Rorug05G0547000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003464
Physical Location & Seq
Forward (+)
73628719 .. 73631668
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0547000.1

Sequence Viewer

Length: 1353 bp
ATGGAGAAAGCAAGTGCATTCCTCCGCCATCCCCCTCCTTGTAAGGGAAAACTCATCACTATCCTTAGCATTGATGGAGGTGGAGTCAAGGGGATTATCCCCGGAACTATTCTCGCCGTCTTAGAGAAGAAGCTTCAGGAAAAGGAGAGCAATACAAATGCAAGACTGGCCGATTATTTCGATGTGATTGCAGGGACGAGCACCGGTGGCCTTGTGACTGCCATGCTCACTGCTCCTGATGAAAACAATCGTCCCTTGTTCAGTGCTGACCAGATTGTCCCTTTTTATCTTGAAAACTGTCCTAAGATCTTTCCACCAAGCAAGTCTGCATTTCTAGGCAACATGGATGAATTGCCCTCTGGTTATAGTGATGACACGCTTCAACATTTGAAGCTAATGCCGAATTCAGGATTTTCTCGTAGTTGGTTAGAGTGGTTATGGAAATTAGTTGCTAAGCTTCCTGGTTCAATTGGAAAGCTACTGGGGAGTCCAAAATATGCTGCCACTGCGTTACGGGATATAGTTGAAGGAAAATTAGGAGACATACGGTTGAATCAGACATTGACCAATGTTGTAATCCCGAGTTATGATATAAGTCTGCTCCAACCAGCTATCTTCTCAACTTACGAGTTGAAAAGGAACGAGGAGCAAGATCCGGCTAAACTCTCTGATATATGCATGGCAACTTCAGCAGCACCAACCTATTTTCCTGCATACAAATTTGAAACCAATACTAGAGAATTCCATGTCGTTGACGGCGGAATGGCTGCTAATAATCCGGGTTTGGTTGCTATAACCGAAGTGACAAAAGAAATCAAGAATAAAAATCCTGACTTTCGAAAAGATTGGGCAGCTGATCAGGGCAATCGGTTCCTACTTATATCATTGGGTACTGGTTCACCAAAGCAAAGGAGTACATACAACGCCGACGAAGCAGCCAACTGGGGTATTGGGGGATGGATGATTCATTTGGATCCATTTTCTTGTCCATTGCAAGAAGTATTCACTGAAGCAAGTAGTGACATGGTCGATATCCACCTCACTGCCCTTTTCGAATCTATTTGGTCCAAGGGAACTTACCTTCGAATTCAGCATGATCAATTGCCTACAGACATGAATTCTATGGACAATGCTACAGAAGAAAACCTGAAAAATCTTGTGATAGCTGGGCAGGAGCTGTTACAGAAATCGGTTTCAAAGATGAATACAGCTACCGGTCGCCTTGAGACTATTAAACCTGAGATCACAAATGAAAAGGCTCTCGAAATGATGGCGGAAAAACTCGTTGAGGAGAGGAAGAAACGTCAAAGCCGAGACCATGACCATGGTCACCAGAAGGAAATTGATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

450

Amino Acids

49.86

Weight (kDa)

5.61

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 264 6.8e-23 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AciI CCGC 3 cut(s) 25, 759, 1274
AclWI GGATC 3 cut(s) 647, 968, 981
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 5 cut(s) 403, 719, 740, 1086, 1117
AcuI CTGAAG 3 cut(s) 119, 672, 1029
AfaI GTAC 2 cut(s) 892, 916
AfiI CCNNNNNNNGG 2 cut(s) 44, 407
AgeI ACCGGT 2 cut(s) 203, 1214
AgsI TTSAA 9 cut(s) 293, 383, 391, 468, 527, 553, 634, 725, 1197
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 9 cut(s) 133, 394, 457, 478, 611, 854, 1166, 1177, 1211
AluI AGCT 9 cut(s) 133, 394, 457, 478, 611, 854, 1166, 1177, 1211
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 3 cut(s) 534, 1220, 1308
AlwI GGATC 3 cut(s) 647, 968, 981
AlwNI CAGNNNCTG 1 cut(s) 1177
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 2 cut(s) 168, 208
ApeKI GCWGC 5 cut(s) 500, 692, 767, 851, 935
ApoI RAATTY 5 cut(s) 403, 719, 740, 1086, 1117
AsiGI ACCGGT 2 cut(s) 203, 1214
AspS9I GGNCC 1 cut(s) 1065
AsuC2I CCSGG 2 cut(s) 102, 780
AsuHPI GGTGA 2 cut(s) 891, 1322
AsuII TTCGAA 3 cut(s) 838, 1053, 1084
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 1 cut(s) 1065
BamHI GGATCC 1 cut(s) 973
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 5 cut(s) 487, 704, 754, 863, 947
BccI CCATC 4 cut(s) 36, 68, 951, 1264
BceAI ACGGC 2 cut(s) 101, 772
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 462
BclI TGATCA 2 cut(s) 856, 1096
BcnI CCSGG 2 cut(s) 102, 780
BcoDI GTCTC 3 cut(s) 534, 1220, 1308
BfaI CTAG 2 cut(s) 335, 735
BfmI CTRYAG 2 cut(s) 1107, 1134
BglII AGATCT 1 cut(s) 306
BisI GCNGC 5 cut(s) 501, 693, 768, 852, 936
BlpI GCTNAGC 1 cut(s) 453
BlsI GCNGC 5 cut(s) 502, 694, 769, 853, 937
Bme1390I CCNGG 3 cut(s) 102, 462, 780
Bme18I GGWCC 1 cut(s) 1065
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 1 cut(s) 1065
BmiI GGNNCC 2 cut(s) 872, 975
BmrFI CCNGG 3 cut(s) 102, 462, 780
BmrI ACTGGG 2 cut(s) 491, 952
BmsI GCATC 1 cut(s) 1336
BmuI ACTGGG 2 cut(s) 491, 952
BoxI GACNNNNGTC 1 cut(s) 1326
Bpu10I CCTNAGC 1 cut(s) 65
Bpu1102I GCTNAGC 1 cut(s) 453
Bpu14I TTCGAA 3 cut(s) 838, 1053, 1084
BpuEI CTTGAG 1 cut(s) 1244
BpuMI CCSGG 2 cut(s) 102, 780
BsaI GGTCTC 1 cut(s) 1308
BsaJI CCNNGG 3 cut(s) 100, 1068, 1324
BsaWI WCCGGW 2 cut(s) 203, 1214
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 407
Bse118I RCCGGY 2 cut(s) 203, 1214
Bse1I ACTGG 4 cut(s) 171, 486, 898, 947
Bse3DI GCAATG 1 cut(s) 989
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 3 cut(s) 100, 1068, 1324
BseGI GGATG 4 cut(s) 28, 352, 962, 966
BseLI CCNNNNNNNGG 2 cut(s) 44, 407
BseMI GCAATG 1 cut(s) 989
BseMII CTCAG 1 cut(s) 1230
BseNI ACTGG 4 cut(s) 171, 486, 898, 947
BseRI GAGGAG 2 cut(s) 659, 1304
BseXI GCAGC 5 cut(s) 487, 704, 754, 863, 947
BseYI CCCAGC 1 cut(s) 1166
Bsh1285I CGRYCG 1 cut(s) 1219
BshFI GGCC 2 cut(s) 170, 210
BshTI ACCGGT 2 cut(s) 203, 1214
BsiEI CGRYCG 1 cut(s) 1219
BsiHKAI GWGCWC 1 cut(s) 203
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 5 cut(s) 102, 204, 656, 779, 1215
BslFI GGGAC 3 cut(s) 208, 237, 263
BslI CCNNNNNNNGG 2 cut(s) 44, 407
BsmAI GTCTC 3 cut(s) 534, 1220, 1308
BsmFI GGGAC 3 cut(s) 208, 237, 263
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 170, 210
Bso31I GGTCTC 1 cut(s) 1308
BsoBI CYCGRG 1 cut(s) 580
Bsp119I TTCGAA 3 cut(s) 838, 1053, 1084
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 6 cut(s) 306, 652, 856, 973, 1096, 1242
Bsp1720I GCTNAGC 1 cut(s) 453
Bsp19I CCATGG 1 cut(s) 1324
BspACI CCGC 3 cut(s) 25, 759, 1274
BspANI GGCC 2 cut(s) 170, 210
BspCNI CTCAG 1 cut(s) 1231
BspLI GGNNCC 2 cut(s) 872, 975
BspPI GGATC 3 cut(s) 647, 968, 981
BspT104I TTCGAA 3 cut(s) 838, 1053, 1084
BspTNI GGTCTC 1 cut(s) 1308
BsrDI GCAATG 1 cut(s) 989
BsrFI RCCGGY 2 cut(s) 203, 1214
BsrI ACTGG 4 cut(s) 171, 486, 898, 947
BssAI RCCGGY 2 cut(s) 203, 1214
BssECI CCNNGG 3 cut(s) 100, 1068, 1324
BssMI GATC 6 cut(s) 306, 652, 856, 973, 1096, 1242
BssT1I CCWWGG 2 cut(s) 1068, 1324
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 2 cut(s) 299, 549
BstBI TTCGAA 3 cut(s) 838, 1053, 1084
BstDEI CTNAG 5 cut(s) 65, 121, 303, 453, 1239
BstDSI CCRYGG 1 cut(s) 1324
BstEII GGTNACC 1 cut(s) 1328
BstF5I GGATG 4 cut(s) 28, 352, 962, 966
BstKTI GATC 6 cut(s) 309, 655, 859, 976, 1099, 1245
BstMAI GTCTC 3 cut(s) 534, 1220, 1308
BstMBI GATC 6 cut(s) 306, 652, 856, 973, 1096, 1242
BstMCI CGRYCG 1 cut(s) 1219
BstMWI GCNNNNNNNGC 5 cut(s) 167, 207, 506, 689, 932
BstNI CCWGG 1 cut(s) 462
BstPAI GACNNNNGTC 1 cut(s) 1326
BstPI GGTNACC 1 cut(s) 1328
BstSCI CCNGG 3 cut(s) 100, 460, 778
BstSFI CTRYAG 2 cut(s) 1107, 1134
BstV1I GCAGC 5 cut(s) 487, 704, 754, 863, 947
BstX2I RGATCY 3 cut(s) 306, 652, 973
BstXI CCANNNNNNTGG 1 cut(s) 1325
BstYI RGATCY 3 cut(s) 306, 652, 973
BsuRI GGCC 2 cut(s) 170, 210
BtgI CCRYGG 1 cut(s) 1324
BtsCI GGATG 4 cut(s) 28, 352, 962, 966
BtsI GCAGTG 3 cut(s) 228, 504, 1041
BtsIMutI CAGTG 5 cut(s) 228, 268, 504, 1005, 1041
CaiI CAGNNNCTG 1 cut(s) 1177
Cfr10I RCCGGY 2 cut(s) 203, 1214
Cfr13I GGNCC 1 cut(s) 1065
Csp6I GTAC 2 cut(s) 891, 915
CspAI ACCGGT 2 cut(s) 203, 1214
CviAII CATG 9 cut(s) 223, 343, 679, 746, 1024, 1094, 1114, 1319, 1325
CviQI GTAC 2 cut(s) 891, 915
DdeI CTNAG 5 cut(s) 65, 121, 303, 453, 1239
DpnI GATC 6 cut(s) 308, 654, 858, 975, 1098, 1244
DpnII GATC 6 cut(s) 306, 652, 856, 973, 1096, 1242
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 168
EciI GGCGGA 3 cut(s) 14, 774, 1289
Eco130I CCWWGG 2 cut(s) 1068, 1324
Eco31I GGTCTC 1 cut(s) 1308
Eco32I GATATC 1 cut(s) 1033
Eco47I GGWCC 1 cut(s) 1065
Eco57I CTGAAG 3 cut(s) 119, 672, 1029
Eco88I CYCGRG 1 cut(s) 580
Eco91I GGTNACC 1 cut(s) 1328
EcoO65I GGTNACC 1 cut(s) 1328
EcoRI GAATTC 4 cut(s) 403, 740, 1086, 1117
EcoRII CCWGG 1 cut(s) 460
EcoRV GATATC 1 cut(s) 1033
EcoT14I CCWWGG 2 cut(s) 1068, 1324
EcoT22I ATGCAT 1 cut(s) 680
ErhI CCWWGG 2 cut(s) 1068, 1324
FaeI CATG 9 cut(s) 226, 346, 682, 749, 1027, 1097, 1117, 1322, 1328
FaqI GGGAC 3 cut(s) 208, 237, 263
FatI CATG 9 cut(s) 222, 342, 678, 745, 1023, 1093, 1113, 1318, 1324
FbaI TGATCA 2 cut(s) 856, 1096
Fnu4HI GCNGC 5 cut(s) 501, 693, 768, 852, 936
FokI GGATG 4 cut(s) 15, 359, 969, 973
Fsp4HI GCNGC 5 cut(s) 501, 693, 768, 852, 936
FspBI CTAG 2 cut(s) 335, 735
GluI GCNGC 5 cut(s) 501, 693, 768, 852, 936
GsaI CCCAGC 1 cut(s) 1170
HaeIII GGCC 2 cut(s) 170, 210
HapII CCGG 5 cut(s) 102, 204, 656, 779, 1215
Hin1II CATG 9 cut(s) 226, 346, 682, 749, 1027, 1097, 1117, 1322, 1328
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HindIII AAGCTT 2 cut(s) 131, 455
HinfI GANTC 5 cut(s) 84, 487, 553, 964, 1055
HpaII CCGG 5 cut(s) 102, 204, 656, 779, 1215
HphI GGTGA 2 cut(s) 891, 1322
Hpy166II GTNNAC 2 cut(s) 754, 899
Hpy188I TCNGA 2 cut(s) 558, 670
Hpy188III TCNNGA 8 cut(s) 137, 236, 290, 408, 580, 817, 830, 1262
Hpy8I GTNNAC 2 cut(s) 754, 899
Hpy99I CGWCG 1 cut(s) 932
HpyAV CCTTC 3 cut(s) 521, 1091, 1330
HpyCH4III ACNGT 2 cut(s) 299, 549
HpyCH4IV ACGT 1 cut(s) 1303
HpyCH4V TGCA 7 cut(s) 17, 161, 191, 329, 678, 713, 994
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 207, 506, 689, 932
HpyF3I CTNAG 5 cut(s) 65, 121, 303, 453, 1239
HpySE526I ACGT 1 cut(s) 1303
Hsp92II CATG 9 cut(s) 226, 346, 682, 749, 1027, 1097, 1117, 1322, 1328
Ksp22I TGATCA 2 cut(s) 856, 1096
Kzo9I GATC 6 cut(s) 306, 652, 856, 973, 1096, 1242
LmnI GCTCC 4 cut(s) 238, 606, 646, 1174
Lsp1109I GCAGC 5 cut(s) 487, 704, 754, 863, 947
LweI GCATC 1 cut(s) 1336
MaeI CTAG 2 cut(s) 335, 735
MaeII ACGT 1 cut(s) 1303
MaeIII GTNAC 6 cut(s) 214, 510, 802, 1019, 1179, 1328
MalI GATC 6 cut(s) 308, 654, 858, 975, 1098, 1244
MboI GATC 6 cut(s) 306, 652, 856, 973, 1096, 1242
MboII GAAGA 4 cut(s) 139, 607, 1151, 1309
MfeI CAATTG 2 cut(s) 468, 1100
MflI RGATCY 3 cut(s) 306, 652, 973
MhlI GDGCHC 1 cut(s) 203
MlyI GAGTC 2 cut(s) 93, 496
MmeI TCCRAC 1 cut(s) 628
MnlI CCTC 8 cut(s) 32, 45, 71, 367, 637, 1049, 1282, 1287
Mph1103I ATGCAT 1 cut(s) 680
MseI TTAA 1 cut(s) 1233
MslI CAYNNNNRTG 1 cut(s) 1323
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 5 cut(s) 102, 204, 656, 779, 1215
MspR9I CCNGG 3 cut(s) 102, 462, 780
MunI CAATTG 2 cut(s) 468, 1100
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 5 cut(s) 167, 207, 506, 689, 932
NciI CCSGG 2 cut(s) 102, 780
NcoI CCATGG 1 cut(s) 1324
NdeII GATC 6 cut(s) 306, 652, 856, 973, 1096, 1242
NlaIII CATG 9 cut(s) 226, 346, 682, 749, 1027, 1097, 1117, 1322, 1328
NlaIV GGNNCC 2 cut(s) 872, 975
NmeAIII GCCGAG 1 cut(s) 1337
NmuCI GTSAC 4 cut(s) 214, 802, 1019, 1328
NsiI ATGCAT 1 cut(s) 680
NspV TTCGAA 3 cut(s) 838, 1053, 1084
PcsI WCGNNNNNNNCGW 1 cut(s) 1309
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 553, 964, 1055
PflFI GACNNNGTC 1 cut(s) 1025
PinAI ACCGGT 2 cut(s) 203, 1214
PkrI GCNGC 5 cut(s) 502, 694, 769, 853, 937
PleI GAGTC 2 cut(s) 92, 495
PpsI GAGTC 2 cut(s) 92, 495
PshAI GACNNNNGTC 1 cut(s) 1326
Psp6I CCWGG 1 cut(s) 460
PspEI GGTNACC 1 cut(s) 1328
PspFI CCCAGC 1 cut(s) 1166
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 2 cut(s) 872, 975
PspPI GGNCC 1 cut(s) 1065
PstNI CAGNNNCTG 1 cut(s) 1177
PsuI RGATCY 3 cut(s) 306, 652, 973
PsyI GACNNNGTC 1 cut(s) 1025
PvuII CAGCTG 1 cut(s) 854
RsaI GTAC 2 cut(s) 892, 916
RsaNI GTAC 2 cut(s) 891, 915
RseI CAYNNNNRTG 1 cut(s) 1323
SaqAI TTAA 1 cut(s) 1233
SatI GCNGC 5 cut(s) 501, 693, 768, 852, 936
Sau3AI GATC 6 cut(s) 306, 652, 856, 973, 1096, 1242
Sau96I GGNCC 1 cut(s) 1065
SchI GAGTC 2 cut(s) 93, 496
ScrFI CCNGG 3 cut(s) 102, 462, 780
SduI GDGCHC 1 cut(s) 203
SfaNI GCATC 1 cut(s) 1336
SfcI CTRYAG 2 cut(s) 1107, 1134
SfuI TTCGAA 3 cut(s) 838, 1053, 1084
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 1 cut(s) 1065
SmiMI CAYNNNNRTG 1 cut(s) 1323
SmlI CTYRAG 1 cut(s) 1223
SmoI CTYRAG 1 cut(s) 1223
SsiI CCGC 3 cut(s) 25, 759, 1274
SspMI CTAG 2 cut(s) 335, 735
StyD4I CCNGG 3 cut(s) 100, 460, 778
StyI CCWWGG 2 cut(s) 1068, 1324
TaaI ACNGT 2 cut(s) 299, 549
TaiI ACGT 1 cut(s) 1306
TaqI TCGA 6 cut(s) 180, 838, 1029, 1053, 1084, 1263
TatI WGTACW 1 cut(s) 914
TfiI GAWTC 3 cut(s) 553, 964, 1055
Tru1I TTAA 1 cut(s) 1233
Tru9I TTAA 1 cut(s) 1233
TscAI CASTG 5 cut(s) 235, 268, 511, 1012, 1048
TseFI GTSAC 4 cut(s) 214, 802, 1019, 1328
TseI GCWGC 5 cut(s) 500, 692, 767, 851, 935
Tsp45I GTSAC 4 cut(s) 214, 802, 1019, 1328
TspDTI ATGAA 6 cut(s) 255, 363, 956, 1130, 1217, 1266
TspRI CASTG 5 cut(s) 235, 268, 511, 1012, 1048
Tth111I GACNNNGTC 1 cut(s) 1025
VpaK11BI GGWCC 1 cut(s) 1065
XapI RAATTY 5 cut(s) 403, 719, 740, 1086, 1117
XspI CTAG 2 cut(s) 335, 735
Zsp2I ATGCAT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.