Rorug05G0519500

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001469
Physical Location & Seq
Forward (+)
70375222 .. 70376888
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0519500.1

Sequence Viewer

Length: 519 bp
ATGGACGATGACGTGTCCGCCCCCTTCCGCCGATCCTCGAGTCGGAATCGTAAGGTCGCTTCGAAAATGGCTGCTGCCCTTATGAGCACTGATAACCGCACCCAGGCTGCCCTTGCTAGGCTTGAAGCTTTGGAAAACGACAATGCTGGGTTGGAAACTGTGGAGGTTAATGACGACGATGAAGCTTCTCTTGATGATGAGGATCAAGGATATATACAAAAGAAGCAGCCTAAGGGCACCAAGCGTAAAACCCGGCAGGCAAAAGCTCTTGAGGCCAGGAAGGCACCCAGAACATTCCTTGAGCTTTTACATGAGGCAAATCTTGAATCTTTGCCACCTCATGTCCCCTCCTATCTGAAAGCAGCAGTGGGACCTCCAAGCTCTACCTCCCGCCGCCATTTCTGCACTGTTTGTGGTTCGGCTGCCAACTATACATGTGTGAGGTGTGGTATGCGCTTCTGTTCGTGTCGTTGCCAGAATATACACAATGATACTCGTTGTCAGAAATTTGTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

172

Amino Acids

19.08

Weight (kDa)

8.81

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 131 - 159 8.8e-10 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 236, 283
AciI CCGC 5 cut(s) 18, 28, 97, 391, 394
AclWI GGATC 2 cut(s) 27, 210
AcsI RAATTY 1 cut(s) 506
AfiI CCNNNNNNNGG 3 cut(s) 42, 103, 117
AflIII ACRYGT 2 cut(s) 12, 434
AgsI TTSAA 2 cut(s) 125, 326
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 2 cut(s) 102, 275
AluBI AGCT 5 cut(s) 128, 185, 266, 304, 381
AluI AGCT 5 cut(s) 128, 185, 266, 304, 381
Alw21I GWGCWC 1 cut(s) 89
AlwI GGATC 2 cut(s) 27, 210
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 6 cut(s) 71, 74, 107, 226, 362, 422
ApoI RAATTY 1 cut(s) 506
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 1 cut(s) 253
AsuII TTCGAA 1 cut(s) 62
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 1 cut(s) 371
AxyI CCTNAGG 1 cut(s) 231
BaeGI GKGCMC 1 cut(s) 239
BanI GGYRCC 2 cut(s) 236, 283
BarI GAAGNNNNNNTAC 2 cut(s) 43, 75
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 6 cut(s) 58, 61, 94, 238, 374, 409
BciT130I CCWGG 2 cut(s) 104, 277
BcnI CCSGG 1 cut(s) 253
BfaI CTAG 1 cut(s) 117
BglI GCCNNNNNGGC 1 cut(s) 281
BisI GCNGC 7 cut(s) 72, 75, 108, 227, 363, 394, 423
BlsI GCNGC 7 cut(s) 73, 76, 109, 228, 364, 395, 424
Bme1390I CCNGG 3 cut(s) 104, 253, 277
Bme18I GGWCC 1 cut(s) 371
BmeT110I CYCGRG 1 cut(s) 37
BmgBI CACGTC 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 3 cut(s) 238, 285, 372
BmrFI CCNGG 3 cut(s) 104, 253, 277
Bpu14I TTCGAA 1 cut(s) 62
BpuEI CTTGAG 2 cut(s) 290, 320
BpuMI CCSGG 1 cut(s) 253
BsaBI GATNNNNATC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 102
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 103, 117
Bse21I CCTNAGG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 2 cut(s) 104, 277
BseDI CCNNGG 1 cut(s) 102
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 3 cut(s) 42, 103, 117
BseSI GKGCMC 1 cut(s) 239
BseXI GCAGC 6 cut(s) 58, 61, 94, 238, 374, 409
BseYI CCCAGC 1 cut(s) 146
BsgI GTGCAG 1 cut(s) 388
BshFI GGCC 1 cut(s) 275
BshNI GGYRCC 2 cut(s) 236, 283
BsiHKAI GWGCWC 1 cut(s) 89
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 1 cut(s) 253
BslFI GGGAC 2 cut(s) 329, 384
BslI CCNNNNNNNGG 3 cut(s) 42, 103, 117
BsmFI GGGAC 2 cut(s) 329, 384
BsnI GGCC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 37
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 2 cut(s) 89, 239
Bsp143I GATC 2 cut(s) 32, 202
BspACI CCGC 5 cut(s) 18, 28, 97, 391, 394
BspANI GGCC 1 cut(s) 275
BspLI GGNNCC 3 cut(s) 238, 285, 372
BspPI GGATC 2 cut(s) 27, 210
BspT104I TTCGAA 1 cut(s) 62
BspT107I GGYRCC 2 cut(s) 236, 283
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 2 cut(s) 32, 202
Bst2UI CCWGG 2 cut(s) 104, 277
Bst4CI ACNGT 2 cut(s) 160, 409
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 1 cut(s) 231
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 2 cut(s) 35, 205
BstMBI GATC 2 cut(s) 32, 202
BstMWI GCNNNNNNNGC 4 cut(s) 113, 272, 281, 402
BstNI CCWGG 2 cut(s) 104, 277
BstNSI RCATGY 1 cut(s) 438
BstSCI CCNGG 3 cut(s) 102, 251, 275
BstSLI GKGCMC 1 cut(s) 239
BstV1I GCAGC 6 cut(s) 58, 61, 94, 238, 374, 409
Bsu36I CCTNAGG 1 cut(s) 231
BsuRI GGCC 1 cut(s) 275
BtrI CACGTC 1 cut(s) 13
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 3 cut(s) 87, 372, 405
Cac8I GCNNGC 1 cut(s) 258
CfoI GCGC 1 cut(s) 456
Cfr13I GGNCC 1 cut(s) 371
CviAII CATG 3 cut(s) 311, 341, 435
DdeI CTNAG 1 cut(s) 231
DpnI GATC 2 cut(s) 34, 204
DpnII GATC 2 cut(s) 32, 202
EciI GGCGGA 2 cut(s) 7, 17
Eco47I GGWCC 1 cut(s) 371
Eco81I CCTNAGG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 102, 275
FaeI CATG 3 cut(s) 314, 344, 438
FaiI YATR 9 cut(s) 83, 213, 215, 312, 342, 432, 436, 452, 482
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 2 cut(s) 329, 384
FatI CATG 3 cut(s) 310, 340, 434
FauI CCCGC 1 cut(s) 398
Fnu4HI GCNGC 7 cut(s) 72, 75, 108, 227, 363, 394, 423
Fsp4HI GCNGC 7 cut(s) 72, 75, 108, 227, 363, 394, 423
FspBI CTAG 1 cut(s) 117
GlaI GCGC 1 cut(s) 455
GluI GCNGC 7 cut(s) 72, 75, 108, 227, 363, 394, 423
GsaI CCCAGC 1 cut(s) 150
HaeIII GGCC 1 cut(s) 275
HapII CCGG 1 cut(s) 253
HhaI GCGC 1 cut(s) 456
Hin1II CATG 3 cut(s) 314, 344, 438
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HindIII AAGCTT 2 cut(s) 126, 183
HinfI GANTC 3 cut(s) 40, 46, 326
HpaII CCGG 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 45, 357, 504
Hpy188III TCNNGA 3 cut(s) 191, 269, 323
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 2 cut(s) 34, 274
HpyCH4III ACNGT 2 cut(s) 160, 409
HpyCH4IV ACGT 1 cut(s) 12
HpyCH4V TGCA 1 cut(s) 405
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 272, 281, 402
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 3 cut(s) 314, 344, 438
HspAI GCGC 1 cut(s) 454
Kzo9I GATC 2 cut(s) 32, 202
LpnPI CCDG 9 cut(s) 89, 116, 132, 242, 262, 266, 289, 301, 488
Lsp1109I GCAGC 6 cut(s) 58, 61, 94, 238, 374, 409
MaeI CTAG 1 cut(s) 117
MaeII ACGT 1 cut(s) 12
MalI GATC 2 cut(s) 34, 204
MboI GATC 2 cut(s) 32, 202
MhlI GDGCHC 2 cut(s) 89, 239
MluCI AATT 1 cut(s) 506
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 2 cut(s) 23, 132
MseI TTAA 1 cut(s) 168
MspI CCGG 1 cut(s) 253
MspR9I CCNGG 3 cut(s) 104, 253, 277
MvaI CCWGG 2 cut(s) 104, 277
MwoI GCNNNNNNNGC 4 cut(s) 113, 272, 281, 402
NciI CCSGG 1 cut(s) 253
NdeII GATC 2 cut(s) 32, 202
NlaIII CATG 3 cut(s) 314, 344, 438
NlaIV GGNNCC 3 cut(s) 238, 285, 372
NspI RCATGY 1 cut(s) 438
NspV TTCGAA 1 cut(s) 62
PaeR7I CTCGAG 1 cut(s) 37
PciI ACATGT 1 cut(s) 434
PfeI GAWTC 2 cut(s) 46, 326
PkrI GCNGC 7 cut(s) 73, 76, 109, 228, 364, 395, 424
PleI GAGTC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 48
PpuMI RGGWCCY 1 cut(s) 371
PscI ACATGT 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 371
Psp6I CCWGG 2 cut(s) 102, 275
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 2 cut(s) 102, 275
PspN4I GGNNCC 3 cut(s) 238, 285, 372
PspPI GGNCC 1 cut(s) 371
PspPPI RGGWCCY 1 cut(s) 371
PspXI VCTCGAGB 1 cut(s) 37
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 7 cut(s) 72, 75, 108, 227, 363, 394, 423
Sau3AI GATC 2 cut(s) 32, 202
Sau96I GGNCC 1 cut(s) 371
SchI GAGTC 1 cut(s) 49
ScrFI CCNGG 3 cut(s) 104, 253, 277
SduI GDGCHC 2 cut(s) 89, 239
Sfr274I CTCGAG 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 62
SinI GGWCC 1 cut(s) 371
SlaI CTCGAG 1 cut(s) 37
SmlI CTYRAG 3 cut(s) 37, 269, 299
SmoI CTYRAG 3 cut(s) 37, 269, 299
Sse9I AATT 1 cut(s) 506
SsiI CCGC 5 cut(s) 18, 28, 97, 391, 394
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 3 cut(s) 102, 251, 275
TaaI ACNGT 2 cut(s) 160, 409
TaiI ACGT 1 cut(s) 15
TaqI TCGA 2 cut(s) 38, 62
TasI AATT 1 cut(s) 506
TauI GCSGC 1 cut(s) 396
TfiI GAWTC 2 cut(s) 46, 326
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 3 cut(s) 94, 372, 412
TseI GCWGC 6 cut(s) 71, 74, 107, 226, 362, 422
TspDTI ATGAA 1 cut(s) 195
TspRI CASTG 3 cut(s) 94, 372, 412
VpaK11BI GGWCC 1 cut(s) 371
XapI RAATTY 1 cut(s) 506
XceI RCATGY 1 cut(s) 438
XhoI CTCGAG 1 cut(s) 37
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.