Rh1DG018400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005200
Physical Location & Seq
Reverse (-)
3000778 .. 3014097
13320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG018400.1

Sequence Viewer

Length: 552 bp
ATGGATAATGAGCAAAACGGAAGAGAACGACGACTCGGCGGCTCCGAAGGCGGCGGCGTACTCGAGGCTGACGAGGGCGTCGTCGCTTCAGATGGAGACAGAGGAGGAGTGGAGGAAGAGGAAGGAGTTGCAGACGCTGCTGCGTTTATGCCATTACAAAATGTATTCATTTCCGACATGGACAAAATATGCCATGAATATAAAACAGTACTGACTAATGGACGAAAGAAAGGGGAAAAAACAAAAAGTAACAGGGTAAAAGCCGATCCCAACGACGTCGCCAACCCCCTCCAACAAGTCCACTCCGCTAGCCAGAAATTTGACTATGACCGCCCAGCACCACCCACCATCCATCAAACCGCCGCCGGAACTCCCCTCGGAAACCTACGACCTCAGACAAGAAACCAACATCGAATCACTGTTGCAAATTCTGCCCAGACAAAGAGTGAATGTTTAAAGATAATAGTAACAATGCGCACAGGAAGAATTCAAGATATCCAACCCTTAGAAAACGACCCCCAAATCAAAGTGCAGATACATTACCTATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.15

Weight (kDa)

5.97

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 1 cut(s) 279
Acc16I TGCGCA 1 cut(s) 476
AciI CCGC 7 cut(s) 39, 51, 54, 306, 331, 360, 363
AclWI GGATC 1 cut(s) 260
AcsI RAATTY 3 cut(s) 317, 427, 486
AcuI CTGAAG 1 cut(s) 72
AcyI GRCGYC 2 cut(s) 78, 276
AfaI GTAC 2 cut(s) 60, 210
AgsI TTSAA 1 cut(s) 491
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 1 cut(s) 260
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 137, 140
ApoI RAATTY 3 cut(s) 317, 427, 486
AspLEI GCGC 1 cut(s) 477
AsuNHI GCTAGC 1 cut(s) 308
AvaI CYCGRG 1 cut(s) 62
BbvI GCAGC 2 cut(s) 124, 127
BccI CCATC 3 cut(s) 86, 356, 360
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 309
BisI GCNGC 6 cut(s) 40, 52, 55, 138, 141, 363
BlsI GCNGC 6 cut(s) 41, 53, 56, 139, 142, 364
BmcAI AGTACT 1 cut(s) 210
BmeT110I CYCGRG 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 43
BmtI GCTAGC 1 cut(s) 312
BsaHI GRCGYC 2 cut(s) 78, 276
BsaJI CCNNGG 1 cut(s) 376
BseDI CCNNGG 1 cut(s) 376
BseGI GGATG 1 cut(s) 348
BseMII CTCAG 1 cut(s) 407
BseRI GAGGAG 2 cut(s) 117, 120
BseXI GCAGC 2 cut(s) 124, 127
BseYI CCCAGC 1 cut(s) 334
BsgI GTGCAG 1 cut(s) 551
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 1 cut(s) 366
BsmAI GTCTC 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 62
Bsp143I GATC 1 cut(s) 265
BspACI CCGC 7 cut(s) 39, 51, 54, 306, 331, 360, 363
BspCNI CTCAG 1 cut(s) 406
BspLI GGNNCC 1 cut(s) 43
BspOI GCTAGC 1 cut(s) 312
BspPI GGATC 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 1 cut(s) 265
BssNI GRCGYC 2 cut(s) 78, 276
Bst4CI ACNGT 2 cut(s) 208, 421
Bst6I CTCTTC 2 cut(s) 16, 111
BstACI GRCGYC 2 cut(s) 78, 276
BstAPI GCANNNNNTGC 2 cut(s) 137, 431
BstC8I GCNNGC 1 cut(s) 310
BstDEI CTNAG 2 cut(s) 393, 505
BstF5I GGATG 1 cut(s) 348
BstHHI GCGC 1 cut(s) 477
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 1 cut(s) 265
BstMWI GCNNNNNNNGC 3 cut(s) 48, 137, 431
BstV1I GCAGC 2 cut(s) 124, 127
BtsCI GGATG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 417
Cac8I GCNNGC 1 cut(s) 310
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 1 cut(s) 477
CseI GACGC 2 cut(s) 67, 143
Csp6I GTAC 2 cut(s) 59, 209
CviAII CATG 2 cut(s) 178, 194
CviJI RGCY 4 cut(s) 42, 68, 263, 312
CviKI_1 RGCY 4 cut(s) 42, 68, 263, 312
CviQI GTAC 2 cut(s) 59, 209
DdeI CTNAG 2 cut(s) 393, 505
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
DraI TTTAAA 1 cut(s) 456
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
Eam1104I CTCTTC 2 cut(s) 16, 111
EarI CTCTTC 2 cut(s) 16, 111
Eco32I GATATC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 72
Eco88I CYCGRG 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 486
EcoRV GATATC 1 cut(s) 496
FaeI CATG 2 cut(s) 181, 197
FaiI YATR 7 cut(s) 149, 179, 190, 195, 201, 327, 547
FatI CATG 2 cut(s) 177, 193
Fnu4HI GCNGC 6 cut(s) 40, 52, 55, 138, 141, 363
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 6 cut(s) 40, 52, 55, 138, 141, 363
FspAI RTGCGCAY 1 cut(s) 476
FspBI CTAG 1 cut(s) 309
FspI TGCGCA 1 cut(s) 476
GlaI GCGC 1 cut(s) 476
GluI GCNGC 6 cut(s) 40, 52, 55, 138, 141, 363
GsaI CCCAGC 1 cut(s) 338
HapII CCGG 1 cut(s) 366
HgaI GACGC 2 cut(s) 67, 143
HhaI GCGC 1 cut(s) 477
Hin1I GRCGYC 2 cut(s) 78, 276
Hin1II CATG 2 cut(s) 181, 197
Hin6I GCGC 1 cut(s) 475
HinP1I GCGC 1 cut(s) 475
HinfI GANTC 2 cut(s) 33, 414
HpaII CCGG 1 cut(s) 366
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 5 cut(s) 46, 91, 175, 380, 396
Hpy188III TCNNGA 1 cut(s) 491
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 5 cut(s) 33, 83, 86, 278, 281
HpyAV CCTTC 2 cut(s) 41, 116
HpyCH4III ACNGT 2 cut(s) 208, 421
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 131, 425, 532
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 137, 431
HpyF3I CTNAG 2 cut(s) 393, 505
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 2 cut(s) 78, 276
Hsp92II CATG 2 cut(s) 181, 197
HspAI GCGC 1 cut(s) 475
Kzo9I GATC 1 cut(s) 265
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 6 cut(s) 238, 326, 348, 379, 449, 465
Lsp1109I GCAGC 2 cut(s) 124, 127
MaeI CTAG 1 cut(s) 309
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 248, 466
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 3 cut(s) 33, 128, 495
MluCI AATT 3 cut(s) 317, 427, 486
MlyI GAGTC 1 cut(s) 27
MmeI TCCRAC 3 cut(s) 198, 316, 523
MnlI CCTC 9 cut(s) 58, 67, 95, 98, 106, 112, 299, 386, 402
MseI TTAA 1 cut(s) 455
MspI CCGG 1 cut(s) 366
MwoI GCNNNNNNNGC 3 cut(s) 48, 137, 431
NdeII GATC 1 cut(s) 265
NheI GCTAGC 1 cut(s) 308
NlaIII CATG 2 cut(s) 181, 197
NlaIV GGNNCC 1 cut(s) 43
NmeAIII GCCGAG 1 cut(s) 15
NsbI TGCGCA 1 cut(s) 476
PaeR7I CTCGAG 1 cut(s) 62
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 69, 78
PfeI GAWTC 1 cut(s) 414
PkrI GCNGC 6 cut(s) 41, 53, 56, 139, 142, 364
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 43
PspXI VCTCGAGB 1 cut(s) 62
PstNI CAGNNNCTG 1 cut(s) 137
RsaI GTAC 2 cut(s) 60, 210
RsaNI GTAC 2 cut(s) 59, 209
SaqAI TTAA 1 cut(s) 455
SatI GCNGC 6 cut(s) 40, 52, 55, 138, 141, 363
Sau3AI GATC 1 cut(s) 265
ScaI AGTACT 1 cut(s) 210
SchI GAGTC 1 cut(s) 27
SetI ASST 4 cut(s) 279, 387, 394, 546
Sfr274I CTCGAG 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 3 cut(s) 317, 427, 486
SsiI CCGC 7 cut(s) 39, 51, 54, 306, 331, 360, 363
SspMI CTAG 1 cut(s) 309
TaaI ACNGT 2 cut(s) 208, 421
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 63, 412
TasI AATT 3 cut(s) 317, 427, 486
TatI WGTACW 1 cut(s) 208
TauI GCSGC 4 cut(s) 42, 54, 57, 365
TfiI GAWTC 1 cut(s) 414
Tru1I TTAA 1 cut(s) 455
Tru9I TTAA 1 cut(s) 455
TscAI CASTG 1 cut(s) 424
TseI GCWGC 2 cut(s) 137, 140
TspDTI ATGAA 2 cut(s) 157, 210
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 1 cut(s) 424
XapI RAATTY 3 cut(s) 317, 427, 486
XhoI CTCGAG 1 cut(s) 62
XspI CTAG 1 cut(s) 309
ZraI GACGTC 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.