Rh1BG134700

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004333
Physical Location & Seq
Forward (+)
22580977 .. 22582290
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG134700.1

Sequence Viewer

Length: 597 bp
ATGGGTGCTTCCAACTCCATTGACAGACCAGCACCAGACTCATCAGAGGCGTCTCCCCCACCAAAGACCGATTCAATTCCCTCCTCCGTCTCCATCTCCATGTCCGATTCGGCTTCCAAACCCCAATCGGAAGACCAACCCCCGCCGCAGCCCCAATCGGACACAAAACCCTCCGAAGAAGAATCGGCGGCGGCGAAGCCCAAGGAAGACGATGCAGTCCAATCGGAGGAGGGGGAGGAGGAAGATGAGGAGAAGGGGGAGTGCGGGTTCTGCTTGTACATGAAAGGCGGCGGGTGCAAAGAGAGCTTTGTAGCTTGGGAAAACTGCATTGAAGAGGCGGAGAAGACGAACGAGGACATTGCTCAGAAGTGCTTCGACCTCACCTCCGCTCTCAGGACGTGTATGCTGGAACATTCCGATTACTACGAGCCCATTCTCAGGGCGGAGAAGGCCGCCGAGGAAGAAGCCATCAAGGAACTCGACAAGGAAAAGAAGGAAGAGAAAGAGAAGGAGAAGGAGAAGGAGGTGGTTATAGAATTGGAGAACGAAAAGGCTCCAGCTTCTGAGGTGGAGGAGAGTTCCGGTGGGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

198

Amino Acids

21.73

Weight (kDa)

4.31

Isoelectric Point (pI)

84.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 86 - 159 1.6e-34 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccBSI CCGCTC 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 3 cut(s) 226, 393, 438
AflIII ACRYGT 1 cut(s) 398
AgsI TTSAA 2 cut(s) 75, 332
AjiI CACGTC 1 cut(s) 399
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 3 cut(s) 306, 314, 560
AluI AGCT 3 cut(s) 306, 314, 560
Alw26I GTCTC 2 cut(s) 57, 94
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 1 cut(s) 450
ApeKI GCWGC 1 cut(s) 148
Asp700I GAANNNNTTC 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 373
BanII GRGCYC 1 cut(s) 432
BbsI GAAGAC 3 cut(s) 138, 213, 350
BbvI GCAGC 1 cut(s) 160
BccI CCATC 2 cut(s) 101, 476
BcgI CGANNNNNNTGC 4 cut(s) 204, 238, 341, 375
BcoDI GTCTC 2 cut(s) 57, 94
BisI GCNGC 6 cut(s) 146, 149, 189, 192, 289, 453
BlsI GCNGC 6 cut(s) 147, 150, 190, 193, 290, 454
BmgBI CACGTC 1 cut(s) 399
BmiI GGNNCC 1 cut(s) 555
BmsI GCATC 1 cut(s) 202
BpiI GAAGAC 3 cut(s) 138, 213, 350
BpmI CTGGAG 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 201, 456
BsaWI WCCGGW 1 cut(s) 581
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 368, 398
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 393, 438
Bse3DI GCAATG 1 cut(s) 357
BseDI CCNNGG 2 cut(s) 201, 456
BseLI CCNNNNNNNGG 3 cut(s) 226, 393, 438
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 4 cut(s) 377, 406, 451, 555
BseRI GAGGAG 5 cut(s) 73, 242, 251, 263, 587
BseXI GCAGC 1 cut(s) 160
BshFI GGCC 1 cut(s) 452
BsiSI CCGG 1 cut(s) 582
BslI CCNNNNNNNGG 3 cut(s) 226, 393, 438
BsmAI GTCTC 2 cut(s) 57, 94
BsmBI CGTCTC 2 cut(s) 57, 94
BsnI GGCC 1 cut(s) 452
Bsp1286I GDGCHC 1 cut(s) 432
Bsp1407I TGTACA 1 cut(s) 276
BspANI GGCC 1 cut(s) 452
BspCNI CTCAG 4 cut(s) 376, 405, 450, 556
BspLI GGNNCC 1 cut(s) 555
BsrBI CCGCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 357
BsrGI TGTACA 1 cut(s) 276
BssECI CCNNGG 2 cut(s) 201, 456
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 201
Bst6I CTCTTC 2 cut(s) 327, 492
BstACI GRCGYC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 276
BstDEI CTNAG 4 cut(s) 363, 392, 437, 564
BstMAI GTCTC 2 cut(s) 57, 94
BstMWI GCNNNNNNNGC 4 cut(s) 270, 294, 303, 449
BstV1I GCAGC 1 cut(s) 160
BstV2I GAAGAC 3 cut(s) 138, 213, 350
BsuRI GGCC 1 cut(s) 452
BtrI CACGTC 1 cut(s) 399
CaiI CAGNNNCTG 1 cut(s) 563
CseI GACGC 1 cut(s) 39
Csp6I GTAC 1 cut(s) 277
CviAII CATG 2 cut(s) 100, 280
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 4 cut(s) 363, 392, 437, 564
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
Eam1104I CTCTTC 2 cut(s) 327, 492
EarI CTCTTC 2 cut(s) 327, 492
EciI GGCGGA 2 cut(s) 353, 458
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 432
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 2 cut(s) 57, 94
FaeI CATG 2 cut(s) 103, 283
FaiI YATR 4 cut(s) 101, 281, 404, 533
FatI CATG 2 cut(s) 99, 279
FauI CCCGC 3 cut(s) 150, 257, 284
Fnu4HI GCNGC 6 cut(s) 146, 149, 189, 192, 289, 453
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 6 cut(s) 146, 149, 189, 192, 289, 453
GluI GCNGC 6 cut(s) 146, 149, 189, 192, 289, 453
GsuI CTGGAG 1 cut(s) 540
HaeIII GGCC 1 cut(s) 452
HapII CCGG 1 cut(s) 582
HgaI GACGC 1 cut(s) 39
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 2 cut(s) 103, 283
HinfI GANTC 4 cut(s) 38, 71, 107, 182
HpaII CCGG 1 cut(s) 582
HphI GGTGA 1 cut(s) 373
Hpy188I TCNGA 9 cut(s) 46, 106, 130, 160, 175, 226, 366, 418, 565
Hpy188III TCNNGA 1 cut(s) 394
HpyAV CCTTC 6 cut(s) 247, 442, 487, 502, 508, 514
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 3 cut(s) 215, 297, 327
HpyF10VI GCNNNNNNNGC 4 cut(s) 270, 294, 303, 449
HpyF3I CTNAG 4 cut(s) 363, 392, 437, 564
HpySE526I ACGT 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 2 cut(s) 103, 283
LmnI GCTCC 1 cut(s) 559
LpnPI CCDG 6 cut(s) 42, 48, 379, 392, 424, 570
Lsp1109I GCAGC 1 cut(s) 160
LweI GCATC 1 cut(s) 202
MaeII ACGT 1 cut(s) 398
MbiI CCGCTC 1 cut(s) 389
MboII GAAGA 9 cut(s) 143, 188, 191, 218, 254, 344, 355, 473, 509
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 2 cut(s) 75, 536
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 371
MslI CAYNNNNRTG 1 cut(s) 98
MspI CCGG 1 cut(s) 582
MwoI GCNNNNNNNGC 4 cut(s) 270, 294, 303, 449
NlaIII CATG 2 cut(s) 103, 283
NlaIV GGNNCC 1 cut(s) 555
NmeAIII GCCGAG 1 cut(s) 481
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 3 cut(s) 71, 107, 182
PkrI GCNGC 6 cut(s) 147, 150, 190, 193, 290, 454
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PspN4I GGNNCC 1 cut(s) 555
PstNI CAGNNNCTG 1 cut(s) 563
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 1 cut(s) 98
SatI GCNGC 6 cut(s) 146, 149, 189, 192, 289, 453
SchI GAGTC 1 cut(s) 32
SduI GDGCHC 1 cut(s) 432
SetI ASST 8 cut(s) 308, 316, 381, 386, 401, 528, 562, 570
SfaNI GCATC 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 2 cut(s) 75, 536
StyI CCWWGG 1 cut(s) 201
TaiI ACGT 1 cut(s) 401
TaqI TCGA 2 cut(s) 375, 480
TaqII GACCGA 1 cut(s) 83
TasI AATT 2 cut(s) 75, 536
TatI WGTACW 1 cut(s) 276
TauI GCSGC 5 cut(s) 148, 191, 194, 291, 455
TfiI GAWTC 3 cut(s) 71, 107, 182
TseI GCWGC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 296
TspGWI ACGGA 1 cut(s) 76
XmnI GAANNNNTTC 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.