Rroxscaffold_4G00312730

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004333
Physical Location & Seq
Reverse (-)
35872807 .. 35874124
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312730.1

Sequence Viewer

Length: 594 bp
ATGGGTGCTTCCAACTCCATTGACAGACCAGCAGCACCAGACTCATCAGAGGCGTCTCCCCCTCCAAAGATCGATTCAATTCCCTCCTCCGTCTCCATCTCCATGTCCGATTCGGCTTCCAAACCCCAATCGGAAGACCAACCCCCGCCGCAGCCCCAATCGGACACAAAACCCTCCGAAGAAGAATCGGCGGCGGCGAAGCCCAAGGAAGACGATGCAGTCCAATCGGAGGAGGAGGAGAATGAGGAAGATGAGGAGGAGAAGGGGGAGTGCGGGTTCTGCTTGTACATGAAAGGCGGCGGGTGCAAAGAGAGCTTTATAGCTTGGGAAAACTGCATTGAAGAGGCGGAGAAGACGAACGAGGACATTGCTCAGAAGTGCTTCGACCTCACCTCCGCTCTCAGGACGTGTATGCTGGAACATTCCGATTACTACGAGCCCATTCTCAGGGCGGAGAAGGCCGCCGAGGAAGAAGCCATCAAGGAACTCGACAAGGAAAAGAAGGAAGAGAAGGAGAAGGAGGTGGTTATAGAATTGGAGAACGAAGAGGCTCCAGCTTCTAAGGTGGAGGAGAGTTCCGGTGGGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

197

Amino Acids

21.63

Weight (kDa)

4.24

Isoelectric Point (pI)

88.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 89 - 162 9.2e-35 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AccBSI CCGCTC 1 cut(s) 398
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 3 cut(s) 229, 402, 447
AflIII ACRYGT 1 cut(s) 407
AgsI TTSAA 2 cut(s) 78, 341
AjiI CACGTC 1 cut(s) 408
AjuI GAANNNNNNNTTGG 2 cut(s) 58, 90
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 3 cut(s) 315, 323, 557
AluI AGCT 3 cut(s) 315, 323, 557
Alw26I GTCTC 2 cut(s) 60, 97
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 2 cut(s) 32, 151
Asp700I GAANNNNTTC 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 382
BanII GRGCYC 1 cut(s) 441
BbsI GAAGAC 3 cut(s) 141, 216, 359
BbvI GCAGC 2 cut(s) 44, 163
BccI CCATC 2 cut(s) 104, 485
BcgI CGANNNNNNTGC 4 cut(s) 207, 241, 350, 384
BcoDI GTCTC 2 cut(s) 60, 97
BisI GCNGC 7 cut(s) 33, 149, 152, 192, 195, 298, 462
BlsI GCNGC 7 cut(s) 34, 150, 153, 193, 196, 299, 463
BmgBI CACGTC 1 cut(s) 408
BmiI GGNNCC 1 cut(s) 552
BmsI GCATC 1 cut(s) 205
BpiI GAAGAC 3 cut(s) 141, 216, 359
BpmI CTGGAG 1 cut(s) 537
Bsa29I ATCGAT 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 204, 465
BsaWI WCCGGW 1 cut(s) 578
BsaXI ACNNNNNCTCC 4 cut(s) 260, 290, 377, 407
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 402, 447
Bse3DI GCAATG 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 204, 465
BseLI CCNNNNNNNGG 3 cut(s) 229, 402, 447
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 3 cut(s) 386, 415, 460
BseRI GAGGAG 7 cut(s) 76, 245, 248, 251, 269, 272, 584
BseXI GCAGC 2 cut(s) 44, 163
BshFI GGCC 1 cut(s) 461
BshVI ATCGAT 1 cut(s) 72
BsiSI CCGG 1 cut(s) 579
BslI CCNNNNNNNGG 3 cut(s) 229, 402, 447
BsmAI GTCTC 2 cut(s) 60, 97
BsmBI CGTCTC 2 cut(s) 60, 97
BsnI GGCC 1 cut(s) 461
Bsp1286I GDGCHC 1 cut(s) 441
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 1 cut(s) 69
BspANI GGCC 1 cut(s) 461
BspCNI CTCAG 3 cut(s) 385, 414, 459
BspDI ATCGAT 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 552
BsrBI CCGCTC 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 366
BsrGI TGTACA 1 cut(s) 285
BssECI CCNNGG 2 cut(s) 204, 465
BssMI GATC 1 cut(s) 69
BssNI GRCGYC 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 204
Bst6I CTCTTC 3 cut(s) 336, 501, 540
BstACI GRCGYC 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 285
BstDEI CTNAG 4 cut(s) 372, 401, 446, 561
BstKTI GATC 1 cut(s) 72
BstMAI GTCTC 2 cut(s) 60, 97
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 4 cut(s) 279, 303, 312, 458
BstV1I GCAGC 2 cut(s) 44, 163
BstV2I GAAGAC 3 cut(s) 141, 216, 359
Bsu15I ATCGAT 1 cut(s) 72
BsuRI GGCC 1 cut(s) 461
BsuTUI ATCGAT 1 cut(s) 72
BtrI CACGTC 1 cut(s) 408
ClaI ATCGAT 1 cut(s) 72
CseI GACGC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 286
CviAII CATG 2 cut(s) 103, 289
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 4 cut(s) 372, 401, 446, 561
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 3 cut(s) 336, 501, 540
EarI CTCTTC 3 cut(s) 336, 501, 540
EciI GGCGGA 2 cut(s) 362, 467
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 1 cut(s) 441
EcoT14I CCWWGG 1 cut(s) 204
EcoT38I GRGCYC 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 204
Esp3I CGTCTC 2 cut(s) 60, 97
FaeI CATG 2 cut(s) 106, 292
FaiI YATR 5 cut(s) 104, 290, 320, 413, 530
FatI CATG 2 cut(s) 102, 288
FauI CCCGC 3 cut(s) 153, 266, 293
Fnu4HI GCNGC 7 cut(s) 33, 149, 152, 192, 195, 298, 462
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 7 cut(s) 33, 149, 152, 192, 195, 298, 462
GluI GCNGC 7 cut(s) 33, 149, 152, 192, 195, 298, 462
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 1 cut(s) 461
HapII CCGG 1 cut(s) 579
HgaI GACGC 1 cut(s) 42
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 2 cut(s) 106, 292
HinfI GANTC 4 cut(s) 41, 74, 110, 185
HpaII CCGG 1 cut(s) 579
HphI GGTGA 1 cut(s) 382
Hpy188I TCNGA 8 cut(s) 49, 109, 133, 163, 178, 229, 375, 427
Hpy188III TCNNGA 1 cut(s) 403
HpyAV CCTTC 5 cut(s) 256, 451, 496, 505, 511
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 3 cut(s) 218, 306, 336
HpyF10VI GCNNNNNNNGC 4 cut(s) 279, 303, 312, 458
HpyF3I CTNAG 4 cut(s) 372, 401, 446, 561
HpySE526I ACGT 1 cut(s) 407
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 2 cut(s) 106, 292
Kzo9I GATC 1 cut(s) 69
LmnI GCTCC 1 cut(s) 556
LpnPI CCDG 6 cut(s) 42, 51, 388, 401, 433, 567
Lsp1109I GCAGC 2 cut(s) 44, 163
LweI GCATC 1 cut(s) 205
MaeII ACGT 1 cut(s) 407
MalI GATC 1 cut(s) 71
MbiI CCGCTC 1 cut(s) 398
MboI GATC 1 cut(s) 69
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 2 cut(s) 78, 533
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 380
MslI CAYNNNNRTG 1 cut(s) 101
MspI CCGG 1 cut(s) 579
MwoI GCNNNNNNNGC 4 cut(s) 279, 303, 312, 458
NdeII GATC 1 cut(s) 69
NlaIII CATG 2 cut(s) 106, 292
NlaIV GGNNCC 1 cut(s) 552
NmeAIII GCCGAG 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 3 cut(s) 74, 110, 185
PkrI GCNGC 7 cut(s) 34, 150, 153, 193, 196, 299, 463
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 552
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
RseI CAYNNNNRTG 1 cut(s) 101
SatI GCNGC 7 cut(s) 33, 149, 152, 192, 195, 298, 462
Sau3AI GATC 1 cut(s) 69
SchI GAGTC 1 cut(s) 35
SduI GDGCHC 1 cut(s) 441
SetI ASST 8 cut(s) 317, 325, 390, 395, 410, 525, 559, 567
SfaNI GCATC 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 101
Sse9I AATT 2 cut(s) 78, 533
StyI CCWWGG 1 cut(s) 204
TaiI ACGT 1 cut(s) 410
TaqI TCGA 3 cut(s) 72, 384, 489
TasI AATT 2 cut(s) 78, 533
TatI WGTACW 1 cut(s) 285
TauI GCSGC 5 cut(s) 151, 194, 197, 300, 464
TfiI GAWTC 3 cut(s) 74, 110, 185
TseI GCWGC 2 cut(s) 32, 151
TspDTI ATGAA 1 cut(s) 305
TspGWI ACGGA 1 cut(s) 79
XmnI GAANNNNTTC 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.