pycom04g00420

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004333
Physical Location & Seq
Forward (+)
412381 .. 412914
534 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00420.1

Sequence Viewer

Length: 534 bp
ATGGCCGATTCAGCTTCTAAAACCCTAGAAGACCAACCTCCGAATCAGTCCCCAAACCCCCAAATGGATACCAAAACCGCAGAAGAAGTAGAAAAGTCCAAGCAAGAAGAATTAGAAAAGTCCAAGCAAGATGAAGTGAAATCGGAGAACGCATCAAAATCAGAAGGGGAAGCGGCGGCGGAGGACGAGGAAGAGGAGGAGGAGGCGGGAGAGTGTGGGTTCTGCTTGTTCATGAAAGGCGGCGGGTGCAAAGAGAGCTTCACGGCGTGGGAAGAATGCATCGAAGAGGCTGAGAAGAACAAGGAGGATATTGCGGAGAAATGCTTCGAGATCACTTCGGTTTTGAAGGAATGTATGATGGCACACTCCGATTACTACGAGCCCATACTCAAGGCCGAGAAGGCGGCCGAGGCAGAGGCTGTTAAGGAATTGGAGAAGGAAAAGGCTGCTGAGAGCTCCGAACAACGAAATGCCGCCGCCTCCTCGGAGAATTCCAAGCAGCATTCCGGTTCTAGCGGCAAGCAAGACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

178

Amino Acids

19.48

Weight (kDa)

4.41

Isoelectric Point (pI)

73.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 70 - 143 5.4e-35 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 3, 405
AcsI RAATTY 1 cut(s) 490
AdeI CACNNNGTG 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 1 cut(s) 346
AluBI AGCT 3 cut(s) 14, 258, 456
AluI AGCT 3 cut(s) 14, 258, 456
Alw21I GWGCWC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 419
AoxI GGCC 3 cut(s) 3, 393, 405
ApeKI GCWGC 2 cut(s) 446, 499
ApoI RAATTY 1 cut(s) 490
Asp700I GAANNNNTTC 1 cut(s) 323
BanII GRGCYC 2 cut(s) 384, 458
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 2 cut(s) 433, 511
BccI CCATC 1 cut(s) 352
BceAI ACGGC 1 cut(s) 279
BciVI GTATCC 1 cut(s) 61
BfaI CTAG 2 cut(s) 26, 513
BfuI GTATCC 1 cut(s) 61
BglI GCCNNNNNGGC 1 cut(s) 401
BisI GCNGC 9 cut(s) 174, 177, 241, 405, 447, 474, 477, 500, 517
BlsI GCNGC 9 cut(s) 175, 178, 242, 406, 448, 475, 478, 501, 518
BmsI GCATC 2 cut(s) 161, 288
BpiI GAAGAC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 374
BsaJI CCNNGG 2 cut(s) 408, 483
BsaWI WCCGGW 1 cut(s) 506
Bsc4I CCNNNNNNNGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 408, 483
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMII CTCAG 2 cut(s) 282, 441
BseRI GAGGAG 4 cut(s) 209, 212, 215, 472
BseX3I CGGCCG 1 cut(s) 405
BseXI GCAGC 2 cut(s) 433, 511
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 3 cut(s) 5, 395, 407
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 458
BsiSI CCGG 1 cut(s) 507
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 2 cut(s) 281, 502
BsnI GGCC 3 cut(s) 5, 395, 407
Bsp1286I GDGCHC 2 cut(s) 384, 458
Bsp143I GATC 1 cut(s) 330
BspANI GGCC 3 cut(s) 5, 395, 407
BspCNI CTCAG 2 cut(s) 283, 442
BspHI TCATGA 1 cut(s) 231
BssECI CCNNGG 2 cut(s) 408, 483
BssMI GATC 1 cut(s) 330
Bst6I CTCTTC 2 cut(s) 186, 279
BstC8I GCNNGC 1 cut(s) 521
BstDEI CTNAG 2 cut(s) 291, 450
BstKTI GATC 1 cut(s) 333
BstMBI GATC 1 cut(s) 330
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 5 cut(s) 11, 246, 255, 401, 410
BstV1I GCAGC 2 cut(s) 433, 511
BstV2I GAAGAC 1 cut(s) 36
BstZI CGGCCG 1 cut(s) 405
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 3 cut(s) 5, 395, 407
Cac8I GCNNGC 1 cut(s) 521
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 231
CviAII CATG 1 cut(s) 232
DdeI CTNAG 2 cut(s) 291, 450
DpnI GATC 1 cut(s) 332
DpnII GATC 1 cut(s) 330
DraIII CACNNNGTG 1 cut(s) 267
EaeI YGGCCR 2 cut(s) 3, 405
EagI CGGCCG 1 cut(s) 405
Eam1104I CTCTTC 2 cut(s) 186, 279
EarI CTCTTC 2 cut(s) 186, 279
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 456
EclXI CGGCCG 1 cut(s) 405
Eco24I GRGCYC 2 cut(s) 384, 458
Eco52I CGGCCG 1 cut(s) 405
Eco53kI GAGCTC 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 456
EcoRI GAATTC 1 cut(s) 490
EcoT22I ATGCAT 1 cut(s) 281
EcoT38I GRGCYC 2 cut(s) 384, 458
FaeI CATG 1 cut(s) 235
FaiI YATR 4 cut(s) 233, 356, 386, 532
FaqI GGGAC 1 cut(s) 34
FatI CATG 1 cut(s) 231
FauI CCCGC 2 cut(s) 199, 236
Fnu4HI GCNGC 9 cut(s) 174, 177, 241, 405, 447, 474, 477, 500, 517
FriOI GRGCYC 2 cut(s) 384, 458
Fsp4HI GCNGC 9 cut(s) 174, 177, 241, 405, 447, 474, 477, 500, 517
FspBI CTAG 2 cut(s) 26, 513
GluI GCNGC 9 cut(s) 174, 177, 241, 405, 447, 474, 477, 500, 517
HaeIII GGCC 3 cut(s) 5, 395, 407
HapII CCGG 1 cut(s) 507
Hin1II CATG 1 cut(s) 235
HinfI GANTC 2 cut(s) 8, 43
HpaII CCGG 1 cut(s) 507
Hpy188I TCNGA 6 cut(s) 42, 145, 163, 370, 460, 487
Hpy188III TCNNGA 2 cut(s) 232, 328
HpyAV CCTTC 4 cut(s) 158, 340, 394, 430
HpyCH4V TGCA 2 cut(s) 249, 279
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 246, 255, 401, 410
HpyF3I CTNAG 2 cut(s) 291, 450
Hsp92II CATG 1 cut(s) 235
Kzo9I GATC 1 cut(s) 330
LmnI GCTCC 1 cut(s) 461
LpnPI CCDG 1 cut(s) 520
Lsp1109I GCAGC 2 cut(s) 433, 511
LweI GCATC 2 cut(s) 161, 288
MaeI CTAG 2 cut(s) 26, 513
MalI GATC 1 cut(s) 332
MboI GATC 1 cut(s) 330
MboII GAAGA 7 cut(s) 41, 95, 119, 203, 284, 296, 307
MhlI GDGCHC 2 cut(s) 384, 458
MluCI AATT 3 cut(s) 110, 428, 490
Mph1103I ATGCAT 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 1 cut(s) 423
MspI CCGG 1 cut(s) 507
Mva1269I GAATGC 2 cut(s) 281, 502
MwoI GCNNNNNNNGC 5 cut(s) 11, 246, 255, 401, 410
NdeII GATC 1 cut(s) 330
NlaIII CATG 1 cut(s) 235
NmeAIII GCCGAG 2 cut(s) 421, 433
NsiI ATGCAT 1 cut(s) 281
PagI TCATGA 1 cut(s) 231
PctI GAATGC 2 cut(s) 281, 502
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 2 cut(s) 8, 43
PkrI GCNGC 9 cut(s) 175, 178, 242, 406, 448, 475, 478, 501, 518
Psp124BI GAGCTC 1 cut(s) 458
PstNI CAGNNNCTG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 458
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 9 cut(s) 174, 177, 241, 405, 447, 474, 477, 500, 517
Sau3AI GATC 1 cut(s) 330
SduI GDGCHC 2 cut(s) 384, 458
SetI ASST 4 cut(s) 16, 40, 260, 458
SfaNI GCATC 2 cut(s) 161, 288
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 3 cut(s) 110, 428, 490
SspMI CTAG 2 cut(s) 26, 513
SstI GAGCTC 1 cut(s) 458
TaqI TCGA 2 cut(s) 282, 327
TasI AATT 3 cut(s) 110, 428, 490
TauI GCSGC 7 cut(s) 176, 179, 243, 407, 476, 479, 519
TfiI GAWTC 2 cut(s) 8, 43
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseI GCWGC 2 cut(s) 446, 499
TspDTI ATGAA 3 cut(s) 147, 220, 248
XapI RAATTY 1 cut(s) 490
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 2 cut(s) 26, 513
Zsp2I ATGCAT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.