RchiOBHm_Chr7g0217291

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004333
Physical Location & Seq
Forward (+)
35158112 .. 35161227
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19445

Sequence Viewer

Length: 426 bp
ATGGGAGGCGCTTCCATCACAACCGAACCTAACTCTTCCAACTCCACACTTCCGCTGCCTTCTCAGAAAAATGATGGCCAATCAGAGAATGGCAAAGAAGAAGAAGAAGAAAAAGAAGAAGATGCAGAGGAAGAGAAAGGTGAGTGTGGGTTTTGCTTATTCATGAAAGCAGGTCCGTGTGGAGAGAGATTCACAGCTTGGGAACAGTGCGTTAAAGAGTCTGAGAAGAACAAGGATTATGCTGCTGCAAAGTGTGTTGAGGTCTTCAAAACTTTGGATGAGTGTATGAAAGAGAATCAAGCTTACTATGATTTTTTCTTGAGGGCCGAGAAAGCAGAATTGGAGAAGGAAGAGGATTTGATGAGAGAGAGCTCAGAGCACAAAGCCAGTTCAATTGGGGATTCTACTGACAAGCAAGAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

141

Amino Acids

15.82

Weight (kDa)

4.42

Isoelectric Point (pI)

68.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 47 - 116 6.3e-24 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 161
AciI CCGC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 76
AgsI TTSAA 2 cut(s) 268, 393
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 3 cut(s) 197, 302, 372
AluI AGCT 3 cut(s) 197, 302, 372
Alw21I GWGCWC 2 cut(s) 374, 381
AoxI GGCC 2 cut(s) 76, 324
ApeKI GCWGC 3 cut(s) 55, 242, 245
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 173, 324
AsuHPI GGTGA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 173
BalI TGGCCA 1 cut(s) 78
BanII GRGCYC 1 cut(s) 374
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 2 cut(s) 374, 381
BbvI GCAGC 3 cut(s) 42, 229, 232
BccI CCATC 2 cut(s) 23, 68
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 3 cut(s) 56, 243, 246
BlsI GCNGC 3 cut(s) 57, 244, 247
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 173, 324
BmsI GCATC 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 340
Bse1I ACTGG 1 cut(s) 387
BseGI GGATG 1 cut(s) 283
BseMII CTCAG 3 cut(s) 77, 213, 387
BseNI ACTGG 1 cut(s) 387
BseXI GCAGC 3 cut(s) 42, 229, 232
BshFI GGCC 2 cut(s) 78, 326
BsiHKAI GWGCWC 2 cut(s) 374, 381
BsnI GGCC 2 cut(s) 78, 326
Bsp1286I GDGCHC 2 cut(s) 374, 381
BspACI CCGC 1 cut(s) 53
BspANI GGCC 2 cut(s) 78, 326
BspCNI CTCAG 3 cut(s) 76, 214, 386
BspHI TCATGA 1 cut(s) 162
BspMI ACCTGC 1 cut(s) 161
BsrI ACTGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 207
Bst6I CTCTTC 3 cut(s) 40, 126, 345
BstDEI CTNAG 3 cut(s) 63, 222, 373
BstF5I GGATG 1 cut(s) 283
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstMWI GCNNNNNNNGC 1 cut(s) 332
BstV1I GCAGC 3 cut(s) 42, 229, 232
BstV2I GAAGAC 1 cut(s) 256
BsuRI GGCC 2 cut(s) 78, 326
BtsCI GGATG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 212
BveI ACCTGC 1 cut(s) 161
CciI TCATGA 1 cut(s) 162
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 173, 324
CviAII CATG 1 cut(s) 163
CviJI RGCY 6 cut(s) 78, 197, 302, 326, 372, 386
CviKI_1 RGCY 6 cut(s) 78, 197, 302, 326, 372, 386
DdeI CTNAG 3 cut(s) 63, 222, 373
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 3 cut(s) 40, 126, 345
EarI CTCTTC 3 cut(s) 40, 126, 345
Ecl136II GAGCTC 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 374
Eco47I GGWCC 1 cut(s) 173
Eco53kI GAGCTC 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 372
EcoT38I GRGCYC 1 cut(s) 374
FaeI CATG 1 cut(s) 166
FaiI YATR 4 cut(s) 164, 240, 287, 309
FatI CATG 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 56, 243, 246
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 374
Fsp4HI GCNGC 3 cut(s) 56, 243, 246
GlaI GCGC 1 cut(s) 10
GluI GCNGC 3 cut(s) 56, 243, 246
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 78, 326
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 166
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 4 cut(s) 189, 218, 295, 401
HphI GGTGA 1 cut(s) 152
Hpy188I TCNGA 4 cut(s) 66, 85, 223, 376
Hpy188III TCNNGA 2 cut(s) 163, 319
HpyAV CCTTC 3 cut(s) 69, 340, 413
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4V TGCA 2 cut(s) 125, 248
HpyF10VI GCNNNNNNNGC 1 cut(s) 332
HpyF3I CTNAG 3 cut(s) 63, 222, 373
Hsp92II CATG 1 cut(s) 166
HspAI GCGC 1 cut(s) 9
LpnPI CCDG 2 cut(s) 156, 400
Lsp1109I GCAGC 3 cut(s) 42, 229, 232
LweI GCATC 1 cut(s) 112
MfeI CAATTG 1 cut(s) 393
MhlI GDGCHC 2 cut(s) 374, 381
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 2 cut(s) 338, 393
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 4 cut(s) 121, 253, 315, 346
Mox20I TGGCCA 1 cut(s) 78
MscI TGGCCA 1 cut(s) 78
MseI TTAA 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 55
MunI CAATTG 1 cut(s) 393
MwoI GCNNNNNNNGC 1 cut(s) 332
NlaIII CATG 1 cut(s) 166
NmeAIII GCCGAG 1 cut(s) 352
PagI TCATGA 1 cut(s) 162
PfeI GAWTC 3 cut(s) 189, 295, 401
PkrI GCNGC 3 cut(s) 57, 244, 247
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 374
PspPI GGNCC 2 cut(s) 173, 324
SacI GAGCTC 1 cut(s) 374
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 3 cut(s) 56, 243, 246
Sau96I GGNCC 2 cut(s) 173, 324
SchI GAGTC 1 cut(s) 227
SduI GDGCHC 2 cut(s) 374, 381
SetI ASST 8 cut(s) 31, 142, 175, 199, 264, 304, 374, 424
SfaNI GCATC 1 cut(s) 112
SgeI CNNG 9 cut(s) 175, 183, 189, 210, 244, 311, 331, 340, 399
SinI GGWCC 1 cut(s) 173
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
Sse9I AATT 2 cut(s) 338, 393
SsiI CCGC 1 cut(s) 53
SstI GAGCTC 1 cut(s) 374
TaaI ACNGT 1 cut(s) 207
TasI AATT 2 cut(s) 338, 393
TfiI GAWTC 3 cut(s) 189, 295, 401
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 212
TseI GCWGC 3 cut(s) 55, 242, 245
TspDTI ATGAA 3 cut(s) 151, 179, 302
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.