Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0002536

Physical Location & Seq

Genomic Coordinates Forward (+)

66708411 .. 66710806

Gene Structure 2396 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0538300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGGAAACAAGATCTGCCGCTGCCAAGAAGAAAAAGCTTTTCTTCTGCAAAGAAAATGAACACAGCCACAACAATATTGACCGCATCACCGAACTCCCCGACGCCGTTCTCCACCACATTCTCTTCCTCCTCCCCATCAAAACCGTCGCGCAAACCAGCGTCTTGTCAAAACGGTGGCGTTCGCTCTGGTCGTCCTTCCCGGACCTCGACTTCACAACCCTAAACCCATTTCCGAAACCATGTTCCTCATCCAAGTCACATTCTCTGAATTGCAAAGAGTCCGACTTTATCACTCAGGTCCTCAACAACCGAGACAAGCACTCCGATATTCGGATTCTCCGGTTCCGGGCCCGGCTGAGCTTTTCACGTCTCAACGGTCTGATCCGGCTCGCAATCAGACACAAGGTGCAGGTGCTCGACGTGGAGGTTTTCACAGAGGATTACTTCAACTTCCCTAGATGTGTCATCGCCAGCGACTCATTAAGGGTTTTCAAGTTAAAGTCCCGGAGACCCGGTTTCCGGTTACCACCTTCTTCTGTAATGACAAGCGGTTTCCGATCTCTTCAGTCTTTATCGCTTTCCCGGATCAGTCTATGCAACCAGCCCTCTCTCTCCGATATGTTCTCCGAGTCGTCCTTCCTTCTGCTCAAGAAACTGAGCCTCGACACGTGTTTTGGGTTGAAGCATCTTAGGGTTGGGTGCCGTGCGCTGCAAGACTTAACCCTAGAGAACTGTTTCGAGCTGCAAGGGCTGGATGTTTCGGGTTCGAAATTGGAGAGGTTGCGGATTGCGAAATGTTTTGATGGGTACATTGAGAAGAGTTGGGTGAAGATTGATGCGCCGAGGCTTGGAGTGGTTTTGTGGGAACACAATGCTCTTAGTGATAATATCTGTATGGAGAATCTGAGTTACTTGCATAAGGCCTCCATTGGTATGCTCCATGAAGATGTTAGTGTTGCAAAGCTTCAAAGTGTGTCCAATCTTTTTTCGGCTCTCTCTCTTGCACATTGCTTGACACTCGAAAGCAATTGTATTGAGATTCTATCAAACAACAATTATTTTGCAGTTTATTTGCATCAGTTTAGCAATCTGAAATATTTGGAGTTGCACACGGGTTTCAAGAAGAACAATGTCCCTGGATTAGCATCCATATTCAGGAGTTGTCCTATGCTTCACACTTTGATTCTCAACATTATTAACGATTTCAAAACTGAAAGGAGACAATGGAACAAGGATTTGTGGGAAATGTCTAACTCAGAGGAAGAGAAATACTGGGAATCCCAAACCCTAAACTTGAAGTTGTTCTTGCATCACCTTAAGATAGTGAAGATTCTTGGGTTTTTAGAGTGCGAGAATGAAGTGAGCCTGGCAAAGTTCTTGCTCAAACATGGAAAGGCCTTGGAAGAAATGATTTTGTCCACCGGGCACTGCAAAGCTAGACACTCTCTTCAGAGACAAAAGATAAGGTCACAGATGATGGGATTTTCTTGGGCTTCTTCTAATGCTAAAATTACATTTCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009636 GO:0009987 GO:0015035 GO:0015036 GO:0016020 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0016671 GO:0019725 GO:0033554 GO:0034599 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045454 GO:0047134 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071944 GO:0097237 GO:0098754 GO:0098869 GO:1990748

KEGG Pathways

Metabolic & Signaling

ko04621 ko05418 map04621 map05418

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

507

Amino Acids

58.34

Weight (kDa)

9.32

Isoelectric Point (pI)

47.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	29 - 67	3.2e-08	F-box domain
LRR_At1g61320_AtMIF1	PF23622	118 - 264	5.4e-10	At1g61320/AtMIF1, LRR domain
LRR_At1g61320_AtMIF1	PF23622	234 - 488	1.1e-12	At1g61320/AtMIF1, LRR domain
FBD	PF08387	435 - 472	3.7e-07	FBD

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002536)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G39950
fragaria_vesca	FvH4_4g35660 FvH4_6g49350 FvH4_6g49370
malus_domestica	MD09G1045800.v1.1 MD13G1080800.v1.1 MD13G1081000.v1.1 MD13G1081100.v1.1 MD13G1081200.v1.1 MD16G1080300.v1.1 MD16G1080400.v1.1 MD17G1046700.v1.1
prunus_persica	Prupe.1G265600_v2.0.a1 Prupe.3G272400_v2.0.a1 Prupe.3G272400_v2.0.a1
pyrus_communis	pycom111g03550 pycom13g07000 pycom16g06950 pycom16g06960 pycom17g04160
rosa_chinensis	RchiOBHm_Chr2g0169221 RchiOBHm_Chr4g0445271
rosa_laevigata	RLG00000005753 RLG00000021843
rosa_multiflora	Rmu_sc0000050.1_g000025 Rmu_sc0036229.1_g000001 Rmu_ssc0000440.1_g000008
rosa_roxburghii	Rroxscaffold_2G00082340 Rroxscaffold_5G00385780
rosa_rugosa	Rorug02G0538200 Rorug02G0538300 Rorug04G0360000
rosa_samantha	Rh2AG609800 Rh2BG622700 Rh2CG591200 Rh2DG633500 Rh4AG421400 Rh4BG433300 Rh4CG448000 Rh4DG429200
rosa_wichuraiana	Rw4G036140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	400
Acc36I	ACCTGC	1 cut(s)	400
AccB1I	GGYRCC	1 cut(s)	699
AccII	CGCG	1 cut(s)	149
AciI	CCGC	4 cut(s)	18, 82, 549, 784
AclWI	GGATC	2 cut(s)	376, 593
AcuI	CTGAAG	2 cut(s)	548, 1433
AcvI	CACGTG	1 cut(s)	669
AcyI	GRCGYC	1 cut(s)	102
AdeI	CACNNNGTG	1 cut(s)	406
AfaI	GTAC	1 cut(s)	809
AfiI	CCNNNNNNNGG	5 cut(s)	330, 346, 519, 848, 1155
AflII	CTTAAG	1 cut(s)	1316
AflIII	ACRYGT	2 cut(s)	666, 668
AgsI	TTSAA	7 cut(s)	448, 493, 682, 968, 1120, 1207, 1297
AjiI	CACGTC	2 cut(s)	368, 421
AjnI	CCWGG	2 cut(s)	1135, 1365
AluBI	AGCT	5 cut(s)	37, 360, 742, 964, 1436
AluI	AGCT	5 cut(s)	37, 360, 742, 964, 1436
Alw21I	GWGCWC	1 cut(s)	417
Alw26I	GTCTC	5 cut(s)	306, 374, 502, 1213, 1447
AlwI	GGATC	2 cut(s)	376, 593
AoxI	GGCC	3 cut(s)	348, 921, 1395
ApaI	GGGCCC	1 cut(s)	352
ApeKI	GCWGC	3 cut(s)	20, 709, 742
ArsI	GACNNNNNNTTYG	2 cut(s)	656, 688
Asp700I	GAANNNNTTC	2 cut(s)	734, 1301
AspLEI	GCGC	3 cut(s)	151, 709, 841
AspS9I	GGNCC	4 cut(s)	202, 298, 348, 349
AsuC2I	CCSGG	7 cut(s)	200, 347, 352, 505, 513, 583, 1423
AsuHPI	GGTGA	3 cut(s)	79, 838, 1304
AsuII	TTCGAA	1 cut(s)	767
AvaII	GGWCC	2 cut(s)	202, 298
BaeGI	GKGCMC	2 cut(s)	352, 1428
BanI	GGYRCC	1 cut(s)	699
BanII	GRGCYC	1 cut(s)	352
BbrPI	CACGTG	1 cut(s)	669
Bbv12I	GWGCWC	1 cut(s)	417
BbvI	GCAGC	3 cut(s)	7, 696, 729
BccI	CCATC	3 cut(s)	143, 797, 1471
BceAI	ACGGC	2 cut(s)	89, 687
BciT130I	CCWGG	2 cut(s)	1137, 1367
BcnI	CCSGG	7 cut(s)	200, 347, 352, 505, 513, 583, 1423
BcoDI	GTCTC	5 cut(s)	306, 374, 502, 1213, 1447
BfaI	CTAG	3 cut(s)	456, 725, 1437
BfrI	CTTAAG	1 cut(s)	1316
BfuAI	ACCTGC	1 cut(s)	400
BglII	AGATCT	1 cut(s)	11
BisI	GCNGC	4 cut(s)	18, 21, 710, 743
BlpI	GCTNAGC	1 cut(s)	356
BlsI	GCNGC	4 cut(s)	19, 22, 711, 744
Bme1390I	CCNGG	9 cut(s)	200, 347, 352, 505, 513, 583, 1137, 1367, 1423
Bme18I	GGWCC	2 cut(s)	202, 298
BmgBI	CACGTC	2 cut(s)	368, 421
BmgT120I	GGNCC	4 cut(s)	202, 298, 348, 349
BmiI	GGNNCC	3 cut(s)	344, 350, 701
BmrFI	CCNGG	9 cut(s)	200, 347, 352, 505, 513, 583, 1137, 1367, 1423
BmrI	ACTGGG	1 cut(s)	1282
BmsI	GCATC	6 cut(s)	93, 694, 826, 1084, 1154, 1318
BmuI	ACTGGG	1 cut(s)	1282
Bpu1102I	GCTNAGC	1 cut(s)	356
Bpu14I	TTCGAA	1 cut(s)	767
BpuEI	CTTGAG	1 cut(s)	632
BpuMI	CCSGG	7 cut(s)	200, 347, 352, 505, 513, 583, 1423
BsaAI	YACGTR	1 cut(s)	669
BsaBI	GATNNNNATC	1 cut(s)	1144
BsaHI	GRCGYC	1 cut(s)	102
BsaI	GGTCTC	1 cut(s)	502
BsaJI	CCNNGG	3 cut(s)	842, 1135, 1398
BsaWI	WCCGGW	2 cut(s)	339, 519
BsaXI	ACNNNNNCTCC	4 cut(s)	305, 335, 499, 529
Bsc4I	CCNNNNNNNGG	5 cut(s)	330, 346, 519, 848, 1155
Bse1I	ACTGG	1 cut(s)	1277
Bse3DI	GCAATG	1 cut(s)	1006
Bse8I	GATNNNNATC	1 cut(s)	1144
BseBI	CCWGG	2 cut(s)	1137, 1367
BseDI	CCNNGG	3 cut(s)	842, 1135, 1398
BseGI	GGATG	3 cut(s)	248, 760, 1145
BseJI	GATNNNNATC	1 cut(s)	1144
BseLI	CCNNNNNNNGG	5 cut(s)	330, 346, 519, 848, 1155
BseMI	GCAATG	1 cut(s)	1006
BseMII	CTCAG	5 cut(s)	308, 347, 647, 896, 1269
BseNI	ACTGG	1 cut(s)	1277
BseRI	GAGGAG	1 cut(s)	119
BseSI	GKGCMC	2 cut(s)	352, 1428
BseXI	GCAGC	3 cut(s)	7, 696, 729
BsgI	GTGCAG	1 cut(s)	428
Bsh1236I	CGCG	1 cut(s)	149
BshFI	GGCC	3 cut(s)	350, 923, 1397
BshNI	GGYRCC	1 cut(s)	699
BsiHKAI	GWGCWC	1 cut(s)	417
BslFI	GGGAC	2 cut(s)	487, 1118
BslI	CCNNNNNNNGG	5 cut(s)	330, 346, 519, 848, 1155
BsmAI	GTCTC	5 cut(s)	306, 374, 502, 1213, 1447
BsmBI	CGTCTC	1 cut(s)	374
BsmFI	GGGAC	2 cut(s)	487, 1118
BsnI	GGCC	3 cut(s)	350, 923, 1397
Bso31I	GGTCTC	1 cut(s)	502
Bsp119I	TTCGAA	1 cut(s)	767
Bsp120I	GGGCCC	1 cut(s)	348
Bsp1286I	GDGCHC	3 cut(s)	352, 417, 1428
Bsp143I	GATC	4 cut(s)	11, 381, 557, 585
Bsp1720I	GCTNAGC	1 cut(s)	356
BspACI	CCGC	4 cut(s)	18, 82, 549, 784
BspANI	GGCC	3 cut(s)	350, 923, 1397
BspCNI	CTCAG	5 cut(s)	307, 348, 648, 897, 1268
BspFNI	CGCG	1 cut(s)	149
BspLI	GGNNCC	3 cut(s)	344, 350, 701
BspMI	ACCTGC	1 cut(s)	400
BspPI	GGATC	2 cut(s)	376, 593
BspT104I	TTCGAA	1 cut(s)	767
BspT107I	GGYRCC	1 cut(s)	699
BspTI	CTTAAG	1 cut(s)	1316
BspTNI	GGTCTC	1 cut(s)	502
BsrDI	GCAATG	1 cut(s)	1006
BsrI	ACTGG	1 cut(s)	1277
BssECI	CCNNGG	3 cut(s)	842, 1135, 1398
BssMI	GATC	4 cut(s)	11, 381, 557, 585
BssNI	GRCGYC	1 cut(s)	102
BssT1I	CCWWGG	1 cut(s)	1398
Bst2UI	CCWGG	2 cut(s)	1137, 1367
Bst4CI	ACNGT	4 cut(s)	145, 174, 377, 734
Bst6I	CTCTTC	5 cut(s)	128, 567, 812, 1257, 1452
BstACI	GRCGYC	1 cut(s)	102
BstAFI	CTTAAG	1 cut(s)	1316
BstBAI	YACGTR	1 cut(s)	669
BstBI	TTCGAA	1 cut(s)	767
BstC8I	GCNNGC	2 cut(s)	390, 472
BstDEI	CTNAG	7 cut(s)	294, 356, 656, 689, 878, 905, 1255
BstEII	GGTNACC	1 cut(s)	522
BstF5I	GGATG	3 cut(s)	248, 760, 1145
BstFNI	CGCG	1 cut(s)	149
BstHHI	GCGC	3 cut(s)	151, 709, 841
BstKTI	GATC	4 cut(s)	14, 384, 560, 588
BstMAI	GTCTC	5 cut(s)	306, 374, 502, 1213, 1447
BstMBI	GATC	4 cut(s)	11, 381, 557, 585
BstMWI	GCNNNNNNNGC	1 cut(s)	748
BstNI	CCWGG	2 cut(s)	1137, 1367
BstPI	GGTNACC	1 cut(s)	522
BstSCI	CCNGG	9 cut(s)	198, 345, 350, 503, 511, 581, 1135, 1365, 1421
BstSLI	GKGCMC	2 cut(s)	352, 1428
BstUI	CGCG	1 cut(s)	149
BstV1I	GCAGC	3 cut(s)	7, 696, 729
BstX2I	RGATCY	1 cut(s)	11
BstYI	RGATCY	1 cut(s)	11
BsuRI	GGCC	3 cut(s)	350, 923, 1397
BtgZI	GCGATG	1 cut(s)	451
BtrI	CACGTC	2 cut(s)	368, 421
BtsCI	GGATG	3 cut(s)	248, 760, 1145
BtsI	GCAGTG	1 cut(s)	1426
BtsIMutI	CAGTG	1 cut(s)	1426
BveI	ACCTGC	1 cut(s)	400
Cac8I	GCNNGC	2 cut(s)	390, 472
CfoI	GCGC	3 cut(s)	151, 709, 841
Cfr13I	GGNCC	4 cut(s)	202, 298, 348, 349
CseI	GACGC	2 cut(s)	110, 148
Csp6I	GTAC	1 cut(s)	808
CviAII	CATG	3 cut(s)	240, 941, 1388
CviQI	GTAC	1 cut(s)	808
DdeI	CTNAG	7 cut(s)	294, 356, 656, 689, 878, 905, 1255
DpnI	GATC	4 cut(s)	13, 383, 559, 587
DpnII	GATC	4 cut(s)	11, 381, 557, 585
DraIII	CACNNNGTG	1 cut(s)	406
Eam1104I	CTCTTC	5 cut(s)	128, 567, 812, 1257, 1452
EarI	CTCTTC	5 cut(s)	128, 567, 812, 1257, 1452
Eco130I	CCWWGG	1 cut(s)	1398
Eco147I	AGGCCT	2 cut(s)	923, 1397
Eco24I	GRGCYC	1 cut(s)	352
Eco31I	GGTCTC	1 cut(s)	502
Eco47I	GGWCC	2 cut(s)	202, 298
Eco57I	CTGAAG	2 cut(s)	548, 1433
Eco72I	CACGTG	1 cut(s)	669
Eco91I	GGTNACC	1 cut(s)	522
EcoO109I	RGGNCCY	1 cut(s)	298
EcoO65I	GGTNACC	1 cut(s)	522
EcoRII	CCWGG	2 cut(s)	1135, 1365
EcoT14I	CCWWGG	1 cut(s)	1398
EcoT38I	GRGCYC	1 cut(s)	352
ErhI	CCWWGG	1 cut(s)	1398
Esp3I	CGTCTC	1 cut(s)	374
FaeI	CATG	3 cut(s)	243, 944, 1391
FalI	AAGNNNNNCTT	4 cut(s)	26, 58, 1289, 1321
FaqI	GGGAC	2 cut(s)	487, 1118
FatI	CATG	3 cut(s)	239, 940, 1387
Fnu4HI	GCNGC	4 cut(s)	18, 21, 710, 743
FokI	GGATG	3 cut(s)	235, 767, 1132
FriOI	GRGCYC	1 cut(s)	352
Fsp4HI	GCNGC	4 cut(s)	18, 21, 710, 743
FspBI	CTAG	3 cut(s)	456, 725, 1437
GlaI	GCGC	3 cut(s)	150, 708, 840
GluI	GCNGC	4 cut(s)	18, 21, 710, 743
HaeIII	GGCC	3 cut(s)	350, 923, 1397
HgaI	GACGC	2 cut(s)	110, 148
HhaI	GCGC	3 cut(s)	151, 709, 841
Hin1I	GRCGYC	1 cut(s)	102
Hin1II	CATG	3 cut(s)	243, 944, 1391
Hin6I	GCGC	3 cut(s)	149, 707, 839
HinP1I	GCGC	3 cut(s)	149, 707, 839
HindIII	AAGCTT	2 cut(s)	35, 962
HinfI	GANTC	9 cut(s)	278, 334, 476, 629, 901, 1039, 1183, 1277, 1330
HphI	GGTGA	3 cut(s)	79, 838, 1304
Hpy166II	GTNNAC	1 cut(s)	1419
Hpy188III	TCNNGA	3 cut(s)	649, 1120, 1156
Hpy8I	GTNNAC	1 cut(s)	1419
Hpy99I	CGWCG	3 cut(s)	104, 149, 422
HpyAV	CCTTC	4 cut(s)	205, 540, 646, 650
HpyCH4III	ACNGT	4 cut(s)	145, 174, 377, 734
HpyCH4IV	ACGT	3 cut(s)	367, 420, 668
HpyF10VI	GCNNNNNNNGC	1 cut(s)	748
HpyF3I	CTNAG	7 cut(s)	294, 356, 656, 689, 878, 905, 1255
HpySE526I	ACGT	3 cut(s)	367, 420, 668
Hsp92I	GRCGYC	1 cut(s)	102
Hsp92II	CATG	3 cut(s)	243, 944, 1391
HspAI	GCGC	3 cut(s)	149, 707, 839
Kzo9I	GATC	4 cut(s)	11, 381, 557, 585
LmnI	GCTCC	1 cut(s)	942
Lsp1109I	GCAGC	3 cut(s)	7, 696, 729
LweI	GCATC	6 cut(s)	93, 694, 826, 1084, 1154, 1318
MaeI	CTAG	3 cut(s)	456, 725, 1437
MaeII	ACGT	3 cut(s)	367, 420, 668
MaeIII	GTNAC	4 cut(s)	255, 522, 908, 1467
MalI	GATC	4 cut(s)	13, 383, 559, 587
MboI	GATC	4 cut(s)	11, 381, 557, 585
MfeI	CAATTG	1 cut(s)	1027
MflI	RGATCY	1 cut(s)	11
MhlI	GDGCHC	3 cut(s)	352, 417, 1428
MluCI	AATT	5 cut(s)	268, 770, 1027, 1054, 1509
MlyI	GAGTC	3 cut(s)	287, 470, 638
MmeI	TCCRAC	1 cut(s)	306
MroXI	GAANNNNTTC	2 cut(s)	734, 1301
MseI	TTAA	5 cut(s)	482, 497, 719, 1197, 1317
MslI	CAYNNNNRTG	2 cut(s)	932, 945
MspA1I	CMGCKG	1 cut(s)	20
MspCI	CTTAAG	1 cut(s)	1316
MspR9I	CCNGG	9 cut(s)	200, 347, 352, 505, 513, 583, 1137, 1367, 1423
MunI	CAATTG	1 cut(s)	1027
MvaI	CCWGG	2 cut(s)	1137, 1367
MvnI	CGCG	1 cut(s)	149
MwoI	GCNNNNNNNGC	1 cut(s)	748
NciI	CCSGG	7 cut(s)	200, 347, 352, 505, 513, 583, 1423
NdeII	GATC	4 cut(s)	11, 381, 557, 585
NlaIII	CATG	3 cut(s)	243, 944, 1391
NlaIV	GGNNCC	3 cut(s)	344, 350, 701
NmeAIII	GCCGAG	1 cut(s)	867
NmuCI	GTSAC	2 cut(s)	255, 1467
NspV	TTCGAA	1 cut(s)	767
PaqCI	CACCTGC	1 cut(s)	400
PceI	AGGCCT	2 cut(s)	923, 1397
PcsI	WCGNNNNNNNCGW	1 cut(s)	188
PdmI	GAANNNNTTC	2 cut(s)	734, 1301
PfeI	GAWTC	6 cut(s)	334, 901, 1039, 1183, 1277, 1330
PfoI	TCCNGGA	3 cut(s)	198, 503, 581
PkrI	GCNGC	4 cut(s)	19, 22, 711, 744
PleI	GAGTC	3 cut(s)	286, 470, 637
PmaCI	CACGTG	1 cut(s)	669
PmlI	CACGTG	1 cut(s)	669
PpsI	GAGTC	3 cut(s)	286, 470, 637
Ppu21I	YACGTR	1 cut(s)	669
PpuMI	RGGWCCY	1 cut(s)	298
Psp5II	RGGWCCY	1 cut(s)	298
Psp6I	CCWGG	2 cut(s)	1135, 1365
PspCI	CACGTG	1 cut(s)	669
PspEI	GGTNACC	1 cut(s)	522
PspGI	CCWGG	2 cut(s)	1135, 1365
PspN4I	GGNNCC	3 cut(s)	344, 350, 701
PspOMI	GGGCCC	1 cut(s)	348
PspPI	GGNCC	4 cut(s)	202, 298, 348, 349
PspPPI	RGGWCCY	1 cut(s)	298
PsuI	RGATCY	1 cut(s)	11
RsaI	GTAC	1 cut(s)	809
RsaNI	GTAC	1 cut(s)	808
RseI	CAYNNNNRTG	2 cut(s)	932, 945
SaqAI	TTAA	5 cut(s)	482, 497, 719, 1197, 1317
SatI	GCNGC	4 cut(s)	18, 21, 710, 743
Sau3AI	GATC	4 cut(s)	11, 381, 557, 585
Sau96I	GGNCC	4 cut(s)	202, 298, 348, 349
SchI	GAGTC	3 cut(s)	287, 470, 638
ScrFI	CCNGG	9 cut(s)	200, 347, 352, 505, 513, 583, 1137, 1367, 1423
SduI	GDGCHC	3 cut(s)	352, 417, 1428
SfaNI	GCATC	6 cut(s)	93, 694, 826, 1084, 1154, 1318
SfuI	TTCGAA	1 cut(s)	767
SinI	GGWCC	2 cut(s)	202, 298
SmiMI	CAYNNNNRTG	2 cut(s)	932, 945
SmlI	CTYRAG	2 cut(s)	647, 1316
SmoI	CTYRAG	2 cut(s)	647, 1316
Sse9I	AATT	5 cut(s)	268, 770, 1027, 1054, 1509
SseBI	AGGCCT	2 cut(s)	923, 1397
SsiI	CCGC	4 cut(s)	18, 82, 549, 784
SspI	AATATT	2 cut(s)	76, 1097
SspMI	CTAG	3 cut(s)	456, 725, 1437
StuI	AGGCCT	2 cut(s)	923, 1397
StyD4I	CCNGG	9 cut(s)	198, 345, 350, 503, 511, 581, 1135, 1365, 1421
StyI	CCWWGG	1 cut(s)	1398
TaaI	ACNGT	4 cut(s)	145, 174, 377, 734
TaiI	ACGT	3 cut(s)	370, 423, 671
TaqI	TCGA	6 cut(s)	207, 417, 663, 738, 767, 1020
TasI	AATT	5 cut(s)	268, 770, 1027, 1054, 1509
TauI	GCSGC	1 cut(s)	20
TfiI	GAWTC	6 cut(s)	334, 901, 1039, 1183, 1277, 1330
Tru1I	TTAA	5 cut(s)	482, 497, 719, 1197, 1317
Tru9I	TTAA	5 cut(s)	482, 497, 719, 1197, 1317
TscAI	CASTG	1 cut(s)	1433
TseFI	GTSAC	2 cut(s)	255, 1467
TseI	GCWGC	3 cut(s)	20, 709, 742
Tsp45I	GTSAC	2 cut(s)	255, 1467
TspDTI	ATGAA	3 cut(s)	72, 957, 1371
TspRI	CASTG	1 cut(s)	1433
Vha464I	CTTAAG	1 cut(s)	1316
VpaK11BI	GGWCC	2 cut(s)	202, 298
XmnI	GAANNNNTTC	2 cut(s)	734, 1301
XspI	CTAG	3 cut(s)	456, 725, 1437

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.