Rw4G026500

Iojap-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002364
Physical Location & Seq
Forward (+)
52711504 .. 52713873
2370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026500.1

Sequence Viewer

Length: 471 bp
ATGTTGGCCGCTCTACGCTCCCGATCGTCGTCCTCACTGCTTCTTCAGCGATGGAGGCTAGGGTTTCCATCTCTGCACCAGGCCTTCTCCGCCGCCGCAGCCAAGGAAGGCCTGCTCGATCTCCAGGAGGTCGAGACGGTTCTCAGCGACGTCAAGGCCGACGACGTCAAGGTCATACCGGCGAACAAGCACTGCGAGTGGGCCGATTTCATGGTCCTCGCCACCGGAAGGTCCACCTGGCACGTCAAGAACATCGCCCAAGCCCTAATTTACAAGTCTAAGCAAAAGCAGAAAGGAGCTCAGCGCTTGGTGCTTCCCACTGTTGAAGGGCAAGAGGGAGGAAAGTGGATTGTCATCGACTCTGGGAAAGTGATAGTTCATGCTCTTGATGAGAATGCTAGAGCTTACTACAATTTGGAGACTCTTTGGATAACAAAGCCATCTGAGAAAGAACCAATTCAGGGCATATAA

Protein Analysis

156

Amino Acids

17.36

Weight (kDa)

9.01

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RsfS PF02410 43 - 143 1.5e-25 Ribosomal silencing factor during starvation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
AatII GACGTC 2 cut(s) 153, 168
AccBSI CCGCTC 1 cut(s) 11
AciI CCGC 4 cut(s) 9, 90, 93, 96
AcoI YGGCCR 1 cut(s) 6
AcuI CTGAAG 1 cut(s) 29
AcyI GRCGYC 2 cut(s) 150, 165
AfeI AGCGCT 1 cut(s) 305
AfiI CCNNNNNNNGG 2 cut(s) 228, 461
AgsI TTSAA 1 cut(s) 326
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 3 cut(s) 78, 123, 236
AluBI AGCT 2 cut(s) 299, 404
AluI AGCT 2 cut(s) 299, 404
Alw21I GWGCWC 1 cut(s) 301
Alw26I GTCTC 2 cut(s) 128, 413
Aor51HI AGCGCT 1 cut(s) 305
AoxI GGCC 5 cut(s) 6, 81, 109, 156, 201
ApeKI GCWGC 1 cut(s) 98
Asp700I GAANNNNTTC 1 cut(s) 456
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 3 cut(s) 201, 214, 231
AvaII GGWCC 2 cut(s) 214, 231
BanII GRGCYC 1 cut(s) 301
Bbv12I GWGCWC 1 cut(s) 301
BbvI GCAGC 1 cut(s) 110
BccI CCATC 3 cut(s) 45, 76, 448
BciT130I CCWGG 3 cut(s) 80, 125, 238
BcoDI GTCTC 2 cut(s) 128, 413
BfaI CTAG 2 cut(s) 59, 399
BfoI RGCGCY 1 cut(s) 307
BisI GCNGC 4 cut(s) 9, 93, 96, 99
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 4 cut(s) 10, 94, 97, 100
Bme1390I CCNGG 3 cut(s) 80, 125, 238
Bme18I GGWCC 2 cut(s) 214, 231
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 3 cut(s) 201, 214, 231
BmrFI CCNGG 3 cut(s) 80, 125, 238
BpmI CTGGAG 1 cut(s) 107
Bpu1102I GCTNAGC 1 cut(s) 300
BsaBI GATNNNNATC 1 cut(s) 353
BsaHI GRCGYC 2 cut(s) 150, 165
BsaJI CCNNGG 1 cut(s) 102
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 461
Bse118I RCCGGY 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 353
BseBI CCWGG 3 cut(s) 80, 125, 238
BseDI CCNNGG 1 cut(s) 102
BseJI GATNNNNATC 1 cut(s) 353
BseLI CCNNNNNNNGG 2 cut(s) 228, 461
BseMII CTCAG 3 cut(s) 157, 314, 435
BseXI GCAGC 1 cut(s) 110
BsgI GTGCAG 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 5 cut(s) 8, 83, 111, 158, 203
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 301
BsiSI CCGG 2 cut(s) 179, 225
BslI CCNNNNNNNGG 2 cut(s) 228, 461
BsmAI GTCTC 2 cut(s) 128, 413
BsmBI CGTCTC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 5 cut(s) 8, 83, 111, 158, 203
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 2 cut(s) 23, 118
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 4 cut(s) 9, 90, 93, 96
BspANI GGCC 5 cut(s) 8, 83, 111, 158, 203
BspCNI CTCAG 3 cut(s) 156, 313, 436
BsrBI CCGCTC 1 cut(s) 11
BsrFI RCCGGY 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 2 cut(s) 23, 118
BssNI GRCGYC 2 cut(s) 150, 165
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 3 cut(s) 80, 125, 238
Bst4CI ACNGT 2 cut(s) 139, 322
BstACI GRCGYC 2 cut(s) 150, 165
BstC8I GCNNGC 1 cut(s) 113
BstDEI CTNAG 4 cut(s) 143, 279, 300, 444
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 2 cut(s) 26, 121
BstMAI GTCTC 2 cut(s) 128, 413
BstMBI GATC 2 cut(s) 23, 118
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 5 cut(s) 46, 55, 89, 98, 310
BstNI CCWGG 3 cut(s) 80, 125, 238
BstSCI CCNGG 3 cut(s) 78, 123, 236
BstV1I GCAGC 1 cut(s) 110
BsuRI GGCC 5 cut(s) 8, 83, 111, 158, 203
BtgZI GCGATG 2 cut(s) 64, 238
BtrI CACGTC 1 cut(s) 244
BtsI GCAGTG 2 cut(s) 35, 190
BtsIMutI CAGTG 3 cut(s) 35, 190, 318
Cac8I GCNNGC 1 cut(s) 113
CfoI GCGC 1 cut(s) 306
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 3 cut(s) 201, 214, 231
CviAII CATG 2 cut(s) 211, 380
DdeI CTNAG 4 cut(s) 143, 279, 300, 444
DpnI GATC 2 cut(s) 25, 120
DpnII GATC 2 cut(s) 23, 118
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
EaeI YGGCCR 1 cut(s) 6
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 102
Eco147I AGGCCT 2 cut(s) 83, 111
Eco24I GRGCYC 1 cut(s) 301
Eco47I GGWCC 2 cut(s) 214, 231
Eco47III AGCGCT 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 299
Eco57I CTGAAG 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 299
EcoRII CCWGG 3 cut(s) 78, 123, 236
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 102
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 2 cut(s) 214, 383
FaiI YATR 5 cut(s) 176, 212, 381, 467, 469
FatI CATG 2 cut(s) 210, 379
Fnu4HI GCNGC 4 cut(s) 9, 93, 96, 99
FriOI GRGCYC 1 cut(s) 301
Fsp4HI GCNGC 4 cut(s) 9, 93, 96, 99
FspBI CTAG 2 cut(s) 59, 399
GlaI GCGC 1 cut(s) 305
GluI GCNGC 4 cut(s) 9, 93, 96, 99
GsuI CTGGAG 1 cut(s) 107
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 5 cut(s) 8, 83, 111, 158, 203
HapII CCGG 2 cut(s) 179, 225
HhaI GCGC 1 cut(s) 306
Hin1I GRCGYC 2 cut(s) 150, 165
Hin1II CATG 2 cut(s) 214, 383
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HinfI GANTC 2 cut(s) 359, 421
HpaII CCGG 2 cut(s) 179, 225
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 1 cut(s) 445
Hpy188III TCNNGA 4 cut(s) 21, 133, 247, 386
Hpy8I GTNNAC 1 cut(s) 234
Hpy99I CGWCG 4 cut(s) 31, 152, 164, 167
HpyAV CCTTC 4 cut(s) 94, 101, 222, 320
HpyCH4III ACNGT 2 cut(s) 139, 322
HpyCH4IV ACGT 3 cut(s) 150, 165, 243
HpyCH4V TGCA 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 55, 89, 98, 310
HpyF3I CTNAG 4 cut(s) 143, 279, 300, 444
HpySE526I ACGT 3 cut(s) 150, 165, 243
Hsp92I GRCGYC 2 cut(s) 150, 165
Hsp92II CATG 2 cut(s) 214, 383
HspAI GCGC 1 cut(s) 304
Kzo9I GATC 2 cut(s) 23, 118
LmnI GCTCC 2 cut(s) 23, 296
Lsp1109I GCAGC 1 cut(s) 110
MaeI CTAG 2 cut(s) 59, 399
MaeII ACGT 3 cut(s) 150, 165, 243
MalI GATC 2 cut(s) 25, 120
MbiI CCGCTC 1 cut(s) 11
MboI GATC 2 cut(s) 23, 118
MboII GAAGA 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 3 cut(s) 267, 412, 456
MlyI GAGTC 2 cut(s) 353, 415
MnlI CCTC 6 cut(s) 43, 48, 121, 227, 328, 332
MroXI GAANNNNTTC 1 cut(s) 456
MspI CCGG 2 cut(s) 179, 225
MspR9I CCNGG 3 cut(s) 80, 125, 238
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 3 cut(s) 80, 125, 238
MwoI GCNNNNNNNGC 5 cut(s) 46, 55, 89, 98, 310
NdeII GATC 2 cut(s) 23, 118
NlaIII CATG 2 cut(s) 214, 383
PceI AGGCCT 2 cut(s) 83, 111
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 456
PflFI GACNNNGTC 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 123
PkrI GCNGC 4 cut(s) 10, 94, 97, 100
Ple19I CGATCG 1 cut(s) 26
PleI GAGTC 2 cut(s) 353, 415
PpsI GAGTC 2 cut(s) 353, 415
Psp124BI GAGCTC 1 cut(s) 301
Psp6I CCWGG 3 cut(s) 78, 123, 236
PspGI CCWGG 3 cut(s) 78, 123, 236
PspPI GGNCC 3 cut(s) 201, 214, 231
PsyI GACNNNGTC 1 cut(s) 164
PvuI CGATCG 1 cut(s) 26
SacI GAGCTC 1 cut(s) 301
SatI GCNGC 4 cut(s) 9, 93, 96, 99
Sau3AI GATC 2 cut(s) 23, 118
Sau96I GGNCC 3 cut(s) 201, 214, 231
SchI GAGTC 2 cut(s) 353, 415
ScrFI CCNGG 3 cut(s) 80, 125, 238
SduI GDGCHC 1 cut(s) 301
SetI ASST 9 cut(s) 132, 153, 168, 174, 233, 239, 246, 301, 406
SinI GGWCC 2 cut(s) 214, 231
Sse9I AATT 3 cut(s) 267, 412, 456
SseBI AGGCCT 2 cut(s) 83, 111
SsiI CCGC 4 cut(s) 9, 90, 93, 96
SspMI CTAG 2 cut(s) 59, 399
SstI GAGCTC 1 cut(s) 301
StuI AGGCCT 2 cut(s) 83, 111
StyD4I CCNGG 3 cut(s) 78, 123, 236
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 2 cut(s) 139, 322
TaiI ACGT 3 cut(s) 153, 168, 246
TaqI TCGA 3 cut(s) 117, 132, 357
TasI AATT 3 cut(s) 267, 412, 456
TauI GCSGC 3 cut(s) 11, 95, 98
TscAI CASTG 3 cut(s) 42, 197, 325
TseI GCWGC 1 cut(s) 98
TspDTI ATGAA 2 cut(s) 199, 368
TspRI CASTG 3 cut(s) 42, 197, 325
Tth111I GACNNNGTC 1 cut(s) 164
VpaK11BI GGWCC 2 cut(s) 214, 231
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 2 cut(s) 59, 399
ZraI GACGTC 2 cut(s) 151, 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.