FvH4_5g26420

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004333
Physical Location & Seq
Forward (+)
17918737 .. 17923316
4580 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26420.t1

Sequence Viewer

Length: 435 bp
ATGGGAGGTGCTTCCATCACAGCTGGACCTAACTCTTCCAACTCCACACTTCAGCTCCCTTCTCAGCAAGATGACGACCAATCAGAGAATGTCAAAAAAGAAGAAGAAATAGAAGAATATGAAGAAGAAAATGAAGACGAAGAGAAAGGTGAGTGTGGATTTTGCTTATTCATGAAAGCAGGTCCGTGCGGAGAGAGATTCACAGCTTGGGAACAGTGCGTTAAAGATTCTGAGAAGAACAAGGATTATGCTGCTGTAAAGTGTGTCGAGGTTTTCAAAACTTTGAATGAGTGTATGAAGGAGAATCAAGAGTACTATGATTTTATTTTGAAGGCTGAGAAAGCAGAATTGGAGAAGAAAGAGGATTCTATCAGTGAGAGCTCGGAGCACAAAGCCAGTTCAATTGGGGATTCAACTGACAAGCAAGAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

145

Amino Acids

16.19

Weight (kDa)

4.33

Isoelectric Point (pI)

73.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 50 - 120 4.5e-24 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 170
AciI CCGC 1 cut(s) 189
AcuI CTGAAG 1 cut(s) 35
AfaI GTAC 1 cut(s) 314
AgsI TTSAA 5 cut(s) 277, 286, 331, 402, 414
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 4 cut(s) 23, 55, 206, 381
AluI AGCT 4 cut(s) 23, 55, 206, 381
Alw21I GWGCWC 2 cut(s) 383, 390
ApeKI GCWGC 1 cut(s) 251
AspS9I GGNCC 2 cut(s) 26, 182
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 2 cut(s) 26, 182
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 2 cut(s) 383, 390
BbvI GCAGC 1 cut(s) 238
BccI CCATC 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 170
BisI GCNGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 253
BmcAI AGTACT 1 cut(s) 314
Bme18I GGWCC 2 cut(s) 26, 182
BmgT120I GGNCC 2 cut(s) 26, 182
BpiI GAAGAC 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bse1I ACTGG 1 cut(s) 396
BseMII CTCAG 3 cut(s) 77, 222, 327
BseNI ACTGG 1 cut(s) 396
BseXI GCAGC 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 383, 390
Bsp1286I GDGCHC 2 cut(s) 383, 390
BspACI CCGC 1 cut(s) 189
BspCNI CTCAG 3 cut(s) 76, 223, 328
BspHI TCATGA 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 170
BsrI ACTGG 1 cut(s) 396
Bst4CI ACNGT 1 cut(s) 216
Bst6I CTCTTC 2 cut(s) 40, 135
BstDEI CTNAG 3 cut(s) 63, 231, 336
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstV1I GCAGC 1 cut(s) 238
BstV2I GAAGAC 1 cut(s) 141
BtsIMutI CAGTG 2 cut(s) 221, 379
BveI ACCTGC 1 cut(s) 170
CciI TCATGA 1 cut(s) 171
Cfr13I GGNCC 2 cut(s) 26, 182
Csp6I GTAC 1 cut(s) 313
CviAII CATG 1 cut(s) 172
CviJI RGCY 6 cut(s) 23, 55, 206, 335, 381, 395
CviKI_1 RGCY 6 cut(s) 23, 55, 206, 335, 381, 395
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 3 cut(s) 63, 231, 336
Eam1104I CTCTTC 2 cut(s) 40, 135
EarI CTCTTC 2 cut(s) 40, 135
Ecl136II GAGCTC 1 cut(s) 381
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 2 cut(s) 26, 182
Eco53kI GAGCTC 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 383
FaeI CATG 1 cut(s) 175
FaiI YATR 5 cut(s) 120, 173, 249, 296, 318
FatI CATG 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 252
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 1 cut(s) 252
GluI GCNGC 1 cut(s) 252
Hin1II CATG 1 cut(s) 175
HinfI GANTC 5 cut(s) 198, 227, 304, 365, 410
HphI GGTGA 1 cut(s) 161
Hpy188I TCNGA 3 cut(s) 85, 232, 385
Hpy188III TCNNGA 2 cut(s) 172, 308
HpyAV CCTTC 4 cut(s) 69, 292, 325, 422
HpyCH4III ACNGT 1 cut(s) 216
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 3 cut(s) 63, 231, 336
Hsp92II CATG 1 cut(s) 175
LmnI GCTCC 2 cut(s) 60, 385
LpnPI CCDG 3 cut(s) 9, 165, 409
Lsp1109I GCAGC 1 cut(s) 238
MfeI CAATTG 1 cut(s) 402
MhlI GDGCHC 2 cut(s) 383, 390
MluCI AATT 2 cut(s) 347, 402
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 2 cut(s) 262, 355
MseI TTAA 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 23
MunI CAATTG 1 cut(s) 402
MwoI GCNNNNNNNGC 1 cut(s) 341
NlaIII CATG 1 cut(s) 175
PagI TCATGA 1 cut(s) 171
PfeI GAWTC 5 cut(s) 198, 227, 304, 365, 410
PkrI GCNGC 1 cut(s) 253
Psp124BI GAGCTC 1 cut(s) 383
PspPI GGNCC 2 cut(s) 26, 182
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SacI GAGCTC 1 cut(s) 383
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 1 cut(s) 252
Sau96I GGNCC 2 cut(s) 26, 182
ScaI AGTACT 1 cut(s) 314
SduI GDGCHC 2 cut(s) 383, 390
SinI GGWCC 2 cut(s) 26, 182
Sse9I AATT 2 cut(s) 347, 402
SsiI CCGC 1 cut(s) 189
SstI GAGCTC 1 cut(s) 383
TaaI ACNGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 267
TasI AATT 2 cut(s) 347, 402
TatI WGTACW 1 cut(s) 312
TfiI GAWTC 5 cut(s) 198, 227, 304, 365, 410
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 2 cut(s) 221, 379
TseI GCWGC 1 cut(s) 251
TspDTI ATGAA 5 cut(s) 135, 147, 160, 188, 311
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 2 cut(s) 221, 379
VpaK11BI GGWCC 2 cut(s) 26, 182
ZrmI AGTACT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.