Rh2AG017000

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004030
Physical Location & Seq
Forward (+)
1315764 .. 1319326
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG017000.1

Sequence Viewer

Length: 600 bp
ATGGAGAGGAAGGTTGAAGGAGGGGAGCGGGAGATTGAGTGCACGCGCAGAGTGGAGCGTGGTGGAGATCTTTTGGAAGTGACCACTCACAAACGTGCGCGGGTTTGGGCGGTGCGGAACAGTGCTGATAACGGAAATTTACGAATAGCTGCACGGCTCCGTTACGCACGCTCATCTATGAGTGTGAAGCAGCAGAGTGTGCAACCAAGCTGGACCCCTGCACCAGGTAATCAAGTCAAAGTCAATGTTGATGGTGCTTTCTTGCCATCACTTACTAATGGAGGCCTGGGTGAAATTGCACAGGACTCTAAAGTCTGGAACAGGCCCAAAGAGCCATCAAAACCAATTGGTCTACTCTATAAATACCGGGCCTCAAACAACGTAGGGTTTTCTATCGCTAGGGTTTTGTCTGCAAGCCGCTGCTCAGAGCTCCCCACAGCGAAAATGCCGTCGCACAAGACCTTCATCATCAAGAAGAAGCTGGGGAAGAAGATGAGGCAGAACAGGCCCATCCCTCACTGGATCCGCATGAGGACCGACAACACCATCAGGTACAACGCTAAGCGCAGGCACTGGCGCCGCACCAAGCTTGGATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L39

Protein Analysis

199

Amino Acids

22.76

Weight (kDa)

11.29

Isoelectric Point (pI)

55.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L39 PF00832 157 - 197 4.4e-21 Ribosomal L39 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 576
AccBSI CCGCTC 1 cut(s) 28
AccI GTMKAC 1 cut(s) 352
AccII CGCG 2 cut(s) 46, 100
AciI CCGC 7 cut(s) 28, 100, 110, 115, 418, 526, 580
AclWI GGATC 2 cut(s) 517, 530
AcsI RAATTY 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 577
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 224
AgsI TTSAA 1 cut(s) 17
AjnI CCWGG 2 cut(s) 223, 285
AjuI GAANNNNNNNTTGG 1 cut(s) 578
AleI CACNNNNGTG 1 cut(s) 93
AluBI AGCT 5 cut(s) 149, 210, 430, 481, 589
AluI AGCT 5 cut(s) 149, 210, 430, 481, 589
Alw21I GWGCWC 2 cut(s) 44, 432
Alw44I GTGCAC 1 cut(s) 40
AlwI GGATC 2 cut(s) 517, 530
AlwNI CAGNNNCTG 1 cut(s) 573
AoxI GGCC 4 cut(s) 283, 323, 369, 506
ApaLI GTGCAC 1 cut(s) 40
ApeKI GCWGC 3 cut(s) 149, 190, 420
ApoI RAATTY 1 cut(s) 136
ArsI GACNNNNNNTTYG 2 cut(s) 434, 466
AspLEI GCGC 4 cut(s) 48, 100, 567, 579
AspS9I GGNCC 5 cut(s) 213, 324, 369, 507, 534
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 302
AvaII GGWCC 2 cut(s) 213, 534
BaeGI GKGCMC 1 cut(s) 44
BamHI GGATCC 1 cut(s) 522
BanI GGYRCC 1 cut(s) 576
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 44, 432
BbvI GCAGC 3 cut(s) 136, 202, 407
BccI CCATC 5 cut(s) 245, 274, 343, 518, 554
BceAI ACGGC 2 cut(s) 170, 433
BciT130I CCWGG 2 cut(s) 225, 287
BcnI CCSGG 1 cut(s) 368
BfaI CTAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 580
BglII AGATCT 1 cut(s) 67
BisI GCNGC 5 cut(s) 150, 191, 418, 421, 580
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 5 cut(s) 151, 192, 419, 422, 581
Bme1390I CCNGG 3 cut(s) 225, 287, 368
Bme18I GGWCC 2 cut(s) 213, 534
BmgT120I GGNCC 5 cut(s) 213, 324, 369, 507, 534
BmiI GGNNCC 4 cut(s) 158, 215, 524, 578
BmrFI CCNGG 3 cut(s) 225, 287, 368
Bpu1102I GCTNAGC 1 cut(s) 561
BpuMI CCSGG 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 577
BsaJI CCNNGG 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 1 cut(s) 224
Bse1I ACTGG 2 cut(s) 524, 578
BseBI CCWGG 2 cut(s) 225, 287
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 1 cut(s) 510
BseLI CCNNNNNNNGG 1 cut(s) 224
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 2 cut(s) 524, 578
BseSI GKGCMC 1 cut(s) 44
BseXI GCAGC 3 cut(s) 136, 202, 407
BseYI CCCAGC 1 cut(s) 481
BsgI GTGCAG 2 cut(s) 135, 204
Bsh1236I CGCG 2 cut(s) 46, 100
BshFI GGCC 4 cut(s) 285, 325, 371, 508
BshNI GGYRCC 1 cut(s) 576
BsiHKAI GWGCWC 2 cut(s) 44, 432
BsiSI CCGG 1 cut(s) 367
BslI CCNNNNNNNGG 1 cut(s) 224
BsnI GGCC 4 cut(s) 285, 325, 371, 508
Bsp1286I GDGCHC 2 cut(s) 44, 432
Bsp143I GATC 2 cut(s) 67, 522
Bsp1720I GCTNAGC 1 cut(s) 561
BspACI CCGC 7 cut(s) 28, 100, 110, 115, 418, 526, 580
BspANI GGCC 4 cut(s) 285, 325, 371, 508
BspCNI CTCAG 1 cut(s) 437
BspFNI CGCG 2 cut(s) 46, 100
BspLI GGNNCC 4 cut(s) 158, 215, 524, 578
BspPI GGATC 2 cut(s) 517, 530
BspT107I GGYRCC 1 cut(s) 576
BsrBI CCGCTC 1 cut(s) 28
BsrI ACTGG 2 cut(s) 524, 578
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 2 cut(s) 67, 522
BssNI GRCGYC 1 cut(s) 577
Bst2UI CCWGG 2 cut(s) 225, 287
Bst4CI ACNGT 1 cut(s) 122
BstACI GRCGYC 1 cut(s) 577
BstAPI GCANNNNNTGC 1 cut(s) 199
BstC8I GCNNGC 4 cut(s) 44, 169, 415, 569
BstDEI CTNAG 2 cut(s) 424, 561
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 510
BstFNI CGCG 2 cut(s) 46, 100
BstH2I RGCGCY 1 cut(s) 580
BstHHI GCGC 4 cut(s) 48, 100, 567, 579
BstKTI GATC 2 cut(s) 70, 525
BstMBI GATC 2 cut(s) 67, 522
BstMWI GCNNNNNNNGC 3 cut(s) 199, 331, 505
BstNI CCWGG 2 cut(s) 225, 287
BstSCI CCNGG 3 cut(s) 223, 285, 366
BstSLI GKGCMC 1 cut(s) 44
BstUI CGCG 2 cut(s) 46, 100
BstV1I GCAGC 3 cut(s) 136, 202, 407
BstX2I RGATCY 2 cut(s) 67, 522
BstYI RGATCY 2 cut(s) 67, 522
BsuRI GGCC 4 cut(s) 285, 325, 371, 508
BtsCI GGATG 1 cut(s) 510
BtsIMutI CAGTG 3 cut(s) 127, 517, 571
Cac8I GCNNGC 4 cut(s) 44, 169, 415, 569
CaiI CAGNNNCTG 1 cut(s) 573
CfoI GCGC 4 cut(s) 48, 100, 567, 579
Cfr13I GGNCC 5 cut(s) 213, 324, 369, 507, 534
CsiI ACCWGGT 1 cut(s) 223
Csp6I GTAC 1 cut(s) 553
CviAII CATG 1 cut(s) 529
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 2 cut(s) 424, 561
DinI GGCGCC 1 cut(s) 578
DpnI GATC 2 cut(s) 69, 524
DpnII GATC 2 cut(s) 67, 522
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
Ecl136II GAGCTC 1 cut(s) 430
Eco147I AGGCCT 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 432
Eco47I GGWCC 2 cut(s) 213, 534
Eco53kI GAGCTC 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoRII CCWGG 2 cut(s) 223, 285
EcoT38I GRGCYC 1 cut(s) 432
EgeI GGCGCC 1 cut(s) 578
EheI GGCGCC 1 cut(s) 578
FaeI CATG 1 cut(s) 532
FaiI YATR 3 cut(s) 179, 360, 530
FatI CATG 1 cut(s) 528
FauI CCCGC 2 cut(s) 21, 93
FblI GTMKAC 1 cut(s) 352
Fnu4HI GCNGC 5 cut(s) 150, 191, 418, 421, 580
FokI GGATG 1 cut(s) 497
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 5 cut(s) 150, 191, 418, 421, 580
FspBI CTAG 1 cut(s) 399
GlaI GCGC 4 cut(s) 47, 99, 566, 578
GluI GCNGC 5 cut(s) 150, 191, 418, 421, 580
GsaI CCCAGC 1 cut(s) 485
HaeII RGCGCY 1 cut(s) 580
HaeIII GGCC 4 cut(s) 285, 325, 371, 508
HapII CCGG 1 cut(s) 367
HhaI GCGC 4 cut(s) 48, 100, 567, 579
Hin1I GRCGYC 1 cut(s) 577
Hin1II CATG 1 cut(s) 532
Hin6I GCGC 4 cut(s) 46, 98, 565, 577
HinP1I GCGC 4 cut(s) 46, 98, 565, 577
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 2 cut(s) 305, 594
HpaII CCGG 1 cut(s) 367
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 2 cut(s) 42, 353
Hpy188I TCNGA 1 cut(s) 427
Hpy188III TCNNGA 2 cut(s) 316, 472
Hpy8I GTNNAC 2 cut(s) 42, 353
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 3 cut(s) 4, 11, 472
HpyCH4III ACNGT 1 cut(s) 122
HpyCH4IV ACGT 2 cut(s) 94, 381
HpyCH4V TGCA 6 cut(s) 42, 152, 202, 221, 299, 413
HpyF10VI GCNNNNNNNGC 3 cut(s) 199, 331, 505
HpyF3I CTNAG 2 cut(s) 424, 561
HpySE526I ACGT 2 cut(s) 94, 381
Hsp92I GRCGYC 1 cut(s) 577
Hsp92II CATG 1 cut(s) 532
HspAI GCGC 4 cut(s) 46, 98, 565, 577
KasI GGCGCC 1 cut(s) 576
Kzo9I GATC 2 cut(s) 67, 522
LmnI GCTCC 4 cut(s) 25, 55, 162, 435
Lsp1109I GCAGC 3 cut(s) 136, 202, 407
MabI ACCWGGT 1 cut(s) 223
MaeI CTAG 1 cut(s) 399
MaeII ACGT 2 cut(s) 94, 381
MaeIII GTNAC 2 cut(s) 79, 161
MalI GATC 2 cut(s) 69, 524
MbiI CCGCTC 1 cut(s) 28
MboI GATC 2 cut(s) 67, 522
MboII GAAGA 3 cut(s) 487, 499, 502
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 2 cut(s) 67, 522
MhlI GDGCHC 2 cut(s) 44, 432
MluCI AATT 3 cut(s) 136, 294, 345
Mly113I GGCGCC 1 cut(s) 577
MlyI GAGTC 1 cut(s) 299
MnlI CCTC 6 cut(s) 14, 275, 382, 489, 525, 525
MslI CAYNNNNRTG 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 420
MspI CCGG 1 cut(s) 367
MspR9I CCNGG 3 cut(s) 225, 287, 368
MunI CAATTG 1 cut(s) 345
MvaI CCWGG 2 cut(s) 225, 287
MvnI CGCG 2 cut(s) 46, 100
MwoI GCNNNNNNNGC 3 cut(s) 199, 331, 505
NarI GGCGCC 1 cut(s) 577
NciI CCSGG 1 cut(s) 368
NdeII GATC 2 cut(s) 67, 522
NlaIII CATG 1 cut(s) 532
NlaIV GGNNCC 4 cut(s) 158, 215, 524, 578
NmuCI GTSAC 1 cut(s) 79
OliI CACNNNNGTG 1 cut(s) 93
PceI AGGCCT 1 cut(s) 285
PfeI GAWTC 1 cut(s) 594
PkrI GCNGC 5 cut(s) 151, 192, 419, 422, 581
PleI GAGTC 1 cut(s) 299
PluTI GGCGCC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 223, 285
PspFI CCCAGC 1 cut(s) 481
PspGI CCWGG 2 cut(s) 223, 285
PspN4I GGNNCC 4 cut(s) 158, 215, 524, 578
PspPI GGNCC 5 cut(s) 213, 324, 369, 507, 534
PstNI CAGNNNCTG 1 cut(s) 573
PsuI RGATCY 2 cut(s) 67, 522
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 93
SacI GAGCTC 1 cut(s) 432
SatI GCNGC 5 cut(s) 150, 191, 418, 421, 580
Sau3AI GATC 2 cut(s) 67, 522
Sau96I GGNCC 5 cut(s) 213, 324, 369, 507, 534
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 3 cut(s) 225, 287, 368
SduI GDGCHC 2 cut(s) 44, 432
SexAI ACCWGGT 1 cut(s) 223
SfoI GGCGCC 1 cut(s) 578
SinI GGWCC 2 cut(s) 213, 534
SmiMI CAYNNNNRTG 1 cut(s) 93
Sse9I AATT 3 cut(s) 136, 294, 345
SseBI AGGCCT 1 cut(s) 285
SsiI CCGC 7 cut(s) 28, 100, 110, 115, 418, 526, 580
SspDI GGCGCC 1 cut(s) 576
SspMI CTAG 1 cut(s) 399
SstI GAGCTC 1 cut(s) 432
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 223, 285, 366
TaaI ACNGT 1 cut(s) 122
TaiI ACGT 2 cut(s) 97, 384
TaqII GACCGA 1 cut(s) 551
TasI AATT 3 cut(s) 136, 294, 345
TauI GCSGC 2 cut(s) 420, 582
TfiI GAWTC 1 cut(s) 594
TscAI CASTG 3 cut(s) 127, 524, 578
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 3 cut(s) 149, 190, 420
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 1 cut(s) 454
TspGWI ACGGA 2 cut(s) 147, 149
TspRI CASTG 3 cut(s) 127, 524, 578
VneI GTGCAC 1 cut(s) 40
VpaK11BI GGWCC 2 cut(s) 213, 534
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 352
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.