Rh4BG024500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003464
Physical Location & Seq
Reverse (-)
3974341 .. 3989896
15556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG024500.1

Sequence Viewer

Length: 261 bp
ATGAGAATTGTTGAACTTTCTTTTAAGAATCTCGTAGCTTCATCCTCCATCGGTCCATCCCTCCAGTCTGCTCCATCCCTCTTCCGCTGCTTTGGTAGATCTGCGATTCTGCGGCAATCCCAACTAGTTAACGACGCCGTCGATCACCTCAAATCCGGCGGCTACCGCGGCCTCTTCTCTCAGATCAAGGTTCGAGTGCAAGCTATTCGGGGGCTTCCACTTTTCTGCAAGGATACCCTGAACTCATCTCCAGTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

86

Amino Acids

9.39

Weight (kDa)

10.52

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 168
AciI CCGC 5 cut(s) 85, 112, 159, 166, 168
AcyI GRCGYC 1 cut(s) 135
AgsI TTSAA 1 cut(s) 14
AhlI ACTAGT 1 cut(s) 124
AluBI AGCT 2 cut(s) 38, 203
AluI AGCT 2 cut(s) 38, 203
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 137
AvaII GGWCC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 74
BccI CCATC 3 cut(s) 56, 64, 82
BceAI ACGGC 1 cut(s) 122
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciVI GTATCC 1 cut(s) 226
BcuI ACTAGT 1 cut(s) 124
BfaI CTAG 1 cut(s) 125
BfuI GTATCC 1 cut(s) 226
BglII AGATCT 1 cut(s) 98
BisI GCNGC 4 cut(s) 88, 113, 160, 169
BlsI GCNGC 4 cut(s) 89, 114, 161, 170
Bme18I GGWCC 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 53
BpmI CTGGAG 2 cut(s) 47, 234
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 166
Bse1I ACTGG 2 cut(s) 64, 251
BseDI CCNNGG 1 cut(s) 166
BseGI GGATG 3 cut(s) 41, 56, 74
BseMII CTCAG 1 cut(s) 194
BseNI ACTGG 2 cut(s) 64, 251
BseXI GCAGC 1 cut(s) 74
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 1 cut(s) 171
BsiSI CCGG 1 cut(s) 156
BsnI GGCC 1 cut(s) 171
Bsp143I GATC 3 cut(s) 98, 142, 183
BspACI CCGC 5 cut(s) 85, 112, 159, 166, 168
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 1 cut(s) 193
BspFNI CGCG 1 cut(s) 168
BsrI ACTGG 2 cut(s) 64, 251
BssECI CCNNGG 1 cut(s) 166
BssMI GATC 3 cut(s) 98, 142, 183
BssNI GRCGYC 1 cut(s) 135
Bst6I CTCTTC 2 cut(s) 86, 179
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 180
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 3 cut(s) 41, 56, 74
BstFNI CGCG 1 cut(s) 168
BstKTI GATC 3 cut(s) 101, 145, 186
BstMBI GATC 3 cut(s) 98, 142, 183
BstMWI GCNNNNNNNGC 2 cut(s) 165, 168
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 74
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
BsuI GTATCC 1 cut(s) 226
BsuRI GGCC 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 3 cut(s) 41, 56, 74
Cac8I GCNNGC 1 cut(s) 201
Cfr13I GGNCC 1 cut(s) 53
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 1 cut(s) 143
CviJI RGCY 5 cut(s) 38, 162, 171, 203, 214
CviKI_1 RGCY 5 cut(s) 38, 162, 171, 203, 214
DdeI CTNAG 1 cut(s) 180
DpnI GATC 3 cut(s) 100, 144, 185
DpnII GATC 3 cut(s) 98, 142, 183
Eam1104I CTCTTC 2 cut(s) 86, 179
EarI CTCTTC 2 cut(s) 86, 179
Eco47I GGWCC 1 cut(s) 53
Fnu4HI GCNGC 4 cut(s) 88, 113, 160, 169
FokI GGATG 3 cut(s) 28, 43, 61
Fsp4HI GCNGC 4 cut(s) 88, 113, 160, 169
FspBI CTAG 1 cut(s) 125
GluI GCNGC 4 cut(s) 88, 113, 160, 169
GsuI CTGGAG 2 cut(s) 47, 234
HaeIII GGCC 1 cut(s) 171
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 143
Hin1I GRCGYC 1 cut(s) 135
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 2 cut(s) 28, 106
HpaI GTTAAC 1 cut(s) 130
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 183
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 2 cut(s) 137, 143
HpyCH4V TGCA 2 cut(s) 199, 228
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 168
HpyF3I CTNAG 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 135
KspAI GTTAAC 1 cut(s) 130
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 3 cut(s) 98, 142, 183
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 3 cut(s) 77, 169, 251
Lsp1109I GCAGC 1 cut(s) 74
MaeI CTAG 1 cut(s) 125
MalI GATC 3 cut(s) 100, 144, 185
MboI GATC 3 cut(s) 98, 142, 183
MboII GAAGA 2 cut(s) 73, 166
MflI RGATCY 1 cut(s) 98
MluCI AATT 1 cut(s) 6
MnlI CCTC 5 cut(s) 55, 71, 89, 158, 182
MseI TTAA 2 cut(s) 24, 129
MspA1I CMGCKG 2 cut(s) 87, 168
MspI CCGG 1 cut(s) 156
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 165, 168
NdeII GATC 3 cut(s) 98, 142, 183
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PfeI GAWTC 2 cut(s) 28, 106
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 4 cut(s) 89, 114, 161, 170
PspPI GGNCC 1 cut(s) 53
PsuI RGATCY 1 cut(s) 98
PsyI GACNNNGTC 1 cut(s) 137
SacII CCGCGG 1 cut(s) 169
SaqAI TTAA 2 cut(s) 24, 129
SatI GCNGC 4 cut(s) 88, 113, 160, 169
Sau3AI GATC 3 cut(s) 98, 142, 183
Sau96I GGNCC 1 cut(s) 53
SetI ASST 4 cut(s) 40, 150, 192, 205
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 1 cut(s) 53
SpeI ACTAGT 1 cut(s) 124
Sse9I AATT 1 cut(s) 6
SsiI CCGC 5 cut(s) 85, 112, 159, 166, 168
SspMI CTAG 1 cut(s) 125
TaqI TCGA 2 cut(s) 141, 193
TaqII GACCGA 1 cut(s) 41
TasI AATT 1 cut(s) 6
TauI GCSGC 3 cut(s) 115, 162, 171
TfiI GAWTC 2 cut(s) 28, 106
Tru1I TTAA 2 cut(s) 24, 129
Tru9I TTAA 2 cut(s) 24, 129
TseI GCWGC 1 cut(s) 87
TspDTI ATGAA 1 cut(s) 30
Tth111I GACNNNGTC 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 53
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.