MD06G1002200.v1.1

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004333
Physical Location & Seq
Reverse (-)
235534 .. 238298
2765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1002200.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGGGAGTGTTTTGTCTTCCACTACAGAAAATCCTGACCCGCCCTCGTCCAACTCAACGATTCCCACTGATTCTCATAAGGCTGGCTCACCACCAAACTCATCAAATCCCCACCTGGCCGATTCAGATTCTAAAACCCCAGACAAGCAAAATCCAAAACATTCCTTAGATCCCCAATTGGGTAACAAAACCCTAGAAGAAGAGAAGCGTCAGGAAGATGAAGGCCAATCAGAGAATGTTATGGAGGAAGAAGAGGAAGAGGAGGAGGGAGAGTGTGGCTTTTGCTTGTTCATGAAAGGGGGAGGGTGCAAGGAGAGTTTCATAGCTTGGGAAGAGTGCGTTAGGGAGTCTGAGAAGACAAAGGAGGATGTTGTTGAAAAGTGCTTTGAGGTCACCGCAACATTGAAGAAGTGTATGCAGGCTCATTCAGATTACTATCTGCCCATTTTGAAGGCTGAGGAGGCAGCTGAGAAGGAGGTTGCCAAAGAATTGGAGAAGGAAAAGGATTCTGAGAGCTTGGAGAAAAATGCTACTTTGGAAAAAACGGATCAAAAGGGTGGAAGTTCCAGTGAAAAAGGAGACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

196

Amino Acids

21.52

Weight (kDa)

4.52

Isoelectric Point (pI)

67.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 90 - 163 6.9e-35 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 41, 396
AclWI GGATC 2 cut(s) 164, 555
AcoI YGGCCR 1 cut(s) 117
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 3 cut(s) 377, 406, 451
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 3 cut(s) 326, 467, 516
AluI AGCT 3 cut(s) 326, 467, 516
Alw26I GTCTC 1 cut(s) 573
AlwI GGATC 2 cut(s) 164, 555
AoxI GGCC 2 cut(s) 117, 223
ApeKI GCWGC 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 81, 385
BbsI GAAGAC 2 cut(s) 9, 362
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 1 cut(s) 476
BciT130I CCWGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 573
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 1 cut(s) 465
BlsI GCNGC 1 cut(s) 466
Bme1390I CCNGG 1 cut(s) 116
BmrFI CCNGG 1 cut(s) 116
BpiI GAAGAC 2 cut(s) 9, 362
Bpu10I CCTNAGC 1 cut(s) 456
BsaBI GATNNNNATC 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 567
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 1 cut(s) 116
BseGI GGATG 1 cut(s) 373
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMII CTCAG 4 cut(s) 342, 447, 459, 501
BseNI ACTGG 1 cut(s) 567
BseRI GAGGAG 3 cut(s) 275, 278, 473
BseXI GCAGC 1 cut(s) 476
BshFI GGCC 2 cut(s) 119, 225
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 573
BsnI GGCC 2 cut(s) 119, 225
Bsp143I GATC 2 cut(s) 169, 547
BspACI CCGC 2 cut(s) 41, 396
BspANI GGCC 2 cut(s) 119, 225
BspCNI CTCAG 4 cut(s) 343, 448, 460, 502
BspHI TCATGA 1 cut(s) 291
BspPI GGATC 2 cut(s) 164, 555
BsrI ACTGG 1 cut(s) 567
BssMI GATC 2 cut(s) 169, 547
Bst2UI CCWGG 1 cut(s) 116
Bst6I CTCTTC 4 cut(s) 195, 246, 252, 327
BstC8I GCNNGC 2 cut(s) 85, 420
BstDEI CTNAG 5 cut(s) 166, 351, 456, 468, 510
BstEII GGTNACC 1 cut(s) 391
BstF5I GGATG 1 cut(s) 373
BstKTI GATC 2 cut(s) 172, 550
BstMAI GTCTC 1 cut(s) 573
BstMBI GATC 2 cut(s) 169, 547
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstNI CCWGG 1 cut(s) 116
BstPI GGTNACC 1 cut(s) 391
BstSCI CCNGG 1 cut(s) 114
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 476
BstV2I GAAGAC 2 cut(s) 9, 362
BstX2I RGATCY 1 cut(s) 169
BstXI CCANNNNNNTGG 1 cut(s) 490
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 2 cut(s) 119, 225
BtsCI GGATG 1 cut(s) 373
BtsIMutI CAGTG 2 cut(s) 66, 574
Cac8I GCNNGC 2 cut(s) 85, 420
CciI TCATGA 1 cut(s) 291
CseI GACGC 1 cut(s) 197
CviAII CATG 1 cut(s) 292
DdeI CTNAG 5 cut(s) 166, 351, 456, 468, 510
DpnI GATC 2 cut(s) 171, 549
DpnII GATC 2 cut(s) 169, 547
EaeI YGGCCR 1 cut(s) 117
Eam1104I CTCTTC 4 cut(s) 195, 246, 252, 327
EarI CTCTTC 4 cut(s) 195, 246, 252, 327
Eco91I GGTNACC 1 cut(s) 391
EcoO65I GGTNACC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 114
FaeI CATG 1 cut(s) 295
FaiI YATR 5 cut(s) 78, 242, 293, 323, 416
FatI CATG 1 cut(s) 291
FauI CCCGC 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 465
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 1 cut(s) 465
FspBI CTAG 1 cut(s) 194
GluI GCNGC 1 cut(s) 465
HaeIII GGCC 2 cut(s) 119, 225
HgaI GACGC 1 cut(s) 197
Hin1II CATG 1 cut(s) 295
HinfI GANTC 6 cut(s) 61, 71, 122, 128, 347, 506
HphI GGTGA 2 cut(s) 81, 385
Hpy188I TCNGA 5 cut(s) 127, 232, 352, 430, 511
Hpy188III TCNNGA 3 cut(s) 35, 212, 292
HpyAV CCTTC 4 cut(s) 215, 445, 466, 490
HpyCH4V TGCA 2 cut(s) 309, 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 5 cut(s) 166, 351, 456, 468, 510
Hsp92II CATG 1 cut(s) 295
Kzo9I GATC 2 cut(s) 169, 547
LpnPI CCDG 8 cut(s) 48, 69, 101, 128, 153, 197, 404, 580
Lsp1109I GCAGC 1 cut(s) 476
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 2 cut(s) 182, 391
MalI GATC 2 cut(s) 171, 549
MboI GATC 2 cut(s) 169, 547
MfeI CAATTG 1 cut(s) 176
MflI RGATCY 1 cut(s) 169
MluCI AATT 3 cut(s) 176, 488, 583
MlyI GAGTC 1 cut(s) 356
MmeI TCCRAC 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 467
MspR9I CCNGG 1 cut(s) 116
MunI CAATTG 1 cut(s) 176
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 1 cut(s) 461
NdeII GATC 2 cut(s) 169, 547
NlaIII CATG 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 391
PagI TCATGA 1 cut(s) 291
PfeI GAWTC 5 cut(s) 61, 71, 122, 128, 506
PkrI GCNGC 1 cut(s) 466
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 114
PspEI GGTNACC 1 cut(s) 391
PspGI CCWGG 1 cut(s) 114
PsuI RGATCY 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 467
SatI GCNGC 1 cut(s) 465
Sau3AI GATC 2 cut(s) 169, 547
SchI GAGTC 1 cut(s) 356
ScrFI CCNGG 1 cut(s) 116
SetI ASST 6 cut(s) 117, 328, 393, 469, 480, 518
SfcI CTRYAG 1 cut(s) 24
Sse9I AATT 3 cut(s) 176, 488, 583
SsiI CCGC 2 cut(s) 41, 396
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 114
TasI AATT 3 cut(s) 176, 488, 583
TfiI GAWTC 5 cut(s) 61, 71, 122, 128, 506
TscAI CASTG 2 cut(s) 73, 574
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 4 cut(s) 234, 280, 308, 310
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 2 cut(s) 73, 574
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.