Rh2BG352500

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005200
Physical Location & Seq
Reverse (-)
48277880 .. 48314668
36789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG352500.1

Sequence Viewer

Length: 822 bp
ATGACTCCTCCCCTACAACAACTTCCGACCTGCTCGTCAATAGGAACATCAGGAAGTAAGTCAACAACTAGGAACATCAGGAAGGAATGGCAGATCGCATTATTGGAATGTGAACTGCTCTACGAGAAAACCTTGAGAAATTGGGATCACCGGCCCTTATCTTGGGAGCACATAACAAATCAGATTGGATCACTGGGAAAACCAAAGTTGGCATTGACTTGGAGGGAAGTGTTTGCAAAGATGTATCAACCTTATAATTTTGGGACCTTCATGTTACTAAGTTCTCTGCTCTTAAATCAAGGGTTACTCTACTGCCATGGACAGGTGTTGGCAACCTGTGGCCCCGGGTGGAGGAATGCAGTTGGAGATAGGGCTGATGTAGAGGATCAACCTCCTGCGATTCCATTCTTCTTTCGTCAGCTGTTGTCAAGTTCTGGAGGTGTAGTCTTGGCTTCTCAAGATGGAAAGATTGTGTGTGAGAACACTCTAGATGCACCGTTGGATATTGTATTCCTTAAAAAACTAACTCAGGAATTATTTTTGCCTTCCATCAGTTTGCCTTCCATAGCCGAAGGAGTAGGTTCACTCTGGATCAGGCCATTAAGAAGAAACTCGATGGAGATTATGAATCAATATTTGGAATTTGTTTTTGTCAACAATTACAAGTACAGAATCCACCCCCGCCACTTGGGGCACCTAGACAGCACAGAATCCTACCCCCACATTGCATTGCGCTACATTGCTCATTTGATTCACAATTTAAATGATAGGCCAATGGAGATTGGGATGGCGTTTGAGAATATGCGTTGTTTTCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
vATP-synt_E

Protein Analysis

273

Amino Acids

31.21

Weight (kDa)

7.68

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_E PF01991 142 - 175 2.2e-06 ATP synthase (E/31 kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
AasI GACNNNNNNGTC 1 cut(s) 34
Acc36I ACCTGC 1 cut(s) 38
AccB1I GGYRCC 1 cut(s) 693
AciI CCGC 1 cut(s) 682
AclWI GGATC 4 cut(s) 153, 196, 393, 599
AcsI RAATTY 1 cut(s) 641
AfaI GTAC 1 cut(s) 668
AfiI CCNNNNNNNGG 4 cut(s) 162, 322, 351, 688
AgsI TTSAA 1 cut(s) 815
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
AluBI AGCT 1 cut(s) 421
AluI AGCT 1 cut(s) 421
Alw21I GWGCWC 1 cut(s) 171
AlwI GGATC 4 cut(s) 153, 196, 393, 599
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 4 cut(s) 152, 340, 596, 770
ApoI RAATTY 1 cut(s) 641
AspLEI GCGC 1 cut(s) 735
AspS9I GGNCC 3 cut(s) 153, 264, 341
AsuC2I CCSGG 2 cut(s) 345, 346
AsuHPI GGTGA 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 1 cut(s) 264
BaeGI GKGCMC 1 cut(s) 696
BanI GGYRCC 1 cut(s) 693
Bbv12I GWGCWC 1 cut(s) 171
BccI CCATC 4 cut(s) 455, 557, 610, 781
BcnI CCSGG 2 cut(s) 345, 346
BfaI CTAG 3 cut(s) 69, 488, 698
BfuAI ACCTGC 1 cut(s) 38
Bme1390I CCNGG 2 cut(s) 345, 346
Bme18I GGWCC 1 cut(s) 264
BmeT110I CYCGRG 1 cut(s) 344
BmgT120I GGNCC 3 cut(s) 153, 264, 341
BmiI GGNNCC 3 cut(s) 265, 343, 695
BmrFI CCNGG 2 cut(s) 345, 346
BmrI ACTGGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 481
BmuI ACTGGG 1 cut(s) 203
BpmI CTGGAG 1 cut(s) 456
BpuEI CTTGAG 2 cut(s) 154, 441
BpuMI CCSGG 2 cut(s) 345, 346
BsaJI CCNNGG 3 cut(s) 316, 343, 344
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 322, 351, 688
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 198
Bse3DI GCAATG 3 cut(s) 723, 728, 738
BseDI CCNNGG 3 cut(s) 316, 343, 344
BseGI GGATG 1 cut(s) 792
BseLI CCNNNNNNNGG 4 cut(s) 162, 322, 351, 688
BseMI GCAATG 3 cut(s) 723, 728, 738
BseMII CTCAG 1 cut(s) 542
BseNI ACTGG 1 cut(s) 198
BseSI GKGCMC 1 cut(s) 696
BshFI GGCC 4 cut(s) 154, 342, 598, 772
BshNI GGYRCC 1 cut(s) 693
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 2 cut(s) 151, 345
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 4 cut(s) 162, 322, 351, 688
BsmFI GGGAC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 4 cut(s) 154, 342, 598, 772
BsoBI CYCGRG 1 cut(s) 344
Bsp1286I GDGCHC 2 cut(s) 171, 696
Bsp143I GATC 5 cut(s) 93, 145, 188, 385, 591
Bsp19I CCATGG 1 cut(s) 316
BspACI CCGC 1 cut(s) 682
BspANI GGCC 4 cut(s) 154, 342, 598, 772
BspCNI CTCAG 1 cut(s) 541
BspLI GGNNCC 3 cut(s) 265, 343, 695
BspMI ACCTGC 1 cut(s) 38
BspPI GGATC 4 cut(s) 153, 196, 393, 599
BspT107I GGYRCC 1 cut(s) 693
BsrDI GCAATG 3 cut(s) 723, 728, 738
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 1 cut(s) 198
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 3 cut(s) 316, 343, 344
BssMI GATC 5 cut(s) 93, 145, 188, 385, 591
BssT1I CCWWGG 1 cut(s) 316
Bst4CI ACNGT 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 278, 528
BstDSI CCRYGG 1 cut(s) 316
BstF5I GGATG 1 cut(s) 792
BstHHI GCGC 1 cut(s) 735
BstKTI GATC 5 cut(s) 96, 148, 191, 388, 594
BstMBI GATC 5 cut(s) 93, 145, 188, 385, 591
BstSCI CCNGG 2 cut(s) 343, 344
BstSLI GKGCMC 1 cut(s) 696
BsuRI GGCC 4 cut(s) 154, 342, 598, 772
BtgI CCRYGG 1 cut(s) 316
BtsCI GGATG 1 cut(s) 792
BtsIMutI CAGTG 1 cut(s) 191
BveI ACCTGC 1 cut(s) 38
CfoI GCGC 1 cut(s) 735
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 3 cut(s) 153, 264, 341
Cfr9I CCCGGG 1 cut(s) 344
Csp6I GTAC 1 cut(s) 667
CviAII CATG 2 cut(s) 271, 317
CviJI RGCY 8 cut(s) 154, 342, 374, 421, 452, 569, 598, 772
CviKI_1 RGCY 8 cut(s) 154, 342, 374, 421, 452, 569, 598, 772
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 2 cut(s) 278, 528
DpnI GATC 5 cut(s) 95, 147, 190, 387, 593
DpnII GATC 5 cut(s) 93, 145, 188, 385, 591
DraI TTTAAA 1 cut(s) 762
DrdI GACNNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 2 cut(s) 274, 320
FaiI YATR 7 cut(s) 173, 255, 272, 318, 566, 626, 803
FaqI GGGAC 1 cut(s) 277
FatI CATG 2 cut(s) 270, 316
FauI CCCGC 1 cut(s) 689
FokI GGATG 1 cut(s) 799
FspBI CTAG 3 cut(s) 69, 488, 698
GlaI GCGC 1 cut(s) 734
GsuI CTGGAG 1 cut(s) 456
HaeIII GGCC 4 cut(s) 154, 342, 598, 772
HapII CCGG 2 cut(s) 151, 345
HhaI GCGC 1 cut(s) 735
Hin1II CATG 2 cut(s) 274, 320
Hin6I GCGC 1 cut(s) 733
HinP1I GCGC 1 cut(s) 733
HincII GTYRAC 2 cut(s) 63, 655
HindII GTYRAC 2 cut(s) 63, 655
HinfI GANTC 6 cut(s) 4, 400, 628, 672, 710, 751
HpaII CCGG 2 cut(s) 151, 345
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 4 cut(s) 63, 113, 584, 655
Hpy188I TCNGA 2 cut(s) 27, 183
Hpy188III TCNNGA 8 cut(s) 51, 79, 435, 458, 488, 530, 589, 815
Hpy8I GTNNAC 4 cut(s) 63, 113, 584, 655
HpyAV CCTTC 5 cut(s) 76, 277, 555, 566, 570
HpyCH4III ACNGT 1 cut(s) 498
HpyCH4V TGCA 4 cut(s) 236, 359, 494, 728
HpyF3I CTNAG 2 cut(s) 278, 528
Hsp92II CATG 2 cut(s) 274, 320
HspAI GCGC 1 cut(s) 733
Kzo9I GATC 5 cut(s) 93, 145, 188, 385, 591
LmnI GCTCC 1 cut(s) 166
LweI GCATC 1 cut(s) 481
MaeI CTAG 3 cut(s) 69, 488, 698
MaeIII GTNAC 2 cut(s) 273, 303
MalI GATC 5 cut(s) 95, 147, 190, 387, 593
MboI GATC 5 cut(s) 93, 145, 188, 385, 591
MboII GAAGA 2 cut(s) 400, 618
MhlI GDGCHC 2 cut(s) 171, 696
MluCI AATT 6 cut(s) 139, 256, 533, 641, 658, 757
MmeI TCCRAC 3 cut(s) 50, 343, 480
MnlI CCTC 6 cut(s) 18, 216, 345, 376, 402, 431
MseI TTAA 4 cut(s) 293, 516, 602, 761
MspA1I CMGCKG 1 cut(s) 421
MspI CCGG 2 cut(s) 151, 345
MspR9I CCNGG 2 cut(s) 345, 346
Mva1269I GAATGC 1 cut(s) 361
NciI CCSGG 2 cut(s) 345, 346
NcoI CCATGG 1 cut(s) 316
NdeII GATC 5 cut(s) 93, 145, 188, 385, 591
NlaIII CATG 2 cut(s) 274, 320
NlaIV GGNNCC 3 cut(s) 265, 343, 695
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 5 cut(s) 400, 628, 672, 710, 751
PpuMI RGGWCCY 1 cut(s) 264
PsiI TTATAA 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 264
PspN4I GGNNCC 3 cut(s) 265, 343, 695
PspPI GGNCC 3 cut(s) 153, 264, 341
PspPPI RGGWCCY 1 cut(s) 264
PvuII CAGCTG 1 cut(s) 421
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
SaqAI TTAA 4 cut(s) 293, 516, 602, 761
Sau3AI GATC 5 cut(s) 93, 145, 188, 385, 591
Sau96I GGNCC 3 cut(s) 153, 264, 341
ScrFI CCNGG 2 cut(s) 345, 346
SduI GDGCHC 2 cut(s) 171, 696
SfaNI GCATC 1 cut(s) 481
SinI GGWCC 1 cut(s) 264
SmaI CCCGGG 1 cut(s) 346
SmiI ATTTAAAT 1 cut(s) 762
SmlI CTYRAG 2 cut(s) 133, 456
SmoI CTYRAG 2 cut(s) 133, 456
Sse9I AATT 6 cut(s) 139, 256, 533, 641, 658, 757
SsiI CCGC 1 cut(s) 682
SspI AATATT 1 cut(s) 635
SspMI CTAG 3 cut(s) 69, 488, 698
StyD4I CCNGG 2 cut(s) 343, 344
StyI CCWWGG 1 cut(s) 316
SwaI ATTTAAAT 1 cut(s) 762
TaaI ACNGT 1 cut(s) 498
TaqI TCGA 1 cut(s) 614
TasI AATT 6 cut(s) 139, 256, 533, 641, 658, 757
TatI WGTACW 1 cut(s) 666
TfiI GAWTC 5 cut(s) 400, 628, 672, 710, 751
Tru1I TTAA 4 cut(s) 293, 516, 602, 761
Tru9I TTAA 4 cut(s) 293, 516, 602, 761
TscAI CASTG 1 cut(s) 198
TspDTI ATGAA 2 cut(s) 259, 641
TspMI CCCGGG 1 cut(s) 344
TspRI CASTG 1 cut(s) 198
VpaK11BI GGWCC 1 cut(s) 264
XapI RAATTY 1 cut(s) 641
XbaI TCTAGA 1 cut(s) 487
XmaI CCCGGG 1 cut(s) 344
XspI CTAG 3 cut(s) 69, 488, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.