Rroxscaffold_4G00303030

Arginine serine-rich-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000932
Physical Location & Seq
Reverse (-)
23396574 .. 23400394
3821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303030.1

Sequence Viewer

Length: 708 bp
ATGATGATATCGCAGCCAATGGCAGGATTTGCCTTTGTCTATATGGATGATGAGCGAGATGCTGAGTATGCAATTCGAAAGCTTGACAGGACAGAATTTGGTAGGAAGGGACGCCGTCTTCGTATTGAGTGGACCAAGCATGAACGCGGGATTAGGAGATCTGATGATTCAAAAAAACCACCAGCGAACACAAAGCCCTCAAAAACCCTGTTTGTTATCAATTTTGATCCTATCCATACCAGGACAAGGGATCTGGAGAAGCACTTTGATCTATATGGGAAAATAATGAACATAAGGATTAGAAGAAATTTTGCATTTATTCAATATGAATCACAGGAGGATGCTACCAAAGCATTGGATGCAACAAATTCAAGCAAGTTCATGGACCGCGTTATTTCAGTGGAGTATGCTGTTCGTGATGATGATGATAGAAGAAATGGCCACAGTCCTGATAGAAGGGGCCGTGATACGTCTCCTGAGAGGAGAAGAAGCAATGATCGTGGGCGATCTCCAAGTCCATACCGCAGAGATAGGGCTAGTCCGGATTATGGCCATGGTTCCCGTCAGAATTCTAGAGCTGAACCAAGAAGAAGTCCCGAGTATGATAGAGCTCCAAGAAGAAGTCCTGATTATGAGAGAGCCGCAAGCCCAGTCAATGATAGATACCACAGCCGTTCACCCCCAGCACGGGAAAGATCTCGATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05168 map03040 map05168
Pfam Domains
Protein Families
RRM_1 RrnaAD

Protein Analysis

235

Amino Acids

27.8

Weight (kDa)

10.31

Isoelectric Point (pI)

82.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 70 - 132 1.6e-12 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 147, 390
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 4 cut(s) 147, 388, 523, 642
AclWI GGATC 3 cut(s) 221, 258, 696
AcoI YGGCCR 2 cut(s) 439, 550
AcsI RAATTY 4 cut(s) 95, 307, 367, 568
AcyI GRCGYC 1 cut(s) 112
AfiI CCNNNNNNNGG 5 cut(s) 23, 246, 548, 687, 688
AgsI TTSAA 3 cut(s) 171, 323, 372
AjnI CCWGG 1 cut(s) 239
AluBI AGCT 3 cut(s) 82, 578, 611
AluI AGCT 3 cut(s) 82, 578, 611
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 1 cut(s) 477
AlwI GGATC 3 cut(s) 221, 258, 696
Ama87I CYCGRG 1 cut(s) 596
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 3 cut(s) 439, 460, 550
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 4 cut(s) 95, 307, 367, 568
ArsI GACNNNNNNTTYG 2 cut(s) 102, 134
AspS9I GGNCC 3 cut(s) 132, 385, 460
AsuHPI GGTGA 1 cut(s) 669
AsuII TTCGAA 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 596
AvaII GGWCC 2 cut(s) 132, 385
BalI TGGCCA 2 cut(s) 441, 552
BanII GRGCYC 1 cut(s) 613
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 613
BbvI GCAGC 1 cut(s) 25
BceAI ACGGC 3 cut(s) 99, 447, 657
BciT130I CCWGG 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 477
BfaI CTAG 2 cut(s) 537, 573
BglII AGATCT 2 cut(s) 158, 695
BisI GCNGC 2 cut(s) 14, 642
BlsI GCNGC 2 cut(s) 15, 643
Bme1390I CCNGG 1 cut(s) 241
Bme18I GGWCC 2 cut(s) 132, 385
BmeT110I CYCGRG 1 cut(s) 596
BmgT120I GGNCC 3 cut(s) 132, 385, 460
BmiI GGNNCC 2 cut(s) 461, 559
BmrFI CCNGG 1 cut(s) 241
BmrI ACTGGG 1 cut(s) 644
BmsI GCATC 3 cut(s) 49, 331, 349
BmuI ACTGGG 1 cut(s) 644
BpiI GAAGAC 1 cut(s) 110
BpmI CTGGAG 1 cut(s) 275
Bpu14I TTCGAA 1 cut(s) 76
BsaBI GATNNNNATC 1 cut(s) 700
BsaHI GRCGYC 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 553
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 246, 548, 687, 688
Bse1I ACTGG 1 cut(s) 650
Bse3DI GCAATG 1 cut(s) 499
Bse8I GATNNNNATC 1 cut(s) 700
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 1 cut(s) 553
BseGI GGATG 3 cut(s) 52, 346, 364
BseJI GATNNNNATC 1 cut(s) 700
BseLI CCNNNNNNNGG 5 cut(s) 23, 246, 548, 687, 688
BseMI GCAATG 1 cut(s) 499
BseMII CTCAG 2 cut(s) 54, 468
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 1 cut(s) 496
BseXI GCAGC 1 cut(s) 25
BseYI CCCAGC 1 cut(s) 682
Bsh1236I CGCG 2 cut(s) 147, 390
BshFI GGCC 3 cut(s) 441, 462, 552
BsiHKAI GWGCWC 1 cut(s) 613
BsiHKCI CYCGRG 1 cut(s) 596
BsiSI CCGG 1 cut(s) 542
BslFI GGGAC 2 cut(s) 123, 579
BslI CCNNNNNNNGG 5 cut(s) 23, 246, 548, 687, 688
BsmAI GTCTC 1 cut(s) 477
BsmBI CGTCTC 1 cut(s) 477
BsmFI GGGAC 2 cut(s) 123, 579
BsnI GGCC 3 cut(s) 441, 462, 552
BsoBI CYCGRG 1 cut(s) 596
Bsp119I TTCGAA 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 613
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 8 cut(s) 158, 226, 250, 268, 496, 506, 695, 701
Bsp19I CCATGG 1 cut(s) 553
BspACI CCGC 4 cut(s) 147, 388, 523, 642
BspANI GGCC 3 cut(s) 441, 462, 552
BspCNI CTCAG 2 cut(s) 55, 469
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 2 cut(s) 147, 390
BspLI GGNNCC 2 cut(s) 461, 559
BspPI GGATC 3 cut(s) 221, 258, 696
BspT104I TTCGAA 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 499
BsrI ACTGG 1 cut(s) 650
BssECI CCNNGG 1 cut(s) 553
BssMI GATC 8 cut(s) 158, 226, 250, 268, 496, 506, 695, 701
BssNI GRCGYC 1 cut(s) 112
BssT1I CCWWGG 1 cut(s) 553
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 446
BstACI GRCGYC 1 cut(s) 112
BstAPI GCANNNNNTGC 2 cut(s) 29, 359
BstBI TTCGAA 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 2 cut(s) 63, 477
BstDSI CCRYGG 1 cut(s) 553
BstF5I GGATG 3 cut(s) 52, 346, 364
BstFNI CGCG 2 cut(s) 147, 390
BstKTI GATC 8 cut(s) 161, 229, 253, 271, 499, 509, 698, 704
BstMAI GTCTC 1 cut(s) 477
BstMBI GATC 8 cut(s) 158, 226, 250, 268, 496, 506, 695, 701
BstMWI GCNNNNNNNGC 4 cut(s) 29, 68, 350, 359
BstNI CCWGG 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 239
BstUI CGCG 2 cut(s) 147, 390
BstV1I GCAGC 1 cut(s) 25
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 3 cut(s) 158, 250, 695
BstXI CCANNNNNNTGG 1 cut(s) 355
BstYI RGATCY 3 cut(s) 158, 250, 695
BsuRI GGCC 3 cut(s) 441, 462, 552
BtgI CCRYGG 1 cut(s) 553
BtsCI GGATG 3 cut(s) 52, 346, 364
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 1 cut(s) 646
Cfr13I GGNCC 3 cut(s) 132, 385, 460
CseI GACGC 1 cut(s) 120
CspCI CAANNNNNGTGG 2 cut(s) 481, 516
CviAII CATG 3 cut(s) 140, 382, 554
DdeI CTNAG 2 cut(s) 63, 477
DpnI GATC 8 cut(s) 160, 228, 252, 270, 498, 508, 697, 703
DpnII GATC 8 cut(s) 158, 226, 250, 268, 496, 506, 695, 701
EaeI YGGCCR 2 cut(s) 439, 550
Ecl136II GAGCTC 1 cut(s) 611
Eco130I CCWWGG 1 cut(s) 553
Eco24I GRGCYC 1 cut(s) 613
Eco32I GATATC 1 cut(s) 9
Eco47I GGWCC 2 cut(s) 132, 385
Eco53kI GAGCTC 1 cut(s) 611
Eco88I CYCGRG 1 cut(s) 596
EcoICRI GAGCTC 1 cut(s) 611
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 239
EcoRV GATATC 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 553
EcoT38I GRGCYC 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 553
Esp3I CGTCTC 1 cut(s) 477
FaeI CATG 3 cut(s) 143, 385, 557
FaqI GGGAC 2 cut(s) 123, 579
FatI CATG 3 cut(s) 139, 381, 553
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 2 cut(s) 14, 642
FokI GGATG 3 cut(s) 59, 353, 371
FriOI GRGCYC 1 cut(s) 613
Fsp4HI GCNGC 2 cut(s) 14, 642
FspBI CTAG 2 cut(s) 537, 573
GluI GCNGC 2 cut(s) 14, 642
GsaI CCCAGC 1 cut(s) 686
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 3 cut(s) 441, 462, 552
HapII CCGG 1 cut(s) 542
HgaI GACGC 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 112
Hin1II CATG 3 cut(s) 143, 385, 557
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 2 cut(s) 167, 329
HpaII CCGG 1 cut(s) 542
HphI GGTGA 1 cut(s) 669
Hpy166II GTNNAC 2 cut(s) 132, 677
Hpy188I TCNGA 2 cut(s) 163, 567
Hpy8I GTNNAC 2 cut(s) 132, 677
HpyAV CCTTC 2 cut(s) 100, 450
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 3 cut(s) 71, 314, 362
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 68, 350, 359
HpyF3I CTNAG 2 cut(s) 63, 477
HpySE526I ACGT 1 cut(s) 470
Hsp92I GRCGYC 1 cut(s) 112
Hsp92II CATG 3 cut(s) 143, 385, 557
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 8 cut(s) 158, 226, 250, 268, 496, 506, 695, 701
LmnI GCTCC 1 cut(s) 616
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 3 cut(s) 49, 331, 349
MaeI CTAG 2 cut(s) 537, 573
MaeII ACGT 1 cut(s) 470
MalI GATC 8 cut(s) 160, 228, 252, 270, 498, 508, 697, 703
MboI GATC 8 cut(s) 158, 226, 250, 268, 496, 506, 695, 701
MboII GAAGA 6 cut(s) 110, 315, 444, 498, 600, 630
MflI RGATCY 3 cut(s) 158, 250, 695
MhlI GDGCHC 1 cut(s) 613
MlsI TGGCCA 2 cut(s) 441, 552
MluCI AATT 6 cut(s) 72, 95, 220, 307, 367, 568
MluNI TGGCCA 2 cut(s) 441, 552
MnlI CCTC 3 cut(s) 208, 331, 474
Mox20I TGGCCA 2 cut(s) 441, 552
MroI TCCGGA 1 cut(s) 541
MscI TGGCCA 2 cut(s) 441, 552
Msp20I TGGCCA 2 cut(s) 441, 552
MspI CCGG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 241
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 2 cut(s) 147, 390
MwoI GCNNNNNNNGC 4 cut(s) 29, 68, 350, 359
NcoI CCATGG 1 cut(s) 553
NdeII GATC 8 cut(s) 158, 226, 250, 268, 496, 506, 695, 701
NlaIII CATG 3 cut(s) 143, 385, 557
NlaIV GGNNCC 2 cut(s) 461, 559
NspV TTCGAA 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 118
PfeI GAWTC 2 cut(s) 167, 329
PflFI GACNNNGTC 1 cut(s) 114
PkrI GCNGC 2 cut(s) 15, 643
Psp124BI GAGCTC 1 cut(s) 613
Psp6I CCWGG 1 cut(s) 239
PspFI CCCAGC 1 cut(s) 682
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 2 cut(s) 461, 559
PspPI GGNCC 3 cut(s) 132, 385, 460
PsuI RGATCY 3 cut(s) 158, 250, 695
PsyI GACNNNGTC 1 cut(s) 114
SacI GAGCTC 1 cut(s) 613
SatI GCNGC 2 cut(s) 14, 642
Sau3AI GATC 8 cut(s) 158, 226, 250, 268, 496, 506, 695, 701
Sau96I GGNCC 3 cut(s) 132, 385, 460
ScrFI CCNGG 1 cut(s) 241
SduI GDGCHC 1 cut(s) 613
SetI ASST 4 cut(s) 84, 473, 580, 613
SfaNI GCATC 3 cut(s) 49, 331, 349
SfuI TTCGAA 1 cut(s) 76
SinI GGWCC 2 cut(s) 132, 385
Sse9I AATT 6 cut(s) 72, 95, 220, 307, 367, 568
SsiI CCGC 4 cut(s) 147, 388, 523, 642
SspMI CTAG 2 cut(s) 537, 573
SstI GAGCTC 1 cut(s) 613
StyD4I CCNGG 1 cut(s) 239
StyI CCWWGG 1 cut(s) 553
TaaI ACNGT 1 cut(s) 446
TaiI ACGT 1 cut(s) 473
TaqI TCGA 2 cut(s) 76, 700
TasI AATT 6 cut(s) 72, 95, 220, 307, 367, 568
TauI GCSGC 1 cut(s) 644
TfiI GAWTC 2 cut(s) 167, 329
TscAI CASTG 1 cut(s) 405
TseI GCWGC 1 cut(s) 13
TspDTI ATGAA 4 cut(s) 156, 302, 342, 370
TspRI CASTG 1 cut(s) 405
Tth111I GACNNNGTC 1 cut(s) 114
VpaK11BI GGWCC 2 cut(s) 132, 385
XapI RAATTY 4 cut(s) 95, 307, 367, 568
XbaI TCTAGA 1 cut(s) 572
XspI CTAG 2 cut(s) 537, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.