Rh1DG168800

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0004333
Physical Location & Seq
Forward (+)
34748654 .. 34749626
973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG168800.1

Sequence Viewer

Length: 498 bp
ATGTCCGATTCGGCTTCCAAACCCCAATCGGAAGACCAACCCCCGCCGCAGCCCCAATCGGACACAAAACCCTCCGAAGAAGAATCGGCGGCGGCGAAGCCCAAGGAAGACGATGCAGTCCAATCGGAGGAGGGGGAGGAGGAAGATGAGGAGAAGGGGGAGTGCGGGTTCTGCTTGTACATGAAAGGCGGCGGGTGCAAAGAGAGCTTTGTAGCTTGGGAAAACTGCATTGAAGAGGCGGAGAAGACGAACGAGGACATTGCTCAGAAGTGCTTCGACCTCACCTCCGCTCTCAGGACGTGTATGCTGGAACATTCCGATTACTACGAGCCCATTCTCAGGGCGGAGAAGGCCGCCGAGGAAGAAGCCATCAAGGAACTCGACAAGGAAAAGAAGGAAGAGAAAGAGAAGGAGAAGGAGAAGGAGGTGGTTATAGAATTGGAGAACGAAAAGGCTCCAGCTTCTGAGGTGGAGGAGAGTTCCGGTGGGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

165

Amino Acids

18.46

Weight (kDa)

4.32

Isoelectric Point (pI)

85.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 53 - 126 1.1e-34 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AccBSI CCGCTC 1 cut(s) 290
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 3 cut(s) 127, 294, 339
AflIII ACRYGT 1 cut(s) 299
AgsI TTSAA 1 cut(s) 233
AjiI CACGTC 1 cut(s) 300
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 3 cut(s) 207, 215, 461
AluI AGCT 3 cut(s) 207, 215, 461
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 1 cut(s) 49
Asp700I GAANNNNTTC 1 cut(s) 272
AsuHPI GGTGA 1 cut(s) 274
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 3 cut(s) 39, 114, 251
BbvI GCAGC 1 cut(s) 61
BccI CCATC 1 cut(s) 377
BcgI CGANNNNNNTGC 4 cut(s) 105, 139, 242, 276
BisI GCNGC 6 cut(s) 47, 50, 90, 93, 190, 354
BlsI GCNGC 6 cut(s) 48, 51, 91, 94, 191, 355
BmgBI CACGTC 1 cut(s) 300
BmiI GGNNCC 1 cut(s) 456
BmsI GCATC 1 cut(s) 103
BpiI GAAGAC 3 cut(s) 39, 114, 251
BpmI CTGGAG 1 cut(s) 441
BsaJI CCNNGG 2 cut(s) 102, 357
BsaWI WCCGGW 1 cut(s) 482
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 269, 299
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 294, 339
Bse3DI GCAATG 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 102, 357
BseLI CCNNNNNNNGG 3 cut(s) 127, 294, 339
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 4 cut(s) 278, 307, 352, 456
BseRI GAGGAG 4 cut(s) 143, 152, 164, 488
BseXI GCAGC 1 cut(s) 61
BshFI GGCC 1 cut(s) 353
BsiSI CCGG 1 cut(s) 483
BslI CCNNNNNNNGG 3 cut(s) 127, 294, 339
BsnI GGCC 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 333
Bsp1407I TGTACA 1 cut(s) 177
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 4 cut(s) 277, 306, 351, 457
BspLI GGNNCC 1 cut(s) 456
BsrBI CCGCTC 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 258
BsrGI TGTACA 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 102, 357
BssT1I CCWWGG 1 cut(s) 102
Bst6I CTCTTC 2 cut(s) 228, 393
BstAUI TGTACA 1 cut(s) 177
BstDEI CTNAG 4 cut(s) 264, 293, 338, 465
BstMWI GCNNNNNNNGC 4 cut(s) 171, 195, 204, 350
BstV1I GCAGC 1 cut(s) 61
BstV2I GAAGAC 3 cut(s) 39, 114, 251
BsuRI GGCC 1 cut(s) 353
BtrI CACGTC 1 cut(s) 300
CaiI CAGNNNCTG 1 cut(s) 464
Csp6I GTAC 1 cut(s) 178
CviAII CATG 1 cut(s) 181
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 4 cut(s) 264, 293, 338, 465
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
Eam1104I CTCTTC 2 cut(s) 228, 393
EarI CTCTTC 2 cut(s) 228, 393
EciI GGCGGA 2 cut(s) 254, 359
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 1 cut(s) 184
FaiI YATR 3 cut(s) 182, 305, 434
FatI CATG 1 cut(s) 180
FauI CCCGC 3 cut(s) 51, 158, 185
Fnu4HI GCNGC 6 cut(s) 47, 50, 90, 93, 190, 354
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 6 cut(s) 47, 50, 90, 93, 190, 354
GluI GCNGC 6 cut(s) 47, 50, 90, 93, 190, 354
GsuI CTGGAG 1 cut(s) 441
HaeIII GGCC 1 cut(s) 353
HapII CCGG 1 cut(s) 483
Hin1II CATG 1 cut(s) 184
HinfI GANTC 2 cut(s) 8, 83
HpaII CCGG 1 cut(s) 483
HphI GGTGA 1 cut(s) 274
Hpy188I TCNGA 8 cut(s) 7, 31, 61, 76, 127, 267, 319, 466
Hpy188III TCNNGA 1 cut(s) 295
HpyAV CCTTC 6 cut(s) 148, 343, 388, 403, 409, 415
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 3 cut(s) 116, 198, 228
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 195, 204, 350
HpyF3I CTNAG 4 cut(s) 264, 293, 338, 465
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 1 cut(s) 184
LmnI GCTCC 1 cut(s) 460
LpnPI CCDG 4 cut(s) 280, 293, 325, 471
Lsp1109I GCAGC 1 cut(s) 61
LweI GCATC 1 cut(s) 103
MaeII ACGT 1 cut(s) 299
MbiI CCGCTC 1 cut(s) 290
MboII GAAGA 9 cut(s) 44, 89, 92, 119, 155, 245, 256, 374, 410
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 1 cut(s) 437
MroXI GAANNNNTTC 1 cut(s) 272
MspI CCGG 1 cut(s) 483
MwoI GCNNNNNNNGC 4 cut(s) 171, 195, 204, 350
NlaIII CATG 1 cut(s) 184
NlaIV GGNNCC 1 cut(s) 456
NmeAIII GCCGAG 1 cut(s) 382
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 2 cut(s) 8, 83
PkrI GCNGC 6 cut(s) 48, 51, 91, 94, 191, 355
PspN4I GGNNCC 1 cut(s) 456
PstNI CAGNNNCTG 1 cut(s) 464
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SatI GCNGC 6 cut(s) 47, 50, 90, 93, 190, 354
SduI GDGCHC 1 cut(s) 333
SetI ASST 8 cut(s) 209, 217, 282, 287, 302, 429, 463, 471
SfaNI GCATC 1 cut(s) 103
Sse9I AATT 1 cut(s) 437
StyI CCWWGG 1 cut(s) 102
TaiI ACGT 1 cut(s) 302
TaqI TCGA 2 cut(s) 276, 381
TasI AATT 1 cut(s) 437
TatI WGTACW 1 cut(s) 177
TauI GCSGC 5 cut(s) 49, 92, 95, 192, 356
TfiI GAWTC 2 cut(s) 8, 83
TseI GCWGC 1 cut(s) 49
TspDTI ATGAA 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.