Rroxscaffold_2G00136280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004835
Physical Location & Seq
Reverse (-)
74005986 .. 74007661
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136280.1

Sequence Viewer

Length: 408 bp
ATGAAGAATGTGTTGACGGATCCGACGGCGGAGGAGTTTTTGTTCTTCGATTTGATTCCGGCCTTTTTGAAGATTCTCGGGCTGTACAAATTACAAAACGACGGCATCGATGCAAGCGGCTTGTTGGAAGAAGTACTAAGCGAGCAATCACCGGGAAGTTGGGAGAAGCATGGGGTTTTGCTAAAGCGTGACTTTAGCTTCGCCGGGTTGCGAGGGCGTTACCCTTGCTTCGCCGGTAGTATCGGTGGTAGTAGCACCGGTAGTCGGCTCTTGGAGAGACTAGGACCGGAAGTACGGTCGCCGGTTGATCCGGTGATGCGACGGTCGGTTCTTGGAGAGACTAGGACCGGCGCGCGCGGTCACCGGGTTTCAACGAGGTTGAAGGTTTGCTCCGGGGAGGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.74

Weight (kDa)

8.68

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 353, 355, 357
AciI CCGC 3 cut(s) 29, 117, 357
AclWI GGATC 3 cut(s) 14, 27, 302
AfaI GTAC 3 cut(s) 86, 135, 294
AfiI CCNNNNNNNGG 1 cut(s) 264
AgeI ACCGGT 1 cut(s) 257
AgsI TTSAA 3 cut(s) 70, 372, 382
AloI GAACNNNNNNTCC 2 cut(s) 26, 58
AluBI AGCT 1 cut(s) 198
AluI AGCT 1 cut(s) 198
Alw26I GTCTC 2 cut(s) 271, 332
AlwI GGATC 3 cut(s) 14, 27, 302
Ama87I CYCGRG 1 cut(s) 77
AoxI GGCC 1 cut(s) 60
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AsiGI ACCGGT 1 cut(s) 257
AspLEI GCGC 3 cut(s) 353, 355, 357
AspS9I GGNCC 2 cut(s) 284, 345
AsuC2I CCSGG 4 cut(s) 153, 205, 365, 394
AsuHPI GGTGA 3 cut(s) 141, 325, 353
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 2 cut(s) 284, 345
BamHI GGATCC 1 cut(s) 19
BceAI ACGGC 2 cut(s) 42, 118
BcnI CCSGG 4 cut(s) 153, 205, 365, 394
BcoDI GTCTC 2 cut(s) 271, 332
BfaI CTAG 2 cut(s) 281, 342
BisI GCNGC 1 cut(s) 118
BlsI GCNGC 1 cut(s) 119
BmcAI AGTACT 1 cut(s) 135
Bme1390I CCNGG 4 cut(s) 153, 205, 365, 394
Bme18I GGWCC 2 cut(s) 284, 345
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 2 cut(s) 284, 345
BmiI GGNNCC 1 cut(s) 21
BmrFI CCNGG 4 cut(s) 153, 205, 365, 394
BmsI GCATC 3 cut(s) 100, 114, 306
BpuMI CCSGG 4 cut(s) 153, 205, 365, 394
Bsa29I ATCGAT 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 393
BsaWI WCCGGW 3 cut(s) 257, 286, 310
BsaXI ACNNNNNCTCC 3 cut(s) 26, 56, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse118I RCCGGY 4 cut(s) 233, 257, 301, 347
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 393
BseLI CCNNNNNNNGG 1 cut(s) 264
BsePI GCGCGC 2 cut(s) 351, 353
BseRI GAGGAG 1 cut(s) 47
Bsh1236I CGCG 3 cut(s) 353, 355, 357
Bsh1285I CGRYCG 2 cut(s) 299, 326
BshFI GGCC 1 cut(s) 62
BshTI ACCGGT 1 cut(s) 257
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 2 cut(s) 299, 326
BsiHKCI CYCGRG 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 2 cut(s) 271, 332
BsnI GGCC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 77
Bsp1407I TGTACA 1 cut(s) 84
Bsp143I GATC 2 cut(s) 19, 307
BspACI CCGC 3 cut(s) 29, 117, 357
BspANI GGCC 1 cut(s) 62
BspDI ATCGAT 1 cut(s) 108
BspFNI CGCG 3 cut(s) 353, 355, 357
BspLI GGNNCC 1 cut(s) 21
BspPI GGATC 3 cut(s) 14, 27, 302
BsrFI RCCGGY 4 cut(s) 233, 257, 301, 347
BsrGI TGTACA 1 cut(s) 84
BssAI RCCGGY 4 cut(s) 233, 257, 301, 347
BssECI CCNNGG 1 cut(s) 393
BssHII GCGCGC 2 cut(s) 351, 353
BssMI GATC 2 cut(s) 19, 307
Bst4CI ACNGT 2 cut(s) 297, 324
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 4 cut(s) 115, 143, 353, 355
BstDEI CTNAG 1 cut(s) 137
BstEII GGTNACC 1 cut(s) 359
BstFNI CGCG 3 cut(s) 353, 355, 357
BstHHI GCGC 3 cut(s) 353, 355, 357
BstKTI GATC 2 cut(s) 22, 310
BstMAI GTCTC 2 cut(s) 271, 332
BstMBI GATC 2 cut(s) 19, 307
BstMCI CGRYCG 2 cut(s) 299, 326
BstPI GGTNACC 1 cut(s) 359
BstSCI CCNGG 4 cut(s) 151, 203, 363, 392
BstUI CGCG 3 cut(s) 353, 355, 357
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 1 cut(s) 62
BsuTUI ATCGAT 1 cut(s) 108
Cac8I GCNNGC 4 cut(s) 115, 143, 353, 355
CfoI GCGC 3 cut(s) 353, 355, 357
Cfr10I RCCGGY 4 cut(s) 233, 257, 301, 347
Cfr13I GGNCC 2 cut(s) 284, 345
ClaI ATCGAT 1 cut(s) 108
Csp6I GTAC 3 cut(s) 85, 134, 293
CspAI ACCGGT 1 cut(s) 257
CviAII CATG 1 cut(s) 170
CviJI RGCY 6 cut(s) 62, 82, 120, 198, 268, 401
CviKI_1 RGCY 6 cut(s) 62, 82, 120, 198, 268, 401
CviQI GTAC 3 cut(s) 85, 134, 293
DdeI CTNAG 1 cut(s) 137
DpnI GATC 2 cut(s) 21, 309
DpnII GATC 2 cut(s) 19, 307
EciI GGCGGA 1 cut(s) 44
Eco47I GGWCC 2 cut(s) 284, 345
Eco88I CYCGRG 1 cut(s) 77
Eco91I GGTNACC 1 cut(s) 359
EcoO65I GGTNACC 1 cut(s) 359
FaeI CATG 1 cut(s) 173
FaiI YATR 1 cut(s) 171
FalI AAGNNNNNCTT 2 cut(s) 176, 208
FatI CATG 1 cut(s) 169
Fnu4HI GCNGC 1 cut(s) 118
Fsp4HI GCNGC 1 cut(s) 118
FspBI CTAG 2 cut(s) 281, 342
GlaI GCGC 3 cut(s) 352, 354, 356
GluI GCNGC 1 cut(s) 118
HaeIII GGCC 1 cut(s) 62
HhaI GCGC 3 cut(s) 353, 355, 357
Hin1II CATG 1 cut(s) 173
Hin6I GCGC 3 cut(s) 351, 353, 355
HinP1I GCGC 3 cut(s) 351, 353, 355
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 2 cut(s) 55, 73
HphI GGTGA 3 cut(s) 141, 325, 353
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 1 cut(s) 24
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 3 cut(s) 28, 104, 324
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 2 cut(s) 297, 324
HpyCH4V TGCA 1 cut(s) 113
HpyF3I CTNAG 1 cut(s) 137
Hsp92II CATG 1 cut(s) 173
HspAI GCGC 3 cut(s) 351, 353, 355
Kzo9I GATC 2 cut(s) 19, 307
LmnI GCTCC 1 cut(s) 395
LweI GCATC 3 cut(s) 100, 114, 306
MaeI CTAG 2 cut(s) 281, 342
MaeIII GTNAC 3 cut(s) 188, 218, 359
MalI GATC 2 cut(s) 21, 309
MauBI CGCGCGCG 1 cut(s) 353
MboI GATC 2 cut(s) 19, 307
MboII GAAGA 4 cut(s) 16, 37, 82, 140
MflI RGATCY 1 cut(s) 19
MluCI AATT 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 47, 105
MnlI CCTC 4 cut(s) 25, 206, 369, 391
MspR9I CCNGG 4 cut(s) 153, 205, 365, 394
MvnI CGCG 3 cut(s) 353, 355, 357
NciI CCSGG 4 cut(s) 153, 205, 365, 394
NdeII GATC 2 cut(s) 19, 307
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 188, 359
PauI GCGCGC 2 cut(s) 351, 353
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 2 cut(s) 55, 73
PinAI ACCGGT 1 cut(s) 257
PkrI GCNGC 1 cut(s) 119
PspEI GGTNACC 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 2 cut(s) 284, 345
PsuI RGATCY 1 cut(s) 19
PteI GCGCGC 2 cut(s) 351, 353
RsaI GTAC 3 cut(s) 86, 135, 294
RsaNI GTAC 3 cut(s) 85, 134, 293
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 2 cut(s) 19, 307
Sau96I GGNCC 2 cut(s) 284, 345
ScaI AGTACT 1 cut(s) 135
ScrFI CCNGG 4 cut(s) 153, 205, 365, 394
SetI ASST 3 cut(s) 200, 380, 387
SfaNI GCATC 3 cut(s) 100, 114, 306
SinI GGWCC 2 cut(s) 284, 345
Sse9I AATT 1 cut(s) 89
SsiI CCGC 3 cut(s) 29, 117, 357
SspMI CTAG 2 cut(s) 281, 342
StyD4I CCNGG 4 cut(s) 151, 203, 363, 392
TaaI ACNGT 2 cut(s) 297, 324
TaqI TCGA 2 cut(s) 48, 108
TasI AATT 1 cut(s) 89
TatI WGTACW 2 cut(s) 84, 133
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 2 cut(s) 55, 73
TseFI GTSAC 2 cut(s) 188, 359
Tsp45I GTSAC 2 cut(s) 188, 359
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 32
VpaK11BI GGWCC 2 cut(s) 284, 345
XspI CTAG 2 cut(s) 281, 342
ZrmI AGTACT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.