtttaaaaccgggaaagtgccgaaggaccaggagcattgtgcccaagtaatcttatacactcttcttatgtcggagaggtaccagaagcatgttgatactggtcttctatgttacctccagtctgatcagacacagggaattgcggttagaagatctgaccttgttgggctaatcatgcaacgtaatcaacttgcaaatgatattgtcaaggcctcaagactgcaagtccttcccccaatgttacggagtccaagcttgtgcagaagttgtcgccatctgaatgtgtgcaccatctatcacaaggcacatggaggcaacacagatagcagcggattgggagatttatttgattcaaatactcaccagctaacaactgctcatggtgttttcctaaggctttgggactggttgattgatttagaagctaaagagatggagcttgtaaagaaagaaatgtggcggccacatggtttgaagagtggtcattccacaagttgcctttcttctatggttcttgacaatgaacttccatattcaaggtctggaagtgataacaaattcatttaccgttttctgcgtgctggtttgtcttctctcaatgtgaggacatccaatggagattttcagaatgctgcttcttcaccctcaaaatatgcagattgcacactaagatgtggtgaccatgtgataattagcagtgaatctggtcatctagctgttgcaaggggggtcataacagatataagtcactcccatgtttctgtttctttttctaagaagttacgacttcctgggggcaatccttcttcagaggcaagtgatctttatcgggaggtctggcgggttgacaaagatgaatttctgacatcatttgccacaatgaggttcaatcttcttcaactttttcttccgggtgcccaaactgctcatctgagaaaaatgatcgttgacctagaggctcctaggtttgacagtggatgtattacgttcagccaggatccagcaatatcctatgtctggtcccaaagaaatttaaatgatgatcagcgtcgtgccattctaaagatacttacagcgaaggactacgctcttattctaggaatgcctggaacaggcaagacatctactatggtgcatgctgtgaaagccttgttgatgcgaggtgcatcaattttgcttacctcctacacaaattctgctgttgataatctacttatcaaattaaaagctcagaatatagactttgtgcgcattggaagacaggaagttgtgcatgaagaagttcgagggcattgcctttcagcaatgaacatacaaagtgtggaagacattaagtcaagattggaccaagtcaaggtggttgctgttacatgcttggggatcactagtcccttgcttgccaacaagaaatttgatgtatgcatcatggatgaagctggacagaccacccttccagtatctctcgggcctttgatgtttgcttcaatatttgtcctggttggtgatcattatcaattacctcctctggtgaagagtacagaggcccgagaaaatggaatgggggtaagcttgttttgcaggctctcagaagcgcatcctcaggcaatttcagcattgcaaagccagtaccgtatgtgtcaaggcattatggaactgtcaaatgccttgatatatggtgacagattgcgttgtggatctcctgaaatagccaatgccaaactcaacttttcaagttcgaagtctcattcattgtggataaaggaggttttaaatccaagcaaaccagttatatttattaatacagatgctttacctgctttcgaggcaaaggaccacaagattgtgaataacccgatggaagctcacatagttgtagaggtcgtggaggaattagttaacagtggaattgaaggggatgatattggcatcattaccccgtataactcccaggcagagatcattcggcttgccatcaaccttacttcgggaagagataaagattgcatactagtctcctttgttaggtcaactgcaaatccacgaaactgctcgtcctctctgctcggagactggcataggattaatgtggctctaactcgtgccaaggaccacaagattgtgaataacccgatggaagctcacatagttgtagaggtcgtggaggaattagttaacagtggaattgaaggggatgatattggcatcattaccccgtataactcccaggcagagatcattcggcttgccatcaaccttacttcggtggagatacataccattgacaaataccagggaagagataaagattgcatactagtctcctttgttaggtcaactgcaaatccacgaaactgctcgtcctctctgctcggagactggcataggattaatgtggctctaactcgtgccaaggtaagactttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

1403

Amino Acids

155.35

Weight (kDa)

6.66

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 382 - 579 1.7e-59 DNA replication factor Dna2
AAA_11 PF13086 940 - 1032 7e-17 AAA domain
AAA_30 PF13604 941 - 1104 2.8e-09 AAA domain
AAA_19 PF13245 945 - 1104 5.4e-07 AAA domain
AAA_11 PF13086 1041 - 1108 7e-16 AAA domain
AAA_12 PF13087 1117 - 1303 8.3e-40 AAA domain
AAA_12 PF13087 1302 - 1402 1.5e-29 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 512
Acc16I TGCGCA 1 cut(s) 3038
Acc36I ACCTGC 1 cut(s) 3610
Acc65I GGTACC 1 cut(s) 1866
AccB1I GGYRCC 2 cut(s) 1866, 2702
AccB7I CCANNNNNTGG 1 cut(s) 1700
AccI GTMKAC 1 cut(s) 1530
AccIII TCCGGA 1 cut(s) 226
AclI AACGTT 2 cut(s) 1224, 1538
AclWI GGATC 8 cut(s) 226, 720, 1272, 1633, 2780, 2793, 3176, 3490
AcoI YGGCCR 1 cut(s) 2249
AcsI RAATTY 8 cut(s) 349, 1235, 1597, 2345, 2645, 2818, 2980, 3197
AcuI CTGAAG 2 cut(s) 1623, 2580
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 5 cut(s) 328, 942, 1868, 3325, 3416
AhdI GACNNNNNGTC 2 cut(s) 2012, 3121
AhlI ACTAGT 3 cut(s) 3173, 3796, 4102
AjiI CACGTC 2 cut(s) 501, 1768
AjuI GAANNNNNNNTTGG 2 cut(s) 1040, 1072
Alw21I GWGCWC 3 cut(s) 268, 1111, 2078
Alw26I GTCTC 9 cut(s) 54, 329, 406, 516, 3534, 3805, 3849, 4111, 4155
Alw44I GTGCAC 2 cut(s) 264, 2074
AlwI GGATC 8 cut(s) 226, 720, 1272, 1633, 2780, 2793, 3176, 3490
Ama87I CYCGRG 4 cut(s) 287, 1317, 3251, 3333
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 5 cut(s) 872, 1998, 2249, 3254, 3330
ApaLI GTGCAC 2 cut(s) 264, 2074
ApeKI GCWGC 3 cut(s) 1325, 2115, 2420
ApoI RAATTY 8 cut(s) 349, 1235, 1597, 2345, 2645, 2818, 2980, 3197
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AseI ATTAAT 3 cut(s) 3585, 3870, 4176
Asp700I GAANNNNTTC 2 cut(s) 1580, 3069
Asp718I GGTACC 1 cut(s) 1866
AspA2I CCTAGG 2 cut(s) 1343, 2750
AspLEI GCGC 4 cut(s) 117, 247, 3039, 3382
AspS9I GGNCC 9 cut(s) 675, 1088, 1813, 2808, 3133, 3254, 3331, 3619, 3895
AsuC2I CCSGG 2 cut(s) 1798, 2700
AsuII TTCGAA 2 cut(s) 416, 3524
AvaI CYCGRG 4 cut(s) 287, 1317, 3251, 3333
AvaII GGWCC 7 cut(s) 675, 1088, 1813, 2808, 3133, 3619, 3895
AvrII CCTAGG 2 cut(s) 1343, 2750
AxyI CCTNAGG 2 cut(s) 2180, 3387
BaeGI GKGCMC 4 cut(s) 268, 1831, 2078, 2707
BamHI GGATCC 1 cut(s) 2785
BanI GGYRCC 2 cut(s) 1866, 2702
BanII GRGCYC 2 cut(s) 1479, 1693
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BauI CACGAG 2 cut(s) 3885, 4191
BbsI GAAGAC 4 cut(s) 1883, 2370, 3052, 3120
Bbv12I GWGCWC 3 cut(s) 268, 1111, 2078
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 1337, 2127, 2407
BccI CCATC 8 cut(s) 1675, 2070, 2087, 2215, 3637, 3767, 3913, 4043
BcgI CGANNNNNNTGC 2 cut(s) 3063, 3097
BclI TGATCA 3 cut(s) 1912, 2830, 3292
BcnI CCSGG 2 cut(s) 1798, 2700
BcoDI GTCTC 9 cut(s) 54, 329, 406, 516, 3534, 3805, 3849, 4111, 4155
BcuI ACTAGT 3 cut(s) 3173, 3796, 4102
BfaI CTAG 9 cut(s) 9, 1344, 2501, 2741, 2751, 2885, 3174, 3797, 4103
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 3610
BglII AGATCT 1 cut(s) 1940
BisI GCNGC 6 cut(s) 357, 875, 1326, 2116, 2249, 2421
BlnI CCTAGG 2 cut(s) 1343, 2750
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 6 cut(s) 358, 876, 1327, 2117, 2250, 2422
Bme18I GGWCC 7 cut(s) 675, 1088, 1813, 2808, 3133, 3619, 3895
BmeRI GACNNNNNGTC 2 cut(s) 2012, 3121
BmeT110I CYCGRG 4 cut(s) 287, 1317, 3251, 3333
BmgBI CACGTC 2 cut(s) 501, 1768
BmgT120I GGNCC 9 cut(s) 675, 1088, 1813, 2808, 3133, 3254, 3331, 3619, 3895
BpiI GAAGAC 4 cut(s) 1883, 2370, 3052, 3120
BplI GAGNNNNNCTC 4 cut(s) 386, 418, 496, 528
BpmI CTGGAG 4 cut(s) 1230, 1377, 1463, 1889
Bpu10I CCTNAGC 2 cut(s) 78, 174
Bpu1102I GCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 2 cut(s) 416, 3524
BpuEI CTTGAG 1 cut(s) 1987
BpuMI CCSGG 2 cut(s) 1798, 2700
BsaBI GATNNNNATC 2 cut(s) 2613, 3297
BsaHI GRCGYC 1 cut(s) 509
BsaI GGTCTC 1 cut(s) 406
BsaWI WCCGGW 4 cut(s) 226, 406, 514, 709
BsaXI ACNNNNNCTCC 2 cut(s) 1203, 1233
Bse1I ACTGG 9 cut(s) 1032, 1891, 1906, 2198, 3242, 3412, 3572, 3863, 4169
Bse21I CCTNAGG 2 cut(s) 2180, 3387
Bse3DI GCAATG 6 cut(s) 887, 1200, 1384, 3079, 3099, 3401
Bse8I GATNNNNATC 2 cut(s) 2613, 3297
BseAI TCCGGA 1 cut(s) 226
BseJI GATNNNNATC 2 cut(s) 2613, 3297
BseMI GCAATG 6 cut(s) 887, 1200, 1384, 3079, 3099, 3401
BseNI ACTGG 9 cut(s) 1032, 1891, 1906, 2198, 3242, 3412, 3572, 3863, 4169
BseRI GAGGAG 5 cut(s) 153, 410, 1445, 1691, 3300
BseSI GKGCMC 4 cut(s) 268, 1831, 2078, 2707
BseXI GCAGC 3 cut(s) 1337, 2127, 2407
BsgI GTGCAG 1 cut(s) 2068
BshFI GGCC 5 cut(s) 874, 2000, 2251, 3256, 3332
BshNI GGYRCC 2 cut(s) 1866, 2702
BsiHKAI GWGCWC 3 cut(s) 268, 1111, 2078
BsiHKCI CYCGRG 4 cut(s) 287, 1317, 3251, 3333
BsiSI CCGG 6 cut(s) 227, 407, 515, 710, 1797, 2699
BslFI GGGAC 5 cut(s) 661, 1538, 2204, 2794, 3162
BsmAI GTCTC 9 cut(s) 54, 329, 406, 516, 3534, 3805, 3849, 4111, 4155
BsmBI CGTCTC 2 cut(s) 54, 516
BsmFI GGGAC 5 cut(s) 661, 1538, 2204, 2794, 3162
BsmI GAATGC 2 cut(s) 2422, 2895
BsnI GGCC 5 cut(s) 874, 2000, 2251, 3256, 3332
Bso31I GGTCTC 1 cut(s) 406
BsoBI CYCGRG 4 cut(s) 287, 1317, 3251, 3333
Bsp119I TTCGAA 2 cut(s) 416, 3524
Bsp1286I GDGCHC 7 cut(s) 268, 1111, 1479, 1693, 1831, 2078, 2707
Bsp13I TCCGGA 1 cut(s) 226
Bsp1720I GCTNAGC 1 cut(s) 72
BspANI GGCC 5 cut(s) 874, 2000, 2251, 3256, 3332
BspEI TCCGGA 1 cut(s) 226
BspHI TCATGA 3 cut(s) 814, 1093, 1516
BspMI ACCTGC 1 cut(s) 3610
BspPI GGATC 8 cut(s) 226, 720, 1272, 1633, 2780, 2793, 3176, 3490
BspQI GCTCTTC 1 cut(s) 1152
BspT104I TTCGAA 2 cut(s) 416, 3524
BspT107I GGYRCC 2 cut(s) 1866, 2702
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 6 cut(s) 887, 1200, 1384, 3079, 3099, 3401
BsrI ACTGG 9 cut(s) 1032, 1891, 1906, 2198, 3242, 3412, 3572, 3863, 4169
BssNAI GTATAC 1 cut(s) 1531
BssNI GRCGYC 1 cut(s) 509
BssSI CACGAG 2 cut(s) 3885, 4191
BssT1I CCWWGG 5 cut(s) 1246, 1343, 2750, 3891, 4197
Bst1107I GTATAC 1 cut(s) 1531
Bst2BI CACGAG 2 cut(s) 3885, 4191
BstACI GRCGYC 1 cut(s) 509
BstAPI GCANNNNNTGC 1 cut(s) 263
BstBI TTCGAA 2 cut(s) 416, 3524
BstC8I GCNNGC 8 cut(s) 617, 1164, 2367, 2925, 3186, 3368, 3756, 4032
BstEII GGTNACC 1 cut(s) 2465
BstENI CCTNNNNNAGG 3 cut(s) 2898, 3808, 4114
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 4 cut(s) 117, 247, 3039, 3382
BstMAI GTCTC 9 cut(s) 54, 329, 406, 516, 3534, 3805, 3849, 4111, 4155
BstNSI RCATGY 7 cut(s) 995, 1120, 1466, 1509, 1880, 2927, 3162
BstPI GGTNACC 1 cut(s) 2465
BstSLI GKGCMC 4 cut(s) 268, 1831, 2078, 2707
BstV1I GCAGC 3 cut(s) 1337, 2127, 2407
BstV2I GAAGAC 4 cut(s) 1883, 2370, 3052, 3120
BstX2I RGATCY 5 cut(s) 231, 1625, 1940, 2785, 3482
BstYI RGATCY 5 cut(s) 231, 1625, 1940, 2785, 3482
BstZ17I GTATAC 1 cut(s) 1531
Bsu36I CCTNAGG 2 cut(s) 2180, 3387
BsuRI GGCC 5 cut(s) 874, 2000, 2251, 3256, 3332
BtrI CACGTC 2 cut(s) 501, 1768
BtsI GCAGTG 1 cut(s) 2491
BtsIMutI CAGTG 7 cut(s) 1118, 1201, 1259, 2491, 2767, 3694, 3970
BveI ACCTGC 1 cut(s) 3610
Cac8I GCNNGC 8 cut(s) 617, 1164, 2367, 2925, 3186, 3368, 3756, 4032
CciI TCATGA 3 cut(s) 814, 1093, 1516
CfoI GCGC 4 cut(s) 117, 247, 3039, 3382
Cfr13I GGNCC 9 cut(s) 675, 1088, 1813, 2808, 3133, 3254, 3331, 3619, 3895
CseI GACGC 1 cut(s) 2825
Csp6I GTAC 5 cut(s) 327, 941, 1867, 3324, 3415
CspCI CAANNNNNGTGG 4 cut(s) 187, 222, 2241, 2276
CviQI GTAC 5 cut(s) 327, 941, 1867, 3324, 3415
DraI TTTAAA 3 cut(s) 1792, 2823, 3558
DriI GACNNNNNGTC 2 cut(s) 2012, 3121
EaeI YGGCCR 1 cut(s) 2249
Eam1105I GACNNNNNGTC 2 cut(s) 2012, 3121
EciI GGCGGA 1 cut(s) 184
Eco130I CCWWGG 5 cut(s) 1246, 1343, 2750, 3891, 4197
Eco147I AGGCCT 1 cut(s) 2000
Eco24I GRGCYC 2 cut(s) 1479, 1693
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 7 cut(s) 675, 1088, 1813, 2808, 3133, 3619, 3895
Eco57I CTGAAG 2 cut(s) 1623, 2580
Eco81I CCTNAGG 2 cut(s) 2180, 3387
Eco88I CYCGRG 4 cut(s) 287, 1317, 3251, 3333
Eco91I GGTNACC 1 cut(s) 2465
EcoNI CCTNNNNNAGG 3 cut(s) 2898, 3808, 4114
EcoO109I RGGNCCY 1 cut(s) 675
EcoO65I GGTNACC 1 cut(s) 2465
EcoRI GAATTC 1 cut(s) 1597
EcoT14I CCWWGG 5 cut(s) 1246, 1343, 2750, 3891, 4197
EcoT22I ATGCAT 2 cut(s) 1507, 3212
EcoT38I GRGCYC 2 cut(s) 1479, 1693
ErhI CCWWGG 5 cut(s) 1246, 1343, 2750, 3891, 4197
Esp3I CGTCTC 2 cut(s) 54, 516
FaqI GGGAC 5 cut(s) 661, 1538, 2204, 2794, 3162
FauI CCCGC 1 cut(s) 2622
FbaI TGATCA 3 cut(s) 1912, 2830, 3292
FblI GTMKAC 1 cut(s) 1530
Fnu4HI GCNGC 6 cut(s) 357, 875, 1326, 2116, 2249, 2421
FriOI GRGCYC 2 cut(s) 1479, 1693
Fsp4HI GCNGC 6 cut(s) 357, 875, 1326, 2116, 2249, 2421
FspAI RTGCGCAY 1 cut(s) 3038
FspBI CTAG 9 cut(s) 9, 1344, 2501, 2741, 2751, 2885, 3174, 3797, 4103
FspI TGCGCA 1 cut(s) 3038
GlaI GCGC 4 cut(s) 116, 246, 3038, 3381
GluI GCNGC 6 cut(s) 357, 875, 1326, 2116, 2249, 2421
GsuI CTGGAG 4 cut(s) 1230, 1377, 1463, 1889
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 5 cut(s) 874, 2000, 2251, 3256, 3332
HapII CCGG 6 cut(s) 227, 407, 515, 710, 1797, 2699
HgaI GACGC 1 cut(s) 2825
HhaI GCGC 4 cut(s) 117, 247, 3039, 3382
Hin1I GRCGYC 1 cut(s) 509
Hin6I GCGC 4 cut(s) 115, 245, 3037, 3380
HinP1I GCGC 4 cut(s) 115, 245, 3037, 3380
HincII GTYRAC 7 cut(s) 1222, 2635, 2737, 3685, 3816, 3961, 4122
HindII GTYRAC 7 cut(s) 1222, 2635, 2737, 3685, 3816, 3961, 4122
HindIII AAGCTT 4 cut(s) 821, 1605, 2041, 3355
HpaI GTTAAC 2 cut(s) 3685, 3961
HpaII CCGG 6 cut(s) 227, 407, 515, 710, 1797, 2699
Hpy99I CGWCG 1 cut(s) 2841
HpyCH4IV ACGT 7 cut(s) 500, 509, 1224, 1538, 1767, 1969, 2774
HpySE526I ACGT 7 cut(s) 500, 509, 1224, 1538, 1767, 1969, 2774
Hsp92I GRCGYC 1 cut(s) 509
HspAI GCGC 4 cut(s) 115, 245, 3037, 3380
KflI GGGWCCC 1 cut(s) 675
Kpn2I TCCGGA 1 cut(s) 226
KpnI GGTACC 1 cut(s) 1870
Ksp22I TGATCA 3 cut(s) 1912, 2830, 3292
KspAI GTTAAC 2 cut(s) 3685, 3961
LguI GCTCTTC 1 cut(s) 1152
LmnI GCTCC 8 cut(s) 10, 379, 1021, 1482, 1696, 1819, 2224, 2752
Lsp1109I GCAGC 3 cut(s) 1337, 2127, 2407
MaeI CTAG 9 cut(s) 9, 1344, 2501, 2741, 2751, 2885, 3174, 3797, 4103
MaeII ACGT 7 cut(s) 500, 509, 1224, 1538, 1767, 1969, 2774
MflI RGATCY 5 cut(s) 231, 1625, 1940, 2785, 3482
MhlI GDGCHC 7 cut(s) 268, 1111, 1479, 1693, 1831, 2078, 2707
MlyI GAGTC 5 cut(s) 185, 481, 572, 787, 2044
MmeI TCCRAC 3 cut(s) 124, 1043, 1839
Mph1103I ATGCAT 2 cut(s) 1507, 3212
MroI TCCGGA 1 cut(s) 226
MroXI GAANNNNTTC 2 cut(s) 1580, 3069
MslI CAYNNNNRTG 5 cut(s) 1296, 1521, 2067, 2458, 2541
MspA1I CMGCKG 1 cut(s) 2118
MspI CCGG 6 cut(s) 227, 407, 515, 710, 1797, 2699
Mva1269I GAATGC 2 cut(s) 2422, 2895
NciI CCSGG 2 cut(s) 1798, 2700
NmuCI GTSAC 6 cut(s) 496, 1009, 1648, 2465, 2534, 3464
NsbI TGCGCA 1 cut(s) 3038
NsiI ATGCAT 2 cut(s) 1507, 3212
NspI RCATGY 7 cut(s) 995, 1120, 1466, 1509, 1880, 2927, 3162
NspV TTCGAA 2 cut(s) 416, 3524
PaeI GCATGC 1 cut(s) 2927
PagI TCATGA 3 cut(s) 814, 1093, 1516
PceI AGGCCT 1 cut(s) 2000
PciSI GCTCTTC 1 cut(s) 1152
PcsI WCGNNNNNNNCGW 1 cut(s) 506
PctI GAATGC 2 cut(s) 2422, 2895
PdmI GAANNNNTTC 2 cut(s) 1580, 3069
PfeI GAWTC 9 cut(s) 223, 292, 302, 438, 935, 1097, 1760, 2138, 2489
PflFI GACNNNGTC 2 cut(s) 1254, 3137
PflMI CCANNNNNTGG 1 cut(s) 1700
PkrI GCNGC 6 cut(s) 358, 876, 1327, 2117, 2250, 2422
PleI GAGTC 5 cut(s) 185, 480, 572, 786, 2043
PpsI GAGTC 5 cut(s) 185, 480, 572, 786, 2043
PpuMI RGGWCCY 1 cut(s) 675
PshBI ATTAAT 3 cut(s) 3585, 3870, 4176
Psp1406I AACGTT 2 cut(s) 1224, 1538
Psp5II RGGWCCY 1 cut(s) 675
PspEI GGTNACC 1 cut(s) 2465
PspPI GGNCC 9 cut(s) 675, 1088, 1813, 2808, 3133, 3254, 3331, 3619, 3895
PspPPI RGGWCCY 1 cut(s) 675
PsuI RGATCY 5 cut(s) 231, 1625, 1940, 2785, 3482
PsyI GACNNNGTC 2 cut(s) 1254, 3137
RsaI GTAC 5 cut(s) 328, 942, 1868, 3325, 3416
RsaNI GTAC 5 cut(s) 327, 941, 1867, 3324, 3415
RseI CAYNNNNRTG 5 cut(s) 1296, 1521, 2067, 2458, 2541
SapI GCTCTTC 1 cut(s) 1152
SatI GCNGC 6 cut(s) 357, 875, 1326, 2116, 2249, 2421
Sau96I GGNCC 9 cut(s) 675, 1088, 1813, 2808, 3133, 3254, 3331, 3619, 3895
SchI GAGTC 5 cut(s) 185, 481, 572, 787, 2044
SduI GDGCHC 7 cut(s) 268, 1111, 1479, 1693, 1831, 2078, 2707
SfuI TTCGAA 2 cut(s) 416, 3524
SinI GGWCC 7 cut(s) 675, 1088, 1813, 2808, 3133, 3619, 3895
SmiI ATTTAAAT 1 cut(s) 2823
SmiMI CAYNNNNRTG 5 cut(s) 1296, 1521, 2067, 2458, 2541
SmlI CTYRAG 1 cut(s) 2002
SmoI CTYRAG 1 cut(s) 2002
SpeI ACTAGT 3 cut(s) 3173, 3796, 4102
SphI GCATGC 1 cut(s) 2927
SseBI AGGCCT 1 cut(s) 2000
SspI AATATT 3 cut(s) 1413, 1563, 3276
SspMI CTAG 9 cut(s) 9, 1344, 2501, 2741, 2751, 2885, 3174, 3797, 4103
StuI AGGCCT 1 cut(s) 2000
StyI CCWWGG 5 cut(s) 1246, 1343, 2750, 3891, 4197
SwaI ATTTAAAT 1 cut(s) 2823
TaiI ACGT 7 cut(s) 503, 512, 1227, 1541, 1770, 1972, 2777
TatI WGTACW 1 cut(s) 3323
TauI GCSGC 3 cut(s) 359, 877, 2251
TfiI GAWTC 9 cut(s) 223, 292, 302, 438, 935, 1097, 1760, 2138, 2489
TscAI CASTG 7 cut(s) 1118, 1201, 1259, 2491, 2767, 3694, 3970
TseFI GTSAC 6 cut(s) 496, 1009, 1648, 2465, 2534, 3464
TseI GCWGC 3 cut(s) 1325, 2115, 2420
Tsp45I GTSAC 6 cut(s) 496, 1009, 1648, 2465, 2534, 3464
TspGWI ACGGA 1 cut(s) 2047
TspRI CASTG 7 cut(s) 1118, 1201, 1259, 2491, 2767, 3694, 3970
Tth111I GACNNNGTC 2 cut(s) 1254, 3137
Van91I CCANNNNNTGG 1 cut(s) 1700
VneI GTGCAC 2 cut(s) 264, 2074
VpaK11BI GGWCC 7 cut(s) 675, 1088, 1813, 2808, 3133, 3619, 3895
VspI ATTAAT 3 cut(s) 3585, 3870, 4176
XagI CCTNNNNNAGG 3 cut(s) 2898, 3808, 4114
XapI RAATTY 8 cut(s) 349, 1235, 1597, 2345, 2645, 2818, 2980, 3197
XceI RCATGY 7 cut(s) 995, 1120, 1466, 1509, 1880, 2927, 3162
XmaJI CCTAGG 2 cut(s) 1343, 2750
XmiI GTMKAC 1 cut(s) 1530
XmnI GAANNNNTTC 2 cut(s) 1580, 3069
XspI CTAG 9 cut(s) 9, 1344, 2501, 2741, 2751, 2885, 3174, 3797, 4103
ZraI GACGTC 1 cut(s) 510
Zsp2I ATGCAT 2 cut(s) 1507, 3212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.