Rroxscaffold_3G00242470

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004333
Physical Location & Seq
Reverse (-)
34785822 .. 34786235
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242470.1

Sequence Viewer

Length: 414 bp
ATGGGAGGCGCTGCCATCACAACCGAACCTGACAACTCCACGCTTCCGCTGCCTTCTCAGAAAAATGACGGCCAATCAGTGAATGTCAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGAAAGGTGAGTGTGGGTTTTGCTTATTCATGAAAGCAGGTCCGTGTGGAGAGAGATTCACAGCTTGGGAACAGTGCGTTAAAGAGTCTGAGAAGAACAAGGATTATGCTGCTGCAAAGTGTGTTGAGGTCTTCAAAACTTTGGATGAGTGTATGAAGGAGAATCAAGATTACTATGATTTTTTCTTGAAGGCCGAGAAAGCAGAATTGGAGAAGGAAGAGGATTCGATGAGTGAGAGCTCAGAGCACAAAGCCAGTTCAATTGGGGATTCTACTGACAAGCAAGAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

137

Amino Acids

15.38

Weight (kDa)

4.29

Isoelectric Point (pI)

80.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 43 - 112 1.5e-24 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 149
AciI CCGC 1 cut(s) 47
AcoI YGGCCR 1 cut(s) 70
AgsI TTSAA 3 cut(s) 256, 310, 381
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 2 cut(s) 185, 360
AluI AGCT 2 cut(s) 185, 360
Alw21I GWGCWC 2 cut(s) 362, 369
AoxI GGCC 2 cut(s) 70, 312
ApeKI GCWGC 4 cut(s) 11, 49, 230, 233
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 1 cut(s) 140
AvaII GGWCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 362
BbsI GAAGAC 1 cut(s) 244
Bbv12I GWGCWC 2 cut(s) 362, 369
BbvI GCAGC 3 cut(s) 36, 217, 220
BccI CCATC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 85
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 149
BisI GCNGC 4 cut(s) 12, 50, 231, 234
BlsI GCNGC 4 cut(s) 13, 51, 232, 235
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 244
Bse1I ACTGG 1 cut(s) 375
BseGI GGATG 1 cut(s) 271
BseMII CTCAG 3 cut(s) 71, 201, 375
BseNI ACTGG 1 cut(s) 375
BseXI GCAGC 3 cut(s) 36, 217, 220
BshFI GGCC 2 cut(s) 72, 314
BsiHKAI GWGCWC 2 cut(s) 362, 369
BsnI GGCC 2 cut(s) 72, 314
Bsp1286I GDGCHC 2 cut(s) 362, 369
BspACI CCGC 1 cut(s) 47
BspANI GGCC 2 cut(s) 72, 314
BspCNI CTCAG 3 cut(s) 70, 202, 374
BspHI TCATGA 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 149
BsrI ACTGG 1 cut(s) 375
Bst4CI ACNGT 1 cut(s) 195
Bst6I CTCTTC 2 cut(s) 114, 333
BstDEI CTNAG 3 cut(s) 57, 210, 361
BstF5I GGATG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstMWI GCNNNNNNNGC 2 cut(s) 49, 320
BstV1I GCAGC 3 cut(s) 36, 217, 220
BstV2I GAAGAC 1 cut(s) 244
BsuRI GGCC 2 cut(s) 72, 314
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 84, 200
BveI ACCTGC 1 cut(s) 149
CciI TCATGA 1 cut(s) 150
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 161
CviAII CATG 1 cut(s) 151
CviJI RGCY 5 cut(s) 72, 185, 314, 360, 374
CviKI_1 RGCY 5 cut(s) 72, 185, 314, 360, 374
DdeI CTNAG 3 cut(s) 57, 210, 361
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 2 cut(s) 114, 333
EarI CTCTTC 2 cut(s) 114, 333
Ecl136II GAGCTC 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 362
FaeI CATG 1 cut(s) 154
FaiI YATR 4 cut(s) 152, 228, 275, 297
FatI CATG 1 cut(s) 150
Fnu4HI GCNGC 4 cut(s) 12, 50, 231, 234
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 4 cut(s) 12, 50, 231, 234
GlaI GCGC 1 cut(s) 10
GluI GCNGC 4 cut(s) 12, 50, 231, 234
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 72, 314
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 154
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 5 cut(s) 177, 206, 283, 344, 389
HphI GGTGA 1 cut(s) 140
Hpy188I TCNGA 3 cut(s) 60, 211, 364
Hpy188III TCNNGA 3 cut(s) 151, 287, 307
HpyAV CCTTC 5 cut(s) 63, 271, 304, 328, 401
HpyCH4III ACNGT 1 cut(s) 195
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 320
HpyF3I CTNAG 3 cut(s) 57, 210, 361
Hsp92II CATG 1 cut(s) 154
HspAI GCGC 1 cut(s) 9
LpnPI CCDG 3 cut(s) 42, 144, 388
Lsp1109I GCAGC 3 cut(s) 36, 217, 220
MfeI CAATTG 1 cut(s) 381
MhlI GDGCHC 2 cut(s) 362, 369
MluCI AATT 2 cut(s) 326, 381
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 2 cut(s) 241, 334
MseI TTAA 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 49
MunI CAATTG 1 cut(s) 381
MwoI GCNNNNNNNGC 2 cut(s) 49, 320
NlaIII CATG 1 cut(s) 154
NmeAIII GCCGAG 1 cut(s) 340
PagI TCATGA 1 cut(s) 150
PfeI GAWTC 4 cut(s) 177, 283, 344, 389
PkrI GCNGC 4 cut(s) 13, 51, 232, 235
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 362
PspPI GGNCC 1 cut(s) 161
SacI GAGCTC 1 cut(s) 362
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 4 cut(s) 12, 50, 231, 234
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 1 cut(s) 215
SduI GDGCHC 2 cut(s) 362, 369
SetI ASST 7 cut(s) 31, 130, 163, 187, 252, 362, 412
SinI GGWCC 1 cut(s) 161
Sse9I AATT 2 cut(s) 326, 381
SsiI CCGC 1 cut(s) 47
SstI GAGCTC 1 cut(s) 362
TaaI ACNGT 1 cut(s) 195
TaqI TCGA 1 cut(s) 347
TasI AATT 2 cut(s) 326, 381
TfiI GAWTC 4 cut(s) 177, 283, 344, 389
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 2 cut(s) 84, 200
TseI GCWGC 4 cut(s) 11, 49, 230, 233
TspDTI ATGAA 3 cut(s) 139, 167, 290
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 2 cut(s) 84, 200
VpaK11BI GGWCC 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.