pycom06g00230

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004333
Physical Location & Seq
Reverse (-)
225694 .. 226215
522 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g00230.1

Sequence Viewer

Length: 522 bp
ATGATTCCCACTGATTCTCATAAGGCTGGCTTACCACCAAACTCATCAAATCCCCACATGGCCGATTCAGATTCTAAAACCCCAGACGACCAAAATCCAAAACATTCCTTAGACCCCCAATTGGATAACAAAACCCTAGAAGAAGAGAAGCGTGAGGAAGATGAAGGGCAATCAGAGAATGTTATGGAGGAAGAGGAGGAGGGAGAGTGTGGCTTTTGCTTGTTCATGAAAGGGGGAGGGTGCAAGGAGAGTTTCATAGCTTGGGAAGAGTGCGTTAGGGAGTCTGAGAAGACAAAGGAGGATGTTGTTGAAAAGTGCTTTGAGGTCACCGCAACATTGAAGAAGTGTATGCAGGCTCATTCAGATTACTATCTGCCCATTTTGAAGGCTGAGGAGGCAGCTGAGAAGGAGGTTGCCAAAGAATTGGAGAAAGAAAAGGATTCTGAGAGCTCGGAGAAAAATGCTACTCTGGAAAATACGGATCAAAAGGGTGGAAGTTCTAGTCAAAAAGGAGACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

174

Amino Acids

19.32

Weight (kDa)

4.5

Isoelectric Point (pI)

64.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 68 - 141 5.3e-35 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 330
AclWI GGATC 1 cut(s) 489
AcoI YGGCCR 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 121
AgsI TTSAA 3 cut(s) 311, 340, 385
AluBI AGCT 3 cut(s) 260, 401, 450
AluI AGCT 3 cut(s) 260, 401, 450
Alw21I GWGCWC 1 cut(s) 452
Alw26I GTCTC 1 cut(s) 507
AlwI GGATC 1 cut(s) 489
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 1 cut(s) 398
AsuHPI GGTGA 1 cut(s) 319
BanII GRGCYC 1 cut(s) 452
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 452
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 410
BcoDI GTCTC 1 cut(s) 507
BfaI CTAG 2 cut(s) 137, 501
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
BpiI GAAGAC 1 cut(s) 296
Bpu10I CCTNAGC 1 cut(s) 390
BsaBI GATNNNNATC 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse8I GATNNNNATC 1 cut(s) 369
BseGI GGATG 1 cut(s) 307
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMII CTCAG 4 cut(s) 276, 381, 393, 435
BseRI GAGGAG 3 cut(s) 209, 212, 407
BseXI GCAGC 1 cut(s) 410
BshFI GGCC 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 452
BslI CCNNNNNNNGG 1 cut(s) 121
BsmAI GTCTC 1 cut(s) 507
BsnI GGCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 452
Bsp143I GATC 1 cut(s) 481
BspACI CCGC 1 cut(s) 330
BspANI GGCC 1 cut(s) 62
BspCNI CTCAG 4 cut(s) 277, 382, 394, 436
BspHI TCATGA 1 cut(s) 225
BspPI GGATC 1 cut(s) 489
BssMI GATC 1 cut(s) 481
Bst6I CTCTTC 3 cut(s) 138, 186, 261
BstC8I GCNNGC 2 cut(s) 28, 354
BstDEI CTNAG 5 cut(s) 109, 285, 390, 402, 444
BstEII GGTNACC 1 cut(s) 325
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 1 cut(s) 484
BstMAI GTCTC 1 cut(s) 507
BstMBI GATC 1 cut(s) 481
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstPI GGTNACC 1 cut(s) 325
BstV1I GCAGC 1 cut(s) 410
BstV2I GAAGAC 1 cut(s) 296
BstXI CCANNNNNNTGG 1 cut(s) 424
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 1 cut(s) 307
BtsIMutI CAGTG 1 cut(s) 9
Cac8I GCNNGC 2 cut(s) 28, 354
CciI TCATGA 1 cut(s) 225
CviAII CATG 2 cut(s) 58, 226
CviJI RGCY 9 cut(s) 26, 30, 62, 213, 260, 356, 389, 401, 450
CviKI_1 RGCY 9 cut(s) 26, 30, 62, 213, 260, 356, 389, 401, 450
DdeI CTNAG 5 cut(s) 109, 285, 390, 402, 444
DpnI GATC 1 cut(s) 483
DpnII GATC 1 cut(s) 481
EaeI YGGCCR 1 cut(s) 60
Eam1104I CTCTTC 3 cut(s) 138, 186, 261
EarI CTCTTC 3 cut(s) 138, 186, 261
Ecl136II GAGCTC 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 452
Eco53kI GAGCTC 1 cut(s) 450
Eco91I GGTNACC 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 450
EcoO65I GGTNACC 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 452
FaeI CATG 2 cut(s) 61, 229
FaiI YATR 6 cut(s) 21, 59, 185, 227, 257, 350
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FatI CATG 2 cut(s) 57, 225
Fnu4HI GCNGC 1 cut(s) 399
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 1 cut(s) 452
Fsp4HI GCNGC 1 cut(s) 399
FspBI CTAG 2 cut(s) 137, 501
GluI GCNGC 1 cut(s) 399
HaeIII GGCC 1 cut(s) 62
Hin1II CATG 2 cut(s) 61, 229
HinfI GANTC 6 cut(s) 4, 14, 65, 71, 281, 440
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 6 cut(s) 70, 175, 286, 364, 445, 454
Hpy188III TCNNGA 2 cut(s) 226, 470
HpyAV CCTTC 3 cut(s) 158, 379, 400
HpyCH4V TGCA 2 cut(s) 243, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 5 cut(s) 109, 285, 390, 402, 444
Hsp92II CATG 2 cut(s) 61, 229
Kzo9I GATC 1 cut(s) 481
LpnPI CCDG 4 cut(s) 12, 96, 338, 455
Lsp1109I GCAGC 1 cut(s) 410
MaeI CTAG 2 cut(s) 137, 501
MaeIII GTNAC 1 cut(s) 325
MalI GATC 1 cut(s) 483
MboI GATC 1 cut(s) 481
MboII GAAGA 7 cut(s) 152, 155, 170, 203, 278, 301, 352
MfeI CAATTG 1 cut(s) 119
MhlI GDGCHC 1 cut(s) 452
MluCI AATT 3 cut(s) 119, 422, 517
MlyI GAGTC 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 401
MunI CAATTG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 395
NdeII GATC 1 cut(s) 481
NlaIII CATG 2 cut(s) 61, 229
NmuCI GTSAC 1 cut(s) 325
PagI TCATGA 1 cut(s) 225
PfeI GAWTC 5 cut(s) 4, 14, 65, 71, 440
PkrI GCNGC 1 cut(s) 400
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
Psp124BI GAGCTC 1 cut(s) 452
PspEI GGTNACC 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 401
SacI GAGCTC 1 cut(s) 452
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 1 cut(s) 481
SchI GAGTC 1 cut(s) 290
SduI GDGCHC 1 cut(s) 452
SetI ASST 5 cut(s) 262, 327, 403, 414, 452
Sse9I AATT 3 cut(s) 119, 422, 517
SsiI CCGC 1 cut(s) 330
SspMI CTAG 2 cut(s) 137, 501
SstI GAGCTC 1 cut(s) 452
TasI AATT 3 cut(s) 119, 422, 517
TfiI GAWTC 5 cut(s) 4, 14, 65, 71, 440
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 4 cut(s) 177, 214, 242, 244
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 1 cut(s) 16
XspI CTAG 2 cut(s) 137, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.