AT1G14060

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004333
Physical Location & Seq
Reverse (-)
4816928 .. 4818112
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14060.1

Sequence Viewer

Length: 540 bp
ATGGGAATTACATCATCCACAGATCCAAAAACAGAGACTTTTTCCGACCCCAAATCTCAAAACAACGAAGAAGCTCCTCCAGCTAAATCTGAGAAACTCGGAGATGCGTCTCCGTCGACTCCAGCTCCATCTGAGGAACTCGGAGATTCATCAACTGTTTGGAGAGACCCAGAAGATTCCGAATCGGATGAAAAACCAGAAGAAGGAGGAGGAGGAGACGCAGATGGTGAAGAGGAAGGAGAGTGTGGGTTTTGCTTGTTTATGAAAGGAGGTGGTTGCAAAGATTCGTTTGTTGCTTGGGAAGAGTGTGTTGAAGCAGCTGAGAAGAACAAGGAAGATATTGTTACTAATTGTATGGAAGTCACTTCGATGTTGAAGAAGTGTATGGATGAACATTCCGATTACTATCAGCCCATTCTCGCTGCGGAGAAAGCTGCTGAAGCAGAGGTTAAGAAGGAGCTTGAAGCTGAGAAGAAGAAGGAAGAAGAAGAGAAGATCTCGGAAGAGGAGGTAGCTGCGAAGAAGCAAGCTCAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

179

Amino Acids

19.43

Weight (kDa)

4.27

Isoelectric Point (pI)

68.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 80 - 153 1.7e-37 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 116
AciI CCGC 1 cut(s) 425
AclWI GGATC 1 cut(s) 17
AcuI CTGAAG 1 cut(s) 459
AfiI CCNNNNNNNGG 1 cut(s) 203
AgsI TTSAA 3 cut(s) 314, 376, 464
AluBI AGCT 9 cut(s) 74, 83, 125, 320, 434, 460, 467, 515, 530
AluI AGCT 9 cut(s) 74, 83, 125, 320, 434, 460, 467, 515, 530
Alw26I GTCTC 4 cut(s) 29, 114, 159, 210
AlwI GGATC 1 cut(s) 17
ApeKI GCWGC 4 cut(s) 317, 422, 434, 515
AsuHPI GGTGA 1 cut(s) 239
BbvI GCAGC 4 cut(s) 329, 409, 421, 502
BccI CCATC 2 cut(s) 136, 218
BcoDI GTCTC 4 cut(s) 29, 114, 159, 210
BglII AGATCT 1 cut(s) 495
BisI GCNGC 4 cut(s) 318, 423, 435, 516
BlsI GCNGC 4 cut(s) 319, 424, 436, 517
BmsI GCATC 1 cut(s) 94
BplI GAGNNNNNCTC 2 cut(s) 482, 514
BpmI CTGGAG 2 cut(s) 63, 105
BpuEI CTTGAG 1 cut(s) 516
BsaBI GATNNNNATC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse8I GATNNNNATC 1 cut(s) 405
BseGI GGATG 3 cut(s) 14, 193, 394
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMII CTCAG 4 cut(s) 81, 123, 312, 459
BseRI GAGGAG 5 cut(s) 66, 222, 225, 228, 521
BseXI GCAGC 4 cut(s) 329, 409, 421, 502
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 4 cut(s) 29, 114, 159, 210
BsmBI CGTCTC 2 cut(s) 114, 210
Bso31I GGTCTC 1 cut(s) 159
Bsp143I GATC 2 cut(s) 22, 495
BspACI CCGC 1 cut(s) 425
BspCNI CTCAG 4 cut(s) 82, 124, 313, 460
BspPI GGATC 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 159
BssMI GATC 2 cut(s) 22, 495
Bst4CI ACNGT 1 cut(s) 157
Bst6I CTCTTC 4 cut(s) 225, 297, 483, 498
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 4 cut(s) 90, 132, 321, 468
BstF5I GGATG 3 cut(s) 14, 193, 394
BstKTI GATC 2 cut(s) 25, 498
BstMAI GTCTC 4 cut(s) 29, 114, 159, 210
BstMBI GATC 2 cut(s) 22, 495
BstMWI GCNNNNNNNGC 3 cut(s) 80, 431, 440
BstV1I GCAGC 4 cut(s) 329, 409, 421, 502
BstX2I RGATCY 2 cut(s) 22, 495
BstYI RGATCY 2 cut(s) 22, 495
BtsCI GGATG 3 cut(s) 14, 193, 394
Cac8I GCNNGC 1 cut(s) 528
CseI GACGC 2 cut(s) 96, 227
DdeI CTNAG 4 cut(s) 90, 132, 321, 468
DpnI GATC 2 cut(s) 24, 497
DpnII GATC 2 cut(s) 22, 495
Eam1104I CTCTTC 4 cut(s) 225, 297, 483, 498
EarI CTCTTC 4 cut(s) 225, 297, 483, 498
Eco31I GGTCTC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 459
Esp3I CGTCTC 2 cut(s) 114, 210
FaiI YATR 3 cut(s) 263, 356, 386
FblI GTMKAC 1 cut(s) 116
Fnu4HI GCNGC 4 cut(s) 318, 423, 435, 516
FokI GGATG 2 cut(s) 200, 401
Fsp4HI GCNGC 4 cut(s) 318, 423, 435, 516
GluI GCNGC 4 cut(s) 318, 423, 435, 516
GsuI CTGGAG 2 cut(s) 63, 105
HgaI GACGC 2 cut(s) 96, 227
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HinfI GANTC 5 cut(s) 118, 146, 176, 182, 284
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 9 cut(s) 46, 91, 101, 133, 143, 181, 187, 400, 502
Hpy8I GTNNAC 1 cut(s) 117
Hpy99I CGWCG 1 cut(s) 118
HpyAV CCTTC 4 cut(s) 197, 230, 448, 472
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4V TGCA 1 cut(s) 279
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 431, 440
HpyF3I CTNAG 4 cut(s) 90, 132, 321, 468
Kzo9I GATC 2 cut(s) 22, 495
LmnI GCTCC 3 cut(s) 79, 130, 457
LpnPI CCDG 4 cut(s) 93, 135, 183, 210
Lsp1109I GCAGC 4 cut(s) 329, 409, 421, 502
LweI GCATC 1 cut(s) 94
MaeIII GTNAC 2 cut(s) 343, 361
MalI GATC 2 cut(s) 24, 497
MboI GATC 2 cut(s) 22, 495
MflI RGATCY 2 cut(s) 22, 495
MluCI AATT 2 cut(s) 6, 349
MlyI GAGTC 1 cut(s) 112
MmeI TCCRAC 1 cut(s) 69
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 320
MwoI GCNNNNNNNGC 3 cut(s) 80, 431, 440
NdeII GATC 2 cut(s) 22, 495
NmuCI GTSAC 1 cut(s) 361
PfeI GAWTC 4 cut(s) 146, 176, 182, 284
PkrI GCNGC 4 cut(s) 319, 424, 436, 517
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PsuI RGATCY 2 cut(s) 22, 495
PvuII CAGCTG 1 cut(s) 320
RseI CAYNNNNRTG 1 cut(s) 368
SalI GTCGAC 1 cut(s) 115
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 4 cut(s) 318, 423, 435, 516
Sau3AI GATC 2 cut(s) 22, 495
SchI GAGTC 1 cut(s) 112
SfaNI GCATC 1 cut(s) 94
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 531
SmoI CTYRAG 1 cut(s) 531
Sse9I AATT 2 cut(s) 6, 349
SsiI CCGC 1 cut(s) 425
TaaI ACNGT 1 cut(s) 157
TaqI TCGA 2 cut(s) 116, 368
TasI AATT 2 cut(s) 6, 349
TfiI GAWTC 4 cut(s) 146, 176, 182, 284
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 4 cut(s) 317, 422, 434, 515
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 4 cut(s) 138, 204, 278, 405
TspGWI ACGGA 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.