Rorug02G0549000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002693
Physical Location & Seq
Reverse (-)
67647096 .. 67648674
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0549000.1

Sequence Viewer

Length: 936 bp
ATGAGAAGAAGCCTACTCAGACTCAGAGCTTCTTCAGCACCCCATGTCCTTCCTCCATGGCCTCCTCCTTCCTACTACAAAGGTGGGTCTTCATCAAAAGCCTCAAACTTTCAGTCCACGTCAACTTTCAGCTCAAATTCCAGGTGGGTCGTCGTCACCAACTCTCTTCCTCCACCAGAATGGGTAGAGCCTTTCAATGACGTCTCTGACATAGTCTCCAGCCCACAACGCTTCGACCCATCTCCATGGGTCGCCCAAATTCTCTATCTTTTAGACGGGTCTCCACAGATGGAACCCAACTTAGACTCTTACTGCCACAAATTCCTGATCAAACTGTCTCCCAATTTCGTTGCATTTGTGTTGAAATCTGATGATCTTCGAGGAAAGCCCGAGACTACTCTCCGGTTCTTCTCCTGGGTCGGTAAGCAAAAGAAGTATCATCATAAGCTTGAATGCTATGTGTCTTTGATTGAGCTTTTGTGTTTGTGTGGTGATTTGGATAGGATTAGATGTGTTGTTATTGAGCTTAAAAAGATGAACTTTTTGATGAATTCGAATGCGGCGAATTGTTTGATTAAGAGTTTTGGCTGTCTTGGAATGGTTGAGGAGTTGTTGTGGGTTTGGCGTGGGATGAAGGAGAATGGGACTGAGCCTAGTTTGTATACTTATAACTTTTTGGTGAATGGATTGGTGAACTCGATGTTTATTGAATCCGCGGAGCGGGTTTTTGAGGTTATGGAAGGTGGGAAGATTGTGCCTGACATTGTGACTTATAATACAATGATTAAAGGGTATTGTAAGTCAGGGAAAACCCAGAAGGCAATGGAGAAGTTTAGGGATATGGAGGGGAGGAATGTGGAGGCTAATAAGATTACTTATATGACTTTGATGCAGGGGTACGGTGTTATTCGGAAGGAGATTTCGATTCATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

35.45

Weight (kDa)

8.9

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 185 - 232 1.1e-07 PPR repeat family
PPR_2 PF13041 236 - 267 2.6e-07 PPR repeat family
PPR_3 PF13812 242 - 301 1.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 251 - 282 8.1e-13 PPR repeat
PPR_2 PF13041 253 - 300 2.2e-16 PPR repeat family
PPR PF01535 256 - 286 9.8e-10 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 669, 774
AatII GACGTC 1 cut(s) 204
AccBSI CCGCTC 1 cut(s) 721
AccI GTMKAC 1 cut(s) 662
AccII CGCG 1 cut(s) 716
AciI CCGC 4 cut(s) 560, 714, 716, 721
AcsI RAATTY 4 cut(s) 136, 258, 320, 550
AcuI CTGAAG 1 cut(s) 18
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 899
AfiI CCNNNNNNNGG 1 cut(s) 720
AgsI TTSAA 4 cut(s) 196, 364, 452, 710
AjiI CACGTC 1 cut(s) 120
AjnI CCWGG 2 cut(s) 140, 413
AluBI AGCT 5 cut(s) 29, 132, 448, 475, 526
AluI AGCT 5 cut(s) 29, 132, 448, 475, 526
Alw26I GTCTC 5 cut(s) 208, 220, 285, 342, 386
Ama87I CYCGRG 1 cut(s) 389
AoxI GGCC 1 cut(s) 59
ApoI RAATTY 4 cut(s) 136, 258, 320, 550
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
AsuHPI GGTGA 4 cut(s) 148, 503, 691, 703
AsuII TTCGAA 1 cut(s) 554
AvaI CYCGRG 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 81
BccI CCATC 2 cut(s) 247, 283
BciT130I CCWGG 2 cut(s) 142, 415
BclI TGATCA 1 cut(s) 327
BcoDI GTCTC 5 cut(s) 208, 220, 285, 342, 386
BfaI CTAG 1 cut(s) 654
BisI GCNGC 1 cut(s) 561
BlsI GCNGC 1 cut(s) 562
Bme1390I CCNGG 2 cut(s) 142, 415
BmeT110I CYCGRG 1 cut(s) 389
BmgBI CACGTC 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 2 cut(s) 142, 415
BmsI GCATC 1 cut(s) 879
BpiI GAAGAC 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 202
Bpu14I TTCGAA 1 cut(s) 554
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 285
BsaJI CCNNGG 4 cut(s) 56, 245, 414, 714
BsaWI WCCGGW 1 cut(s) 402
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 599, 629
Bsc4I CCNNNNNNNGG 1 cut(s) 720
Bse3DI GCAATG 1 cut(s) 828
BseBI CCWGG 2 cut(s) 142, 415
BseDI CCNNGG 4 cut(s) 56, 245, 414, 714
BseGI GGATG 1 cut(s) 636
BseLI CCNNNNNNNGG 1 cut(s) 720
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 3 cut(s) 31, 37, 639
BseRI GAGGAG 2 cut(s) 54, 620
Bsh1236I CGCG 1 cut(s) 716
BshFI GGCC 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 389
BsiSI CCGG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 1 cut(s) 720
BsmAI GTCTC 5 cut(s) 208, 220, 285, 342, 386
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 658
BsmI GAATGC 2 cut(s) 458, 562
BsnI GGCC 1 cut(s) 61
Bso31I GGTCTC 1 cut(s) 285
BsoBI CYCGRG 1 cut(s) 389
Bsp119I TTCGAA 1 cut(s) 554
Bsp143I GATC 2 cut(s) 327, 373
Bsp19I CCATGG 2 cut(s) 56, 245
BspACI CCGC 4 cut(s) 560, 714, 716, 721
BspANI GGCC 1 cut(s) 61
BspCNI CTCAG 3 cut(s) 30, 36, 640
BspFNI CGCG 1 cut(s) 716
BspLI GGNNCC 1 cut(s) 294
BspT104I TTCGAA 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 285
BsrBI CCGCTC 1 cut(s) 721
BsrDI GCAATG 1 cut(s) 828
BssECI CCNNGG 4 cut(s) 56, 245, 414, 714
BssMI GATC 2 cut(s) 327, 373
BssNAI GTATAC 1 cut(s) 663
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 2 cut(s) 56, 245
Bst1107I GTATAC 1 cut(s) 663
Bst2UI CCWGG 2 cut(s) 142, 415
Bst4CI ACNGT 2 cut(s) 336, 902
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 1 cut(s) 201
BstBI TTCGAA 1 cut(s) 554
BstDEI CTNAG 4 cut(s) 17, 23, 301, 648
BstDSI CCRYGG 3 cut(s) 56, 245, 714
BstF5I GGATG 1 cut(s) 636
BstFNI CGCG 1 cut(s) 716
BstKTI GATC 2 cut(s) 330, 376
BstMAI GTCTC 5 cut(s) 208, 220, 285, 342, 386
BstMBI GATC 2 cut(s) 327, 373
BstMWI GCNNNNNNNGC 2 cut(s) 35, 228
BstNI CCWGG 2 cut(s) 142, 415
BstSCI CCNGG 2 cut(s) 140, 413
BstUI CGCG 1 cut(s) 716
BstV2I GAAGAC 1 cut(s) 81
BstXI CCANNNNNNTGG 2 cut(s) 180, 246
BstZ17I GTATAC 1 cut(s) 663
BsuRI GGCC 1 cut(s) 61
BtgI CCRYGG 3 cut(s) 56, 245, 714
BtrI CACGTC 1 cut(s) 120
BtsCI GGATG 1 cut(s) 636
Cfr42I CCGCGG 1 cut(s) 717
Csp6I GTAC 1 cut(s) 898
CviAII CATG 4 cut(s) 44, 57, 246, 929
CviQI GTAC 1 cut(s) 898
DdeI CTNAG 4 cut(s) 17, 23, 301, 648
DpnI GATC 2 cut(s) 329, 375
DpnII GATC 2 cut(s) 327, 373
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 56, 245
Eco31I GGTCTC 1 cut(s) 285
Eco57I CTGAAG 1 cut(s) 18
Eco88I CYCGRG 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 550
EcoRII CCWGG 2 cut(s) 140, 413
EcoT14I CCWWGG 2 cut(s) 56, 245
ErhI CCWWGG 2 cut(s) 56, 245
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 4 cut(s) 47, 60, 249, 932
FalI AAGNNNNNCTT 2 cut(s) 524, 556
FaqI GGGAC 1 cut(s) 658
FatI CATG 4 cut(s) 43, 56, 245, 928
FauI CCCGC 1 cut(s) 714
FbaI TGATCA 1 cut(s) 327
FblI GTMKAC 1 cut(s) 662
Fnu4HI GCNGC 1 cut(s) 561
FokI GGATG 1 cut(s) 643
Fsp4HI GCNGC 1 cut(s) 561
FspBI CTAG 1 cut(s) 654
GluI GCNGC 1 cut(s) 561
GsuI CTGGAG 1 cut(s) 202
HaeIII GGCC 1 cut(s) 61
HapII CCGG 1 cut(s) 403
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 4 cut(s) 47, 60, 249, 932
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 4 cut(s) 21, 305, 710, 925
HpaII CCGG 1 cut(s) 403
HphI GGTGA 4 cut(s) 148, 503, 691, 703
Hpy166II GTNNAC 4 cut(s) 117, 123, 663, 694
Hpy188I TCNGA 5 cut(s) 20, 26, 208, 370, 912
Hpy188III TCNNGA 1 cut(s) 325
Hpy8I GTNNAC 4 cut(s) 117, 123, 663, 694
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 6 cut(s) 59, 78, 628, 734, 811, 907
HpyCH4III ACNGT 2 cut(s) 336, 902
HpyCH4IV ACGT 2 cut(s) 119, 201
HpyCH4V TGCA 2 cut(s) 353, 892
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 228
HpyF3I CTNAG 4 cut(s) 17, 23, 301, 648
HpySE526I ACGT 2 cut(s) 119, 201
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 4 cut(s) 47, 60, 249, 932
Ksp22I TGATCA 1 cut(s) 327
KspI CCGCGG 1 cut(s) 717
Kzo9I GATC 2 cut(s) 327, 373
LmnI GCTCC 1 cut(s) 718
LweI GCATC 1 cut(s) 879
MaeI CTAG 1 cut(s) 654
MaeII ACGT 2 cut(s) 119, 201
MaeIII GTNAC 2 cut(s) 154, 766
MalI GATC 2 cut(s) 329, 375
MbiI CCGCTC 1 cut(s) 721
MboI GATC 2 cut(s) 327, 373
MboII GAAGA 7 cut(s) 18, 24, 81, 158, 368, 400, 760
MluCI AATT 6 cut(s) 136, 258, 320, 343, 550, 565
MlyI GAGTC 2 cut(s) 15, 299
MseI TTAA 3 cut(s) 528, 576, 786
MslI CAYNNNNRTG 2 cut(s) 178, 244
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 2 cut(s) 142, 415
Mva1269I GAATGC 2 cut(s) 458, 562
MvaI CCWGG 2 cut(s) 142, 415
MvnI CGCG 1 cut(s) 716
MwoI GCNNNNNNNGC 2 cut(s) 35, 228
NcoI CCATGG 2 cut(s) 56, 245
NdeII GATC 2 cut(s) 327, 373
NlaIII CATG 4 cut(s) 47, 60, 249, 932
NlaIV GGNNCC 1 cut(s) 294
NmuCI GTSAC 2 cut(s) 154, 766
NspV TTCGAA 1 cut(s) 554
PcsI WCGNNNNNNNCGW 1 cut(s) 560
PctI GAATGC 2 cut(s) 458, 562
PfeI GAWTC 2 cut(s) 710, 925
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 1 cut(s) 562
PleI GAGTC 2 cut(s) 15, 299
PpsI GAGTC 2 cut(s) 15, 299
PsiI TTATAA 2 cut(s) 669, 774
Psp6I CCWGG 2 cut(s) 140, 413
PspGI CCWGG 2 cut(s) 140, 413
PspN4I GGNNCC 1 cut(s) 294
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 1 cut(s) 899
RsaNI GTAC 1 cut(s) 898
RseI CAYNNNNRTG 2 cut(s) 178, 244
SacII CCGCGG 1 cut(s) 717
SaqAI TTAA 3 cut(s) 528, 576, 786
SatI GCNGC 1 cut(s) 561
Sau3AI GATC 2 cut(s) 327, 373
SchI GAGTC 2 cut(s) 15, 299
ScrFI CCNGG 2 cut(s) 142, 415
SfaNI GCATC 1 cut(s) 879
Sfr303I CCGCGG 1 cut(s) 717
SfuI TTCGAA 1 cut(s) 554
SgrBI CCGCGG 1 cut(s) 717
SmiMI CAYNNNNRTG 2 cut(s) 178, 244
Sse9I AATT 6 cut(s) 136, 258, 320, 343, 550, 565
SsiI CCGC 4 cut(s) 560, 714, 716, 721
SspMI CTAG 1 cut(s) 654
StyD4I CCNGG 2 cut(s) 140, 413
StyI CCWWGG 2 cut(s) 56, 245
TaaI ACNGT 2 cut(s) 336, 902
TaiI ACGT 2 cut(s) 122, 204
TaqI TCGA 5 cut(s) 234, 379, 554, 698, 923
TasI AATT 6 cut(s) 136, 258, 320, 343, 550, 565
TauI GCSGC 1 cut(s) 563
TfiI GAWTC 2 cut(s) 710, 925
Tru1I TTAA 3 cut(s) 528, 576, 786
Tru9I TTAA 3 cut(s) 528, 576, 786
TseFI GTSAC 2 cut(s) 154, 766
Tsp45I GTSAC 2 cut(s) 154, 766
TspDTI ATGAA 5 cut(s) 81, 551, 563, 647, 917
Tth111I GACNNNGTC 1 cut(s) 212
XapI RAATTY 4 cut(s) 136, 258, 320, 550
XmiI GTMKAC 1 cut(s) 662
XspI CTAG 1 cut(s) 654
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.