RLG00000002498

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004333
Physical Location & Seq
Reverse (-)
35295529 .. 35297971
2443 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002498

Sequence Viewer

Length: 594 bp
ATGAAAACAGCCAGTATTGCTTTTTATTCTCCCCAAAAACATAACCGCAACGACGTCGTTTTGCGACTTCGATTAGCCCTCGCTTCGCTTCACCGGCTTGGCCTCCGCCGCCGTTCAAGGAACTTCCGCTGCCGGAAAGCTCAGCGCGTCACACTTTCTTTATGTTTAAGAGGGATGGGAGGCGCTGCTATCACAACCGAACCTAACTCTTCCAACTCCACGCTTCCGCTGCCTTCTCAGAAAAATGACGGCCAATCAGAGAATGTCAAAGAAGAAGAAGAAGAAGAAAATGCAGAGGAAGAGAAAGGTGAGTGTGGGTTTTGCTTATTCATGAAAGCAGGTCCGTGTGGAGAGAGATTCACAGCTTGGGAACGGTGCGTTAAAGAGTCTGAGAAGAACAAGGATTATGCTGCTGCAAAGTGTGTTGAGGTCTTCAAAACTTTGGATGAGTGTATGAAAGAGAATCAAGATTACTATGATTTTTTCTTGAAGGCTGAGAGAGCAGAATTGGAGAAGGAAGAGGATTCGATGAGTGAGAGCTCAGAGCACAAAGCCAGTTCAATTGGGGATTCTACTGACAAGCAAGAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

198

Amino Acids

22.35

Weight (kDa)

5.62

Isoelectric Point (pI)

75.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 103 - 172 7.3e-24 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 57
Acc36I ACCTGC 1 cut(s) 329
AccII CGCG 1 cut(s) 147
AciI CCGC 5 cut(s) 46, 106, 109, 127, 227
AcoI YGGCCR 1 cut(s) 250
AcyI GRCGYC 1 cut(s) 54
AgsI TTSAA 4 cut(s) 117, 436, 490, 561
AluBI AGCT 3 cut(s) 140, 365, 540
AluI AGCT 3 cut(s) 140, 365, 540
Alw21I GWGCWC 2 cut(s) 542, 549
AoxI GGCC 2 cut(s) 100, 250
ApeKI GCWGC 5 cut(s) 129, 185, 229, 410, 413
AspLEI GCGC 2 cut(s) 147, 185
AspS9I GGNCC 1 cut(s) 341
AsuHPI GGTGA 2 cut(s) 83, 320
AvaII GGWCC 1 cut(s) 341
BanII GRGCYC 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 424
Bbv12I GWGCWC 2 cut(s) 542, 549
BbvI GCAGC 5 cut(s) 116, 172, 216, 397, 400
BccI CCATC 1 cut(s) 169
BceAI ACGGC 2 cut(s) 96, 265
BcgI CGANNNNNNTGC 2 cut(s) 37, 71
BfoI RGCGCY 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 329
BisI GCNGC 6 cut(s) 109, 130, 186, 230, 411, 414
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 6 cut(s) 110, 131, 187, 231, 412, 415
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 341
BpiI GAAGAC 1 cut(s) 424
Bpu1102I GCTNAGC 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 54
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 2 cut(s) 12, 555
BseGI GGATG 2 cut(s) 180, 451
BseMII CTCAG 5 cut(s) 155, 251, 381, 486, 555
BseNI ACTGG 2 cut(s) 12, 555
BseXI GCAGC 5 cut(s) 116, 172, 216, 397, 400
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 2 cut(s) 102, 252
BsiHKAI GWGCWC 2 cut(s) 542, 549
BsiSI CCGG 2 cut(s) 94, 133
BsnI GGCC 2 cut(s) 102, 252
Bsp1286I GDGCHC 2 cut(s) 542, 549
Bsp1720I GCTNAGC 1 cut(s) 141
BspACI CCGC 5 cut(s) 46, 106, 109, 127, 227
BspANI GGCC 2 cut(s) 102, 252
BspCNI CTCAG 5 cut(s) 154, 250, 382, 487, 554
BspFNI CGCG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 2 cut(s) 12, 555
BssAI RCCGGY 1 cut(s) 93
BssNI GRCGYC 1 cut(s) 54
Bst4CI ACNGT 1 cut(s) 375
Bst6I CTCTTC 3 cut(s) 214, 294, 513
BstACI GRCGYC 1 cut(s) 54
BstDEI CTNAG 5 cut(s) 141, 237, 390, 495, 541
BstF5I GGATG 2 cut(s) 180, 451
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 2 cut(s) 147, 185
BstMWI GCNNNNNNNGC 5 cut(s) 17, 94, 108, 229, 500
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 5 cut(s) 116, 172, 216, 397, 400
BstV2I GAAGAC 1 cut(s) 424
BsuRI GGCC 2 cut(s) 102, 252
BtsCI GGATG 2 cut(s) 180, 451
BveI ACCTGC 1 cut(s) 329
CciI TCATGA 1 cut(s) 330
CfoI GCGC 2 cut(s) 147, 185
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 341
CseI GACGC 1 cut(s) 136
CviAII CATG 1 cut(s) 331
DdeI CTNAG 5 cut(s) 141, 237, 390, 495, 541
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 3 cut(s) 214, 294, 513
EarI CTCTTC 3 cut(s) 214, 294, 513
EciI GGCGGA 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 540
Eco24I GRGCYC 1 cut(s) 542
Eco47I GGWCC 1 cut(s) 341
Eco53kI GAGCTC 1 cut(s) 540
EcoICRI GAGCTC 1 cut(s) 540
EcoT38I GRGCYC 1 cut(s) 542
FaeI CATG 1 cut(s) 334
FaiI YATR 6 cut(s) 42, 163, 332, 408, 455, 477
FatI CATG 1 cut(s) 330
Fnu4HI GCNGC 6 cut(s) 109, 130, 186, 230, 411, 414
FokI GGATG 2 cut(s) 187, 458
FriOI GRGCYC 1 cut(s) 542
Fsp4HI GCNGC 6 cut(s) 109, 130, 186, 230, 411, 414
GlaI GCGC 2 cut(s) 146, 184
GluI GCNGC 6 cut(s) 109, 130, 186, 230, 411, 414
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 2 cut(s) 102, 252
HapII CCGG 2 cut(s) 94, 133
HgaI GACGC 1 cut(s) 136
HhaI GCGC 2 cut(s) 147, 185
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 1 cut(s) 334
Hin6I GCGC 2 cut(s) 145, 183
HinP1I GCGC 2 cut(s) 145, 183
HinfI GANTC 5 cut(s) 357, 386, 463, 524, 569
HpaII CCGG 2 cut(s) 94, 133
HphI GGTGA 2 cut(s) 83, 320
Hpy188I TCNGA 4 cut(s) 240, 259, 391, 544
Hpy188III TCNNGA 3 cut(s) 331, 467, 487
Hpy99I CGWCG 2 cut(s) 56, 59
HpyAV CCTTC 4 cut(s) 243, 484, 508, 581
HpyCH4III ACNGT 1 cut(s) 375
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 2 cut(s) 293, 416
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 94, 108, 229, 500
HpyF3I CTNAG 5 cut(s) 141, 237, 390, 495, 541
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 1 cut(s) 334
HspAI GCGC 2 cut(s) 145, 183
LpnPI CCDG 5 cut(s) 25, 107, 146, 324, 568
Lsp1109I GCAGC 5 cut(s) 116, 172, 216, 397, 400
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 148
MfeI CAATTG 1 cut(s) 561
MhlI GDGCHC 2 cut(s) 542, 549
MluCI AATT 2 cut(s) 506, 561
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 7 cut(s) 89, 113, 164, 173, 289, 421, 514
MseI TTAA 2 cut(s) 167, 381
MspA1I CMGCKG 2 cut(s) 129, 229
MspI CCGG 2 cut(s) 94, 133
MunI CAATTG 1 cut(s) 561
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 5 cut(s) 17, 94, 108, 229, 500
NlaIII CATG 1 cut(s) 334
NmuCI GTSAC 1 cut(s) 148
PagI TCATGA 1 cut(s) 330
PfeI GAWTC 4 cut(s) 357, 463, 524, 569
PkrI GCNGC 6 cut(s) 110, 131, 187, 231, 412, 415
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 542
PspPI GGNCC 1 cut(s) 341
SacI GAGCTC 1 cut(s) 542
SaqAI TTAA 2 cut(s) 167, 381
SatI GCNGC 6 cut(s) 109, 130, 186, 230, 411, 414
Sau96I GGNCC 1 cut(s) 341
SchI GAGTC 1 cut(s) 395
SduI GDGCHC 2 cut(s) 542, 549
SetI ASST 9 cut(s) 57, 142, 205, 310, 343, 367, 432, 542, 592
SinI GGWCC 1 cut(s) 341
Sse9I AATT 2 cut(s) 506, 561
SsiI CCGC 5 cut(s) 46, 106, 109, 127, 227
SstI GAGCTC 1 cut(s) 542
TaaI ACNGT 1 cut(s) 375
TaiI ACGT 1 cut(s) 57
TaqI TCGA 2 cut(s) 70, 527
TasI AATT 2 cut(s) 506, 561
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 4 cut(s) 357, 463, 524, 569
Tru1I TTAA 2 cut(s) 167, 381
Tru9I TTAA 2 cut(s) 167, 381
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 5 cut(s) 129, 185, 229, 410, 413
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 4 cut(s) 17, 319, 347, 470
TspGWI ACGGA 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 341
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.