Rorug03G0056200

BAG family molecular chaperone regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002445
Physical Location & Seq
Forward (+)
4291841 .. 4292759
919 bp
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UTR
Exon/CDS
Intron
Rorug03G0056200.1

Sequence Viewer

Length: 582 bp
ATGGCCTTCTCAAAACTGAGCTTGTTTGCCTTTGTGCTTGTTCACGCCTTCCTCCTCCATTCTTCTCCAGTATATTGTAATGAAGAAGAAGACGATCTTTTCGAGGAACTCAACAGTTTCAGGGGATCCCTAAACCTCACAGCCTTCACAGAAAACGAAAAGGCCGATTGTATAGCCGACGAAATCTCAGATGAGCTAGAGGATGAGCTATGCTCTAGAGCAGCTGAATACCAGATTAAACCAGGAAATGGTCCTCAGTTCCCCTCCTTCACCAAGTCCTTAACCAAGTGTGACATAGACAACAGCACCACAACTGATGGGATCATTTTGCCTGTTTGTGTGCGCAATTTGGACCCAGATCTGGTGCGTACAAATTATACTGACACCAAATTTGCCCACTTTCTGAATGATTCCAAGTATACAGGAGCTGGGCTTGGTTCTAAGGATGATTGGATGATCGTTGTGTTGACCACTGACGCACATAGTGGGAGCTTTACTGGTGGTGCTGCTTCTTTGGTTGCAACTGGCATGGTTCACTACATGGTTGCTTTGTTGTTGGGATTGTTCTTGGTTTTGCTATGA

Protein Analysis

193

Amino Acids

21.15

Weight (kDa)

4.42

Isoelectric Point (pI)

27.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 30 - 156 9.1e-51 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 344
AccB7I CCANNNNNTGG 1 cut(s) 248
AccI GTMKAC 1 cut(s) 419
AclWI GGATC 3 cut(s) 120, 133, 329
AcsI RAATTY 1 cut(s) 389
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 1 cut(s) 370
AfiI CCNNNNNNNGG 2 cut(s) 248, 361
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 6 cut(s) 21, 196, 208, 224, 428, 492
AluI AGCT 6 cut(s) 21, 196, 208, 224, 428, 492
AlwI GGATC 3 cut(s) 120, 133, 329
AlwNI CAGNNNCTG 1 cut(s) 428
AoxI GGCC 2 cut(s) 3, 162
ApeKI GCWGC 2 cut(s) 221, 506
ApoI RAATTY 1 cut(s) 389
ArsI GACNNNNNNTTYG 4 cut(s) 83, 115, 374, 406
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 2 cut(s) 251, 352
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 2 cut(s) 251, 352
BamHI GGATCC 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 233, 493
BccI CCATC 1 cut(s) 311
BciT130I CCWGG 1 cut(s) 243
BfaI CTAG 2 cut(s) 197, 216
BglII AGATCT 1 cut(s) 358
BisI GCNGC 2 cut(s) 222, 507
BlsI GCNGC 2 cut(s) 223, 508
Bme1390I CCNGG 1 cut(s) 243
Bme18I GGWCC 2 cut(s) 251, 352
BmgT120I GGNCC 2 cut(s) 251, 352
BmiI GGNNCC 2 cut(s) 127, 354
BmrFI CCNGG 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 96
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpmI CTGGAG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 361
Bse1I ACTGG 3 cut(s) 68, 502, 529
BseBI CCWGG 1 cut(s) 243
BseGI GGATG 3 cut(s) 208, 451, 459
BseLI CCNNNNNNNGG 2 cut(s) 248, 361
BseMII CTCAG 3 cut(s) 8, 201, 269
BseNI ACTGG 3 cut(s) 68, 502, 529
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 2 cut(s) 233, 493
BseYI CCCAGC 1 cut(s) 428
BshFI GGCC 2 cut(s) 5, 164
BslI CCNNNNNNNGG 2 cut(s) 248, 361
BsnI GGCC 2 cut(s) 5, 164
Bsp143I GATC 5 cut(s) 94, 125, 321, 358, 456
BspANI GGCC 2 cut(s) 5, 164
BspCNI CTCAG 3 cut(s) 9, 200, 268
BspLI GGNNCC 2 cut(s) 127, 354
BspPI GGATC 3 cut(s) 120, 133, 329
BsrI ACTGG 3 cut(s) 68, 502, 529
BssMI GATC 5 cut(s) 94, 125, 321, 358, 456
BssNAI GTATAC 1 cut(s) 420
Bst1107I GTATAC 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 116
BstDEI CTNAG 4 cut(s) 17, 187, 255, 441
BstF5I GGATG 3 cut(s) 208, 451, 459
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 5 cut(s) 97, 128, 324, 361, 459
BstMBI GATC 5 cut(s) 94, 125, 321, 358, 456
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 2 cut(s) 233, 493
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 2 cut(s) 125, 358
BstYI RGATCY 2 cut(s) 125, 358
BstZ17I GTATAC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 5, 164
BtsCI GGATG 3 cut(s) 208, 451, 459
BtsIMutI CAGTG 1 cut(s) 471
CaiI CAGNNNCTG 1 cut(s) 428
CfoI GCGC 1 cut(s) 345
Cfr13I GGNCC 2 cut(s) 251, 352
CseI GACGC 1 cut(s) 485
Csp6I GTAC 1 cut(s) 369
CviAII CATG 2 cut(s) 529, 541
CviQI GTAC 1 cut(s) 369
DdeI CTNAG 4 cut(s) 17, 187, 255, 441
DpnI GATC 5 cut(s) 96, 127, 323, 360, 458
DpnII GATC 5 cut(s) 94, 125, 321, 358, 456
DraIII CACNNNGTG 1 cut(s) 485
Eco47I GGWCC 2 cut(s) 251, 352
EcoRII CCWGG 1 cut(s) 241
FaeI CATG 2 cut(s) 532, 544
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FatI CATG 2 cut(s) 528, 540
FblI GTMKAC 1 cut(s) 419
Fnu4HI GCNGC 2 cut(s) 222, 507
FokI GGATG 3 cut(s) 215, 458, 466
Fsp4HI GCNGC 2 cut(s) 222, 507
FspBI CTAG 2 cut(s) 197, 216
FspI TGCGCA 1 cut(s) 344
GlaI GCGC 1 cut(s) 344
GluI GCNGC 2 cut(s) 222, 507
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 1 cut(s) 51
HaeIII GGCC 2 cut(s) 5, 164
HgaI GACGC 1 cut(s) 485
HhaI GCGC 1 cut(s) 345
Hin1II CATG 2 cut(s) 532, 544
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HinfI GANTC 1 cut(s) 410
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 4 cut(s) 43, 420, 468, 535
Hpy188I TCNGA 2 cut(s) 190, 405
Hpy188III TCNNGA 1 cut(s) 216
Hpy8I GTNNAC 4 cut(s) 43, 420, 468, 535
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 4 cut(s) 16, 58, 154, 277
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4V TGCA 1 cut(s) 521
HpyF3I CTNAG 4 cut(s) 17, 187, 255, 441
Hsp92II CATG 2 cut(s) 532, 544
HspAI GCGC 1 cut(s) 343
Kzo9I GATC 5 cut(s) 94, 125, 321, 358, 456
LmnI GCTCC 2 cut(s) 425, 489
Lsp1109I GCAGC 2 cut(s) 233, 493
MaeI CTAG 2 cut(s) 197, 216
MaeIII GTNAC 1 cut(s) 290
MalI GATC 5 cut(s) 96, 127, 323, 360, 458
MboI GATC 5 cut(s) 94, 125, 321, 358, 456
MboII GAAGA 4 cut(s) 54, 95, 98, 101
MflI RGATCY 2 cut(s) 125, 358
MluCI AATT 3 cut(s) 346, 373, 389
MnlI CCTC 7 cut(s) 62, 65, 97, 146, 193, 264, 274
MseI TTAA 2 cut(s) 237, 281
MspA1I CMGCKG 1 cut(s) 224
MspR9I CCNGG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 243
NdeII GATC 5 cut(s) 94, 125, 321, 358, 456
NlaIII CATG 2 cut(s) 532, 544
NlaIV GGNNCC 2 cut(s) 127, 354
NmuCI GTSAC 1 cut(s) 290
NsbI TGCGCA 1 cut(s) 344
PcsI WCGNNNNNNNCGW 2 cut(s) 99, 162
PfeI GAWTC 1 cut(s) 410
PflMI CCANNNNNTGG 1 cut(s) 248
PkrI GCNGC 2 cut(s) 223, 508
Psp6I CCWGG 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 127, 354
PspPI GGNCC 2 cut(s) 251, 352
PstNI CAGNNNCTG 1 cut(s) 428
PsuI RGATCY 2 cut(s) 125, 358
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
SaqAI TTAA 2 cut(s) 237, 281
SatI GCNGC 2 cut(s) 222, 507
Sau3AI GATC 5 cut(s) 94, 125, 321, 358, 456
Sau96I GGNCC 2 cut(s) 251, 352
ScrFI CCNGG 1 cut(s) 243
SetI ASST 7 cut(s) 23, 138, 198, 210, 226, 430, 494
SinI GGWCC 2 cut(s) 251, 352
Sse9I AATT 3 cut(s) 346, 373, 389
SspMI CTAG 2 cut(s) 197, 216
StyD4I CCNGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 116
TaqI TCGA 1 cut(s) 102
TasI AATT 3 cut(s) 346, 373, 389
TfiI GAWTC 1 cut(s) 410
Tru1I TTAA 2 cut(s) 237, 281
Tru9I TTAA 2 cut(s) 237, 281
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 2 cut(s) 221, 506
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 96
TspRI CASTG 1 cut(s) 478
Van91I CCANNNNNTGG 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 251, 352
XapI RAATTY 1 cut(s) 389
XbaI TCTAGA 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 419
XspI CTAG 2 cut(s) 197, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.