Rorug02G0028800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004835
Physical Location & Seq
Reverse (-)
2280144 .. 2280855
712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0028800.1

Sequence Viewer

Length: 504 bp
ATGAAAACCATGAACATGATAGTTGTTGTTCTCGCAATGGCAGTAGTATTGTTACATGGAGCAGAAGCTGTACTATACACAGTTGGAGACGACTTGGGTTGGGCTATTCCTCCTGGTGGCGCTGCTACTTATGCTGCATGGGCTGCCGAGCATAGCTTTGTAGTCAACGATGAGCTAGAGTTCGACTTTGCAGTAGGTGAACAAGACTTGGCCTTAGTAACCAAGGATGATTACGACAGTTGTAACACGGCGGATCCCTTGGTGCTATTCCAGGAGGCAGTTACACTTCAGTTTATCGCAAGCGACACATTTTATTTCACCTCCACCTTGGCCGGGCACTGCACCAAAGGCCAAAAGATTGCCATTTACATCGGCGCTGCCCTGCCTCCTAGTCCCAGTCCGGTTCCCAGTCCAACTCCATGTCCTTCTTCATCAAGTGCTGATGACGCCCTAACGGTAACGGCCAAATTTGTGTCTCACAAGATCATGGCCAAAGGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

17.59

Weight (kDa)

4.39

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 42 - 118 3e-12 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 251
AclWI GGATC 2 cut(s) 248, 261
AcoI YGGCCR 3 cut(s) 330, 462, 489
AcsI RAATTY 1 cut(s) 467
AcuI CTGAAG 1 cut(s) 272
AcyI GRCGYC 1 cut(s) 447
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 2 cut(s) 116, 333
AjnI CCWGG 2 cut(s) 112, 270
AluBI AGCT 3 cut(s) 68, 156, 175
AluI AGCT 3 cut(s) 68, 156, 175
Alw26I GTCTC 2 cut(s) 81, 480
AlwI GGATC 2 cut(s) 248, 261
AlwNI CAGNNNCTG 1 cut(s) 68
AoxI GGCC 5 cut(s) 210, 330, 349, 462, 489
ApeKI GCWGC 4 cut(s) 122, 134, 143, 377
ApoI RAATTY 1 cut(s) 467
AspLEI GCGC 2 cut(s) 122, 377
AsuC2I CCSGG 1 cut(s) 334
AsuHPI GGTGA 2 cut(s) 209, 310
BaeGI GKGCMC 1 cut(s) 339
BalI TGGCCA 1 cut(s) 491
BamHI GGATCC 1 cut(s) 253
BbvI GCAGC 4 cut(s) 109, 121, 130, 364
BceAI ACGGC 2 cut(s) 264, 477
BciT130I CCWGG 2 cut(s) 114, 272
BcnI CCSGG 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 81, 480
BfaI CTAG 3 cut(s) 176, 390, 502
BfoI RGCGCY 2 cut(s) 123, 378
BisI GCNGC 4 cut(s) 123, 135, 144, 378
BlsI GCNGC 4 cut(s) 124, 136, 145, 379
Bme1390I CCNGG 3 cut(s) 114, 272, 334
BmiI GGNNCC 2 cut(s) 255, 405
BmrFI CCNGG 3 cut(s) 114, 272, 334
BmrI ACTGGG 2 cut(s) 390, 402
BmuI ACTGGG 2 cut(s) 390, 402
BpuMI CCSGG 1 cut(s) 334
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 222, 258, 327
BsaWI WCCGGW 1 cut(s) 400
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 333
Bse1I ACTGG 2 cut(s) 396, 408
Bse3DI GCAATG 1 cut(s) 42
BseBI CCWGG 2 cut(s) 114, 272
BseDI CCNNGG 3 cut(s) 222, 258, 327
BseGI GGATG 1 cut(s) 232
BseLI CCNNNNNNNGG 2 cut(s) 116, 333
BseMI GCAATG 1 cut(s) 42
BseNI ACTGG 2 cut(s) 396, 408
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 4 cut(s) 109, 121, 130, 364
BsgI GTGCAG 1 cut(s) 325
BshFI GGCC 5 cut(s) 212, 332, 351, 464, 491
BsiSI CCGG 2 cut(s) 333, 401
BslFI GGGAC 1 cut(s) 378
BslI CCNNNNNNNGG 2 cut(s) 116, 333
BsmAI GTCTC 2 cut(s) 81, 480
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 5 cut(s) 212, 332, 351, 464, 491
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 2 cut(s) 253, 483
BspACI CCGC 1 cut(s) 251
BspANI GGCC 5 cut(s) 212, 332, 351, 464, 491
BspLI GGNNCC 2 cut(s) 255, 405
BspPI GGATC 2 cut(s) 248, 261
BsrDI GCAATG 1 cut(s) 42
BsrI ACTGG 2 cut(s) 396, 408
BssECI CCNNGG 3 cut(s) 222, 258, 327
BssMI GATC 2 cut(s) 253, 483
BssNI GRCGYC 1 cut(s) 447
BssT1I CCWWGG 3 cut(s) 222, 258, 327
Bst2UI CCWGG 2 cut(s) 114, 272
Bst4CI ACNGT 3 cut(s) 82, 239, 457
BstACI GRCGYC 1 cut(s) 447
BstAPI GCANNNNNTGC 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 1 cut(s) 214
BstF5I GGATG 1 cut(s) 232
BstH2I RGCGCY 2 cut(s) 123, 378
BstHHI GCGC 2 cut(s) 122, 377
BstKTI GATC 2 cut(s) 256, 486
BstMAI GTCTC 2 cut(s) 81, 480
BstMBI GATC 2 cut(s) 253, 483
BstMWI GCNNNNNNNGC 5 cut(s) 131, 140, 143, 348, 446
BstNI CCWGG 2 cut(s) 114, 272
BstSCI CCNGG 3 cut(s) 112, 270, 332
BstSLI GKGCMC 1 cut(s) 339
BstV1I GCAGC 4 cut(s) 109, 121, 130, 364
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 5 cut(s) 212, 332, 351, 464, 491
BtsCI GGATG 1 cut(s) 232
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 337
Cac8I GCNNGC 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 68
CfoI GCGC 2 cut(s) 122, 377
CseI GACGC 1 cut(s) 455
Csp6I GTAC 1 cut(s) 71
CviAII CATG 6 cut(s) 10, 16, 56, 138, 420, 487
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 1 cut(s) 214
DpnI GATC 2 cut(s) 255, 485
DpnII GATC 2 cut(s) 253, 483
EaeI YGGCCR 3 cut(s) 330, 462, 489
EciI GGCGGA 1 cut(s) 266
Eco130I CCWWGG 3 cut(s) 222, 258, 327
Eco57I CTGAAG 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 112, 270
EcoT14I CCWWGG 3 cut(s) 222, 258, 327
ErhI CCWWGG 3 cut(s) 222, 258, 327
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 6 cut(s) 13, 19, 59, 141, 423, 490
FaiI YATR 9 cut(s) 11, 17, 57, 76, 132, 139, 153, 421, 488
FaqI GGGAC 1 cut(s) 378
FatI CATG 6 cut(s) 9, 15, 55, 137, 419, 486
Fnu4HI GCNGC 4 cut(s) 123, 135, 144, 378
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 4 cut(s) 123, 135, 144, 378
FspBI CTAG 3 cut(s) 176, 390, 502
GlaI GCGC 2 cut(s) 121, 376
GluI GCNGC 4 cut(s) 123, 135, 144, 378
HaeII RGCGCY 2 cut(s) 123, 378
HaeIII GGCC 5 cut(s) 212, 332, 351, 464, 491
HapII CCGG 2 cut(s) 333, 401
HgaI GACGC 1 cut(s) 455
HhaI GCGC 2 cut(s) 122, 377
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 6 cut(s) 13, 19, 59, 141, 423, 490
Hin6I GCGC 2 cut(s) 120, 375
HinP1I GCGC 2 cut(s) 120, 375
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HpaII CCGG 2 cut(s) 333, 401
HphI GGTGA 2 cut(s) 209, 310
Hpy166II GTNNAC 2 cut(s) 166, 200
Hpy8I GTNNAC 2 cut(s) 166, 200
HpyAV CCTTC 1 cut(s) 435
HpyCH4III ACNGT 3 cut(s) 82, 239, 457
HpyCH4V TGCA 3 cut(s) 137, 191, 342
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 140, 143, 348, 446
HpyF3I CTNAG 1 cut(s) 214
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 6 cut(s) 13, 19, 59, 141, 423, 490
HspAI GCGC 2 cut(s) 120, 375
Kzo9I GATC 2 cut(s) 253, 483
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 9 cut(s) 99, 126, 257, 284, 346, 395, 409, 414, 421
Lsp1109I GCAGC 4 cut(s) 109, 121, 130, 364
MaeI CTAG 3 cut(s) 176, 390, 502
MaeIII GTNAC 5 cut(s) 51, 217, 242, 280, 457
MalI GATC 2 cut(s) 255, 485
MboI GATC 2 cut(s) 253, 483
MboII GAAGA 1 cut(s) 420
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 339
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 1 cut(s) 467
MluNI TGGCCA 1 cut(s) 491
MmeI TCCRAC 2 cut(s) 64, 437
MnlI CCTC 4 cut(s) 120, 268, 331, 396
Mox20I TGGCCA 1 cut(s) 491
MscI TGGCCA 1 cut(s) 491
MslI CAYNNNNRTG 1 cut(s) 14
Msp20I TGGCCA 1 cut(s) 491
MspI CCGG 2 cut(s) 333, 401
MspR9I CCNGG 3 cut(s) 114, 272, 334
MvaI CCWGG 2 cut(s) 114, 272
MwoI GCNNNNNNNGC 5 cut(s) 131, 140, 143, 348, 446
NciI CCSGG 1 cut(s) 334
NdeII GATC 2 cut(s) 253, 483
NlaIII CATG 6 cut(s) 13, 19, 59, 141, 423, 490
NlaIV GGNNCC 2 cut(s) 255, 405
NmeAIII GCCGAG 1 cut(s) 172
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 4 cut(s) 124, 136, 145, 379
Psp6I CCWGG 2 cut(s) 112, 270
PspGI CCWGG 2 cut(s) 112, 270
PspN4I GGNNCC 2 cut(s) 255, 405
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
RseI CAYNNNNRTG 1 cut(s) 14
SatI GCNGC 4 cut(s) 123, 135, 144, 378
Sau3AI GATC 2 cut(s) 253, 483
ScrFI CCNGG 3 cut(s) 114, 272, 334
SduI GDGCHC 1 cut(s) 339
SetI ASST 6 cut(s) 70, 158, 177, 199, 323, 329
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 1 cut(s) 467
SsiI CCGC 1 cut(s) 251
SspMI CTAG 3 cut(s) 176, 390, 502
StyD4I CCNGG 3 cut(s) 112, 270, 332
StyI CCWWGG 3 cut(s) 222, 258, 327
TaaI ACNGT 3 cut(s) 82, 239, 457
TaqI TCGA 1 cut(s) 183
TasI AATT 1 cut(s) 467
TatI WGTACW 1 cut(s) 70
TscAI CASTG 1 cut(s) 344
TseI GCWGC 4 cut(s) 122, 134, 143, 377
TspDTI ATGAA 3 cut(s) 17, 26, 420
TspRI CASTG 1 cut(s) 344
XapI RAATTY 1 cut(s) 467
XspI CTAG 3 cut(s) 176, 390, 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.