MD04G1004200.v1.1

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004333
Physical Location & Seq
Forward (+)
556473 .. 556994
522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1004200.v1.1.491

Sequence Viewer

Length: 522 bp
ATGGCCGATTCAGCTTCTAAAACCCTAGAAGACCAACCTCCAAATCAGTCCTCAAACCCCCAAATGGATACGAAAACCGTAGAAGAAGAAGAGGAGAAGTCCCAGCAAGAAGAAGTGAAATCGGAAAACGCGTCAAAAGCAGAAGGGGAAGCGGCGGCGGAGGATGAGGAAGAGGAGGAGGAGGCGGGAGAGTGTGGGTTCTGCTTGTTCATGAAAGGCGGCGGGTGCAAAGAGAGCTTCACGGCGTGGGAAGAATGCATCGAAGAGGCTGAGAAGAACAAGGAGGATATTGTGGAGAAATGCTTCGAGATCACTTCGGTTTTGAAGGAATGTATGATGGCTCACTCCGATTACTACGAGCCCATACTCAAGGCCGAGAAGGCGGCCGAGGCAGAGGCTGTTAAGGAATTGGAGAAGGAAAAGGCTGCTGAGAGCTCCGAACAACGAAATGCCGCCGTCGCCTCGGAGAATTCCCAGCAGCATTCCGGTTCTAGCGGCAAGCAAGACGCATTGCCCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

174

Amino Acids

18.96

Weight (kDa)

4.28

Isoelectric Point (pI)

77.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 63 - 136 1.3e-35 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 131
AciI CCGC 9 cut(s) 152, 155, 158, 185, 219, 222, 383, 453, 495
AcoI YGGCCR 2 cut(s) 3, 384
AcsI RAATTY 1 cut(s) 469
AdeI CACNNNGTG 1 cut(s) 246
AfiI CCNNNNNNNGG 1 cut(s) 64
AflIII ACRYGT 1 cut(s) 129
AgsI TTSAA 1 cut(s) 325
AluBI AGCT 3 cut(s) 14, 237, 435
AluI AGCT 3 cut(s) 14, 237, 435
Alw21I GWGCWC 1 cut(s) 437
AlwNI CAGNNNCTG 1 cut(s) 398
AoxI GGCC 3 cut(s) 3, 372, 384
ApeKI GCWGC 2 cut(s) 425, 478
ApoI RAATTY 1 cut(s) 469
Asp700I GAANNNNTTC 1 cut(s) 302
AsuC2I CCSGG 1 cut(s) 516
BanII GRGCYC 2 cut(s) 363, 437
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 2 cut(s) 412, 490
BccI CCATC 1 cut(s) 331
BceAI ACGGC 2 cut(s) 258, 440
BciVI GTATCC 1 cut(s) 61
BcnI CCSGG 1 cut(s) 516
BfaI CTAG 2 cut(s) 26, 492
BfuI GTATCC 1 cut(s) 61
BglI GCCNNNNNGGC 1 cut(s) 380
BisI GCNGC 8 cut(s) 153, 156, 220, 384, 426, 453, 479, 496
BlsI GCNGC 8 cut(s) 154, 157, 221, 385, 427, 454, 480, 497
Bme1390I CCNGG 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 516
BmsI GCATC 1 cut(s) 267
BpiI GAAGAC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 516
BsaJI CCNNGG 2 cut(s) 387, 462
BsaWI WCCGGW 1 cut(s) 485
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 509
BseDI CCNNGG 2 cut(s) 387, 462
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 509
BseMII CTCAG 2 cut(s) 261, 420
BseRI GAGGAG 4 cut(s) 107, 188, 191, 194
BseX3I CGGCCG 1 cut(s) 384
BseXI GCAGC 2 cut(s) 412, 490
BseYI CCCAGC 2 cut(s) 102, 474
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 3 cut(s) 5, 374, 386
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 2 cut(s) 486, 516
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 85
BsmI GAATGC 2 cut(s) 260, 481
BsnI GGCC 3 cut(s) 5, 374, 386
Bsp1286I GDGCHC 2 cut(s) 363, 437
Bsp143I GATC 1 cut(s) 309
BspACI CCGC 9 cut(s) 152, 155, 158, 185, 219, 222, 383, 453, 495
BspANI GGCC 3 cut(s) 5, 374, 386
BspCNI CTCAG 2 cut(s) 262, 421
BspFNI CGCG 1 cut(s) 131
BspHI TCATGA 1 cut(s) 210
BsrDI GCAATG 1 cut(s) 509
BssECI CCNNGG 2 cut(s) 387, 462
BssMI GATC 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 3 cut(s) 84, 165, 258
BstC8I GCNNGC 1 cut(s) 500
BstDEI CTNAG 2 cut(s) 270, 429
BstF5I GGATG 1 cut(s) 169
BstFNI CGCG 1 cut(s) 131
BstKTI GATC 1 cut(s) 312
BstMBI GATC 1 cut(s) 309
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 7 cut(s) 11, 137, 225, 234, 380, 389, 458
BstSCI CCNGG 1 cut(s) 514
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 412, 490
BstV2I GAAGAC 1 cut(s) 36
BstZI CGGCCG 1 cut(s) 384
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 3 cut(s) 5, 374, 386
BtsCI GGATG 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 500
CaiI CAGNNNCTG 1 cut(s) 398
CciI TCATGA 1 cut(s) 210
CseI GACGC 2 cut(s) 120, 515
CviAII CATG 1 cut(s) 211
DdeI CTNAG 2 cut(s) 270, 429
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
DraIII CACNNNGTG 1 cut(s) 246
EaeI YGGCCR 2 cut(s) 3, 384
EagI CGGCCG 1 cut(s) 384
Eam1104I CTCTTC 3 cut(s) 84, 165, 258
EarI CTCTTC 3 cut(s) 84, 165, 258
EciI GGCGGA 1 cut(s) 173
Ecl136II GAGCTC 1 cut(s) 435
EclXI CGGCCG 1 cut(s) 384
Eco24I GRGCYC 2 cut(s) 363, 437
Eco52I CGGCCG 1 cut(s) 384
Eco53kI GAGCTC 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 435
EcoRI GAATTC 1 cut(s) 469
EcoT22I ATGCAT 1 cut(s) 260
EcoT38I GRGCYC 2 cut(s) 363, 437
FaeI CATG 1 cut(s) 214
FaiI YATR 3 cut(s) 212, 335, 365
FaqI GGGAC 1 cut(s) 85
FatI CATG 1 cut(s) 210
FauI CCCGC 2 cut(s) 178, 215
Fnu4HI GCNGC 8 cut(s) 153, 156, 220, 384, 426, 453, 479, 496
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 2 cut(s) 363, 437
Fsp4HI GCNGC 8 cut(s) 153, 156, 220, 384, 426, 453, 479, 496
FspBI CTAG 2 cut(s) 26, 492
GluI GCNGC 8 cut(s) 153, 156, 220, 384, 426, 453, 479, 496
GsaI CCCAGC 2 cut(s) 106, 478
HaeIII GGCC 3 cut(s) 5, 374, 386
HapII CCGG 2 cut(s) 486, 516
HgaI GACGC 2 cut(s) 120, 515
Hin1II CATG 1 cut(s) 214
HinfI GANTC 1 cut(s) 8
HpaII CCGG 2 cut(s) 486, 516
Hpy188I TCNGA 4 cut(s) 124, 349, 439, 466
Hpy188III TCNNGA 2 cut(s) 211, 307
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 4 cut(s) 137, 319, 373, 409
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4V TGCA 2 cut(s) 228, 258
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 137, 225, 234, 380, 389, 458
HpyF3I CTNAG 2 cut(s) 270, 429
Hsp92II CATG 1 cut(s) 214
Kzo9I GATC 1 cut(s) 309
LmnI GCTCC 1 cut(s) 440
LpnPI CCDG 3 cut(s) 116, 488, 499
Lsp1109I GCAGC 2 cut(s) 412, 490
LweI GCATC 1 cut(s) 267
MaeI CTAG 2 cut(s) 26, 492
MalI GATC 1 cut(s) 311
MboI GATC 1 cut(s) 309
MboII GAAGA 9 cut(s) 41, 95, 98, 101, 122, 182, 263, 275, 286
MhlI GDGCHC 2 cut(s) 363, 437
MluCI AATT 2 cut(s) 407, 469
MluI ACGCGT 1 cut(s) 129
Mph1103I ATGCAT 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 1 cut(s) 402
MspI CCGG 2 cut(s) 486, 516
MspR9I CCNGG 1 cut(s) 516
Mva1269I GAATGC 2 cut(s) 260, 481
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 7 cut(s) 11, 137, 225, 234, 380, 389, 458
NciI CCSGG 1 cut(s) 516
NdeII GATC 1 cut(s) 309
NlaIII CATG 1 cut(s) 214
NmeAIII GCCGAG 2 cut(s) 400, 412
NsiI ATGCAT 1 cut(s) 260
PagI TCATGA 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PctI GAATGC 2 cut(s) 260, 481
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 8 cut(s) 154, 157, 221, 385, 427, 454, 480, 497
Psp124BI GAGCTC 1 cut(s) 437
PspFI CCCAGC 2 cut(s) 102, 474
PstNI CAGNNNCTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 437
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 8 cut(s) 153, 156, 220, 384, 426, 453, 479, 496
Sau3AI GATC 1 cut(s) 309
ScrFI CCNGG 1 cut(s) 516
SduI GDGCHC 2 cut(s) 363, 437
SetI ASST 4 cut(s) 16, 40, 239, 437
SfaNI GCATC 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 407, 469
SsiI CCGC 9 cut(s) 152, 155, 158, 185, 219, 222, 383, 453, 495
SspMI CTAG 2 cut(s) 26, 492
SstI GAGCTC 1 cut(s) 437
StyD4I CCNGG 1 cut(s) 514
TaaI ACNGT 1 cut(s) 79
TaqI TCGA 2 cut(s) 261, 306
TasI AATT 2 cut(s) 407, 469
TauI GCSGC 6 cut(s) 155, 158, 222, 386, 455, 498
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TseI GCWGC 2 cut(s) 425, 478
TspDTI ATGAA 2 cut(s) 199, 227
XapI RAATTY 1 cut(s) 469
XmnI GAANNNNTTC 1 cut(s) 302
XspI CTAG 2 cut(s) 26, 492
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.