Rh7DG306600

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004333
Physical Location & Seq
Forward (+)
35779841 .. 35784866
5026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG306600.1

Sequence Viewer

Length: 408 bp
ATGGGAGGCGCTTCCATCACAACCGAACCTAACTCTTCCAACTCCACACTTCCGCTGCCTTCTCAGAAAAATGATGGCCAATCAGAGAATGGCAAAGAAGAAGATGCAGAGGAAGAGAAAGGTGAGTGTGGGTTTTGCTTATTCATGAAAGCAGGTCCGTGTGGAGAGAGATTCACAGCTTGGGAACAGTGCGTTAAAGAGTCTGAGAAGAACAAGGATTATGCTGCTGCAAAGTGTGTTGAGGTCTTCAAAACTTTGGATGAGTGTATGAAAGAGAATCAAGATTACTATGATTTTTTCTTGAGGGCCGAGAAAGCAGACTTGGAGAAGGAAGAGGATTTGATGAGAGAGAACTCAGAGCACAAAGCCAGTTCAATTGGGGATTCTACTGACAAGCAAGAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

135

Amino Acids

15.1

Weight (kDa)

4.45

Isoelectric Point (pI)

59.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 41 - 110 7.2e-25 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 143
AciI CCGC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 76
AgsI TTSAA 2 cut(s) 250, 375
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 1 cut(s) 179
AluI AGCT 1 cut(s) 179
Alw21I GWGCWC 1 cut(s) 363
AoxI GGCC 2 cut(s) 76, 306
ApeKI GCWGC 3 cut(s) 55, 224, 227
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 155, 306
AsuHPI GGTGA 1 cut(s) 134
AvaII GGWCC 1 cut(s) 155
BalI TGGCCA 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 3 cut(s) 42, 211, 214
BccI CCATC 2 cut(s) 23, 68
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 3 cut(s) 56, 225, 228
BlsI GCNGC 3 cut(s) 57, 226, 229
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 2 cut(s) 155, 306
BmsI GCATC 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 322
Bse1I ACTGG 1 cut(s) 369
BseGI GGATG 1 cut(s) 265
BseMII CTCAG 3 cut(s) 77, 195, 369
BseNI ACTGG 1 cut(s) 369
BseXI GCAGC 3 cut(s) 42, 211, 214
BshFI GGCC 2 cut(s) 78, 308
BsiHKAI GWGCWC 1 cut(s) 363
BsnI GGCC 2 cut(s) 78, 308
Bsp1286I GDGCHC 1 cut(s) 363
BspACI CCGC 1 cut(s) 53
BspANI GGCC 2 cut(s) 78, 308
BspCNI CTCAG 3 cut(s) 76, 196, 368
BspHI TCATGA 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 143
BsrI ACTGG 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 189
Bst6I CTCTTC 3 cut(s) 40, 108, 327
BstDEI CTNAG 3 cut(s) 63, 204, 355
BstF5I GGATG 1 cut(s) 265
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstV1I GCAGC 3 cut(s) 42, 211, 214
BstV2I GAAGAC 1 cut(s) 238
BsuRI GGCC 2 cut(s) 78, 308
BtsCI GGATG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 194
BveI ACCTGC 1 cut(s) 143
CciI TCATGA 1 cut(s) 144
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 155, 306
CviAII CATG 1 cut(s) 145
CviJI RGCY 4 cut(s) 78, 179, 308, 368
CviKI_1 RGCY 4 cut(s) 78, 179, 308, 368
DdeI CTNAG 3 cut(s) 63, 204, 355
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 3 cut(s) 40, 108, 327
EarI CTCTTC 3 cut(s) 40, 108, 327
Eco47I GGWCC 1 cut(s) 155
FaeI CATG 1 cut(s) 148
FaiI YATR 4 cut(s) 146, 222, 269, 291
FatI CATG 1 cut(s) 144
Fnu4HI GCNGC 3 cut(s) 56, 225, 228
FokI GGATG 1 cut(s) 272
Fsp4HI GCNGC 3 cut(s) 56, 225, 228
GlaI GCGC 1 cut(s) 10
GluI GCNGC 3 cut(s) 56, 225, 228
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 78, 308
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 148
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 4 cut(s) 171, 200, 277, 383
HphI GGTGA 1 cut(s) 134
Hpy188I TCNGA 4 cut(s) 66, 85, 205, 358
Hpy188III TCNNGA 3 cut(s) 145, 281, 301
HpyAV CCTTC 3 cut(s) 69, 322, 395
HpyCH4III ACNGT 1 cut(s) 189
HpyCH4V TGCA 2 cut(s) 107, 230
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 3 cut(s) 63, 204, 355
Hsp92II CATG 1 cut(s) 148
HspAI GCGC 1 cut(s) 9
LpnPI CCDG 2 cut(s) 138, 382
Lsp1109I GCAGC 3 cut(s) 42, 211, 214
LweI GCATC 1 cut(s) 94
MboII GAAGA 7 cut(s) 27, 110, 113, 125, 220, 238, 344
MfeI CAATTG 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 363
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 1 cut(s) 375
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 4 cut(s) 103, 235, 297, 328
Mox20I TGGCCA 1 cut(s) 78
MscI TGGCCA 1 cut(s) 78
MseI TTAA 1 cut(s) 195
Msp20I TGGCCA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 55
MunI CAATTG 1 cut(s) 375
MwoI GCNNNNNNNGC 1 cut(s) 314
NlaIII CATG 1 cut(s) 148
NmeAIII GCCGAG 1 cut(s) 334
PagI TCATGA 1 cut(s) 144
PfeI GAWTC 3 cut(s) 171, 277, 383
PkrI GCNGC 3 cut(s) 57, 226, 229
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 155, 306
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 3 cut(s) 56, 225, 228
Sau96I GGNCC 2 cut(s) 155, 306
SchI GAGTC 1 cut(s) 209
SduI GDGCHC 1 cut(s) 363
SetI ASST 6 cut(s) 31, 124, 157, 181, 246, 406
SfaNI GCATC 1 cut(s) 94
SinI GGWCC 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
Sse9I AATT 1 cut(s) 375
SsiI CCGC 1 cut(s) 53
TaaI ACNGT 1 cut(s) 189
TasI AATT 1 cut(s) 375
TfiI GAWTC 3 cut(s) 171, 277, 383
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 1 cut(s) 194
TseI GCWGC 3 cut(s) 55, 224, 227
TspDTI ATGAA 3 cut(s) 133, 161, 284
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 155
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.