Rh5BG480100

BAG family molecular chaperone regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002445
Physical Location & Seq
Reverse (-)
76375086 .. 76378439
3354 bp
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UTR
Exon/CDS
Intron
Rh5BG480100.1

Sequence Viewer

Length: 609 bp
ATGAAAGGCGATCTGAAGAGAATTCTTGCAAATGAGACTGGATTAGAGCCAAAGGCGCAGAGGTTGTTGTATAGAGGAAAAGAGAAGGATGATGTTGAATGTTTGCACATCGCAGGTGTAAAAGATATGTCTAAGATTATGTTATTGGAAGACCCAGCTAGCAAAGAGAAGAAGCTTGAAGAAATGAACAAAAATCAAGGTATACTGAAAGCCTATGAAGAGGTTGCCAAAGTTAGAGCACAAGTTGACAAGCTCTCTCAAAAGATTGTGGATATGGAGACAACACTATGCAATGGTACCAAGGTCGCAGATACGGAGATTGTGGTCTTAACAGAGTTGCTCATGGTGGAGTTGCTCAAATTGGATTCCATTGAGGCAGATGGAGAAGCAAAAGTACAGAGGCGGGTAGAGGTTCGTCGTGTACAAAGTTTCGTTGATACACTCGACAACCTAAAAGCAAGAAACTCTAATCCATTCAGCAAGAGTAGCAACTCTGTCTCAGTGACTACAAATTGGGAGAGTTTTGAATTGGGGGTTGGAAGCCTGCATGCCCCAACCCCATTGAAATCTTTTACCAAGATAACTCAAGATTGGGAACTGTTTGACTAA

Protein Analysis

202

Amino Acids

22.75

Weight (kDa)

5.63

Isoelectric Point (pI)

28.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAG PF02179 75 - 151 7.6e-15 BAG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 104
Acc65I GGTACC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 296
AccI GTMKAC 1 cut(s) 202
AciI CCGC 1 cut(s) 403
AcsI RAATTY 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 35
AfaI GTAC 3 cut(s) 298, 396, 423
AgsI TTSAA 4 cut(s) 98, 179, 527, 565
AjuI GAANNNNNNNTTGG 2 cut(s) 519, 551
AluBI AGCT 3 cut(s) 158, 175, 253
AluI AGCT 3 cut(s) 158, 175, 253
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 3 cut(s) 29, 272, 502
ApoI RAATTY 1 cut(s) 21
Asp718I GGTACC 1 cut(s) 296
AspLEI GCGC 1 cut(s) 58
AsuNHI GCTAGC 1 cut(s) 158
BaeI ACNNNNGTAYC 2 cut(s) 288, 321
BanI GGYRCC 1 cut(s) 296
BarI GAAGNNNNNNTAC 2 cut(s) 378, 410
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 241
BccI CCATC 1 cut(s) 374
BcoDI GTCTC 3 cut(s) 29, 272, 502
BfaI CTAG 1 cut(s) 159
BfuAI ACCTGC 1 cut(s) 104
BmiI GGNNCC 1 cut(s) 298
BmtI GCTAGC 1 cut(s) 162
BpiI GAAGAC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 300
Bse1I ACTGG 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 43
BseYI CCCAGC 1 cut(s) 154
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 1 cut(s) 241
BsmAI GTCTC 3 cut(s) 29, 272, 502
Bsp1286I GDGCHC 1 cut(s) 241
Bsp1407I TGTACA 1 cut(s) 421
Bsp143I GATC 1 cut(s) 10
BspACI CCGC 1 cut(s) 403
BspCNI CTCAG 1 cut(s) 512
BspLI GGNNCC 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 104
BspOI GCTAGC 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 298
BsrGI TGTACA 1 cut(s) 421
BsrI ACTGG 1 cut(s) 43
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 1 cut(s) 10
BssNAI GTATAC 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 300
Bst1107I GTATAC 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 600
Bst6I CTCTTC 2 cut(s) 11, 213
BstAUI TGTACA 1 cut(s) 421
BstC8I GCNNGC 3 cut(s) 160, 545, 549
BstDEI CTNAG 2 cut(s) 132, 499
BstF5I GGATG 1 cut(s) 94
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 1 cut(s) 13
BstMAI GTCTC 3 cut(s) 29, 272, 502
BstMBI GATC 1 cut(s) 10
BstMWI GCNNNNNNNGC 2 cut(s) 55, 486
BstNSI RCATGY 1 cut(s) 551
BstV2I GAAGAC 1 cut(s) 156
BstZ17I GTATAC 1 cut(s) 203
BtgZI GCGATG 1 cut(s) 94
BtsCI GGATG 1 cut(s) 94
BtsIMutI CAGTG 1 cut(s) 507
BveI ACCTGC 1 cut(s) 104
Cac8I GCNNGC 3 cut(s) 160, 545, 549
CfoI GCGC 1 cut(s) 58
Csp6I GTAC 3 cut(s) 297, 395, 422
CviAII CATG 2 cut(s) 343, 548
CviJI RGCY 6 cut(s) 49, 158, 175, 212, 253, 543
CviKI_1 RGCY 6 cut(s) 49, 158, 175, 212, 253, 543
CviQI GTAC 3 cut(s) 297, 395, 422
DdeI CTNAG 2 cut(s) 132, 499
DpnI GATC 1 cut(s) 12
DpnII GATC 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 11, 213
EarI CTCTTC 2 cut(s) 11, 213
Eco130I CCWWGG 1 cut(s) 300
Eco57I CTGAAG 1 cut(s) 35
EcoRI GAATTC 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 2 cut(s) 346, 551
FaiI YATR 9 cut(s) 72, 128, 140, 203, 216, 275, 289, 344, 549
FatI CATG 2 cut(s) 342, 547
FauI CCCGC 1 cut(s) 396
FblI GTMKAC 1 cut(s) 202
FokI GGATG 1 cut(s) 101
FspBI CTAG 1 cut(s) 159
GlaI GCGC 1 cut(s) 57
GsaI CCCAGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 58
Hin1II CATG 2 cut(s) 346, 551
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 1 cut(s) 365
Hpy166II GTNNAC 3 cut(s) 203, 247, 422
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 1 cut(s) 587
Hpy8I GTNNAC 3 cut(s) 203, 247, 422
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 1 cut(s) 600
HpyCH4V TGCA 4 cut(s) 29, 106, 291, 547
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 486
HpyF3I CTNAG 2 cut(s) 132, 499
Hsp92II CATG 2 cut(s) 346, 551
HspAI GCGC 1 cut(s) 56
KpnI GGTACC 1 cut(s) 300
Kzo9I GATC 1 cut(s) 10
LpnPI CCDG 4 cut(s) 24, 99, 168, 557
MaeI CTAG 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 502
MalI GATC 1 cut(s) 12
MboI GATC 1 cut(s) 10
MboII GAAGA 5 cut(s) 28, 161, 181, 191, 230
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 4 cut(s) 21, 359, 511, 527
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 6 cut(s) 54, 68, 214, 367, 393, 403
MseI TTAA 1 cut(s) 329
MwoI GCNNNNNNNGC 2 cut(s) 55, 486
NdeII GATC 1 cut(s) 10
NheI GCTAGC 1 cut(s) 158
NlaIII CATG 2 cut(s) 346, 551
NlaIV GGNNCC 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 502
NspI RCATGY 1 cut(s) 551
PaeI GCATGC 1 cut(s) 551
PaqCI CACCTGC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 365
PspFI CCCAGC 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 298
RsaI GTAC 3 cut(s) 298, 396, 423
RsaNI GTAC 3 cut(s) 297, 395, 422
SaqAI TTAA 1 cut(s) 329
Sau3AI GATC 1 cut(s) 10
SduI GDGCHC 1 cut(s) 241
SmlI CTYRAG 1 cut(s) 585
SmoI CTYRAG 1 cut(s) 585
SphI GCATGC 1 cut(s) 551
Sse9I AATT 4 cut(s) 21, 359, 511, 527
SsiI CCGC 1 cut(s) 403
SspMI CTAG 1 cut(s) 159
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 600
TaqI TCGA 1 cut(s) 444
TasI AATT 4 cut(s) 21, 359, 511, 527
TatI WGTACW 2 cut(s) 394, 421
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TscAI CASTG 1 cut(s) 507
TseFI GTSAC 1 cut(s) 502
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 3 cut(s) 17, 200, 231
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 1 cut(s) 507
XapI RAATTY 1 cut(s) 21
XceI RCATGY 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 202
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.