Rh5DG256400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003464
Physical Location & Seq
Reverse (-)
30209646 .. 30211816
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG256400.1

Sequence Viewer

Length: 360 bp
ATGGGAGAGCAAAGGAGGCCGGAGCAATGGGAGGTAGGAGGAGGTCACTGCGTTTTGAGAGATGAGGCCGAAGCAATCTGCTTTGTGTTCTTGATCGGAGAGAGAAAAATGGGCAGCAAACAAAGGCAGGTGTGCAAGTTTGAGATGGAATTGAAGTCGATGATGCTGCAGAAGATGAATGGTTTTGCCGCAGCAGAGTCGTCATCATCATCAACTGGTAATAATCAGGCCAAAACGACGTTGCGTGTGTTTGGGGGTGGAGTGAATCACCAGCCACAGAGAACGACCGTTGATGATGAGAGAAGAAGAATAGAAAAGGCTGAAAATCTGGTGCACTTGATTTGCTGGGGACCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

13.43

Weight (kDa)

8.44

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 118
Acc36I ACCTGC 1 cut(s) 118
AciI CCGC 1 cut(s) 189
AgsI TTSAA 1 cut(s) 154
Alw21I GWGCWC 1 cut(s) 336
Alw44I GTGCAC 1 cut(s) 332
AoxI GGCC 3 cut(s) 17, 66, 228
ApaLI GTGCAC 1 cut(s) 332
ApeKI GCWGC 3 cut(s) 114, 166, 191
AspS9I GGNCC 1 cut(s) 350
AsuHPI GGTGA 1 cut(s) 260
AvaII GGWCC 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 3 cut(s) 126, 153, 203
BccI CCATC 1 cut(s) 139
BcgI CGANNNNNNTGC 4 cut(s) 148, 180, 182, 214
BfmI CTRYAG 1 cut(s) 167
BfuAI ACCTGC 1 cut(s) 118
BisI GCNGC 4 cut(s) 115, 167, 189, 192
BlsI GCNGC 4 cut(s) 116, 168, 190, 193
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 350
BmiI GGNNCC 2 cut(s) 351, 352
BmsI GCATC 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 220
Bse3DI GCAATG 1 cut(s) 32
BseMI GCAATG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 3 cut(s) 126, 153, 203
BseYI CCCAGC 1 cut(s) 345
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 3 cut(s) 19, 68, 230
BsiEI CGRYCG 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 336
BsiSI CCGG 1 cut(s) 20
BsnI GGCC 3 cut(s) 19, 68, 230
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 1 cut(s) 93
BspACI CCGC 1 cut(s) 189
BspANI GGCC 3 cut(s) 19, 68, 230
BspLI GGNNCC 2 cut(s) 351, 352
BspMAI CTGCAG 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 32
BsrI ACTGG 1 cut(s) 220
BssMI GATC 1 cut(s) 93
Bst4CI ACNGT 1 cut(s) 289
BstKTI GATC 1 cut(s) 96
BstMBI GATC 1 cut(s) 93
BstMCI CGRYCG 1 cut(s) 288
BstMWI GCNNNNNNNGC 1 cut(s) 16
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 3 cut(s) 126, 153, 203
BsuRI GGCC 3 cut(s) 19, 68, 230
BtsI GCAGTG 1 cut(s) 46
BtsIMutI CAGTG 1 cut(s) 46
BveI ACCTGC 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 350
CviJI RGCY 5 cut(s) 19, 68, 230, 274, 320
CviKI_1 RGCY 5 cut(s) 19, 68, 230, 274, 320
DpnI GATC 1 cut(s) 95
DpnII GATC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 350
Fnu4HI GCNGC 4 cut(s) 115, 167, 189, 192
Fsp4HI GCNGC 4 cut(s) 115, 167, 189, 192
GluI GCNGC 4 cut(s) 115, 167, 189, 192
GsaI CCCAGC 1 cut(s) 349
HaeIII GGCC 3 cut(s) 19, 68, 230
HapII CCGG 1 cut(s) 20
HinfI GANTC 2 cut(s) 197, 265
HpaII CCGG 1 cut(s) 20
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 1 cut(s) 98
Hpy188III TCNNGA 1 cut(s) 91
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 1 cut(s) 241
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 3 cut(s) 135, 169, 334
HpyF10VI GCNNNNNNNGC 1 cut(s) 16
HpySE526I ACGT 1 cut(s) 239
KflI GGGWCCC 1 cut(s) 350
Kzo9I GATC 1 cut(s) 93
LmnI GCTCC 1 cut(s) 22
LpnPI CCDG 7 cut(s) 33, 113, 201, 212, 284, 314, 331
Lsp1109I GCAGC 3 cut(s) 126, 153, 203
LweI GCATC 1 cut(s) 153
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 44
MalI GATC 1 cut(s) 95
MboI GATC 1 cut(s) 93
MboII GAAGA 3 cut(s) 184, 315, 318
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 2 cut(s) 149, 355
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 5 cut(s) 9, 25, 32, 35, 58
MseI TTAA 1 cut(s) 358
MspI CCGG 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 16
NdeII GATC 1 cut(s) 93
NlaIV GGNNCC 2 cut(s) 351, 352
NmuCI GTSAC 1 cut(s) 44
PaqCI CACCTGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 265
PkrI GCNGC 4 cut(s) 116, 168, 190, 193
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PpuMI RGGWCCY 1 cut(s) 350
Psp5II RGGWCCY 1 cut(s) 350
PspFI CCCAGC 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 351, 352
PspPI GGNCC 1 cut(s) 350
PspPPI RGGWCCY 1 cut(s) 350
PstI CTGCAG 1 cut(s) 171
SaqAI TTAA 1 cut(s) 358
SatI GCNGC 4 cut(s) 115, 167, 189, 192
Sau3AI GATC 1 cut(s) 93
Sau96I GGNCC 1 cut(s) 350
SchI GAGTC 1 cut(s) 206
SduI GDGCHC 1 cut(s) 336
SetI ASST 4 cut(s) 36, 46, 132, 242
SfaNI GCATC 1 cut(s) 153
SfcI CTRYAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 350
Sse9I AATT 2 cut(s) 149, 355
SsiI CCGC 1 cut(s) 189
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 158
TasI AATT 2 cut(s) 149, 355
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 1 cut(s) 358
Tru9I TTAA 1 cut(s) 358
TscAI CASTG 1 cut(s) 53
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 3 cut(s) 114, 166, 191
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 1 cut(s) 191
TspRI CASTG 1 cut(s) 53
VneI GTGCAC 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.