Rh1AG148900

U3 small nucleolar RNA-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003161
Physical Location & Seq
Forward (+)
28025180 .. 28086039
60860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG148900.1

Sequence Viewer

Length: 1158 bp
ATGGCAGACCACCGTGATTGGAGATCTTTGGCCTTCATGGTTCAACGTCGGTGTTCACAGGACCACCGCCTGGTGCAGCCACTGGTGCCGGTCGAGGATCGTTGGCGGCGTGGCTCGTCATTGGCGGTCGTCCGTCTCAGAGGGCACGCAAGGGAGACAACTGCCAAGTCCGGGTCGTGGACCAGTCCTGCGGGTTTGGGCCTCTCTTGTGGCTATGGATCTGTCAGGCTGGTTTGGGCCACCCATGTGGGTTTGGGCCTTTGCTTGTGGTTCTGTCATAGGCTGGATTTCACGCCAAGCAGTCGATACATGATGGTCGGTGGACACAAGGGCCATCTAGCTATTGTAGACATGAAGCAAATGAGCCTGATCAAAGAATTTCAGGAACATGGTGCAGCTTTAAGGCTTCGTTATCTTGAACACCAACTATTGGCATCAATAAATGAATATGGTCAGCTTCATTATCAGGATGTAACTGATGGTCGGATGATAGGTAGTTACAGGACAGGCTTTGGTCGTACTGATGTGATGCAGGTAAACCGCCCCAATGCACTTCTTGCTTTGGGTCATTCAGGTGGTAAAGTTACCATGTGGTGTCCTACAAGCTCTTCTGCTGTTATTGAGATGCTGTGCCACAAGGGGCCCATCTCGGCCATAGCTTTCCACCCTAGTAATCGTGATCTCATGGCCACATCTGGGAAAGAGAAAAAAGTTAAGCTTTGGGACGTAAGGAATCTTAAGTGGCCACTCCAAATGTTACCAGGTCAGGCAGAGACTGGATTTCAGTCAAAAGGGTCTGCTCGCTCGTGGAACTGGGTCTTTTCTACAGGGTCTGAGAGATTCTTCTGGGACTCAGAAATTGGCACAGTGAAACCTCCAAGAAAAGAGAAGCCAACAAAGGATGAGAAAGAGGCTGAGCTGGAAGCCGCTGTTGAAGTTGCCAAGGACATTGTCCCAAAGAATAAAACAAAGGGGAGGAGTAAGCCAAGTAAGATGAAGAAGAAGAAGCACGAGATAATTGCCAATGCGAAGAGACCTTTCTTGGAACAACAAATGAAAGGGGACGAACAAATGGCTAGAAAGAAGCAGAAAGGAATGGAGCAAGTGGAACTACCAACATCTTTGCAGCGGTTTTCTCGCAAGAAAGCGGCTACATGA

Protein Analysis

385

Amino Acids

43.57

Weight (kDa)

10.01

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 95 - 245 2e-15 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 96 - 247 8.5e-07 WDR5 beta-propeller domain
WD40_Prp19 PF24814 144 - 247 8.1e-08 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 161 - 245 1.2e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 186 - 247 1.5e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 523
AccB1I GGYRCC 1 cut(s) 85
AccB7I CCANNNNNTGG 2 cut(s) 70, 430
AccI GTMKAC 1 cut(s) 348
AciI CCGC 8 cut(s) 67, 106, 125, 191, 541, 927, 1129, 1148
AclWI GGATC 2 cut(s) 105, 226
AcoI YGGCCR 3 cut(s) 651, 687, 743
AcsI RAATTY 1 cut(s) 377
AfaI GTAC 1 cut(s) 520
AfiI CCNNNNNNNGG 5 cut(s) 70, 171, 177, 430, 696
AflII CTTAAG 1 cut(s) 737
AgsI TTSAA 3 cut(s) 44, 419, 935
AjnI CCWGG 2 cut(s) 69, 760
AleI CACNNNNGTG 1 cut(s) 245
AluBI AGCT 7 cut(s) 341, 398, 457, 606, 659, 718, 919
AluI AGCT 7 cut(s) 341, 398, 457, 606, 659, 718, 919
Alw26I GTCTC 4 cut(s) 140, 149, 767, 1027
AlwI GGATC 2 cut(s) 105, 226
AlwNI CAGNNNCTG 3 cut(s) 82, 776, 833
AoxI GGCC 9 cut(s) 30, 199, 237, 256, 331, 641, 651, 687, 743
ApaI GGGCCC 1 cut(s) 645
ApeKI GCWGC 3 cut(s) 76, 395, 1126
ApoI RAATTY 1 cut(s) 377
AspS9I GGNCC 8 cut(s) 61, 180, 199, 237, 256, 331, 641, 642
AsuC2I CCSGG 1 cut(s) 172
AvaII GGWCC 2 cut(s) 61, 180
BaeGI GKGCMC 2 cut(s) 147, 645
BalI TGGCCA 2 cut(s) 689, 745
BanI GGYRCC 1 cut(s) 85
BanII GRGCYC 1 cut(s) 645
BauI CACGAG 2 cut(s) 805, 1010
BbvI GCAGC 3 cut(s) 88, 407, 1138
BccI CCATC 4 cut(s) 307, 342, 473, 653
BcgI CGANNNNNNTGC 2 cut(s) 1001, 1035
BciT130I CCWGG 2 cut(s) 71, 762
BclI TGATCA 1 cut(s) 369
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 4 cut(s) 140, 149, 767, 1027
BfaI CTAG 3 cut(s) 338, 669, 1077
BfmI CTRYAG 1 cut(s) 825
BfrI CTTAAG 1 cut(s) 737
BfuAI ACCTGC 1 cut(s) 523
BglII AGATCT 1 cut(s) 23
BisI GCNGC 6 cut(s) 77, 107, 396, 927, 1127, 1149
BlpI GCTNAGC 1 cut(s) 915
BlsI GCNGC 6 cut(s) 78, 108, 397, 928, 1128, 1150
Bme1390I CCNGG 3 cut(s) 71, 172, 762
Bme18I GGWCC 2 cut(s) 61, 180
BmgT120I GGNCC 8 cut(s) 61, 180, 199, 237, 256, 331, 641, 642
BmiI GGNNCC 3 cut(s) 87, 642, 643
BmrFI CCNGG 3 cut(s) 71, 172, 762
BmrI ACTGGG 1 cut(s) 823
BmsI GCATC 3 cut(s) 443, 519, 615
BmuI ACTGGG 1 cut(s) 823
Bpu1102I GCTNAGC 1 cut(s) 915
BpuMI CCSGG 1 cut(s) 172
BsaI GGTCTC 1 cut(s) 1027
BsaJI CCNNGG 1 cut(s) 942
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 171, 177, 430, 696
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 4 cut(s) 87, 183, 781, 818
BseBI CCWGG 2 cut(s) 71, 762
BseDI CCNNGG 1 cut(s) 942
BseGI GGATG 3 cut(s) 475, 492, 907
BseLI CCNNNNNNNGG 5 cut(s) 70, 171, 177, 430, 696
BseMII CTCAG 4 cut(s) 151, 825, 867, 906
BseNI ACTGG 4 cut(s) 87, 183, 781, 818
BseRI GAGGAG 1 cut(s) 991
BseSI GKGCMC 2 cut(s) 147, 645
BseXI GCAGC 3 cut(s) 88, 407, 1138
BsgI GTGCAG 2 cut(s) 95, 414
Bsh1285I CGRYCG 2 cut(s) 93, 129
BshFI GGCC 9 cut(s) 32, 201, 239, 258, 333, 643, 653, 689, 745
BshNI GGYRCC 1 cut(s) 85
BsiEI CGRYCG 2 cut(s) 93, 129
BsiSI CCGG 2 cut(s) 89, 171
BslFI GGGAC 4 cut(s) 737, 863, 938, 1076
BslI CCNNNNNNNGG 5 cut(s) 70, 171, 177, 430, 696
BsmAI GTCTC 4 cut(s) 140, 149, 767, 1027
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 4 cut(s) 737, 863, 938, 1076
BsnI GGCC 9 cut(s) 32, 201, 239, 258, 333, 643, 653, 689, 745
Bso31I GGTCTC 1 cut(s) 1027
Bsp120I GGGCCC 1 cut(s) 641
Bsp1286I GDGCHC 2 cut(s) 147, 645
Bsp143I GATC 5 cut(s) 23, 97, 218, 369, 679
Bsp1720I GCTNAGC 1 cut(s) 915
BspACI CCGC 8 cut(s) 67, 106, 125, 191, 541, 927, 1129, 1148
BspANI GGCC 9 cut(s) 32, 201, 239, 258, 333, 643, 653, 689, 745
BspCNI CTCAG 4 cut(s) 150, 826, 866, 907
BspLI GGNNCC 3 cut(s) 87, 642, 643
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 2 cut(s) 105, 226
BspQI GCTCTTC 1 cut(s) 613
BspT107I GGYRCC 1 cut(s) 85
BspTI CTTAAG 1 cut(s) 737
BspTNI GGTCTC 1 cut(s) 1027
BsrFI RCCGGY 1 cut(s) 88
BsrI ACTGG 4 cut(s) 87, 183, 781, 818
BssAI RCCGGY 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 942
BssMI GATC 5 cut(s) 23, 97, 218, 369, 679
BssSI CACGAG 2 cut(s) 805, 1010
BssT1I CCWWGG 1 cut(s) 942
Bst2BI CACGAG 2 cut(s) 805, 1010
Bst2UI CCWGG 2 cut(s) 71, 762
Bst4CI ACNGT 2 cut(s) 14, 868
Bst6I CTCTTC 2 cut(s) 613, 1025
BstAFI CTTAAG 1 cut(s) 737
BstAPI GCANNNNNTGC 1 cut(s) 557
BstC8I GCNNGC 2 cut(s) 147, 802
BstDEI CTNAG 4 cut(s) 137, 834, 853, 915
BstF5I GGATG 3 cut(s) 475, 492, 907
BstKTI GATC 5 cut(s) 26, 100, 221, 372, 682
BstMAI GTCTC 4 cut(s) 140, 149, 767, 1027
BstMBI GATC 5 cut(s) 23, 97, 218, 369, 679
BstMCI CGRYCG 2 cut(s) 93, 129
BstMWI GCNNNNNNNGC 2 cut(s) 85, 557
BstNI CCWGG 2 cut(s) 71, 762
BstSCI CCNGG 3 cut(s) 69, 170, 760
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 2 cut(s) 147, 645
BstV1I GCAGC 3 cut(s) 88, 407, 1138
BstX2I RGATCY 2 cut(s) 23, 218
BstXI CCANNNNNNTGG 1 cut(s) 247
BstYI RGATCY 2 cut(s) 23, 218
BsuRI GGCC 9 cut(s) 32, 201, 239, 258, 333, 643, 653, 689, 745
BtsCI GGATG 3 cut(s) 475, 492, 907
BtsIMutI CAGTG 2 cut(s) 80, 873
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 2 cut(s) 147, 802
CaiI CAGNNNCTG 3 cut(s) 82, 776, 833
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 8 cut(s) 61, 180, 199, 237, 256, 331, 641, 642
CsiI ACCWGGT 1 cut(s) 760
Csp6I GTAC 1 cut(s) 519
CspCI CAANNNNNGTGG 1 cut(s) 34
CviAII CATG 8 cut(s) 37, 245, 310, 352, 389, 589, 685, 1155
CviQI GTAC 1 cut(s) 519
DdeI CTNAG 4 cut(s) 137, 834, 853, 915
DpnI GATC 5 cut(s) 25, 99, 220, 371, 681
DpnII GATC 5 cut(s) 23, 97, 218, 369, 679
EaeI YGGCCR 3 cut(s) 651, 687, 743
Eam1104I CTCTTC 2 cut(s) 613, 1025
EarI CTCTTC 2 cut(s) 613, 1025
Eco130I CCWWGG 1 cut(s) 942
Eco24I GRGCYC 1 cut(s) 645
Eco31I GGTCTC 1 cut(s) 1027
Eco47I GGWCC 2 cut(s) 61, 180
EcoO109I RGGNCCY 1 cut(s) 641
EcoRII CCWGG 2 cut(s) 69, 760
EcoT14I CCWWGG 1 cut(s) 942
EcoT38I GRGCYC 1 cut(s) 645
ErhI CCWWGG 1 cut(s) 942
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 8 cut(s) 40, 248, 313, 355, 392, 592, 688, 1158
FalI AAGNNNNNCTT 2 cut(s) 702, 734
FaqI GGGAC 4 cut(s) 737, 863, 938, 1076
FatI CATG 8 cut(s) 36, 244, 309, 351, 388, 588, 684, 1154
FauI CCCGC 1 cut(s) 184
FbaI TGATCA 1 cut(s) 369
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 6 cut(s) 77, 107, 396, 927, 1127, 1149
FokI GGATG 3 cut(s) 482, 499, 914
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 6 cut(s) 77, 107, 396, 927, 1127, 1149
FspBI CTAG 3 cut(s) 338, 669, 1077
GluI GCNGC 6 cut(s) 77, 107, 396, 927, 1127, 1149
HaeIII GGCC 9 cut(s) 32, 201, 239, 258, 333, 643, 653, 689, 745
HapII CCGG 2 cut(s) 89, 171
Hin1II CATG 8 cut(s) 40, 248, 313, 355, 392, 592, 688, 1158
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 3 cut(s) 733, 840, 851
HpaII CCGG 2 cut(s) 89, 171
Hpy166II GTNNAC 5 cut(s) 56, 180, 323, 349, 538
Hpy188I TCNGA 4 cut(s) 140, 486, 835, 856
Hpy188III TCNNGA 4 cut(s) 383, 416, 467, 677
Hpy8I GTNNAC 5 cut(s) 56, 180, 323, 349, 538
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 2 cut(s) 14, 868
HpyCH4IV ACGT 2 cut(s) 46, 726
HpyCH4V TGCA 5 cut(s) 76, 395, 532, 551, 1126
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 557
HpyF3I CTNAG 4 cut(s) 137, 834, 853, 915
HpySE526I ACGT 2 cut(s) 46, 726
Hsp92II CATG 8 cut(s) 40, 248, 313, 355, 392, 592, 688, 1158
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 5 cut(s) 23, 97, 218, 369, 679
LguI GCTCTTC 1 cut(s) 613
LmnI GCTCC 1 cut(s) 1099
Lsp1109I GCAGC 3 cut(s) 88, 407, 1138
LweI GCATC 3 cut(s) 443, 519, 615
MabI ACCWGGT 1 cut(s) 760
MaeI CTAG 3 cut(s) 338, 669, 1077
MaeII ACGT 2 cut(s) 46, 726
MaeIII GTNAC 4 cut(s) 472, 497, 583, 756
MalI GATC 5 cut(s) 25, 99, 220, 371, 681
MboI GATC 5 cut(s) 23, 97, 218, 369, 679
MboII GAAGA 6 cut(s) 600, 835, 1009, 1012, 1015, 1042
MflI RGATCY 2 cut(s) 23, 218
MhlI GDGCHC 2 cut(s) 147, 645
MlsI TGGCCA 2 cut(s) 689, 745
MluCI AATT 3 cut(s) 377, 858, 1017
MluNI TGGCCA 2 cut(s) 689, 745
MlyI GAGTC 1 cut(s) 845
MmeI TCCRAC 1 cut(s) 464
MnlI CCTC 6 cut(s) 88, 134, 212, 885, 904, 969
Mox20I TGGCCA 2 cut(s) 689, 745
MscI TGGCCA 2 cut(s) 689, 745
MseI TTAA 3 cut(s) 401, 714, 738
MslI CAYNNNNRTG 2 cut(s) 245, 573
Msp20I TGGCCA 2 cut(s) 689, 745
MspA1I CMGCKG 2 cut(s) 929, 1129
MspCI CTTAAG 1 cut(s) 737
MspI CCGG 2 cut(s) 89, 171
MspR9I CCNGG 3 cut(s) 71, 172, 762
MvaI CCWGG 2 cut(s) 71, 762
MwoI GCNNNNNNNGC 2 cut(s) 85, 557
NciI CCSGG 1 cut(s) 172
NdeII GATC 5 cut(s) 23, 97, 218, 369, 679
NlaIII CATG 8 cut(s) 40, 248, 313, 355, 392, 592, 688, 1158
NlaIV GGNNCC 3 cut(s) 87, 642, 643
NmeAIII GCCGAG 1 cut(s) 629
OliI CACNNNNGTG 1 cut(s) 245
PciSI GCTCTTC 1 cut(s) 613
PcsI WCGNNNNNNNCGW 1 cut(s) 106
PfeI GAWTC 2 cut(s) 733, 840
PflFI GACNNNGTC 1 cut(s) 950
PflMI CCANNNNNTGG 2 cut(s) 70, 430
PkrI GCNGC 6 cut(s) 78, 108, 397, 928, 1128, 1150
PleI GAGTC 1 cut(s) 845
PpsI GAGTC 1 cut(s) 845
Psp6I CCWGG 2 cut(s) 69, 760
PspGI CCWGG 2 cut(s) 69, 760
PspN4I GGNNCC 3 cut(s) 87, 642, 643
PspOMI GGGCCC 1 cut(s) 641
PspPI GGNCC 8 cut(s) 61, 180, 199, 237, 256, 331, 641, 642
PstNI CAGNNNCTG 3 cut(s) 82, 776, 833
PsuI RGATCY 2 cut(s) 23, 218
PsyI GACNNNGTC 1 cut(s) 950
RsaI GTAC 1 cut(s) 520
RsaNI GTAC 1 cut(s) 519
RseI CAYNNNNRTG 2 cut(s) 245, 573
SapI GCTCTTC 1 cut(s) 613
SaqAI TTAA 3 cut(s) 401, 714, 738
SatI GCNGC 6 cut(s) 77, 107, 396, 927, 1127, 1149
Sau3AI GATC 5 cut(s) 23, 97, 218, 369, 679
Sau96I GGNCC 8 cut(s) 61, 180, 199, 237, 256, 331, 641, 642
SchI GAGTC 1 cut(s) 845
ScrFI CCNGG 3 cut(s) 71, 172, 762
SduI GDGCHC 2 cut(s) 147, 645
SexAI ACCWGGT 1 cut(s) 760
SfaNI GCATC 3 cut(s) 443, 519, 615
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 2 cut(s) 61, 180
SmiMI CAYNNNNRTG 2 cut(s) 245, 573
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
Sse9I AATT 3 cut(s) 377, 858, 1017
SsiI CCGC 8 cut(s) 67, 106, 125, 191, 541, 927, 1129, 1148
SspMI CTAG 3 cut(s) 338, 669, 1077
StyD4I CCNGG 3 cut(s) 69, 170, 760
StyI CCWWGG 1 cut(s) 942
TaaI ACNGT 2 cut(s) 14, 868
TaiI ACGT 2 cut(s) 49, 729
TaqI TCGA 2 cut(s) 93, 304
TasI AATT 3 cut(s) 377, 858, 1017
TauI GCSGC 3 cut(s) 109, 929, 1151
TfiI GAWTC 2 cut(s) 733, 840
Tru1I TTAA 3 cut(s) 401, 714, 738
Tru9I TTAA 3 cut(s) 401, 714, 738
TscAI CASTG 2 cut(s) 87, 873
TseI GCWGC 3 cut(s) 76, 395, 1126
TspDTI ATGAA 6 cut(s) 25, 368, 449, 459, 1010, 1070
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 2 cut(s) 87, 873
Tth111I GACNNNGTC 1 cut(s) 950
Van91I CCANNNNNTGG 2 cut(s) 70, 430
Vha464I CTTAAG 1 cut(s) 737
VpaK11BI GGWCC 2 cut(s) 61, 180
XapI RAATTY 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 3 cut(s) 338, 669, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.