Rw1G020950

Arginine serine-rich-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000932
Physical Location & Seq
Forward (+)
44772414 .. 44776338
3925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020950.1

Sequence Viewer

Length: 666 bp
ATGGATGATGAGCGAGATGCTGAGTATGCAATTCGAAAGCTTGACAGGACAGAATTTGGTAGGAAGGGACGCCGTCTTCGTATTGAGTGGACCAAGCATGAACGCGGGATTAGGAGATCTGATGATTCAAAAAAACCACCAGCGAACACAAAGCCCTCAAAAACCCTGTTTGTTATCAATTTTGATCCTATCCATACCAGGACAAGGGATCTGGAGAAGCACTTTGATCTATATGGGAAAATAATGAACATAAGGATTAGAAGAAATTTTGCATTTATTCAATATGAATCACAGGAGGATGCTACCAAAGCATTGGATGCAACAAATTCAAGCAAGTTCATGGACCGCGTTATTTCAGTGGAGTATGCTGTTCGTGATGATGATGATAGAAGAAATGGCCACAGTCCTGATAGAAGGGGCCGTGATACGTCTCCTGAGAGGAGAAGAAGCAATGATCGTGGGCGATCTCCAAGTCCATACCGCAGAGATAGGGCTAGTCCGGATTATGGCCATGGTTCCCGTCAGAATTCTAGAGCTGAACCAAGAAGAAGTCCTGAGTATGATAGAGCTCCAAGAAGAAGTCCTGATTATGAGAGAGCCGCAAGCCCAGTCAATGATAGATACCACAGCCGTTCACCCCCAGCGCGGGAAAGATCTCGATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05168 map03040 map05168
Pfam Domains
Protein Families
RRM_1 RrnaAD

Protein Analysis

221

Amino Acids

26.22

Weight (kDa)

10.37

Isoelectric Point (pI)

83.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 56 - 118 1.4e-12 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 105, 348, 646
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 5 cut(s) 105, 346, 481, 600, 646
AclWI GGATC 3 cut(s) 179, 216, 654
AcoI YGGCCR 2 cut(s) 397, 508
AcsI RAATTY 4 cut(s) 53, 265, 325, 526
AcyI GRCGYC 1 cut(s) 70
AfiI CCNNNNNNNGG 4 cut(s) 204, 506, 645, 646
AgsI TTSAA 3 cut(s) 129, 281, 330
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 3 cut(s) 40, 536, 569
AluI AGCT 3 cut(s) 40, 536, 569
Alw21I GWGCWC 1 cut(s) 571
Alw26I GTCTC 1 cut(s) 435
AlwI GGATC 3 cut(s) 179, 216, 654
Aor13HI TCCGGA 1 cut(s) 499
AoxI GGCC 3 cut(s) 397, 418, 508
ApoI RAATTY 4 cut(s) 53, 265, 325, 526
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
AspLEI GCGC 1 cut(s) 646
AspS9I GGNCC 3 cut(s) 90, 343, 418
AsuHPI GGTGA 1 cut(s) 627
AsuII TTCGAA 1 cut(s) 34
AvaII GGWCC 2 cut(s) 90, 343
BalI TGGCCA 2 cut(s) 399, 510
BanII GRGCYC 1 cut(s) 571
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 571
BceAI ACGGC 3 cut(s) 57, 405, 615
BciT130I CCWGG 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 2 cut(s) 495, 531
BglII AGATCT 2 cut(s) 116, 653
BisI GCNGC 1 cut(s) 600
BlsI GCNGC 1 cut(s) 601
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 2 cut(s) 90, 343
BmgT120I GGNCC 3 cut(s) 90, 343, 418
BmiI GGNNCC 2 cut(s) 419, 517
BmrFI CCNGG 1 cut(s) 199
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 3 cut(s) 7, 289, 307
BmuI ACTGGG 1 cut(s) 602
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 233
Bpu14I TTCGAA 1 cut(s) 34
BsaBI GATNNNNATC 1 cut(s) 658
BsaHI GRCGYC 1 cut(s) 70
BsaJI CCNNGG 1 cut(s) 511
BsaWI WCCGGW 1 cut(s) 499
Bsc4I CCNNNNNNNGG 4 cut(s) 204, 506, 645, 646
Bse1I ACTGG 1 cut(s) 608
Bse3DI GCAATG 1 cut(s) 457
Bse8I GATNNNNATC 1 cut(s) 658
BseAI TCCGGA 1 cut(s) 499
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 3 cut(s) 10, 304, 322
BseJI GATNNNNATC 1 cut(s) 658
BseLI CCNNNNNNNGG 4 cut(s) 204, 506, 645, 646
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 3 cut(s) 12, 426, 546
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 1 cut(s) 454
BseYI CCCAGC 1 cut(s) 640
Bsh1236I CGCG 3 cut(s) 105, 348, 646
BshFI GGCC 3 cut(s) 399, 420, 510
BsiHKAI GWGCWC 1 cut(s) 571
BsiSI CCGG 1 cut(s) 500
BslFI GGGAC 1 cut(s) 81
BslI CCNNNNNNNGG 4 cut(s) 204, 506, 645, 646
BsmAI GTCTC 1 cut(s) 435
BsmBI CGTCTC 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 81
BsnI GGCC 3 cut(s) 399, 420, 510
Bsp119I TTCGAA 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 571
Bsp13I TCCGGA 1 cut(s) 499
Bsp143I GATC 8 cut(s) 116, 184, 208, 226, 454, 464, 653, 659
Bsp19I CCATGG 1 cut(s) 511
BspACI CCGC 5 cut(s) 105, 346, 481, 600, 646
BspANI GGCC 3 cut(s) 399, 420, 510
BspCNI CTCAG 3 cut(s) 13, 427, 547
BspEI TCCGGA 1 cut(s) 499
BspFNI CGCG 3 cut(s) 105, 348, 646
BspLI GGNNCC 2 cut(s) 419, 517
BspPI GGATC 3 cut(s) 179, 216, 654
BspT104I TTCGAA 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 457
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 511
BssMI GATC 8 cut(s) 116, 184, 208, 226, 454, 464, 653, 659
BssNI GRCGYC 1 cut(s) 70
BssT1I CCWWGG 1 cut(s) 511
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 1 cut(s) 404
BstACI GRCGYC 1 cut(s) 70
BstAPI GCANNNNNTGC 1 cut(s) 317
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 604
BstDEI CTNAG 3 cut(s) 21, 435, 555
BstDSI CCRYGG 1 cut(s) 511
BstF5I GGATG 3 cut(s) 10, 304, 322
BstFNI CGCG 3 cut(s) 105, 348, 646
BstHHI GCGC 1 cut(s) 646
BstKTI GATC 8 cut(s) 119, 187, 211, 229, 457, 467, 656, 662
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 8 cut(s) 116, 184, 208, 226, 454, 464, 653, 659
BstMWI GCNNNNNNNGC 3 cut(s) 26, 308, 317
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 197
BstUI CGCG 3 cut(s) 105, 348, 646
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 3 cut(s) 116, 208, 653
BstXI CCANNNNNNTGG 1 cut(s) 313
BstYI RGATCY 3 cut(s) 116, 208, 653
BsuRI GGCC 3 cut(s) 399, 420, 510
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 3 cut(s) 10, 304, 322
BtsIMutI CAGTG 1 cut(s) 363
Cac8I GCNNGC 1 cut(s) 604
CfoI GCGC 1 cut(s) 646
Cfr13I GGNCC 3 cut(s) 90, 343, 418
CseI GACGC 1 cut(s) 78
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviAII CATG 3 cut(s) 98, 340, 512
DdeI CTNAG 3 cut(s) 21, 435, 555
DpnI GATC 8 cut(s) 118, 186, 210, 228, 456, 466, 655, 661
DpnII GATC 8 cut(s) 116, 184, 208, 226, 454, 464, 653, 659
EaeI YGGCCR 2 cut(s) 397, 508
Ecl136II GAGCTC 1 cut(s) 569
Eco130I CCWWGG 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 571
Eco47I GGWCC 2 cut(s) 90, 343
Eco53kI GAGCTC 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 569
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 511
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 3 cut(s) 101, 343, 515
FaqI GGGAC 1 cut(s) 81
FatI CATG 3 cut(s) 97, 339, 511
FauI CCCGC 2 cut(s) 98, 639
Fnu4HI GCNGC 1 cut(s) 600
FokI GGATG 3 cut(s) 17, 311, 329
FriOI GRGCYC 1 cut(s) 571
Fsp4HI GCNGC 1 cut(s) 600
FspBI CTAG 2 cut(s) 495, 531
GlaI GCGC 1 cut(s) 645
GluI GCNGC 1 cut(s) 600
GsaI CCCAGC 1 cut(s) 644
GsuI CTGGAG 1 cut(s) 233
HaeIII GGCC 3 cut(s) 399, 420, 510
HapII CCGG 1 cut(s) 500
HgaI GACGC 1 cut(s) 78
HhaI GCGC 1 cut(s) 646
Hin1I GRCGYC 1 cut(s) 70
Hin1II CATG 3 cut(s) 101, 343, 515
Hin6I GCGC 1 cut(s) 644
HinP1I GCGC 1 cut(s) 644
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 2 cut(s) 125, 287
HpaII CCGG 1 cut(s) 500
HphI GGTGA 1 cut(s) 627
Hpy166II GTNNAC 2 cut(s) 90, 635
Hpy188I TCNGA 2 cut(s) 121, 525
Hpy8I GTNNAC 2 cut(s) 90, 635
HpyAV CCTTC 2 cut(s) 58, 408
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 3 cut(s) 29, 272, 320
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 308, 317
HpyF3I CTNAG 3 cut(s) 21, 435, 555
HpySE526I ACGT 1 cut(s) 428
Hsp92I GRCGYC 1 cut(s) 70
Hsp92II CATG 3 cut(s) 101, 343, 515
HspAI GCGC 1 cut(s) 644
Kpn2I TCCGGA 1 cut(s) 499
Kzo9I GATC 8 cut(s) 116, 184, 208, 226, 454, 464, 653, 659
LmnI GCTCC 1 cut(s) 574
LweI GCATC 3 cut(s) 7, 289, 307
MaeI CTAG 2 cut(s) 495, 531
MaeII ACGT 1 cut(s) 428
MalI GATC 8 cut(s) 118, 186, 210, 228, 456, 466, 655, 661
MboI GATC 8 cut(s) 116, 184, 208, 226, 454, 464, 653, 659
MboII GAAGA 6 cut(s) 68, 273, 402, 456, 558, 588
MflI RGATCY 3 cut(s) 116, 208, 653
MhlI GDGCHC 1 cut(s) 571
MlsI TGGCCA 2 cut(s) 399, 510
MluCI AATT 6 cut(s) 30, 53, 178, 265, 325, 526
MluNI TGGCCA 2 cut(s) 399, 510
MnlI CCTC 3 cut(s) 166, 289, 432
Mox20I TGGCCA 2 cut(s) 399, 510
MroI TCCGGA 1 cut(s) 499
MscI TGGCCA 2 cut(s) 399, 510
Msp20I TGGCCA 2 cut(s) 399, 510
MspI CCGG 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 199
MvaI CCWGG 1 cut(s) 199
MvnI CGCG 3 cut(s) 105, 348, 646
MwoI GCNNNNNNNGC 3 cut(s) 26, 308, 317
NcoI CCATGG 1 cut(s) 511
NdeII GATC 8 cut(s) 116, 184, 208, 226, 454, 464, 653, 659
NlaIII CATG 3 cut(s) 101, 343, 515
NlaIV GGNNCC 2 cut(s) 419, 517
NspV TTCGAA 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PfeI GAWTC 2 cut(s) 125, 287
PflFI GACNNNGTC 1 cut(s) 72
PkrI GCNGC 1 cut(s) 601
Psp124BI GAGCTC 1 cut(s) 571
Psp6I CCWGG 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 640
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 419, 517
PspPI GGNCC 3 cut(s) 90, 343, 418
PsuI RGATCY 3 cut(s) 116, 208, 653
PsyI GACNNNGTC 1 cut(s) 72
SacI GAGCTC 1 cut(s) 571
SatI GCNGC 1 cut(s) 600
Sau3AI GATC 8 cut(s) 116, 184, 208, 226, 454, 464, 653, 659
Sau96I GGNCC 3 cut(s) 90, 343, 418
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 1 cut(s) 571
SetI ASST 4 cut(s) 42, 431, 538, 571
SfaNI GCATC 3 cut(s) 7, 289, 307
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 2 cut(s) 90, 343
Sse9I AATT 6 cut(s) 30, 53, 178, 265, 325, 526
SsiI CCGC 5 cut(s) 105, 346, 481, 600, 646
SspMI CTAG 2 cut(s) 495, 531
SstI GAGCTC 1 cut(s) 571
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 1 cut(s) 404
TaiI ACGT 1 cut(s) 431
TaqI TCGA 2 cut(s) 34, 658
TasI AATT 6 cut(s) 30, 53, 178, 265, 325, 526
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 2 cut(s) 125, 287
TscAI CASTG 1 cut(s) 363
TspDTI ATGAA 4 cut(s) 114, 260, 300, 328
TspRI CASTG 1 cut(s) 363
Tth111I GACNNNGTC 1 cut(s) 72
VpaK11BI GGWCC 2 cut(s) 90, 343
XapI RAATTY 4 cut(s) 53, 265, 325, 526
XbaI TCTAGA 1 cut(s) 530
XspI CTAG 2 cut(s) 495, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.