Rh5CG138800

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005207
Physical Location & Seq
Forward (+)
11665958 .. 11707232
41275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG138800.1

Sequence Viewer

Length: 765 bp
ATGGTCCAGATCATCGTGCTCCAGTGCATCATTCGGTACACTCTGTTACTCTTCCTGTTCGAGTACCGCAGCGCCAAGCTGCTGATAATGGAGCAGTTCCCGGAGACCGCCGCCTCCATCGTCTCCTTCAAGGTCGACTCCGAAGTCATGTCCCTCGACGGCCGCGACTTCCTTGAAAGGCAAGCTCCACGTCAAGGTGAGGAAGTCCAACGTGTCCAGAAGCTCTGCCAAGGCCCAGTTGACGCTGACATTCTGCTCCCCACTCAATTCGCCGCCTCCGACTCCTCGCAGAGGTGTATGACGCTTGACCCCAAGCTCAATTACCGCCCGGTGATTGGTATTCATAGCCATCCCAGCGACGGCGCCTCCGGACGGCTCAGTAATGTTGCTCGGAGGAGCATCTTGATTTGGAGGTTGTCGTCGCCATCGCTGTCGCCGACGAATCAGAAACGATTCGAGCTCTGTTTTCGGAATCCAAAAGAAAGAAGGAAAATCCAATTCCAAACGACTCCGATCGTCTTCTTGAGCATACAAGTGATGGCATCTACCAGCACAACGAAACGGGTCAAAAGATTGTTGGAAGGAGGATGTAAACCTAATCAATTTTATCAAGTTAATTCAAAGAGATGGAAAAAAATGTTTGAGGAACAAGTAGTTGAAGGATCGAATATAGTTGGAGATGAAGACGCGTTCGCAATGCCAGAAGGCATTGAAAATACTCCAAAGTTGCATATACTCAAGAACTTGGGAAAACCAGTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000578 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009888 GO:0009914 GO:0009921 GO:0009926 GO:0009942 GO:0009958 GO:0009966 GO:0009986 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0012506 GO:0015562 GO:0016020 GO:0016043 GO:0016049 GO:0016328 GO:0021700 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0030054 GO:0030154 GO:0031090 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048583 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0080161 GO:0090558 GO:0090627 GO:0098796 GO:0098797 GO:0099402 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

254

Amino Acids

28.95

Weight (kDa)

9.44

Isoelectric Point (pI)

50.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 1 - 145 6.6e-13 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 362
AccI GTMKAC 1 cut(s) 135
AccII CGCG 2 cut(s) 165, 689
AccIII TCCGGA 1 cut(s) 368
AciI CCGC 6 cut(s) 67, 108, 111, 163, 273, 325
AclWI GGATC 1 cut(s) 670
AcoI YGGCCR 1 cut(s) 160
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 2 cut(s) 38, 65
AfiI CCNNNNNNNGG 5 cut(s) 194, 291, 335, 359, 372
AflIII ACRYGT 2 cut(s) 211, 687
AgsI TTSAA 5 cut(s) 130, 176, 621, 659, 713
AjiI CACGTC 1 cut(s) 191
AluBI AGCT 5 cut(s) 79, 185, 223, 316, 460
AluI AGCT 5 cut(s) 79, 185, 223, 316, 460
Alw21I GWGCWC 2 cut(s) 21, 462
Alw26I GTCTC 2 cut(s) 98, 127
AlwI GGATC 1 cut(s) 670
Aor13HI TCCGGA 1 cut(s) 368
AoxI GGCC 2 cut(s) 160, 232
ApeKI GCWGC 2 cut(s) 69, 79
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 2 cut(s) 74, 365
AspS9I GGNCC 2 cut(s) 4, 233
AsuC2I CCSGG 2 cut(s) 101, 329
AsuHPI GGTGA 2 cut(s) 209, 343
AvaII GGWCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 462
BbsI GAAGAC 2 cut(s) 511, 690
Bbv12I GWGCWC 2 cut(s) 21, 462
BbvI GCAGC 2 cut(s) 66, 81
BccI CCATC 5 cut(s) 125, 357, 433, 532, 621
BceAI ACGGC 3 cut(s) 175, 376, 389
BcnI CCSGG 2 cut(s) 101, 329
BcoDI GTCTC 2 cut(s) 98, 127
BfoI RGCGCY 2 cut(s) 75, 366
BisI GCNGC 5 cut(s) 70, 80, 111, 163, 273
BlsI GCNGC 5 cut(s) 71, 81, 112, 164, 274
Bme1390I CCNGG 2 cut(s) 101, 329
Bme18I GGWCC 1 cut(s) 4
BmgBI CACGTC 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 4, 233
BmiI GGNNCC 1 cut(s) 364
BmrFI CCNGG 2 cut(s) 101, 329
BmrI ACTGGG 1 cut(s) 230
BmsI GCATC 3 cut(s) 36, 408, 551
BmuI ACTGGG 1 cut(s) 230
BpiI GAAGAC 2 cut(s) 511, 690
BpmI CTGGAG 1 cut(s) 5
BpuEI CTTGAG 2 cut(s) 544, 722
BpuMI CCSGG 2 cut(s) 101, 329
BsaHI GRCGYC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 1 cut(s) 229
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 5 cut(s) 194, 291, 335, 359, 372
Bse1I ACTGG 3 cut(s) 22, 236, 755
Bse3DI GCAATG 1 cut(s) 702
BseAI TCCGGA 1 cut(s) 368
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 2 cut(s) 349, 593
BseLI CCNNNNNNNGG 5 cut(s) 194, 291, 335, 359, 372
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 1 cut(s) 391
BseNI ACTGG 3 cut(s) 22, 236, 755
BseRI GAGGAG 2 cut(s) 274, 409
BseX3I CGGCCG 1 cut(s) 160
BseXI GCAGC 2 cut(s) 66, 81
BseYI CCCAGC 1 cut(s) 353
Bsh1236I CGCG 2 cut(s) 165, 689
Bsh1285I CGRYCG 2 cut(s) 163, 516
BshFI GGCC 2 cut(s) 162, 234
BshNI GGYRCC 1 cut(s) 362
BsiEI CGRYCG 2 cut(s) 163, 516
BsiHKAI GWGCWC 2 cut(s) 21, 462
BsiSI CCGG 3 cut(s) 101, 329, 369
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 5 cut(s) 194, 291, 335, 359, 372
BsmAI GTCTC 2 cut(s) 98, 127
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 136
BsnI GGCC 2 cut(s) 162, 234
Bso31I GGTCTC 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 21, 462
Bsp13I TCCGGA 1 cut(s) 368
Bsp143I GATC 3 cut(s) 9, 513, 662
BspACI CCGC 6 cut(s) 67, 108, 111, 163, 273, 325
BspANI GGCC 2 cut(s) 162, 234
BspCNI CTCAG 1 cut(s) 390
BspEI TCCGGA 1 cut(s) 368
BspFNI CGCG 2 cut(s) 165, 689
BspLI GGNNCC 1 cut(s) 364
BspPI GGATC 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 362
BspTNI GGTCTC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 702
BsrI ACTGG 3 cut(s) 22, 236, 755
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 3 cut(s) 9, 513, 662
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 229
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 363
BstC8I GCNNGC 1 cut(s) 183
BstDEI CTNAG 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 289
BstF5I GGATG 2 cut(s) 349, 593
BstFNI CGCG 2 cut(s) 165, 689
BstH2I RGCGCY 2 cut(s) 75, 366
BstHHI GCGC 2 cut(s) 74, 365
BstKTI GATC 3 cut(s) 12, 516, 665
BstMAI GTCTC 2 cut(s) 98, 127
BstMBI GATC 3 cut(s) 9, 513, 662
BstMCI CGRYCG 2 cut(s) 163, 516
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstSCI CCNGG 2 cut(s) 99, 327
BstUI CGCG 2 cut(s) 165, 689
BstV1I GCAGC 2 cut(s) 66, 81
BstV2I GAAGAC 2 cut(s) 511, 690
BstZI CGGCCG 1 cut(s) 160
BsuRI GGCC 2 cut(s) 162, 234
BtgZI GCGATG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 191
BtsCI GGATG 2 cut(s) 349, 593
BtsIMutI CAGTG 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 183
CfoI GCGC 2 cut(s) 74, 365
Cfr13I GGNCC 2 cut(s) 4, 233
CseI GACGC 3 cut(s) 251, 310, 695
Csp6I GTAC 2 cut(s) 37, 64
CviAII CATG 1 cut(s) 148
CviJI RGCY 9 cut(s) 79, 162, 185, 223, 234, 316, 348, 376, 460
CviKI_1 RGCY 9 cut(s) 79, 162, 185, 223, 234, 316, 348, 376, 460
CviQI GTAC 2 cut(s) 37, 64
DdeI CTNAG 1 cut(s) 377
DinI GGCGCC 1 cut(s) 364
DpnI GATC 3 cut(s) 11, 515, 664
DpnII GATC 3 cut(s) 9, 513, 662
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 160
EagI CGGCCG 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Ecl136II GAGCTC 1 cut(s) 460
EclXI CGGCCG 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 462
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 4
Eco52I CGGCCG 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 460
EcoNI CCTNNNNNAGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 462
EgeI GGCGCC 1 cut(s) 364
EheI GGCGCC 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 229
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 1 cut(s) 151
FaiI YATR 7 cut(s) 149, 299, 345, 530, 671, 732, 734
FaqI GGGAC 1 cut(s) 136
FatI CATG 1 cut(s) 147
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 70, 80, 111, 163, 273
FokI GGATG 2 cut(s) 336, 600
FriOI GRGCYC 1 cut(s) 462
Fsp4HI GCNGC 5 cut(s) 70, 80, 111, 163, 273
GlaI GCGC 2 cut(s) 73, 364
GluI GCNGC 5 cut(s) 70, 80, 111, 163, 273
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 5
HaeII RGCGCY 2 cut(s) 75, 366
HaeIII GGCC 2 cut(s) 162, 234
HapII CCGG 3 cut(s) 101, 329, 369
HgaI GACGC 3 cut(s) 251, 310, 695
HhaI GCGC 2 cut(s) 74, 365
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 2 cut(s) 72, 363
HinP1I GCGC 2 cut(s) 72, 363
HincII GTYRAC 2 cut(s) 136, 241
HindII GTYRAC 2 cut(s) 136, 241
HinfI GANTC 6 cut(s) 137, 281, 442, 453, 472, 508
HpaII CCGG 3 cut(s) 101, 329, 369
HphI GGTGA 2 cut(s) 209, 343
Hpy166II GTNNAC 4 cut(s) 39, 136, 241, 593
Hpy188I TCNGA 6 cut(s) 142, 280, 393, 447, 471, 513
Hpy188III TCNNGA 6 cut(s) 7, 217, 369, 403, 523, 739
Hpy8I GTNNAC 4 cut(s) 39, 136, 241, 593
Hpy99I CGWCG 4 cut(s) 161, 362, 424, 442
HpyAV CCTTC 5 cut(s) 136, 480, 575, 653, 698
HpyCH4IV ACGT 2 cut(s) 190, 211
HpyCH4V TGCA 2 cut(s) 27, 730
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 1 cut(s) 377
HpySE526I ACGT 2 cut(s) 190, 211
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 2 cut(s) 72, 363
KasI GGCGCC 1 cut(s) 362
Kpn2I TCCGGA 1 cut(s) 368
Kzo9I GATC 3 cut(s) 9, 513, 662
LmnI GCTCC 5 cut(s) 24, 91, 190, 261, 396
Lsp1109I GCAGC 2 cut(s) 66, 81
LweI GCATC 3 cut(s) 36, 408, 551
MaeII ACGT 2 cut(s) 190, 211
MaeIII GTNAC 1 cut(s) 45
MalI GATC 3 cut(s) 11, 515, 664
MboI GATC 3 cut(s) 9, 513, 662
MboII GAAGA 3 cut(s) 43, 511, 695
MhlI GDGCHC 2 cut(s) 21, 462
MluCI AATT 5 cut(s) 266, 319, 497, 602, 616
MluI ACGCGT 1 cut(s) 687
Mly113I GGCGCC 1 cut(s) 363
MlyI GAGTC 3 cut(s) 131, 275, 502
MmeI TCCRAC 4 cut(s) 232, 303, 558, 655
MroI TCCGGA 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 2 cut(s) 615, 763
MslI CAYNNNNRTG 1 cut(s) 533
MspI CCGG 3 cut(s) 101, 329, 369
MspR9I CCNGG 2 cut(s) 101, 329
MvnI CGCG 2 cut(s) 165, 689
MwoI GCNNNNNNNGC 1 cut(s) 354
NarI GGCGCC 1 cut(s) 363
NciI CCSGG 2 cut(s) 101, 329
NdeII GATC 3 cut(s) 9, 513, 662
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 276, 434
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 3 cut(s) 442, 453, 472
PfoI TCCNGGA 1 cut(s) 99
PkrI GCNGC 5 cut(s) 71, 81, 112, 164, 274
Ple19I CGATCG 1 cut(s) 516
PleI GAGTC 3 cut(s) 131, 275, 502
PluTI GGCGCC 1 cut(s) 366
PpsI GAGTC 3 cut(s) 131, 275, 502
Psp124BI GAGCTC 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 353
PspN4I GGNNCC 1 cut(s) 364
PspPI GGNCC 2 cut(s) 4, 233
PvuI CGATCG 1 cut(s) 516
RsaI GTAC 2 cut(s) 38, 65
RsaNI GTAC 2 cut(s) 37, 64
RseI CAYNNNNRTG 1 cut(s) 533
SacI GAGCTC 1 cut(s) 462
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 2 cut(s) 615, 763
SatI GCNGC 5 cut(s) 70, 80, 111, 163, 273
Sau3AI GATC 3 cut(s) 9, 513, 662
Sau96I GGNCC 2 cut(s) 4, 233
SchI GAGTC 3 cut(s) 131, 275, 502
ScrFI CCNGG 2 cut(s) 101, 329
SduI GDGCHC 2 cut(s) 21, 462
SfaNI GCATC 3 cut(s) 36, 408, 551
SfoI GGCGCC 1 cut(s) 364
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 533
SmlI CTYRAG 2 cut(s) 523, 737
SmoI CTYRAG 2 cut(s) 523, 737
Sse9I AATT 5 cut(s) 266, 319, 497, 602, 616
SsiI CCGC 6 cut(s) 67, 108, 111, 163, 273, 325
SspDI GGCGCC 1 cut(s) 362
SstI GAGCTC 1 cut(s) 462
StyD4I CCNGG 2 cut(s) 99, 327
StyI CCWWGG 1 cut(s) 229
TaiI ACGT 2 cut(s) 193, 214
TaqI TCGA 5 cut(s) 60, 135, 156, 456, 665
TasI AATT 5 cut(s) 266, 319, 497, 602, 616
TauI GCSGC 3 cut(s) 113, 165, 275
TfiI GAWTC 3 cut(s) 442, 453, 472
Tru1I TTAA 2 cut(s) 615, 763
Tru9I TTAA 2 cut(s) 615, 763
TscAI CASTG 1 cut(s) 29
TseI GCWGC 2 cut(s) 69, 79
TspDTI ATGAA 2 cut(s) 332, 696
TspRI CASTG 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 4
XagI CCTNNNNNAGG 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 135
XmnI GAANNNNTTC 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.