Rh6DG196300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001416
Physical Location & Seq
Forward (+)
35143925 .. 35145462
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG196300.1

Sequence Viewer

Length: 618 bp
ATGGAAGTTAGTAAGGAGAATACTGCGGATGGAACTAGGATGGTTGACCAAGAACTGATTGGTGGCTGCGGTCTACTGGAAGCAAGGAGCACATCAGTGAGTCCTTTGCACCATGTTGTAGTCTCATGCAAGGATCCTGAAAAGAAGAATATGCATATGCTAGTTGGTGACAATGCTAGCACAGATGGATTCGGAATGCAGGAAAGCATCTCAGGTGAGAAGGTTGATGAAAGTCCCGATACTGGCATATATGGTCCCAATACAGTGTCTTCAGAAAAAGTGGTTGGTTTGAAAAGTAATATCGCTGCTGGTATTGAGGTTTCAAATCTGAGATTAAATGAAGCATCAAGTTCCAAGGATTCTACTTCTGATGAACAGAGTAGATGCAATCTTTGTGATGATGTACTGGTTGATGGCAGTAGAGACAATAATATTGTTGCTGACAAAGGTAGTACTAGAAGTATGGAAGAAATAATTGGTATTAAGAATGCTGGTGATGGGGTTAAGTCGGTAGAAAAAAACGCCGCAAATATTGGATGCCGACAGTCTATTAGAAGAAAGAGACTCTTCATATGGCAAATCATTTTCAGATATTTCTCATACCAGGAAAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

22.34

Weight (kDa)

5.34

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 73
AciI CCGC 3 cut(s) 26, 69, 525
AclWI GGATC 2 cut(s) 128, 141
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 2 cut(s) 405, 454
AfiI CCNNNNNNNGG 1 cut(s) 242
AgsI TTSAA 2 cut(s) 292, 324
AjnI CCWGG 1 cut(s) 603
AjuI GAANNNNNNNTTGG 4 cut(s) 267, 299, 459, 491
AleI CACNNNNGTG 1 cut(s) 95
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 3 cut(s) 127, 417, 556
AlwI GGATC 2 cut(s) 128, 141
ApeKI GCWGC 2 cut(s) 66, 305
AspS9I GGNCC 1 cut(s) 254
AsuHPI GGTGA 3 cut(s) 179, 227, 506
AsuNHI GCTAGC 1 cut(s) 176
AvaII GGWCC 1 cut(s) 254
BamHI GGATCC 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 53, 292
BccI CCATC 5 cut(s) 23, 34, 179, 407, 491
BciT130I CCWGG 1 cut(s) 605
BcoDI GTCTC 3 cut(s) 127, 417, 556
BfaI CTAG 4 cut(s) 36, 161, 177, 456
BisI GCNGC 3 cut(s) 67, 306, 525
BlsI GCNGC 3 cut(s) 68, 307, 526
BmcAI AGTACT 1 cut(s) 454
Bme1390I CCNGG 1 cut(s) 605
Bme18I GGWCC 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 135, 256
BmrFI CCNGG 1 cut(s) 605
BmsI GCATC 4 cut(s) 216, 353, 374, 527
BmtI GCTAGC 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse1I ACTGG 3 cut(s) 81, 247, 411
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 3 cut(s) 34, 45, 542
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMII CTCAG 2 cut(s) 225, 320
BseNI ACTGG 3 cut(s) 81, 247, 411
BseXI GCAGC 2 cut(s) 53, 292
BsiHKAI GWGCWC 1 cut(s) 92
BslFI GGGAC 2 cut(s) 219, 240
BslI CCNNNNNNNGG 1 cut(s) 242
BsmAI GTCTC 3 cut(s) 127, 417, 556
BsmFI GGGAC 2 cut(s) 219, 240
BsmI GAATGC 2 cut(s) 201, 493
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 1 cut(s) 133
BspACI CCGC 3 cut(s) 26, 69, 525
BspCNI CTCAG 2 cut(s) 224, 321
BspLI GGNNCC 2 cut(s) 135, 256
BspOI GCTAGC 1 cut(s) 180
BspPI GGATC 2 cut(s) 128, 141
BsrI ACTGG 3 cut(s) 81, 247, 411
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 2 cut(s) 265, 546
Bst6I CTCTTC 1 cut(s) 572
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 2 cut(s) 211, 329
BstF5I GGATG 3 cut(s) 34, 45, 542
BstKTI GATC 1 cut(s) 136
BstMAI GTCTC 3 cut(s) 127, 417, 556
BstMBI GATC 1 cut(s) 133
BstNI CCWGG 1 cut(s) 605
BstSCI CCNGG 1 cut(s) 603
BstV1I GCAGC 2 cut(s) 53, 292
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BtsCI GGATG 3 cut(s) 34, 45, 542
BtsIMutI CAGTG 2 cut(s) 102, 270
Cac8I GCNNGC 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 254
Csp6I GTAC 2 cut(s) 404, 453
CviAII CATG 2 cut(s) 113, 126
CviJI RGCY 1 cut(s) 66
CviKI_1 RGCY 1 cut(s) 66
CviQI GTAC 2 cut(s) 404, 453
DdeI CTNAG 2 cut(s) 211, 329
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
Eco130I CCWWGG 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 254
Eco57I CTGAAG 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 2 cut(s) 116, 129
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 2 cut(s) 219, 240
FatI CATG 2 cut(s) 112, 125
FauNDI CATATG 2 cut(s) 156, 572
FblI GTMKAC 1 cut(s) 73
Fnu4HI GCNGC 3 cut(s) 67, 306, 525
FokI GGATG 3 cut(s) 41, 52, 549
Fsp4HI GCNGC 3 cut(s) 67, 306, 525
FspBI CTAG 4 cut(s) 36, 161, 177, 456
GluI GCNGC 3 cut(s) 67, 306, 525
Hin1II CATG 2 cut(s) 116, 129
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 4 cut(s) 100, 189, 359, 564
HphI GGTGA 3 cut(s) 179, 227, 506
Hpy166II GTNNAC 2 cut(s) 46, 74
Hpy188I TCNGA 5 cut(s) 194, 274, 330, 370, 590
Hpy188III TCNNGA 2 cut(s) 137, 236
Hpy8I GTNNAC 2 cut(s) 46, 74
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 2 cut(s) 265, 546
HpyCH4V TGCA 5 cut(s) 109, 129, 154, 199, 387
HpyF3I CTNAG 2 cut(s) 211, 329
Hsp92II CATG 2 cut(s) 116, 129
Kzo9I GATC 1 cut(s) 133
LmnI GCTCC 1 cut(s) 87
LpnPI CCDG 9 cut(s) 62, 150, 185, 198, 228, 294, 392, 477, 590
Lsp1109I GCAGC 2 cut(s) 53, 292
LweI GCATC 4 cut(s) 216, 353, 374, 527
MaeI CTAG 4 cut(s) 36, 161, 177, 456
MaeIII GTNAC 1 cut(s) 167
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 5 cut(s) 157, 261, 479, 559, 567
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 1 cut(s) 474
MlyI GAGTC 2 cut(s) 109, 558
MnlI CCTC 1 cut(s) 310
Mph1103I ATGCAT 1 cut(s) 156
MseI TTAA 3 cut(s) 335, 483, 504
MslI CAYNNNNRTG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 605
Mva1269I GAATGC 2 cut(s) 201, 493
MvaI CCWGG 1 cut(s) 605
NdeI CATATG 2 cut(s) 156, 572
NdeII GATC 1 cut(s) 133
NheI GCTAGC 1 cut(s) 176
NlaIII CATG 2 cut(s) 116, 129
NlaIV GGNNCC 2 cut(s) 135, 256
NmuCI GTSAC 1 cut(s) 167
NsiI ATGCAT 1 cut(s) 156
OliI CACNNNNGTG 1 cut(s) 95
PctI GAATGC 2 cut(s) 201, 493
PfeI GAWTC 2 cut(s) 189, 359
PkrI GCNGC 3 cut(s) 68, 307, 526
PleI GAGTC 2 cut(s) 108, 558
PpsI GAGTC 2 cut(s) 108, 558
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 2 cut(s) 135, 256
PspPI GGNCC 1 cut(s) 254
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 2 cut(s) 405, 454
RsaNI GTAC 2 cut(s) 404, 453
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 3 cut(s) 335, 483, 504
SatI GCNGC 3 cut(s) 67, 306, 525
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 1 cut(s) 254
ScaI AGTACT 1 cut(s) 454
SchI GAGTC 2 cut(s) 109, 558
ScrFI CCNGG 1 cut(s) 605
SduI GDGCHC 1 cut(s) 92
SetI ASST 4 cut(s) 217, 225, 321, 451
SfaNI GCATC 4 cut(s) 216, 353, 374, 527
SinI GGWCC 1 cut(s) 254
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 1 cut(s) 474
SsiI CCGC 3 cut(s) 26, 69, 525
SspI AATATT 2 cut(s) 433, 532
SspMI CTAG 4 cut(s) 36, 161, 177, 456
StyD4I CCNGG 1 cut(s) 603
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 265, 546
TasI AATT 1 cut(s) 474
TatI WGTACW 2 cut(s) 403, 452
TauI GCSGC 1 cut(s) 527
TfiI GAWTC 2 cut(s) 189, 359
Tru1I TTAA 3 cut(s) 335, 483, 504
Tru9I TTAA 3 cut(s) 335, 483, 504
TscAI CASTG 2 cut(s) 102, 270
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 2 cut(s) 66, 305
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 4 cut(s) 243, 354, 387, 559
TspRI CASTG 2 cut(s) 102, 270
VpaK11BI GGWCC 1 cut(s) 254
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XmiI GTMKAC 1 cut(s) 73
XspI CTAG 4 cut(s) 36, 161, 177, 456
ZrmI AGTACT 1 cut(s) 454
Zsp2I ATGCAT 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.