Rorug06G0086300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001416
Physical Location & Seq
Reverse (-)
11585973 .. 11590159
4187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0086300.1

Sequence Viewer

Length: 1281 bp
ATGGCGGACGATGAATGGACACCGCGAGAGGAAATTCCAGGAGAGGAGCTTCCTAATTACGAAGAGGTGGTCCTAGCTGTAAAGAATGTTGCTGTTGTAGTCCTTTATGGTATATATGTTGCTGGTGTGGTCCTTCAAGGTCACCCGGATTATTTTACCGTGAAGATGTACAATGGGGGATATTTTAAGGAGAACCAATATATAGGTGGGAAGGTGTCCTATTATGACTCTATTGATATGGACAAGATGTCACTTGTGGAACTTGATGGTATGGTTAGGGGGTTGAACCAAGACTATAGTGGAAAGAGGATAGATTATTGGTATAAGGTTGGGACTGAGGATGAAGCTTTAACAAAGCTTTCCACTGATCTTGATGCTATTACAATGTGCTTGTGTGTTCCACAAATTAGGCTTGTTATTATATACTTGGATCATTGGGAGCTTAAGAGGGACTATGAAGAGATAGATGATGATGAGGATGATGAGCTGTTTATGTATGAAGAAGATGAGTTCCATGATTTTGCTTCTAGTCAAGCTGGTTCATGTGGAGTTATTATCGAAGAATTGTCGGATTCACTGAGGAGGAAGCCAGGGGTAGTGATAAAGCAACTTCCAGATTCACCGAGGAAAGATGTCCCGAGTAGGACAGCAAAGAAGGCTTCTGGAATTGTGATAAGGGAGTTGGAATGGCATCCAACTGTGCCTACTCAAGCTAGCCAAGTGGGAGTGCTTGATCCTAAAGACAAAGACAACCTTGTGGATGTTAATGTGTCCACCTATGAAACAAGGAGCTCTAAATCTAGTGTACCCAATGAAGAGGATGGTGATGCTTATGATTCCAATGATTCTAAAGGTTTGCTATATGAGGACAGTTCAGAAGATGAAGACTATGATCCTGAGTTTGACAATGAAGACTATAATGAGTATGGTGTAGATGATGATTGGATGGGTGACATGGAAACAAAGTTTAATGATAGGGGTGAGTGGATTGGAGGAGTTGGTGAAGGGTCCAATGCTGCAAATATGGATAATGAGGGAGGTATGCAAGATGAGGACAATGAACCAATGTATGGAGCAATAGATTCTGATGAGGAATGCATTGGGAAAGAAGCTGACTCAAATGGAGAGGAAGAAGGGGATAGTTTCCCAGAGTTCAACCCTAAAACTGATATGGTCAACCCTCATTTTTGCAAAGGCATGAAGTTTGCAACTGCAAAGATTTTGAGAGCTGCCATTAGAGAGAGAGAGCAATTCAGAGAGGGTGGGAAGCTGTGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

426

Amino Acids

48.16

Weight (kDa)

4.18

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 50 - 132 1.1e-10 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1070
AccII CGCG 1 cut(s) 25
AciI CCGC 2 cut(s) 5, 23
AclWI GGATC 3 cut(s) 438, 728, 887
AcsI RAATTY 1 cut(s) 33
AfaI GTAC 2 cut(s) 170, 807
AfiI CCNNNNNNNGG 1 cut(s) 1070
AflII CTTAAG 1 cut(s) 443
AgsI TTSAA 3 cut(s) 137, 286, 1156
AhdI GACNNNNNGTC 1 cut(s) 247
AjnI CCWGG 2 cut(s) 37, 589
Alw21I GWGCWC 1 cut(s) 794
AlwI GGATC 3 cut(s) 438, 728, 887
Ama87I CYCGRG 1 cut(s) 637
ApeKI GCWGC 2 cut(s) 1016, 1229
ApoI RAATTY 1 cut(s) 33
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
AspS9I GGNCC 3 cut(s) 70, 130, 1008
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 6 cut(s) 134, 612, 836, 962, 992, 1013
AsuNHI GCTAGC 1 cut(s) 713
AvaI CYCGRG 1 cut(s) 637
AvaII GGWCC 3 cut(s) 70, 130, 1008
BanII GRGCYC 1 cut(s) 794
BbsI GAAGAC 2 cut(s) 891, 918
Bbv12I GWGCWC 1 cut(s) 794
BbvI GCAGC 2 cut(s) 1003, 1216
BccI CCATC 3 cut(s) 260, 815, 940
BciT130I CCWGG 2 cut(s) 39, 591
BcnI CCSGG 1 cut(s) 146
BfaI CTAG 4 cut(s) 74, 528, 714, 801
BfmI CTRYAG 1 cut(s) 295
BfrI CTTAAG 1 cut(s) 443
BisI GCNGC 2 cut(s) 1017, 1230
BlsI GCNGC 2 cut(s) 1018, 1231
Bme1390I CCNGG 3 cut(s) 39, 146, 591
Bme18I GGWCC 3 cut(s) 70, 130, 1008
BmeRI GACNNNNNGTC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 637
BmgT120I GGNCC 3 cut(s) 70, 130, 1008
BmiI GGNNCC 1 cut(s) 1009
BmrFI CCNGG 3 cut(s) 39, 146, 591
BmsI GCATC 3 cut(s) 364, 700, 817
BmtI GCTAGC 1 cut(s) 717
BpiI GAAGAC 2 cut(s) 891, 918
BpuEI CTTGAG 1 cut(s) 693
BpuMI CCSGG 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 590, 623
Bsc4I CCNNNNNNNGG 1 cut(s) 1070
BseBI CCWGG 2 cut(s) 39, 591
BseDI CCNNGG 2 cut(s) 590, 623
BseGI GGATG 6 cut(s) 346, 484, 691, 766, 826, 951
BseLI CCNNNNNNNGG 1 cut(s) 1070
BseMII CTCAG 3 cut(s) 327, 569, 888
BseRI GAGGAG 3 cut(s) 59, 595, 1008
BseXI GCAGC 2 cut(s) 1003, 1216
Bsh1236I CGCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 794
BsiHKCI CYCGRG 1 cut(s) 637
BsiSI CCGG 1 cut(s) 146
BslFI GGGAC 3 cut(s) 346, 464, 620
BslI CCNNNNNNNGG 1 cut(s) 1070
BsmFI GGGAC 3 cut(s) 346, 464, 620
BsmI GAATGC 1 cut(s) 1100
BsoBI CYCGRG 1 cut(s) 637
Bsp1286I GDGCHC 1 cut(s) 794
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 4 cut(s) 367, 430, 733, 892
BspACI CCGC 2 cut(s) 5, 23
BspCNI CTCAG 3 cut(s) 328, 570, 889
BspFNI CGCG 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 1009
BspOI GCTAGC 1 cut(s) 717
BspPI GGATC 3 cut(s) 438, 728, 887
BspTI CTTAAG 1 cut(s) 443
BsrGI TGTACA 1 cut(s) 168
BssECI CCNNGG 2 cut(s) 590, 623
BssMI GATC 4 cut(s) 367, 430, 733, 892
Bst2UI CCWGG 2 cut(s) 39, 591
Bst4CI ACNGT 3 cut(s) 160, 700, 872
Bst6I CTCTTC 3 cut(s) 57, 453, 810
BstAFI CTTAAG 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 715
BstDEI CTNAG 3 cut(s) 336, 578, 897
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 6 cut(s) 346, 484, 691, 766, 826, 951
BstFNI CGCG 1 cut(s) 25
BstKTI GATC 4 cut(s) 370, 433, 736, 895
BstMBI GATC 4 cut(s) 367, 430, 733, 892
BstMWI GCNNNNNNNGC 1 cut(s) 656
BstNI CCWGG 2 cut(s) 39, 591
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 3 cut(s) 37, 144, 589
BstSFI CTRYAG 1 cut(s) 295
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 1003, 1216
BstV2I GAAGAC 2 cut(s) 891, 918
BtsCI GGATG 6 cut(s) 346, 484, 691, 766, 826, 951
BtsIMutI CAGTG 2 cut(s) 363, 575
Cac8I GCNNGC 1 cut(s) 715
Cfr13I GGNCC 3 cut(s) 70, 130, 1008
Csp6I GTAC 2 cut(s) 169, 806
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 4 cut(s) 515, 543, 955, 1198
CviQI GTAC 2 cut(s) 169, 806
DdeI CTNAG 3 cut(s) 336, 578, 897
DpnI GATC 4 cut(s) 369, 432, 735, 894
DpnII GATC 4 cut(s) 367, 430, 733, 892
DriI GACNNNNNGTC 1 cut(s) 247
Eam1104I CTCTTC 3 cut(s) 57, 453, 810
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 3 cut(s) 57, 453, 810
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 792
Eco24I GRGCYC 1 cut(s) 794
Eco47I GGWCC 3 cut(s) 70, 130, 1008
Eco53kI GAGCTC 1 cut(s) 792
Eco88I CYCGRG 1 cut(s) 637
Eco91I GGTNACC 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 792
EcoO65I GGTNACC 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 37, 589
EcoT22I ATGCAT 1 cut(s) 1100
EcoT38I GRGCYC 1 cut(s) 794
FaeI CATG 4 cut(s) 518, 546, 958, 1201
FalI AAGNNNNNCTT 2 cut(s) 738, 770
FaqI GGGAC 3 cut(s) 346, 464, 620
FatI CATG 4 cut(s) 514, 542, 954, 1197
Fnu4HI GCNGC 2 cut(s) 1017, 1230
FokI GGATG 6 cut(s) 353, 491, 678, 773, 833, 958
FriOI GRGCYC 1 cut(s) 794
Fsp4HI GCNGC 2 cut(s) 1017, 1230
FspBI CTAG 4 cut(s) 74, 528, 714, 801
GluI GCNGC 2 cut(s) 1017, 1230
HapII CCGG 1 cut(s) 146
Hin1II CATG 4 cut(s) 518, 546, 958, 1201
HincII GTYRAC 1 cut(s) 1177
HindII GTYRAC 1 cut(s) 1177
HindIII AAGCTT 2 cut(s) 345, 356
HinfI GANTC 7 cut(s) 227, 572, 617, 836, 845, 1082, 1115
HpaII CCGG 1 cut(s) 146
HphI GGTGA 6 cut(s) 134, 612, 836, 962, 992, 1013
Hpy166II GTNNAC 3 cut(s) 774, 806, 1177
Hpy188I TCNGA 4 cut(s) 571, 877, 1087, 1256
Hpy188III TCNNGA 5 cut(s) 371, 614, 637, 663, 896
Hpy8I GTNNAC 3 cut(s) 774, 806, 1177
HpyAV CCTTC 5 cut(s) 143, 205, 649, 998, 1127
HpyCH4III ACNGT 3 cut(s) 160, 700, 872
HpyCH4V TGCA 6 cut(s) 1019, 1045, 1098, 1191, 1208, 1214
HpyF10VI GCNNNNNNNGC 1 cut(s) 656
HpyF3I CTNAG 3 cut(s) 336, 578, 897
Hsp92II CATG 4 cut(s) 518, 546, 958, 1201
Kzo9I GATC 4 cut(s) 367, 430, 733, 892
LmnI GCTCC 4 cut(s) 46, 439, 789, 1073
Lsp1109I GCAGC 2 cut(s) 1003, 1216
LweI GCATC 3 cut(s) 364, 700, 817
MaeI CTAG 4 cut(s) 74, 528, 714, 801
MaeIII GTNAC 3 cut(s) 140, 249, 950
MalI GATC 4 cut(s) 369, 432, 735, 894
MboI GATC 4 cut(s) 367, 430, 733, 892
MhlI GDGCHC 1 cut(s) 794
MluCI AATT 6 cut(s) 33, 55, 405, 563, 666, 1250
MlyI GAGTC 2 cut(s) 221, 1109
MmeI TCCRAC 3 cut(s) 549, 663, 719
Mph1103I ATGCAT 1 cut(s) 1100
MseI TTAA 5 cut(s) 186, 350, 444, 765, 969
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 3 cut(s) 39, 146, 591
Mva1269I GAATGC 1 cut(s) 1100
MvaI CCWGG 2 cut(s) 39, 591
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 1 cut(s) 656
NciI CCSGG 1 cut(s) 146
NdeII GATC 4 cut(s) 367, 430, 733, 892
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 4 cut(s) 518, 546, 958, 1201
NlaIV GGNNCC 1 cut(s) 1009
NmuCI GTSAC 3 cut(s) 140, 249, 950
NsiI ATGCAT 1 cut(s) 1100
PctI GAATGC 1 cut(s) 1100
PfeI GAWTC 5 cut(s) 572, 617, 836, 845, 1082
PflMI CCANNNNNTGG 1 cut(s) 1070
PfoI TCCNGGA 1 cut(s) 37
PkrI GCNGC 2 cut(s) 1018, 1231
PleI GAGTC 2 cut(s) 221, 1109
PpsI GAGTC 2 cut(s) 221, 1109
Psp124BI GAGCTC 1 cut(s) 794
Psp6I CCWGG 2 cut(s) 37, 589
PspEI GGTNACC 1 cut(s) 140
PspGI CCWGG 2 cut(s) 37, 589
PspN4I GGNNCC 1 cut(s) 1009
PspPI GGNCC 3 cut(s) 70, 130, 1008
RsaI GTAC 2 cut(s) 170, 807
RsaNI GTAC 2 cut(s) 169, 806
SacI GAGCTC 1 cut(s) 794
SaqAI TTAA 5 cut(s) 186, 350, 444, 765, 969
SatI GCNGC 2 cut(s) 1017, 1230
Sau3AI GATC 4 cut(s) 367, 430, 733, 892
Sau96I GGNCC 3 cut(s) 70, 130, 1008
SchI GAGTC 2 cut(s) 221, 1109
ScrFI CCNGG 3 cut(s) 39, 146, 591
SduI GDGCHC 1 cut(s) 794
SfaNI GCATC 3 cut(s) 364, 700, 817
SfcI CTRYAG 1 cut(s) 295
SinI GGWCC 3 cut(s) 70, 130, 1008
SmlI CTYRAG 2 cut(s) 443, 708
SmoI CTYRAG 2 cut(s) 443, 708
Sse9I AATT 6 cut(s) 33, 55, 405, 563, 666, 1250
SsiI CCGC 2 cut(s) 5, 23
SspMI CTAG 4 cut(s) 74, 528, 714, 801
SstI GAGCTC 1 cut(s) 794
StyD4I CCNGG 3 cut(s) 37, 144, 589
TaaI ACNGT 3 cut(s) 160, 700, 872
TaqI TCGA 1 cut(s) 558
TasI AATT 6 cut(s) 33, 55, 405, 563, 666, 1250
TatI WGTACW 1 cut(s) 168
TfiI GAWTC 5 cut(s) 572, 617, 836, 845, 1082
Tru1I TTAA 5 cut(s) 186, 350, 444, 765, 969
Tru9I TTAA 5 cut(s) 186, 350, 444, 765, 969
TscAI CASTG 2 cut(s) 370, 582
TseFI GTSAC 3 cut(s) 140, 249, 950
TseI GCWGC 2 cut(s) 1016, 1229
Tsp45I GTSAC 3 cut(s) 140, 249, 950
TspRI CASTG 2 cut(s) 370, 582
Van91I CCANNNNNTGG 1 cut(s) 1070
Vha464I CTTAAG 1 cut(s) 443
VpaK11BI GGWCC 3 cut(s) 70, 130, 1008
XapI RAATTY 1 cut(s) 33
XcmI CCANNNNNNNNNTGG 2 cut(s) 203, 296
XspI CTAG 4 cut(s) 74, 528, 714, 801
Zsp2I ATGCAT 1 cut(s) 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.