Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0003967

Physical Location & Seq

Genomic Coordinates Forward (+)

36139750 .. 36141178

Gene Structure 1429 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG284100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGCGTACCCCACTTGTTTACCATTCTACTCCTTCAATTCCAACCTTTCAGTCACCTTCAGTCTCCACCTTTGTGCCCCAACTCCAATTTACTGATCCAAAACCTCTTCCTTTCTATAGCAAGCCGTTTCGGGGTTTCAGATGTAGAGTCGTCATGGGTGCAAAAGCAAGAGTTGCCAAAAAGACCCGAAACCCAGATCTGCTCCGTGATATGGGTAAGTATTCGAGGTCCAAGAGGTACCACAGGTGCGGTCTCTGGGTCATCAAGGCCAGAAACGACGATGTATTCCCACGCCACAATGCCAAGGCGGCAGTTGCAATTATGGAGGAGCTTCATCAACTACTTGTGGGTCAAAAAACTCATATTACTGAGCCGGCCTTGAGGCCATTGAAGTTTGGGTCACTACCAGCTCCTATGGTTGCTGCGTCTCTTACGGCGTCGGGGAAGACATCGTCCTCAGCTACGGCTATAGCTGGAGGTAGTATTCGAATCTCAGAACCAAATTTTAATATATCACCTCATGAAGCAATATTGGATTTGAGTAATGATATTTCTGTTGTGGTATCTGGAAGTAGTAGTGGGGATAATATTGTTGTTGGTATGATTGAAAAACTTGTGTTGAATGTTGATAAAAATATTGTTGGAGGTGTTACTGAGAAATTGAATATTGTGAGTAACAGCATGGGTGCTAGTACGGAAAATAGGGAGTCTGAGATTCGAAGTACTGAAGGGGCAATTGTTACAGTAAAATCAATTGGGAAGCTGCGAGATGGTAAGGAATTTTTTAAGAAAGGTCATGGTGAAGGAGAAGCACCGTTTGAGTTCAAGAAAGACGCGGATCAAGTGATTGAGGAGCTCGATAAAGCTGTAGTGACAATGAGCAAGGTTTTAGATGAAGCAAGTCTTCCTTCTAACTTCATAGATACTATAATGGATGGCGTTCTTTACTCAATAGAGGCTGTTACATGCATCCCACAGTGTCTATATGCCATGTGCTTAAATAATAATTGTTTTCAGGCTATGAAAGATCGCAATGCTTTGAGGTGCTTTGTGAAGTGTACTTCAGGAACCTATTTGCGTGCTCTAACAAGTGATAAATCGATCTCCTTCTCTAGTGGATTGGGTGAATTATTGCGTCATGCTTCCTCATTACATGGTCCTCATCTGGATATGCTAATTGAGATTTTAAAGGCCATCTCAAATATTCAACACAGGGTTGATGCTTCTCTCACGTCAACTGAACTTTTGTCTTCTTTTATTCCTGTTATTATGAAAACTGATGGAGGAGAGAGGAATTTAGTCATGACAGATGATAGGAAGTCGTTCAAATGGATAGTTTGGAGCAGGGAACTGAACCATCTTCTGATTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0005911 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

DUF4414 DUF908 DUF913 HECT UBA

Protein Analysis

457

Amino Acids

50.27

Weight (kDa)

9.04

Isoelectric Point (pI)

43.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribosomal_L6e_N	PF03868	55 - 102	3.4e-06	Ribosomal protein L6, N-terminal domain
DUF913	PF06025	292 - 407	1e-20	Domain of Unknown Function (DUF913)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003967)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr2g0101351 RchiOBHm_Chr4g0387901 RchiOBHm_Chr7g0182321 RchiOBHm_Chr7g0194411
rosa_laevigata	RLG00000004201 RLG00000004202 RLG00000022917
rosa_multiflora	Rmu_sc0002837.1_g000001 Rmu_sc0013203.1_g000002
rosa_roxburghii	Rroxscaffold_1G00009390 Rroxscaffold_1G00019360 Rroxscaffold_1G00050800 Rroxscaffold_2G00084810 Rroxscaffold_2G00130580 Rroxscaffold_2G00141360 Rroxscaffold_3G00222730 Rroxscaffold_3G00261360
rosa_rugosa	Rorug07G0015700
rosa_samantha	Rh2AG146200 Rh2AG296300 Rh2BG151600 Rh2CG152200 Rh2DG151700 Rh4AG019200 Rh5BG284100 Rh5BG303900 Rh6AG185400 Rh6AG328900 Rh7AG049900 Rh7AG142900 Rh7BG143400 Rh7CG147200 Rh7DG144800
rosa_wichuraiana	Rw7G012230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	237
AccB1I	GGYRCC	1 cut(s)	237
AccII	CGCG	1 cut(s)	836
AciI	CCGC	3 cut(s)	249, 308, 836
AclWI	GGATC	2 cut(s)	89, 846
AcsI	RAATTY	3 cut(s)	502, 779, 1294
AcuI	CTGAAG	3 cut(s)	42, 747, 1047
AcyI	GRCGYC	1 cut(s)	437
AfaI	GTAC	5 cut(s)	7, 239, 694, 724, 1060
AfiI	CCNNNNNNNGG	2 cut(s)	131, 211
AgsI	TTSAA	8 cut(s)	36, 391, 608, 622, 664, 826, 1208, 1327
AjiI	CACGTC	1 cut(s)	1233
AleI	CACNNNNGTG	1 cut(s)	71
AluBI	AGCT	7 cut(s)	331, 410, 461, 473, 763, 856, 866
AluI	AGCT	7 cut(s)	331, 410, 461, 473, 763, 856, 866
Alw21I	GWGCWC	2 cut(s)	858, 1084
Alw26I	GTCTC	3 cut(s)	67, 257, 432
AlwI	GGATC	2 cut(s)	89, 846
AoxI	GGCC	4 cut(s)	267, 375, 383, 1191
ApeKI	GCWGC	2 cut(s)	422, 763
ApoI	RAATTY	3 cut(s)	502, 779, 1294
Asp700I	GAANNNNTTC	1 cut(s)	1322
Asp718I	GGTACC	1 cut(s)	237
AspS9I	GGNCC	2 cut(s)	228, 1157
AsuHPI	GGTGA	4 cut(s)	45, 507, 812, 1136
AsuII	TTCGAA	2 cut(s)	487, 718
AvaII	GGWCC	2 cut(s)	228, 1157
BaeGI	GKGCMC	1 cut(s)	78
BanI	GGYRCC	1 cut(s)	237
BanII	GRGCYC	1 cut(s)	858
BbsI	GAAGAC	3 cut(s)	452, 896, 1242
Bbv12I	GWGCWC	2 cut(s)	858, 1084
BbvCI	CCTCAGC	1 cut(s)	457
BbvI	GCAGC	2 cut(s)	409, 750
BccI	CCATC	5 cut(s)	764, 929, 1202, 1274, 1365
BceAI	ACGGC	3 cut(s)	109, 450, 480
BcoDI	GTCTC	3 cut(s)	67, 257, 432
BfaI	CTAG	2 cut(s)	690, 1113
BfmI	CTRYAG	3 cut(s)	115, 468, 867
BglI	GCCNNNNNGGC	1 cut(s)	308
BglII	AGATCT	1 cut(s)	196
BisI	GCNGC	3 cut(s)	309, 423, 764
BlsI	GCNGC	3 cut(s)	310, 424, 765
BmcAI	AGTACT	1 cut(s)	724
Bme18I	GGWCC	2 cut(s)	228, 1157
BmgBI	CACGTC	1 cut(s)	1233
BmgT120I	GGNCC	2 cut(s)	228, 1157
BmiI	GGNNCC	2 cut(s)	239, 1069
BmsI	GCATC	2 cut(s)	978, 1210
BpiI	GAAGAC	3 cut(s)	452, 896, 1242
BpmI	CTGGAG	1 cut(s)	495
Bpu10I	CCTNAGC	1 cut(s)	457
Bpu14I	TTCGAA	2 cut(s)	487, 718
BpuEI	CTTGAG	1 cut(s)	400
Bsa29I	ATCGAT	1 cut(s)	1100
BsaHI	GRCGYC	1 cut(s)	437
BsaI	GGTCTC	1 cut(s)	257
BsaJI	CCNNGG	1 cut(s)	303
Bsc4I	CCNNNNNNNGG	2 cut(s)	131, 211
Bse118I	RCCGGY	1 cut(s)	373
Bse3DI	GCAATG	1 cut(s)	1039
BseCI	ATCGAT	1 cut(s)	1100
BseDI	CCNNGG	1 cut(s)	303
BseGI	GGATG	2 cut(s)	940, 969
BseLI	CCNNNNNNNGG	2 cut(s)	131, 211
BseMI	GCAATG	1 cut(s)	1039
BseMII	CTCAG	5 cut(s)	360, 471, 507, 645, 702
BseRI	GAGGAG	3 cut(s)	341, 866, 1299
BseSI	GKGCMC	1 cut(s)	78
BseXI	GCAGC	2 cut(s)	409, 750
Bsh1236I	CGCG	1 cut(s)	836
BshFI	GGCC	4 cut(s)	269, 377, 385, 1193
BshNI	GGYRCC	1 cut(s)	237
BshVI	ATCGAT	1 cut(s)	1100
BsiHKAI	GWGCWC	2 cut(s)	858, 1084
BsiSI	CCGG	1 cut(s)	374
BslI	CCNNNNNNNGG	2 cut(s)	131, 211
BsmAI	GTCTC	3 cut(s)	67, 257, 432
BsmBI	CGTCTC	1 cut(s)	432
BsnI	GGCC	4 cut(s)	269, 377, 385, 1193
Bso31I	GGTCTC	1 cut(s)	257
Bsp119I	TTCGAA	2 cut(s)	487, 718
Bsp1286I	GDGCHC	3 cut(s)	78, 858, 1084
Bsp143I	GATC	5 cut(s)	94, 196, 838, 1027, 1101
BspACI	CCGC	3 cut(s)	249, 308, 836
BspANI	GGCC	4 cut(s)	269, 377, 385, 1193
BspCNI	CTCAG	5 cut(s)	361, 470, 506, 646, 703
BspDI	ATCGAT	1 cut(s)	1100
BspFNI	CGCG	1 cut(s)	836
BspHI	TCATGA	2 cut(s)	520, 1302
BspLI	GGNNCC	2 cut(s)	239, 1069
BspPI	GGATC	2 cut(s)	89, 846
BspT104I	TTCGAA	2 cut(s)	487, 718
BspT107I	GGYRCC	1 cut(s)	237
BspTNI	GGTCTC	1 cut(s)	257
BsrDI	GCAATG	1 cut(s)	1039
BsrFI	RCCGGY	1 cut(s)	373
BssAI	RCCGGY	1 cut(s)	373
BssECI	CCNNGG	1 cut(s)	303
BssMI	GATC	5 cut(s)	94, 196, 838, 1027, 1101
BssNI	GRCGYC	1 cut(s)	437
BssT1I	CCWWGG	1 cut(s)	303
Bst4CI	ACNGT	3 cut(s)	745, 816, 978
Bst6I	CTCTTC	1 cut(s)	111
BstACI	GRCGYC	1 cut(s)	437
BstAPI	GCANNNNNTGC	1 cut(s)	173
BstBI	TTCGAA	2 cut(s)	487, 718
BstC8I	GCNNGC	3 cut(s)	122, 375, 1080
BstDEI	CTNAG	5 cut(s)	369, 457, 493, 654, 711
BstF5I	GGATG	2 cut(s)	940, 969
BstFNI	CGCG	1 cut(s)	836
BstKTI	GATC	5 cut(s)	97, 199, 841, 1030, 1104
BstMAI	GTCTC	3 cut(s)	67, 257, 432
BstMBI	GATC	5 cut(s)	94, 196, 838, 1027, 1101
BstMWI	GCNNNNNNNGC	3 cut(s)	173, 308, 314
BstNSI	RCATGY	1 cut(s)	969
BstSFI	CTRYAG	3 cut(s)	115, 468, 867
BstSLI	GKGCMC	1 cut(s)	78
BstUI	CGCG	1 cut(s)	836
BstV1I	GCAGC	2 cut(s)	409, 750
BstV2I	GAAGAC	3 cut(s)	452, 896, 1242
BstX2I	RGATCY	1 cut(s)	196
BstYI	RGATCY	1 cut(s)	196
Bsu15I	ATCGAT	1 cut(s)	1100
BsuRI	GGCC	4 cut(s)	269, 377, 385, 1193
BsuTUI	ATCGAT	1 cut(s)	1100
BtrI	CACGTC	1 cut(s)	1233
BtsCI	GGATG	2 cut(s)	940, 969
BtsIMutI	CAGTG	1 cut(s)	983
Cac8I	GCNNGC	3 cut(s)	122, 375, 1080
CciI	TCATGA	2 cut(s)	520, 1302
Cfr10I	RCCGGY	1 cut(s)	373
Cfr13I	GGNCC	2 cut(s)	228, 1157
ClaI	ATCGAT	1 cut(s)	1100
CseI	GACGC	4 cut(s)	414, 426, 842, 1124
Csp6I	GTAC	5 cut(s)	6, 238, 693, 723, 1059
CviAII	CATG	9 cut(s)	154, 521, 682, 797, 966, 991, 1139, 1154, 1303
CviQI	GTAC	5 cut(s)	6, 238, 693, 723, 1059
DdeI	CTNAG	5 cut(s)	369, 457, 493, 654, 711
DpnI	GATC	5 cut(s)	96, 198, 840, 1029, 1103
DpnII	GATC	5 cut(s)	94, 196, 838, 1027, 1101
DraI	TTTAAA	1 cut(s)	1188
Eam1104I	CTCTTC	1 cut(s)	111
EarI	CTCTTC	1 cut(s)	111
Ecl136II	GAGCTC	1 cut(s)	856
Eco130I	CCWWGG	1 cut(s)	303
Eco24I	GRGCYC	1 cut(s)	858
Eco31I	GGTCTC	1 cut(s)	257
Eco47I	GGWCC	2 cut(s)	228, 1157
Eco53kI	GAGCTC	1 cut(s)	856
Eco57I	CTGAAG	3 cut(s)	42, 747, 1047
EcoICRI	GAGCTC	1 cut(s)	856
EcoT14I	CCWWGG	1 cut(s)	303
EcoT22I	ATGCAT	1 cut(s)	971
EcoT38I	GRGCYC	1 cut(s)	858
ErhI	CCWWGG	1 cut(s)	303
Esp3I	CGTCTC	1 cut(s)	432
FaeI	CATG	9 cut(s)	157, 524, 685, 800, 969, 994, 1142, 1157, 1306
FalI	AAGNNNNNCTT	4 cut(s)	888, 920, 892, 924
FatI	CATG	9 cut(s)	153, 520, 681, 796, 965, 990, 1138, 1153, 1302
Fnu4HI	GCNGC	3 cut(s)	309, 423, 764
FokI	GGATG	2 cut(s)	947, 956
FriOI	GRGCYC	1 cut(s)	858
Fsp4HI	GCNGC	3 cut(s)	309, 423, 764
FspBI	CTAG	2 cut(s)	690, 1113
GluI	GCNGC	3 cut(s)	309, 423, 764
GsuI	CTGGAG	1 cut(s)	495
HaeIII	GGCC	4 cut(s)	269, 377, 385, 1193
HapII	CCGG	1 cut(s)	374
HgaI	GACGC	4 cut(s)	414, 426, 842, 1124
Hin1I	GRCGYC	1 cut(s)	437
Hin1II	CATG	9 cut(s)	157, 524, 685, 800, 969, 994, 1142, 1157, 1306
HincII	GTYRAC	1 cut(s)	1236
HindII	GTYRAC	1 cut(s)	1236
HinfI	GANTC	5 cut(s)	147, 489, 707, 715, 1366
HpaII	CCGG	1 cut(s)	374
HphI	GGTGA	4 cut(s)	45, 507, 812, 1136
Hpy166II	GTNNAC	3 cut(s)	19, 1059, 1236
Hpy188I	TCNGA	4 cut(s)	140, 496, 712, 1365
Hpy188III	TCNNGA	6 cut(s)	521, 567, 826, 1065, 1166, 1303
Hpy8I	GTNNAC	3 cut(s)	19, 1059, 1236
Hpy99I	CGWCG	2 cut(s)	281, 442
HpyAV	CCTTC	6 cut(s)	42, 66, 722, 797, 918, 1117
HpyCH4III	ACNGT	3 cut(s)	745, 816, 978
HpyCH4IV	ACGT	1 cut(s)	1232
HpyCH4V	TGCA	3 cut(s)	161, 317, 969
HpyF10VI	GCNNNNNNNGC	3 cut(s)	173, 308, 314
HpyF3I	CTNAG	5 cut(s)	369, 457, 493, 654, 711
HpySE526I	ACGT	1 cut(s)	1232
Hsp92I	GRCGYC	1 cut(s)	437
Hsp92II	CATG	9 cut(s)	157, 524, 685, 800, 969, 994, 1142, 1157, 1306
KpnI	GGTACC	1 cut(s)	241
KroI	GCCGGC	1 cut(s)	373
KroNI	GCCGGC	1 cut(s)	375
Kzo9I	GATC	5 cut(s)	94, 196, 838, 1027, 1101
LmnI	GCTCC	5 cut(s)	207, 328, 415, 853, 1341
Lsp1109I	GCAGC	2 cut(s)	409, 750
LweI	GCATC	2 cut(s)	978, 1210
MaeI	CTAG	2 cut(s)	690, 1113
MaeII	ACGT	1 cut(s)	1232
MaeIII	GTNAC	7 cut(s)	51, 399, 649, 674, 739, 871, 961
MalI	GATC	5 cut(s)	96, 198, 840, 1029, 1103
MboI	GATC	5 cut(s)	94, 196, 838, 1027, 1101
MboII	GAAGA	5 cut(s)	98, 457, 896, 1242, 1352
MfeI	CAATTG	2 cut(s)	735, 753
MflI	RGATCY	1 cut(s)	196
MhlI	GDGCHC	3 cut(s)	78, 858, 1084
MlyI	GAGTC	2 cut(s)	156, 716
MmeI	TCCRAC	2 cut(s)	65, 622
Mph1103I	ATGCAT	1 cut(s)	971
MroNI	GCCGGC	1 cut(s)	373
MroXI	GAANNNNTTC	1 cut(s)	1322
MseI	TTAA	4 cut(s)	507, 786, 998, 1187
MslI	CAYNNNNRTG	1 cut(s)	71
MspI	CCGG	1 cut(s)	374
MunI	CAATTG	2 cut(s)	735, 753
MvnI	CGCG	1 cut(s)	836
MwoI	GCNNNNNNNGC	3 cut(s)	173, 308, 314
NaeI	GCCGGC	1 cut(s)	375
NdeII	GATC	5 cut(s)	94, 196, 838, 1027, 1101
NgoMIV	GCCGGC	1 cut(s)	373
NlaIII	CATG	9 cut(s)	157, 524, 685, 800, 969, 994, 1142, 1157, 1306
NlaIV	GGNNCC	2 cut(s)	239, 1069
NmuCI	GTSAC	3 cut(s)	51, 399, 871
NsiI	ATGCAT	1 cut(s)	971
NspI	RCATGY	1 cut(s)	969
NspV	TTCGAA	2 cut(s)	487, 718
OliI	CACNNNNGTG	1 cut(s)	71
PagI	TCATGA	2 cut(s)	520, 1302
PdiI	GCCGGC	1 cut(s)	375
PdmI	GAANNNNTTC	1 cut(s)	1322
PfeI	GAWTC	3 cut(s)	489, 715, 1366
PflFI	GACNNNGTC	1 cut(s)	451
PkrI	GCNGC	3 cut(s)	310, 424, 765
PleI	GAGTC	2 cut(s)	155, 715
PpsI	GAGTC	2 cut(s)	155, 715
Psp124BI	GAGCTC	1 cut(s)	858
PspN4I	GGNNCC	2 cut(s)	239, 1069
PspPI	GGNCC	2 cut(s)	228, 1157
PsuI	RGATCY	1 cut(s)	196
PsyI	GACNNNGTC	1 cut(s)	451
RsaI	GTAC	5 cut(s)	7, 239, 694, 724, 1060
RsaNI	GTAC	5 cut(s)	6, 238, 693, 723, 1059
RseI	CAYNNNNRTG	1 cut(s)	71
SacI	GAGCTC	1 cut(s)	858
SaqAI	TTAA	4 cut(s)	507, 786, 998, 1187
SatI	GCNGC	3 cut(s)	309, 423, 764
Sau3AI	GATC	5 cut(s)	94, 196, 838, 1027, 1101
Sau96I	GGNCC	2 cut(s)	228, 1157
ScaI	AGTACT	1 cut(s)	724
SchI	GAGTC	2 cut(s)	156, 716
SduI	GDGCHC	3 cut(s)	78, 858, 1084
SfaNI	GCATC	2 cut(s)	978, 1210
SfcI	CTRYAG	3 cut(s)	115, 468, 867
SfuI	TTCGAA	2 cut(s)	487, 718
SinI	GGWCC	2 cut(s)	228, 1157
SmiMI	CAYNNNNRTG	1 cut(s)	71
SmlI	CTYRAG	1 cut(s)	379
SmoI	CTYRAG	1 cut(s)	379
SsiI	CCGC	3 cut(s)	249, 308, 836
SspI	AATATT	5 cut(s)	531, 589, 637, 667, 1204
SspMI	CTAG	2 cut(s)	690, 1113
SstI	GAGCTC	1 cut(s)	858
StyI	CCWWGG	1 cut(s)	303
TaaI	ACNGT	3 cut(s)	745, 816, 978
TaiI	ACGT	1 cut(s)	1235
TaqI	TCGA	5 cut(s)	224, 487, 718, 858, 1100
TatI	WGTACW	2 cut(s)	722, 1058
TauI	GCSGC	1 cut(s)	311
TfiI	GAWTC	3 cut(s)	489, 715, 1366
Tru1I	TTAA	4 cut(s)	507, 786, 998, 1187
Tru9I	TTAA	4 cut(s)	507, 786, 998, 1187
TscAI	CASTG	1 cut(s)	983
TseFI	GTSAC	3 cut(s)	51, 399, 871
TseI	GCWGC	2 cut(s)	422, 763
Tsp45I	GTSAC	3 cut(s)	51, 399, 871
TspDTI	ATGAA	6 cut(s)	323, 537, 907, 909, 1037, 1286
TspGWI	ACGGA	2 cut(s)	194, 710
TspRI	CASTG	1 cut(s)	983
Tth111I	GACNNNGTC	1 cut(s)	451
VpaK11BI	GGWCC	2 cut(s)	228, 1157
XapI	RAATTY	3 cut(s)	502, 779, 1294
XceI	RCATGY	1 cut(s)	969
XmnI	GAANNNNTTC	1 cut(s)	1322
XspI	CTAG	2 cut(s)	690, 1113
ZrmI	AGTACT	1 cut(s)	724
Zsp2I	ATGCAT	1 cut(s)	971

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.