FvH4_7g07760

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004333
Physical Location & Seq
Forward (+)
7665610 .. 7666167
558 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07760.t1

Sequence Viewer

Length: 558 bp
ATGGGTGCTTCCAACTCCATTAACAGACCAGACTCAGCGGCCACCTCTCCCCCAAAGCTGGATTCAATTCCGTCCGACTCCAACTCCAACTCCATTCCCATGTCCGATTCAGCTACCAAAACCCAGCCGGAAGAGCCCCAACCGGTTACAAAACCCTCATCGGAAGCGGCGGCGGAGAAGCCCGATGCGGCCGAACCGGAGATCGGAGAGGAGGAGGAAGAGGAAGAGGAGAAAGGGGAGTGCGGGTTCTGCTTGTTCATGAAAGGCGGCGGGTGCAAAGAGAGCTTCGTGGCGTGGGAGAATTGCATAGAGGAGGCGGAGAAGTCGAACGAGGACATTGCTCAGAAGTGCTTCGACCTCACCTCCGCTCTCCGGGAGTGCATGGTGGCCCACCCAGATTACTACGAGCCCATTCTCCGCGCCGAGAAGGCCGCCGAGGAGGAGGCCATCAAGGAGCTCGACAAGGAGAAAGAGAATGAGGCGCTTATTGAAGTGGGGAATGAAAAGGAGGTGGCGTCAGAGAGCACAAAAGCAGCTTCGTCTGAGGGGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

186

Amino Acids

20.02

Weight (kDa)

4.28

Isoelectric Point (pI)

70.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 79 - 152 6.3e-35 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 368
AccII CGCG 1 cut(s) 420
AcoI YGGCCR 2 cut(s) 39, 189
AcyI GRCGYC 1 cut(s) 515
AfiI CCNNNNNNNGG 3 cut(s) 58, 203, 372
AgeI ACCGGT 1 cut(s) 142
AgsI TTSAA 2 cut(s) 66, 491
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
AluBI AGCT 5 cut(s) 58, 113, 285, 457, 536
AluI AGCT 5 cut(s) 58, 113, 285, 457, 536
Alw21I GWGCWC 2 cut(s) 459, 527
AoxI GGCC 5 cut(s) 39, 189, 387, 429, 444
ApeKI GCWGC 1 cut(s) 533
AsiGI ACCGGT 1 cut(s) 142
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 2 cut(s) 422, 484
AspS9I GGNCC 1 cut(s) 388
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 1 cut(s) 352
BanII GRGCYC 3 cut(s) 138, 411, 459
Bbv12I GWGCWC 2 cut(s) 459, 527
BbvI GCAGC 1 cut(s) 545
BccI CCATC 1 cut(s) 455
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BcnI CCSGG 1 cut(s) 374
BfoI RGCGCY 1 cut(s) 485
BglI GCCNNNNNGGC 1 cut(s) 428
BisI GCNGC 7 cut(s) 39, 168, 171, 189, 268, 432, 534
BlsI GCNGC 7 cut(s) 40, 169, 172, 190, 269, 433, 535
Bme1390I CCNGG 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 388
BmrFI CCNGG 1 cut(s) 374
BmsI GCATC 1 cut(s) 175
BpuMI CCSGG 1 cut(s) 374
BsaHI GRCGYC 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 435
BsaWI WCCGGW 2 cut(s) 142, 196
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 203, 372
Bse118I RCCGGY 1 cut(s) 142
Bse3DI GCAATG 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 435
BseLI CCNNNNNNNGG 3 cut(s) 58, 203, 372
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 3 cut(s) 48, 356, 534
BseRI GAGGAG 6 cut(s) 224, 227, 242, 326, 452, 455
BseX3I CGGCCG 1 cut(s) 189
BseXI GCAGC 1 cut(s) 545
BseYI CCCAGC 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 420
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 5 cut(s) 41, 191, 389, 431, 446
BshTI ACCGGT 1 cut(s) 142
BsiEI CGRYCG 1 cut(s) 192
BsiHKAI GWGCWC 2 cut(s) 459, 527
BsiSI CCGG 4 cut(s) 128, 143, 197, 373
BslI CCNNNNNNNGG 3 cut(s) 58, 203, 372
BsnI GGCC 5 cut(s) 41, 191, 389, 431, 446
Bsp1286I GDGCHC 4 cut(s) 138, 411, 459, 527
Bsp143I GATC 1 cut(s) 201
BspANI GGCC 5 cut(s) 41, 191, 389, 431, 446
BspCNI CTCAG 3 cut(s) 47, 355, 535
BspFNI CGCG 1 cut(s) 420
BspHI TCATGA 1 cut(s) 258
BspQI GCTCTTC 1 cut(s) 126
BsrBI CCGCTC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 336
BsrFI RCCGGY 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 1 cut(s) 201
BssNI GRCGYC 1 cut(s) 515
Bst6I CTCTTC 3 cut(s) 126, 213, 219
BstACI GRCGYC 1 cut(s) 515
BstDEI CTNAG 3 cut(s) 34, 342, 543
BstFNI CGCG 1 cut(s) 420
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 2 cut(s) 422, 484
BstKTI GATC 1 cut(s) 204
BstMBI GATC 1 cut(s) 201
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 5 cut(s) 133, 249, 273, 282, 428
BstSCI CCNGG 1 cut(s) 372
BstUI CGCG 1 cut(s) 420
BstV1I GCAGC 1 cut(s) 545
BstZI CGGCCG 1 cut(s) 189
BsuRI GGCC 5 cut(s) 41, 191, 389, 431, 446
CciI TCATGA 1 cut(s) 258
CfoI GCGC 2 cut(s) 422, 484
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 388
CseI GACGC 1 cut(s) 504
CspAI ACCGGT 1 cut(s) 142
CviAII CATG 3 cut(s) 100, 259, 382
DdeI CTNAG 3 cut(s) 34, 342, 543
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
EaeI YGGCCR 2 cut(s) 39, 189
EagI CGGCCG 1 cut(s) 189
Eam1104I CTCTTC 3 cut(s) 126, 213, 219
EarI CTCTTC 3 cut(s) 126, 213, 219
EciI GGCGGA 2 cut(s) 188, 332
Ecl136II GAGCTC 1 cut(s) 457
EclXI CGGCCG 1 cut(s) 189
Eco24I GRGCYC 3 cut(s) 138, 411, 459
Eco52I CGGCCG 1 cut(s) 189
Eco53kI GAGCTC 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 457
EcoT38I GRGCYC 3 cut(s) 138, 411, 459
FaeI CATG 3 cut(s) 103, 262, 385
FaiI YATR 4 cut(s) 101, 260, 308, 383
FatI CATG 3 cut(s) 99, 258, 381
FauI CCCGC 2 cut(s) 236, 263
Fnu4HI GCNGC 7 cut(s) 39, 168, 171, 189, 268, 432, 534
FriOI GRGCYC 3 cut(s) 138, 411, 459
Fsp4HI GCNGC 7 cut(s) 39, 168, 171, 189, 268, 432, 534
GlaI GCGC 2 cut(s) 421, 483
GluI GCNGC 7 cut(s) 39, 168, 171, 189, 268, 432, 534
GsaI CCCAGC 1 cut(s) 127
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 5 cut(s) 41, 191, 389, 431, 446
HapII CCGG 4 cut(s) 128, 143, 197, 373
HgaI GACGC 1 cut(s) 504
HhaI GCGC 2 cut(s) 422, 484
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 3 cut(s) 103, 262, 385
Hin6I GCGC 2 cut(s) 420, 482
HinP1I GCGC 2 cut(s) 420, 482
HinfI GANTC 4 cut(s) 32, 62, 77, 107
HpaII CCGG 4 cut(s) 128, 143, 197, 373
HphI GGTGA 1 cut(s) 352
Hpy188I TCNGA 7 cut(s) 76, 106, 163, 206, 345, 520, 544
Hpy188III TCNNGA 1 cut(s) 259
HpyAV CCTTC 1 cut(s) 421
HpyCH4V TGCA 3 cut(s) 276, 306, 381
HpyF10VI GCNNNNNNNGC 5 cut(s) 133, 249, 273, 282, 428
HpyF3I CTNAG 3 cut(s) 34, 342, 543
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 3 cut(s) 103, 262, 385
HspAI GCGC 2 cut(s) 420, 482
Kzo9I GATC 1 cut(s) 201
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 454
LpnPI CCDG 8 cut(s) 42, 44, 137, 141, 156, 210, 386, 408
Lsp1109I GCAGC 1 cut(s) 545
LweI GCATC 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 145
MalI GATC 1 cut(s) 203
MbiI CCGCTC 1 cut(s) 368
MboI GATC 1 cut(s) 201
MboII GAAGA 3 cut(s) 143, 230, 236
MhlI GDGCHC 4 cut(s) 138, 411, 459, 527
MluCI AATT 2 cut(s) 66, 301
MlyI GAGTC 2 cut(s) 26, 71
MmeI TCCRAC 4 cut(s) 36, 99, 105, 111
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 4 cut(s) 128, 143, 197, 373
MspR9I CCNGG 1 cut(s) 374
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 5 cut(s) 133, 249, 273, 282, 428
NciI CCSGG 1 cut(s) 374
NdeII GATC 1 cut(s) 201
NlaIII CATG 3 cut(s) 103, 262, 385
NmeAIII GCCGAG 2 cut(s) 448, 460
PagI TCATGA 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 2 cut(s) 62, 107
PfoI TCCNGGA 1 cut(s) 372
PinAI ACCGGT 1 cut(s) 142
PkrI GCNGC 7 cut(s) 40, 169, 172, 190, 269, 433, 535
PleI GAGTC 2 cut(s) 26, 71
PpsI GAGTC 2 cut(s) 26, 71
Psp124BI GAGCTC 1 cut(s) 459
PspFI CCCAGC 1 cut(s) 123
PspPI GGNCC 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 98
SacI GAGCTC 1 cut(s) 459
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 7 cut(s) 39, 168, 171, 189, 268, 432, 534
Sau3AI GATC 1 cut(s) 201
Sau96I GGNCC 1 cut(s) 388
SchI GAGTC 2 cut(s) 26, 71
ScrFI CCNGG 1 cut(s) 374
SduI GDGCHC 4 cut(s) 138, 411, 459, 527
SetI ASST 9 cut(s) 47, 60, 115, 287, 360, 365, 459, 513, 538
SfaNI GCATC 1 cut(s) 175
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 2 cut(s) 66, 301
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 1 cut(s) 372
TaqI TCGA 3 cut(s) 326, 354, 459
TasI AATT 2 cut(s) 66, 301
TauI GCSGC 6 cut(s) 41, 170, 173, 191, 270, 434
TfiI GAWTC 2 cut(s) 62, 107
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseI GCWGC 1 cut(s) 533
TspDTI ATGAA 3 cut(s) 247, 275, 516
TspGWI ACGGA 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.