Rmu_sc0013923.1_g000003

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013923.1
OG0004333
Physical Location & Seq
Forward (+)
8311 .. 8721
411 bp
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UTR
Exon/CDS
Intron
Rmu_sc0013923.1_g000003.1.cds

Sequence Viewer

Length: 411 bp
atgggaggcgcttccatcacaaccgaatctaactcttccaactccacacttccgctgccttctcagaaaaatgatggccaatcagagaatggcaaagaagaagaagatgcagaggaagagaaaggtgagtgtgggttttgcttattcatgaaagcaggtccgtgtggagagagattcacagcttgggaacagtgcgttaaagagtctgagaagaacaacgattatgctgctgcaaagtgtgttgaggtcttcaaaactttggatgagtgtatgaaagagaatcaagattactatgattttttcttgagggccgagaaagcagacttggagaaggaagaggatttgatgagagagaactcagagcacaaagccagttcaattggggattctactgacaagcaagaaggttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

136

Amino Acids

15.21

Weight (kDa)

4.36

Isoelectric Point (pI)

60.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 146
AciI CCGC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 76
AgsI TTSAA 2 cut(s) 253, 378
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 1 cut(s) 182
AluI AGCT 1 cut(s) 182
Alw21I GWGCWC 1 cut(s) 366
AoxI GGCC 2 cut(s) 76, 309
ApeKI GCWGC 3 cut(s) 55, 227, 230
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 158, 309
AsuHPI GGTGA 1 cut(s) 137
AvaII GGWCC 1 cut(s) 158
BalI TGGCCA 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 42, 214, 217
BccI CCATC 2 cut(s) 23, 68
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 146
BisI GCNGC 3 cut(s) 56, 228, 231
BlsI GCNGC 3 cut(s) 57, 229, 232
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 2 cut(s) 158, 309
BmsI GCATC 1 cut(s) 97
BpiI GAAGAC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 268
BseMII CTCAG 3 cut(s) 77, 198, 372
BseNI ACTGG 1 cut(s) 372
BseXI GCAGC 3 cut(s) 42, 214, 217
BshFI GGCC 2 cut(s) 78, 311
BsiHKAI GWGCWC 1 cut(s) 366
BsnI GGCC 2 cut(s) 78, 311
Bsp1286I GDGCHC 1 cut(s) 366
BspACI CCGC 1 cut(s) 53
BspANI GGCC 2 cut(s) 78, 311
BspCNI CTCAG 3 cut(s) 76, 199, 371
BspHI TCATGA 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 146
BsrI ACTGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 192
Bst6I CTCTTC 3 cut(s) 40, 111, 330
BstDEI CTNAG 3 cut(s) 63, 207, 358
BstF5I GGATG 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstV1I GCAGC 3 cut(s) 42, 214, 217
BstV2I GAAGAC 1 cut(s) 241
BsuRI GGCC 2 cut(s) 78, 311
BtsCI GGATG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 197
BveI ACCTGC 1 cut(s) 146
CciI TCATGA 1 cut(s) 147
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 158, 309
CviAII CATG 1 cut(s) 148
CviJI RGCY 4 cut(s) 78, 182, 311, 371
CviKI_1 RGCY 4 cut(s) 78, 182, 311, 371
DdeI CTNAG 3 cut(s) 63, 207, 358
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 3 cut(s) 40, 111, 330
EarI CTCTTC 3 cut(s) 40, 111, 330
Eco47I GGWCC 1 cut(s) 158
FaeI CATG 1 cut(s) 151
FaiI YATR 4 cut(s) 149, 225, 272, 294
FatI CATG 1 cut(s) 147
Fnu4HI GCNGC 3 cut(s) 56, 228, 231
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 3 cut(s) 56, 228, 231
GlaI GCGC 1 cut(s) 10
GluI GCNGC 3 cut(s) 56, 228, 231
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 78, 311
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 5 cut(s) 26, 174, 203, 280, 386
HphI GGTGA 1 cut(s) 137
Hpy188I TCNGA 4 cut(s) 66, 85, 208, 361
Hpy188III TCNNGA 3 cut(s) 148, 284, 304
HpyAV CCTTC 3 cut(s) 69, 325, 398
HpyCH4III ACNGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 110, 233
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 3 cut(s) 63, 207, 358
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 1 cut(s) 9
LpnPI CCDG 2 cut(s) 141, 385
Lsp1109I GCAGC 3 cut(s) 42, 214, 217
LweI GCATC 1 cut(s) 97
MboII GAAGA 8 cut(s) 27, 110, 113, 116, 128, 223, 241, 347
MfeI CAATTG 1 cut(s) 378
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 4 cut(s) 106, 238, 300, 331
Mox20I TGGCCA 1 cut(s) 78
MscI TGGCCA 1 cut(s) 78
MseI TTAA 1 cut(s) 198
Msp20I TGGCCA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 55
MunI CAATTG 1 cut(s) 378
MwoI GCNNNNNNNGC 1 cut(s) 317
NlaIII CATG 1 cut(s) 151
NmeAIII GCCGAG 1 cut(s) 337
PagI TCATGA 1 cut(s) 147
PfeI GAWTC 4 cut(s) 26, 174, 280, 386
PkrI GCNGC 3 cut(s) 57, 229, 232
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PspPI GGNCC 2 cut(s) 158, 309
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 3 cut(s) 56, 228, 231
Sau96I GGNCC 2 cut(s) 158, 309
SchI GAGTC 1 cut(s) 212
SduI GDGCHC 1 cut(s) 366
SetI ASST 5 cut(s) 127, 160, 184, 249, 409
SfaNI GCATC 1 cut(s) 97
SgeI CNNG 9 cut(s) 160, 168, 174, 195, 296, 316, 325, 337, 384
SinI GGWCC 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
Sse9I AATT 1 cut(s) 378
SsiI CCGC 1 cut(s) 53
TaaI ACNGT 1 cut(s) 192
TasI AATT 1 cut(s) 378
TfiI GAWTC 4 cut(s) 26, 174, 280, 386
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 1 cut(s) 197
TseI GCWGC 3 cut(s) 55, 227, 230
TspDTI ATGAA 3 cut(s) 136, 164, 287
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 158
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.