Rh7CG326000

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0004333
Physical Location & Seq
Forward (+)
35684081 .. 35684509
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG326000.1

Sequence Viewer

Length: 429 bp
ATGGGAGGCGCTTCCATCACAACCGAACCTAACTCTTCCAACTCCACACTTCCGCTGCCTTCTCAGAAAAATGATGGCCAATCAGAGAATGGCAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGCACAGGAAGAGAAAGGTGAGTGTGGGTTTTGCTTATTCATGAAAGCAGGTCCGTGTGGAGAGAGATTCACAGCTTGGGAACAGTGCGTTAAAGAGTCTGAGAAGAACAAGGATTATGCTGCTGCAAAGTGTGTTGAGGTCTTCAAAACTTTGGATGAGTGTATGAAAGAGAATCAAGATTACTATGATTTTTTCTTGAGGGCCGAGAAAGCAGAATTGGAGAAGGAAGAGGATTTGATGAGAGAGAGCTCAGAGCACAAAGCCAGTTCAATTGGGGATTCTACTGACAAGCAAGAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

142

Amino Acids

15.99

Weight (kDa)

4.32

Isoelectric Point (pI)

75.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 48 - 117 7e-25 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 164
AciI CCGC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 76
AgsI TTSAA 2 cut(s) 271, 396
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 2 cut(s) 200, 375
AluI AGCT 2 cut(s) 200, 375
Alw21I GWGCWC 2 cut(s) 377, 384
AoxI GGCC 2 cut(s) 76, 327
ApeKI GCWGC 3 cut(s) 55, 245, 248
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 176, 327
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 176
BalI TGGCCA 1 cut(s) 78
BanII GRGCYC 1 cut(s) 377
BbsI GAAGAC 1 cut(s) 259
Bbv12I GWGCWC 2 cut(s) 377, 384
BbvI GCAGC 3 cut(s) 42, 232, 235
BccI CCATC 2 cut(s) 23, 68
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 164
BisI GCNGC 3 cut(s) 56, 246, 249
BlsI GCNGC 3 cut(s) 57, 247, 250
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 2 cut(s) 176, 327
BmsI GCATC 1 cut(s) 115
BpiI GAAGAC 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 390
BseGI GGATG 1 cut(s) 286
BseMII CTCAG 3 cut(s) 77, 216, 390
BseNI ACTGG 1 cut(s) 390
BseXI GCAGC 3 cut(s) 42, 232, 235
BshFI GGCC 2 cut(s) 78, 329
BsiHKAI GWGCWC 2 cut(s) 377, 384
BsnI GGCC 2 cut(s) 78, 329
Bsp1286I GDGCHC 2 cut(s) 377, 384
BspACI CCGC 1 cut(s) 53
BspANI GGCC 2 cut(s) 78, 329
BspCNI CTCAG 3 cut(s) 76, 217, 389
BspHI TCATGA 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 164
BsrI ACTGG 1 cut(s) 390
Bst4CI ACNGT 1 cut(s) 210
Bst6I CTCTTC 3 cut(s) 40, 129, 348
BstDEI CTNAG 3 cut(s) 63, 225, 376
BstF5I GGATG 1 cut(s) 286
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstV1I GCAGC 3 cut(s) 42, 232, 235
BstV2I GAAGAC 1 cut(s) 259
BsuRI GGCC 2 cut(s) 78, 329
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 215
BveI ACCTGC 1 cut(s) 164
CciI TCATGA 1 cut(s) 165
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 176, 327
CviAII CATG 1 cut(s) 166
CviJI RGCY 5 cut(s) 78, 200, 329, 375, 389
CviKI_1 RGCY 5 cut(s) 78, 200, 329, 375, 389
DdeI CTNAG 3 cut(s) 63, 225, 376
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 3 cut(s) 40, 129, 348
EarI CTCTTC 3 cut(s) 40, 129, 348
Ecl136II GAGCTC 1 cut(s) 375
Eco24I GRGCYC 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 375
EcoICRI GAGCTC 1 cut(s) 375
EcoT38I GRGCYC 1 cut(s) 377
FaeI CATG 1 cut(s) 169
FaiI YATR 4 cut(s) 167, 243, 290, 312
FatI CATG 1 cut(s) 165
Fnu4HI GCNGC 3 cut(s) 56, 246, 249
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 3 cut(s) 56, 246, 249
GlaI GCGC 1 cut(s) 10
GluI GCNGC 3 cut(s) 56, 246, 249
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 78, 329
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 169
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 4 cut(s) 192, 221, 298, 404
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 4 cut(s) 66, 85, 226, 379
Hpy188III TCNNGA 3 cut(s) 166, 302, 322
HpyAV CCTTC 3 cut(s) 69, 343, 416
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4V TGCA 2 cut(s) 128, 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 3 cut(s) 63, 225, 376
Hsp92II CATG 1 cut(s) 169
HspAI GCGC 1 cut(s) 9
LpnPI CCDG 3 cut(s) 116, 159, 403
Lsp1109I GCAGC 3 cut(s) 42, 232, 235
LweI GCATC 1 cut(s) 115
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 2 cut(s) 377, 384
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 2 cut(s) 341, 396
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 3 cut(s) 256, 318, 349
Mox20I TGGCCA 1 cut(s) 78
MscI TGGCCA 1 cut(s) 78
MseI TTAA 1 cut(s) 216
Msp20I TGGCCA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 55
MunI CAATTG 1 cut(s) 396
MwoI GCNNNNNNNGC 1 cut(s) 335
NlaIII CATG 1 cut(s) 169
NmeAIII GCCGAG 1 cut(s) 355
PagI TCATGA 1 cut(s) 165
PfeI GAWTC 3 cut(s) 192, 298, 404
PkrI GCNGC 3 cut(s) 57, 247, 250
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
Psp124BI GAGCTC 1 cut(s) 377
PspPI GGNCC 2 cut(s) 176, 327
SacI GAGCTC 1 cut(s) 377
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 3 cut(s) 56, 246, 249
Sau96I GGNCC 2 cut(s) 176, 327
SchI GAGTC 1 cut(s) 230
SduI GDGCHC 2 cut(s) 377, 384
SetI ASST 7 cut(s) 31, 145, 178, 202, 267, 377, 427
SfaNI GCATC 1 cut(s) 115
SinI GGWCC 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
Sse9I AATT 2 cut(s) 341, 396
SsiI CCGC 1 cut(s) 53
SstI GAGCTC 1 cut(s) 377
TaaI ACNGT 1 cut(s) 210
TasI AATT 2 cut(s) 341, 396
TfiI GAWTC 3 cut(s) 192, 298, 404
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 1 cut(s) 215
TseI GCWGC 3 cut(s) 55, 245, 248
TspDTI ATGAA 3 cut(s) 154, 182, 305
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.