AT1G14048

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004333
Physical Location & Seq
Reverse (-)
4815683 .. 4815988
306 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14048.1

Sequence Viewer

Length: 306 bp
ATGAAGGATTTCTTGCCGTCAACTGTTTGGCGAGATCCAGGGGAATCCGTATCCCCAAATGAAGTAAGGGAAGAAGAAGAGAAAGGAGAGGTGTTTTCAGCGTTCATGAGAGGAGGTGGTTGCAAGGAACCATTTACTGACTGGGAAGATTGCACTGATGAAGCTACCAACGTTGGAGTCTTTGCGATGATGACCAAATGTATGGTATGGATGCTCACTGATCACTATCGTCCGTTTCTTGCGGCCAAAAAAACTGCTCAAGAACACATAGAGAAGGAGCTTCAAGCCTTTCTCTCGAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

101

Amino Acids

11.62

Weight (kDa)

4.76

Isoelectric Point (pI)

58.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 30 - 93 1.2e-15 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 242
AclI AACGTT 1 cut(s) 171
AclWI GGATC 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 243
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 1 cut(s) 37
AluBI AGCT 2 cut(s) 164, 280
AluI AGCT 2 cut(s) 164, 280
AlwI GGATC 1 cut(s) 29
AoxI GGCC 1 cut(s) 243
Asp700I GAANNNNTTC 1 cut(s) 8
BciT130I CCWGG 1 cut(s) 39
BciVI GTATCC 1 cut(s) 61
BclI TGATCA 1 cut(s) 220
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 39
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 39
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 146
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 38
BseGI GGATG 1 cut(s) 216
BseJI GATNNNNATC 1 cut(s) 225
BseNI ACTGG 1 cut(s) 146
BseRI GAGGAG 1 cut(s) 126
BshFI GGCC 1 cut(s) 245
BsnI GGCC 1 cut(s) 245
Bsp143I GATC 2 cut(s) 34, 220
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 245
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 1 cut(s) 129
BspPI GGATC 1 cut(s) 29
BsrI ACTGG 1 cut(s) 146
BssECI CCNNGG 1 cut(s) 38
BssMI GATC 2 cut(s) 34, 220
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 72
BstF5I GGATG 1 cut(s) 216
BstKTI GATC 2 cut(s) 37, 223
BstMBI GATC 2 cut(s) 34, 220
BstNI CCWGG 1 cut(s) 39
BstSCI CCNGG 1 cut(s) 37
BstX2I RGATCY 1 cut(s) 34
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 34
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 1 cut(s) 245
BtgZI GCGATG 1 cut(s) 200
BtsCI GGATG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 153, 216
CciI TCATGA 1 cut(s) 105
CviAII CATG 1 cut(s) 106
CviJI RGCY 4 cut(s) 164, 245, 280, 287
CviKI_1 RGCY 4 cut(s) 164, 245, 280, 287
DpnI GATC 2 cut(s) 36, 222
DpnII GATC 2 cut(s) 34, 220
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 37
FaeI CATG 1 cut(s) 109
FaiI YATR 4 cut(s) 107, 203, 208, 269
FalI AAGNNNNNCTT 1 cut(s) 28
FatI CATG 1 cut(s) 105
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 243
GluI GCNGC 1 cut(s) 243
HaeIII GGCC 1 cut(s) 245
Hin1II CATG 1 cut(s) 109
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 2 cut(s) 44, 177
Hpy166II GTNNAC 1 cut(s) 21
Hpy188III TCNNGA 3 cut(s) 106, 260, 295
Hpy8I GTNNAC 1 cut(s) 21
HpyAV CCTTC 1 cut(s) 268
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 2 cut(s) 123, 153
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 2 cut(s) 34, 220
LmnI GCTCC 1 cut(s) 277
LpnPI CCDG 3 cut(s) 24, 51, 127
LweI GCATC 1 cut(s) 201
MaeII ACGT 1 cut(s) 171
MalI GATC 2 cut(s) 36, 222
MboI GATC 2 cut(s) 34, 220
MboII GAAGA 4 cut(s) 83, 86, 89, 158
MflI RGATCY 1 cut(s) 34
MlyI GAGTC 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 3 cut(s) 82, 104, 107
MroXI GAANNNNTTC 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 39
MvaI CCWGG 1 cut(s) 39
NdeII GATC 2 cut(s) 34, 220
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 1 cut(s) 129
PagI TCATGA 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 44
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
Psp1406I AACGTT 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 37
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 129
PsuI RGATCY 1 cut(s) 34
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 2 cut(s) 34, 220
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 1 cut(s) 39
SetI ASST 5 cut(s) 93, 118, 166, 174, 282
SfaNI GCATC 1 cut(s) 201
SmlI CTYRAG 1 cut(s) 258
SmoI CTYRAG 1 cut(s) 258
SsiI CCGC 1 cut(s) 242
StyD4I CCNGG 1 cut(s) 37
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 296
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 1 cut(s) 44
TscAI CASTG 2 cut(s) 160, 223
TspDTI ATGAA 4 cut(s) 17, 75, 94, 174
TspGWI ACGGA 2 cut(s) 37, 222
TspRI CASTG 2 cut(s) 160, 223
XcmI CCANNNNNNNNNTGG 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.